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Original Articles

Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications

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Pages 369-395 | Published online: 23 Oct 2006

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X. Michalet, O. H. W. Siegmund, J. V. Vallerga, P. Jelinsky, J. E. Millaud & S. Weiss. (2007) Detectors for single-molecule fluorescence imaging and spectroscopy. Journal of Modern Optics 54:2-3, pages 239-281.
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Victoria E. Centonze, Akiyuki Takahashi, Elizabeth Casanova & Brian Herman. (2000) Quantitative Fluorescence Microscopy. Journal of Histotechnology 23:3, pages 229-234.
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Articles from other publishers (101)

Sacha Escamez, Christine Terryn, Madhavi Latha Gandla, Zakiya Yassin, Gerhard Scheepers, Torgny Näsholm, Ola Sundman, Leif J. Jönsson, Judith Lundberg-Felten, Hannele Tuominen, Totte Niittylä & Gabriel Paës. (2021) Fluorescence Lifetime Imaging as an In Situ and Label-Free Readout for the Chemical Composition of Lignin . ACS Sustainable Chemistry & Engineering 9:51, pages 17381-17392.
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Lucas Becker, Nicole Janssen, Shannon L. Layland, Thomas E. Mürdter, Anne T. Nies, Katja Schenke-Layland & Julia Marzi. (2021) Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring. Cancers 13:22, pages 5682.
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Yanfeng Liu, Yiwan Song, Jia Zhang, Zhigang Yang, Xiao Peng, Wei Yan & Junle Qu. (2021) Responsive Carbonized Polymer Dots for Optical Super-resolution and Fluorescence Lifetime Imaging of Nucleic Acids in Living Cells. ACS Applied Materials & Interfaces 13:43, pages 50733-50743.
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Desheng Zhu, Yuanyang Zhao, Yourui Tu, Ruifeng Li, Keyan Liu, Yunkun Zhao, Benli Yu & Liang Lu. (2021) Relaxation characteristics for efficient fluorescence lifetime detection of gain media in the fiber laser. Applied Optics 60:19, pages 5678.
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Ruslan I. Dmitriev, Xavier Intes & Margarida M. Barroso. (2021) Luminescence lifetime imaging of three-dimensional biological objects. Journal of Cell Science 134:9, pages 1-17.
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Md Abdul Kader Sagar, Kevin P. Cheng, Jonathan N. Ouellette, Justin C. Williams, Jyoti J. Watters & Kevin W. Eliceiri. (2020) Machine Learning Methods for Fluorescence Lifetime Imaging (FLIM) Based Label-Free Detection of Microglia. Frontiers in Neuroscience 14.
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R. LI, A. LIU, T. WU, W. XIAO, LI TANG & LINGLING CHEN. (2020) Digital scanned laser light‐sheet fluorescence lifetime microscopy with wide‐field time‐gated imaging. Journal of Microscopy 279:1, pages 69-76.
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Liisa M. Hirvonen & Klaus Suhling. (2020) Fast Timing Techniques in FLIM Applications. Frontiers in Physics 8.
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Shuntaro Ito, Masaaki Hashimoto & Yoshihiro Taguchi. (2020) Development of a Robust Autofluorescence Lifetime Sensing Method for Use in an Endoscopic Application. Sensors 20:7, pages 1847.
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Pavel Banerjee, Arghajit Pyne & Nilmoni Sarkar. (2020) Understanding the Self-Assembling Behavior of Biological Building Block Molecules: A Spectroscopic and Microscopic Approach. The Journal of Physical Chemistry B 124:11, pages 2065-2080.
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Pavel Banerjee, Dipankar Mondal, Meghna Ghosh, Devdeep Mukherjee, Pratyush Kiran Nandi, Tapas Kumar Maiti & Nilmoni Sarkar. (2020) Selective Self-Assembly of 5-Fluorouracil through Nonlinear Solvent Response Modulates Membrane Dynamics. Langmuir 36:10, pages 2707-2719.
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Xianhui Lian, Ming-Yuan Wei & Qiang Ma. (2019) Nanomedicines for Near-Infrared Fluorescent Lifetime-Based Bioimaging. Frontiers in Bioengineering and Biotechnology 7.
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Julia Preiss, Daniel Kage, Katrin Hoffmann, Todd J. Martínez, Ute Resch-Genger & Martin Presselt. (2018) Ab Initio Prediction of Fluorescence Lifetimes Involving Solvent Environments by Means of COSMO and Vibrational Broadening. The Journal of Physical Chemistry A 122:51, pages 9813-9820.
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Mira Sibai, Hussein Mehidine, Fanny Poulon, Ali Ibrahim, P. Varlet, M. Juchaux, J. Pallud, B. Devaux, A. Kudlinski & Darine Abi Haidar. (2018) The Impact of Compressed Femtosecond Laser Pulse Durations on Neuronal Tissue Used for Two-Photon Excitation Through an Endoscope. Scientific Reports 8:1.
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Fabio Acerbi & Matteo Perenzoni. 2018. Photon Counting - Fundamentals and Applications. Photon Counting - Fundamentals and Applications.
Jiangtao Xu, An Zhang, Zhiyuan Gao, Kaiming Nie & Jun Qiao. (2017) A low data-rate fluorescence lifetime imaging system with CMM pre-processed in-pixel. Microelectronics Journal 69, pages 28-34.
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Mathijs W. H. Garming, I. Gerward C. Weppelman, Pascal de Boer, Felipe Perona Martínez, Romana Schirhagl, Jacob P. Hoogenboom & Robert J. Moerland. (2017) Nanoparticle discrimination based on wavelength and lifetime-multiplexed cathodoluminescence microscopy. Nanoscale 9:34, pages 12727-12734.
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Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregidgo, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon P. Poland, Simao Coelho, Robert Henderson & Nikola Krstajic. 2017. Handbook of Photonics for Biomedical Engineering. Handbook of Photonics for Biomedical Engineering 353 405 .
Michael Schäferling. 2007. Encyclopedia of Analytical Chemistry. Encyclopedia of Analytical Chemistry 1 52 .
. (2016) A Pixel Analog-Counting Single-Photon Imager With Time-Gating and Self-Referenced Column-Parallel A/D Conversion for Fluorescence Lifetime Imaging. IEEE Journal of Solid-State Circuits 51:1, pages 155-167.
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Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregidgo, Alix Le Marois, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon Poland, Simao Coelho, Robert Henderson & Nikola Krstajic. (2015) Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments. Medical Photonics 27, pages 3-40.
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David J. S. Birch, Yu Chen & Olaf J. Rolinski. 2015. Photonics. Photonics 1 58 .
Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregidgo, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon Poland, Simao Coelho, Robert Henderson & Nikola Krstajic. 2014. Handbook of Photonics for Biomedical Engineering. Handbook of Photonics for Biomedical Engineering 1 50 .
Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregido, Alix le Marois, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon Poland, Simon Coelho & Richard Dimble. 2015. Advanced Time-Correlated Single Photon Counting Applications. Advanced Time-Correlated Single Photon Counting Applications 119 188 .
Richard Hallworth. 2014. Natural Biomarkers for Cellular Metabolism. Natural Biomarkers for Cellular Metabolism 77 106 .
Joseph Lakowicz. 2014. Biomedical Photonics Handbook, Second Edition. Biomedical Photonics Handbook, Second Edition 353 396 .
Ryan M. Field, Simeon Realov & Kenneth L. Shepard. (2014) A 100 fps, Time-Correlated Single-Photon-Counting-Based Fluorescence-Lifetime Imager in 130 nm CMOS. IEEE Journal of Solid-State Circuits 49:4, pages 867-880.
Crossref
Alex Marki, Eugeny Ermilov, Andreas Zakrzewicz, Akos Koller, Timothy W. Secomb & Axel R. Pries. (2013) Tracking of fluorescence nanoparticles with nanometre resolution in a biological system: assessing local viscosity and microrheology. Biomechanics and Modeling in Mechanobiology 13:2, pages 275-288.
Crossref
René Ebrecht, Craig Don Paul & Fred S. Wouters. (2014) Fluorescence lifetime imaging microscopy in the medical sciences. Protoplasma 251:2, pages 293-305.
Crossref
M.A. Markus, C. Dullin & F. Alves. 2014. Comprehensive Biomedical Physics. Comprehensive Biomedical Physics 227 243 .
Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregidgo, Dmitri Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon Poland, Simon Coelho, Robert Henderson & Nikola Krstajic. 2021. Handbook of Photonics for Biomedical Engineering. Handbook of Photonics for Biomedical Engineering 1 50 .
Leng-Chun Chen, William R. LloydIIIIII, Ching-Wei Chang, Dhruv Sud & Mary-Ann Mycek. 2013. Digital Microscopy. Digital Microscopy 457 488 .
Arne S. Kristoffersen, Øyvind Svensen, Nicolausi Ssebiyonga, Svein R. Erga, Jakob J. Stamnes & Øyvind Frette. (2012) Chlorophyll a and NADPH Fluorescence Lifetimes in the Microalgae Haematococcus pluvialis (Chlorophyceae) under Normal and Astaxanthin-Accumulating Conditions . Applied Spectroscopy 66:10, pages 1216-1225.
Crossref
W. BECKER. (2012) Fluorescence lifetime imaging – techniques and applications. Journal of Microscopy 247:2, pages 119-136.
Crossref
Ryan A. Colyer, Oswald H. W. Siegmund, Anton S. Tremsin, John V. Vallerga, Shimon Weiss & Xavier Michalet. (2012) Phasor imaging with a widefield photon-counting detector. Journal of Biomedical Optics 17:1, pages 016008.
Crossref
Michael Schäferling. 2007. Encyclopedia of Analytical Chemistry. Encyclopedia of Analytical Chemistry.
James McGinty, Harriet B. Taylor, Lingling Chen, Laurence Bugeon, Jonathan R. Lamb, Margaret J. Dallman & Paul M. W. French. (2011) In vivo fluorescence lifetime optical projection tomography. Biomedical Optics Express 2:5, pages 1340.
Crossref
David G. Buschke, Derek J. Hei, Kevin W. Eliceiri & Brenda M. Ogle. 2010. Stem Cell-Based Tissue Repair. Stem Cell-Based Tissue Repair 45 80 .
Daniel Doerr, Martin Stark, Friederike Ehrhart, Heiko Zimmermann & Frank Stracke. (2009) Multiphoton microscopy for the in‐situ investigation of cellular processes and integrity in cryopreservation . Biotechnology Journal 4:8, pages 1215-1220.
Crossref
Yuansheng Sun, James N. Demas & Ammasi Periasamy. 2009. Flim Microscopy in Biology and Medicine. Flim Microscopy in Biology and Medicine 91 114 .
Steven S. Vogel, Christopher Thaler, Paul S. Blank & Srinagesh V. Koushik. 2009. Flim Microscopy in Biology and Medicine. Flim Microscopy in Biology and Medicine 245 290 .
Steven S. Vogel, Paul S. Blank, Srinagesh V. Koushik & Christopher Thaler. 2009. Fret and Flim Techniques. Fret and Flim Techniques 351 394 .
James McGinty, Khadija B. Tahir, Romain Laine, Clifford B. Talbot, Christopher Dunsby, Mark A. A. Neil, Laura Quintana, James Swoger, James Sharpe & Paul M. W. French. (2008) Fluorescence lifetime optical projection tomography. Journal of Biophotonics 1:5, pages 390-394.
Crossref
D.K. BIRD, A.L. SCHNEIDER, A.C. WATKINSON, B. FINNIN & T.A. SMITH. (2008) Navigating transdermal diffusion with multiphoton fluorescence lifetime imaging. Journal of Microscopy 230:1, pages 61-69.
Crossref
J.-A. SPITZ, R. YASUKUNI, N. SANDEAU, M. TAKANO, J.-J. VACHON, R. MEALLET-RENAULT & R.B. PANSU. (2008) Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy. Journal of Microscopy 229:1, pages 104-114.
Crossref
Katrin Hoffmann, Ute Resch-Genger & Roland Nitschke. 2008. Standardization and Quality Assurance in Fluorescence Measurements II. Standardization and Quality Assurance in Fluorescence Measurements II 89 116 .
DAMIAN K. BIRD, KENT M. AGG, NEIL W. BARNETT & TREVOR A. SMITH. (2007) Time-resolved fluorescence microscopy of gunshot residue: an application to forensic science. Journal of Microscopy 226:1, pages 18-25.
Crossref
Harry Hochheiser, Kevin W. Eliceiri & Ilya G. Goldberg. 2007. Bioinformatics‐From Genomes to Therapies. Bioinformatics‐From Genomes to Therapies 1573 1626 .
Ching‐Wei Chang, Dhruv Sud & Mary‐Ann Mycek. 2007. Digital Microscopy, 3rd Edition. Digital Microscopy, 3rd Edition 495 524 .
Srinagesh V. Koushik, Huanmian Chen, Christopher Thaler, Henry L. PuhlIIIIII & Steven S. Vogel. (2006) Cerulean, Venus, and VenusY67C FRET Reference Standards. Biophysical Journal 91:12, pages L99-L101.
Crossref
D. K. BIRD, K. W. ELICEIRI & J. G. WHITE. (2006) Optimized temporal response in multichannel two-photon fluorescence lifetime microscopy using a photonic crystal fibre. Journal of Microscopy 224:3, pages 249-255.
Crossref
D. ChorvatJrJr & A. Chorvatova. (2006) Spectrally resolved time-correlated single photon counting: a novel approach for characterization of endogenous fluorescence in isolated cardiac myocytes. European Biophysics Journal 36:1, pages 73-83.
Crossref
E. H. CHO & S. J. LOCKETT. (2006) Calibration and standardization of the emission light path of confocal microscopes. Journal of Microscopy 223:1, pages 15-25.
Crossref
Michael Schäferling & Stefan Nagl. (2006) Optical technologies for the read out and quality control of DNA and protein microarrays. Analytical and Bioanalytical Chemistry 385:3, pages 500-517.
Crossref
Enrico Gratton & Martin J. vandeVen. 2006. Handbook Of Biological Confocal Microscopy. Handbook Of Biological Confocal Microscopy 80 125 .
Damian K. Bird, Long Yan, Kristin M. Vrotsos, Kevin W. Eliceiri, Emily M. Vaughan, Patricia J. Keely, John G. White & Nirmala Ramanujam. (2005) Metabolic Mapping of MCF10A Human Breast Cells via Multiphoton Fluorescence Lifetime Imaging of the Coenzyme NADH. Cancer Research 65:19, pages 8766-8773.
Crossref
U. Resch-Genger, K. Hoffmann, W. Nietfeld, A. Engel, J. Neukammer, R. Nitschke, B. Ebert & R. Macdonald. (2005) How to Improve Quality Assurance in Fluorometry: Fluorescence-Inherent Sources of Error and Suited Fluorescence Standards. Journal of Fluorescence 15:3, pages 337-362.
Crossref
Klaus Suhling, Paul M. W. French & David Phillips. (2005) Time-resolved fluorescence microscopy. Photochemical & Photobiological Sciences 4:1, pages 13-22.
Crossref
GLEN REDFORD & ROBERT M. CLEGG. 2005. Molecular Imaging. Molecular Imaging 193 226 .
Michael Schäferling. 2005. Frontiers in Chemical Sensors. Frontiers in Chemical Sensors 45 92 .
B. Valeur. 2005. Fluorescence Spectroscopy in Biology. Fluorescence Spectroscopy in Biology 30 48 .
Damian K. Bird, Kevin W. Eliceiri, Ching-Hua Fan & John G. White. (2004) Simultaneous two-photon spectral and lifetime fluorescence microscopy. Applied Optics 43:27, pages 5173.
Crossref
Laurens J. Mets & Jean-David Rochaix. 2004. The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas. The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas 685 703 .
Frank V Bright & Chase A Munson. (2003) Time-resolved fluorescence spectroscopy for illuminating complex systems. Analytica Chimica Acta 500:1-2, pages 71-104.
Crossref
Xavier MichaletAchillefs N. KapanidisTed LaurenceFabien PinaudSoeren DooseMalte PflughoefftShimon Weiss. (2003) The Power and Prospects of Fluorescence Microscopies and Spectroscopies. Annual Review of Biophysics and Biomolecular Structure 32:1, pages 161-182.
Crossref
Paul Uravama & Marv-Ann M\cek. 2003. Handbook of Biomedical Fluorescence. Handbook of Biomedical Fluorescence.
Kevin W. Eliceiri, Ching-Hua Fan, Gary E. Lyons & John G. White. (2003) Analysis of histology specimens using lifetime multiphoton microscopy. Journal of Biomedical Optics 8:3, pages 376.
Crossref
Victoria E. Centonze, Mao Sun, Atsushi Masuda, Hans Gerritsen & Brian Herman. 2003. Biophotonics, Part A. Biophotonics, Part A 542 560 .
Klaus Suhling, Jan Siegel, David Phillips, Paul M.W. French, Sandrine Lévêque-Fort, Stephen E.D. Webb & Daniel M. Davis. (2002) Imaging the Environment of Green Fluorescent Protein. Biophysical Journal 83:6, pages 3589-3595.
Crossref
Fiona E. McCann, Klaus Suhling, Leo M. Carlin, Konstantina Eleme, Sabrina B. Taner, Kumiko Yanagi, Bruno Vanherberghen, Paul M. W. French & Daniel M. Davis. (2002) Imaging immune surveillance by T cells and NK cells. Immunological Reviews 189:1, pages 179-192.
Crossref
W J O'Hagan, M McKenna, D C Sherrington, O J Rolinski & D J S Birch. (2002) MHz LED source for nanosecond fluorescence sensing. Measurement Science and Technology 13:1, pages 84-91.
Crossref
David J. S. Birch & Graham Hungerford. 2002. Topics in Fluorescence Spectroscopy. Topics in Fluorescence Spectroscopy 377 416 .
David J.S Birch. (2001) Multiphoton excited fluorescence spectroscopy of biomolecular systems. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 57:11, pages 2313-2336.
Crossref
Brian Herman, Gerald Gordon, Nupam Mahajan & Victoria Centonze. 2001. Methods in Cellular Imaging. Methods in Cellular Imaging 257 272 .
O. Holub, M.J. Seufferheld, C. Gohlke, Govindjee Govindjee & R.M. Clegg. (2000) Fluorescence Lifetime Imaging (FLI) in Real-Time - a New Technique in Photosynthesis Research. Photosynthetica 38:4, pages 581-599.
Crossref
Frank V. Bright, Siddharth Pandey & Gary A. Baker. 2007. Encyclopedia of Analytical Chemistry. Encyclopedia of Analytical Chemistry.
Joachim Goedhart & Theodorus W. J. Gadella. 2000. Root Hairs. Root Hairs 65 94 .
Stephen A. Stricker & Michael Whitaker. (1999) Confocal laser scanning microscopy of calcium dynamics in living cells. Microscopy Research and Technique 46:6, pages 356-369.
Crossref
Michael J. Stimson, Nicole Haralampus-Grynaviski & John D. Simon. (1999) A unique optical arrangement for obtaining spectrally resolved confocal images. Review of Scientific Instruments 70:8, pages 3351-3354.
Crossref
Edward S. Yeung. (1999) Peer Reviewed: Following Single Cell Dynamics with Native Fluorescence Microscopy. Analytical Chemistry 71:15, pages 522A-529A.
Crossref
Akiyuki Takahashi, Patricia Camacho, James D. Lechleiter & Brian Herman. (1999) Measurement of Intracellular Calcium. Physiological Reviews 79:4, pages 1089-1125.
Crossref
THEODORUS W.J. GADELLAJrJr. 1999. Fluorescent and Luminescent Probes for Biological Activity. Fluorescent and Luminescent Probes for Biological Activity 467 479 .
Wolfgang Rettig, Bernd Strehmel, Sigurd Schrader & Holger SeifertB. Herman, X. F. Wang, P. Wodnicki, A. Perisamy, N. Mahajan, G. Berry & G. Gordon. 1999. Applied Fluorescence in Chemistry, Biology and Medicine. Applied Fluorescence in Chemistry, Biology and Medicine 491 507 .
Fredric S. Fay. 2011. Comprehensive Physiology. Comprehensive Physiology 3 22 .
Peter C. Schneider & Robert M. Clegg. (1997) Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications. Review of Scientific Instruments 68:11, pages 4107-4119.
Crossref
Brian Herman, Pawel Wodnicki, Seongwook Kwon, Ammasi Periasamy, Gerald W. Gordon, Nupam Mahajan & Xue Feng Wang. (1997) Recent developments in monitoring calcium and protein interactions in cells using fluorescence lifetime microscopy. Journal of Fluorescence 7:1, pages 85-91.
Crossref
T.F. Deutsch. (1997) Lasers and optics in health care. Proceedings of the IEEE 85:11, pages 1797-1816.
Crossref
Paul G. Spizzirri, John S. Hill, Stephen B. Kahl & Kenneth P. Ghiggino. (2008) Photophysics and Intracellular Distribution of a Boronated Porphyrin Phototherapeutic Agent. Photochemistry and Photobiology 64:6, pages 975-983.
Crossref
Ammasi Periasamy, Pawel Wodnicki, Xue F. Wang, Seongwook Kwon, Gerald W. Gordon & Brian Herman. (1996) Time-resolved fluorescence lifetime imaging microscopy using a picosecond pulsed tunable dye laser system. Review of Scientific Instruments 67:10, pages 3722-3731.
Crossref
David McLoskey, David J. S. Birch, Alan Sanderson, Klaus Suhling, E. Welch & P. J. Hicks. (1996) Multiplexed single-photon counting. I. A time-correlated fluorescence lifetime camera. Review of Scientific Instruments 67:6, pages 2228-2237.
Crossref
C. G. Morgan & A. C. Mitchell. (1996) Fluorescence lifetime imaging: an emerging technique in fluorescence microscopy. Chromosome Research 4:4, pages 261-263.
Crossref
Henryk Szmacinski & Joseph R. Lakowicz. (1995) Fluorescence lifetime-based sensing and imaging. Sensors and Actuators B: Chemical 29:1-3, pages 16-24.
Crossref
Weihong. Tan, Vladimir. Parpura, Philip G. Haydon & Edward S. Yeung. (2002) Neurotransmitter Imaging in Living Cells Based on Native Fluorescence Detection. Analytical Chemistry 67:15, pages 2575-2579.
Crossref
C. G. Morgan, J. G. Murray & A. C. Mitchell. (1995) Photon correlation system for fluorescence lifetime measurements. Review of Scientific Instruments 66:7, pages 3744-3749.
Crossref
Sam Phimphivong, Silvia Kölchens, Paul L. Edmiston & S. Scott Saavedra. (1995) Time-resolved, total internal reflection fluorescence microscopy of cultured cells using a Tb chelate label. Analytica Chimica Acta 307:2-3, pages 403-417.
Crossref
Xue Feng Wang, K. Florine-Casteel, John J. Lemasters & Brian Herman. (1995) Video fluorescence microscopic techniques to monitor local lipid and phospholipid molecular order and organization in cell membranes during hypoxic injury. Journal of Fluorescence 5:1, pages 71-84.
Crossref
Enrico Gratton & Martin J. vandeVen. 1995. Handbook of Biological Confocal Microscopy. Handbook of Biological Confocal Microscopy 69 97 .
X. Sunney XieRobert C. Dunn. (1994) Probing Single Molecule Dynamics. Science 265:5170, pages 361-364.
Crossref
Peter M. Cooke. (2002) Chemical Microscopy. Analytical Chemistry 66:12, pages 558-594.
Crossref
Pamela A. Diliberto, Xue Feng Wang & Brian Herman. 1994. A Practical Guide to the Study of Calcium in Living Cells. A Practical Guide to the Study of Calcium in Living Cells 243 262 .
Theodorus W.J. GadellaJr.Jr., Thomas M. Jovin & Robert M. Clegg. (1993) Fluorescence lifetime imaging microscopy (FLIM): Spatial resolution of microstructures on the nanosecond time scale. Biophysical Chemistry 48:2, pages 221-239.
Crossref

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