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Molecular Membrane Biology Volume 25, 2008 - Issue 8: The Membrane Protein Structure Initiative of the BBSRC
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The structure of the efflux pump AcrB in complex with bile acid

David Drew Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London
,
Mirjam M. Klepsch Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
,
Simon Newstead Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London
,
Ralf Flaig Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Chilton, Oxfordshire, UK
,
Jan-Willem De Gier Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden
,
So Iwata Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Chilton, Oxfordshire, UK; Department of Cell Biology, Graduate School of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-Ku, JapanCorrespondence[email protected]
&
Konstantinos Beis Division of Molecular Biosciences, Membrane Protein Crystallography Group and Membrane Protein Laboratory, Imperial College, London; Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Chilton, Oxfordshire, UKCorrespondence[email protected]
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Pages 677-682 | Received 03 Sep 2008, Published online: 09 Jul 2009

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Jun Fang, Linliang Yu, Maria Wu & Yinan Wei. (2013) Dissecting the function of a protruding loop in AcrB trimerization. Journal of Biomolecular Structure and Dynamics 31:4, pages 385-392.
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Ling Yang, Haiyang Shi, Lijuan Zhang, Xiaoling Lin, Yinan Wei, Hongxia Jiang & Zhenling Zeng. (2021) Emergence of Two AcrB Substitutions Conferring Multidrug Resistance to Salmonella spp. . Antimicrobial Agents and Chemotherapy 65:5.
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Christopher E. Morgan, Przemyslaw Glaza, Inga V. Leus, Anhthu Trinh, Chih-Chia Su, Meng Cui, Helen I. Zgurskaya & Edward W. Yu. (2021) Cryoelectron Microscopy Structures of AdeB Illuminate Mechanisms of Simultaneous Binding and Exporting of Substrates. mBio 12:1.
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James Birch, Harish Cheruvara, Nadisha Gamage, Peter J. Harrison, Ryan Lithgo & Andrew Quigley. (2020) Changes in Membrane Protein Structural Biology. Biology 9:11, pages 401.
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Ekta Rathi, Avinash Kumar & Suvarna G. Kini. (2020) Computational approaches in efflux pump inhibitors: current status and prospects. Drug Discovery Today 25:10, pages 1883-1890.
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Chih-Chia Su, Christopher E. Morgan, Sekhar Kambakam, Malligarjunan Rajavel, Harry Scott, Wei Huang, Corey C. Emerson, Derek J. Taylor, Phoebe L. Stewart, Robert A. Bonomo & Edward W. Yu. (2019) Cryo-Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump. mBio 10:4.
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Keisuke Sakurai, Seiji Yamasaki, Kaori Nakao, Kunihiko Nishino, Akihito Yamaguchi & Ryosuke Nakashima. (2019) Crystal structures of multidrug efflux pump MexB bound with high-molecular-mass compounds. Scientific Reports 9:1.
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Ryosuke Nakashima, Keisuke Sakurai & Akihito Yamaguchi. 2018. Bacterial Multidrug Exporters. Bacterial Multidrug Exporters 25 36 .
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Christine Oswald, Heng-Keat Tam & Klaas M. Pos. (2016) Transport of lipophilic carboxylates is mediated by transmembrane helix 2 in multidrug transporter AcrB. Nature Communications 7:1.
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Shirin Jamshidi, J Mark Sutton & Khondaker M Rahman. (2016) An overview of bacterial efflux pumps and computational approaches to study efflux pump inhibitors. Future Medicinal Chemistry 8:2, pages 195-210.
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Sergey Boyarskiy, Stephanie Davis López, Niwen Kong & Danielle Tullman-Ercek. (2016) Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n-butanol. Metabolic Engineering 33, pages 130-137.
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Zhicheng Zuo, Beibei Wang, Jingwei Weng & Wenning Wang. (2015) Stepwise substrate translocation mechanism revealed by free energy calculations of doxorubicin in the multidrug transporter AcrB. Scientific Reports 5:1.
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Xian-Zhi Li, Patrick Plésiat & Hiroshi Nikaido. (2015) The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria. Clinical Microbiology Reviews 28:2, pages 337-418.
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V. Ramakrishnan. (2015) The Diamond Light Source and the challenges ahead for structural biology: some informal remarks. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 373:2036, pages 20130156.
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Wei Lu, Meng Zhong, Qian Chai, Zhaoshuai Wang, Linliang Yu & Yinan Wei. (2014) Functional Relevance of AcrB Trimerization in Pump Assembly and Substrate Binding. PLoS ONE 9:2, pages e89143.
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Alfred D. Kinana, Attilio V. Vargiu & Hiroshi Nikaido. (2013) Some Ligands Enhance the Efflux of Other Ligands by the Escherichia coli Multidrug Pump AcrB . Biochemistry 52:46, pages 8342-8351.
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Xin-Qiu Yao, Nobuhiro Kimura, Satoshi Murakami & Shoji Takada. (2013) Drug Uptake Pathways of Multidrug Transporter AcrB Studied by Molecular Simulations and Site-Directed Mutagenesis Experiments. Journal of the American Chemical Society 135:20, pages 7474-7485.
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Jadwiga Handzlik, Anna Matys & Katarzyna Kieć-Kononowicz. (2013) Recent Advances in Multi-Drug Resistance (MDR) Efflux Pump Inhibitors of Gram-Positive Bacteria S. aureus. Antibiotics 2:1, pages 28-45.
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Nadine Fischer & Christian Kandt. (2013) Porter domain opening and closing motions in the multi-drug efflux transporter AcrB. Biochimica et Biophysica Acta (BBA) - Biomembranes 1828:2, pages 632-641.
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Anna Hjelm, Susan Schlegel, Thomas Baumgarten, Mirjam Klepsch, David Wickström, David Drew & Jan-Willem de Gier. 2013. Membrane Biogenesis. Membrane Biogenesis 381 400 .
Wei Lu, Qian Chai, Meng Zhong, Linliang Yu, Jun Fang, Tong Wang, Huilin Li, Haining Zhu & Yinan Wei. (2012) Assembling of AcrB Trimer in Cell Membrane. Journal of Molecular Biology 423:1, pages 123-134.
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Shen Geng, Jun Fang, Kendrick B. Turner, Sylvia Daunert & Yinan Wei. (2012) Accumulation and efflux of polychlorinated biphenyls in Escherichia coli. Analytical and Bioanalytical Chemistry 403:8, pages 2403-2409.
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Thomas Eicher, Hi-jea ChaMarkus A. SeegerLorenz Brandstätter, Jasmin El-DelikJürgen A. Bohnert, Winfried V. Kern, François Verrey, Markus G. GrütterKay Diederichs & Klaas M. Pos. (2012) Transport of drugs by the multidrug transporter AcrB involves an access and a deep binding pocket that are separated by a switch-loop. Proceedings of the National Academy of Sciences 109:15, pages 5687-5692.
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Hiroshi Nikaido & Jean-Marie Pagès. (2012) Broad-specificity efflux pumps and their role in multidrug resistance of Gram-negative bacteria. FEMS Microbiology Reviews 36:2, pages 340-363.
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Linliang Yu, Wei Lu & Yinan Wei. (2011) AcrB Trimer Stability and Efflux Activity, Insight from Mutagenesis Studies. PLoS ONE 6:12, pages e28390.
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Ryosuke Nakashima, Keisuke Sakurai, Seiji Yamasaki, Kunihiko Nishino & Akihito Yamaguchi. (2011) Structures of the multidrug exporter AcrB reveal a proximal multisite drug-binding pocket. Nature 480:7378, pages 565-569.
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Jean-Marie Pagès, Laura Monlezun, Isabelle Broutin & Anne Davin-Regli. (2011) Les mécanismes d’efflux et la résistance chez Pseudomonas aeruginosa. Revue Francophone des Laboratoires 2011:435, pages 63-72.
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Sarah J. QuillinKierstyn T. SchwartzJess H. Leber. (2011) The novel Listeria monocytogenes bile sensor BrtA controls expression of the cholic acid efflux pump MdrT . Molecular Microbiology 81:1, pages 129-142.
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Elizabeth A. Trainor, Katherine E. Horton, Paul B. Savage, Traci L. Testerman & David J. McGee. (2011) Role of the HefC Efflux Pump in Helicobacter pylori Cholesterol-Dependent Resistance to Ceragenins and Bile Salts . Infection and Immunity 79:1, pages 88-97.
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Fasahath Husain & Hiroshi Nikaido. (2010) Substrate path in the AcrB multidrug efflux pump of Escherichia coli. Molecular Microbiology 78:2, pages 320-330.
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Gilles Phan, Houssain Benabdelhak, Marie-Bernard Lascombe, Philippe Benas, Stéphane Rety, Martin Picard, Arnaud Ducruix, Catherine Etchebest & Isabelle Broutin. (2010) Structural and Dynamical Insights into the Opening Mechanism of P. aeruginosa OprM Channel. Structure 18:4, pages 507-517.
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Thomas Eicher, Lorenz Brandstätter & Klaas M. Pos. (2009) Structural and functional aspects of the multidrug efflux pump AcrB. bchm 390:8, pages 693-699.
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