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Invited Review Articles

Preanalytical variables that affect the outcome of cell-free DNA measurements

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Pages 484-507 | Received 11 Dec 2019, Accepted 30 Mar 2020, Published online: 12 May 2020

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (4)

Aliki Ntzifa & Evi Lianidou. (2023) Pre-analytical conditions and implementation of quality control steps in liquid biopsy analysis. Critical Reviews in Clinical Laboratory Sciences 60:8, pages 573-594.
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Sander Bach, Birgit M.M. Wever, Mark A. van de Wiel, Joris D. Veltman, Sayed M.S. Hashemi, Geert Kazemier, Idris Bahce & Renske D.M. Steenbergen. (2022) Dynamics of methylated cell-free DNA in the urine of non-small cell lung cancer patients. Epigenetics 17:10, pages 1057-1069.
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Sally Temraz, Rihab Nasr, Deborah Mukherji, Firas Kreidieh & Ali Shamseddine. (2022) Liquid biopsy derived circulating tumor cells and circulating tumor DNA as novel biomarkers in hepatocellular carcinoma. Expert Review of Molecular Diagnostics 22:5, pages 507-518.
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Daniel Andersson, Helena Kristiansson, Mikael Kubista & Anders Ståhlberg. (2021) Ultrasensitive circulating tumor DNA analysis enables precision medicine: experimental workflow considerations. Expert Review of Molecular Diagnostics 21:3, pages 299-310.
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Abel J. Bronkhorst11 & Stefan Holdenrieder11. (2023) The changing face of circulating tumor DNA (ctDNA) profiling: Factors that shape the landscape of methodologies, technologies, and commercialization. Medizinische Genetik 35:4, pages 201-235.
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Awirut Charoensappakit, Kritsanawan Sae-khow, Pongpera Rattanaliam, Nuntanuj Vutthikraivit, Monvasi Pecheenbuvan, Suwasin Udomkarnjananun & Asada leelahavanichkul. (2023) Cell-free DNA as diagnostic and prognostic biomarkers for adult sepsis: a systematic review and meta-analysis. Scientific Reports 13:1.
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Fatemeh Farshchi & Mohammad Hasanzadeh. (2023) Efficient diagnosis of cancer using biosensing of circulating tumor DNAs(ctDNA): Recent progress and challenges. Microchemical Journal 193, pages 109076.
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Alessandro Pancrazzi, Francesco Bloise, Alice Moncada, Roberta Perticucci, Stefania Vecchietti, Francesca Pompili, Francesca Ricciarini, Silvia Lenzi, Cristina Gatteschi, Sabrina Giusti, Maria Pia Rosito, Sabrina Del Buono, Paola Belardi, Alessandra Bruni, Filippo Borri, Andrea Campione, Lorella Laurini, Rossella Occhini, Loretta Presenti, Viviana Viticchi, Maja Rossi, Sara Bardi, Antonio D'Urso, Simona Dei, Duccio Venezia, Raffaele Scala, Carmelo Bengala, Nicola Libertà Decarli, Andrea Carnevali, Carlo Milandri & Agostino Ognibene. (2023) BL-MOL-AR Project, Preliminary Results about Liquid Biopsy: Molecular Approach Experience and Research Activity in Oncological Settings. Global Medical Genetics 10:03, pages 172-187.
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Mark W. Linder, Jim F. Huggett, Katarina Baluchova, Ettore D. Capoluongo, Deborah A. Payne, Aldo Vacaflores Salinas, Verena Haselmann, Tester Ashavaid, Shiyang Pan & Parviz Ahmad-Nejad. (2023) Results from an IFCC global survey on laboratory practices for the analysis of circulating tumor DNA. Clinica Chimica Acta 547, pages 117398.
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Blake Salfer, Daisy Havo, Skyllar Kuppinger, David T. W. Wong, Feng Li & Liying Zhang. (2023) Evaluating Pre-Analytical Variables for Saliva Cell-Free DNA Liquid Biopsy. Diagnostics 13:10, pages 1665.
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Inga Medina Diaz, Annette Nocon, Stefanie A. E. Held, Makbule Kobilay, Dirk Skowasch, Abel J. Bronkhorst, Vida Ungerer, Johannes Fredebohm, Frank Diehl, Stefan Holdenrieder & Frank Holtrup. (2023) Pre-Analytical Evaluation of Streck Cell-Free DNA Blood Collection Tubes for Liquid Profiling in Oncology. Diagnostics 13:7, pages 1288.
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Guadalupe A. Cifuentes, Adrián Santiago, Lucía Méndez Blanco, María Fueyo, Esther López Martínez, Raquel Soria, Irene Martín López, Pepa Cucarella Beltrán, Pablo Pardo-Coto, David Rodriguez-Rubi, Karla Urquilla, Noelia S. Durán, Rebeca Álvarez, Claudia G. Lago, Andrea Otero, Marta Diñeiro, Raquel Capín, Juan Cadiñanos & Rubén Cabanillas. (2022) Clinical utility of liquid biopsy and integrative genomic profiling in early-stage and oligometastatic cancer patients treated with radiotherapy. British Journal of Cancer 128:5, pages 857-876.
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E. Capoluongo, P. Pisapia, U. Malapelle & G. Troncone. 2023. Liquid Biopsy. Liquid Biopsy 119 202 .
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Ugur Gezer, Abel J. Bronkhorst & Stefan Holdenrieder. (2022) The Clinical Utility of Droplet Digital PCR for Profiling Circulating Tumor DNA in Breast Cancer Patients. Diagnostics 12:12, pages 3042.
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Liron Barnea Slonim, Kathy A. Mangold, Mir B. Alikhan, Nora Joseph, Kalpana S. Reddy, Linda M. Sabatini & Karen L. Kaul. (2022) Cell-free Nucleic Acids in Cancer. Clinics in Laboratory Medicine 42:4, pages 669-686.
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Hua Bao, Xiaoxi Chen, Qian Xiao, Shanshan Yang, Shuyu Wu, Xiaonan Wang, Xue Wu, Kefeng Ding & Yang Shao. (2022) Associations of genome-wide cell-free DNA fragmentation profiles with blood biochemical and hematological parameters in healthy individuals. Genomics 114:6, pages 110504.
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Nornazliya Mohamad, Nur Inani Azizan, Nur Fadhilah Khairil Mokhtar, Shuhaimi Mustafa, Mohd Nasir Mohd Desa & Amalia Mohd Hashim. (2022) Future perspectives on aptamer for application in food authentication. Analytical Biochemistry 656, pages 114861.
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Abel J. Bronkhorst, Vida Ungerer, Angela Oberhofer, Sophie Gabriel, Eleni Polatoglou, Hannah Randeu, Carsten Uhlig, Heiko Pfister, Zsuzsanna Mayer & Stefan Holdenrieder. (2022) New Perspectives on the Importance of Cell-Free DNA Biology. Diagnostics 12:9, pages 2147.
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Lilian Pok Wa ZhongRossa W.K. Chiu. (2022) The Next Frontier in Noninvasive Prenatal Diagnostics: Cell-Free Fetal DNA Analysis for Monogenic Disease Assessment. Annual Review of Genomics and Human Genetics 23:1, pages 413-425.
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Angela Oberhofer, Abel Jacobus Bronkhorst, Vida Ungerer & Stefan Holdenrieder. (2022) Profiling disease and tissue-specific epigenetic signatures in cell-free DNA. Journal of Laboratory Medicine 46:4, pages 283-294.
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Abel Jacobus Bronkhorst, Vida Ungerer, Angela Oberhofer & Stefan Holdenrieder. (2022) The rising tide of cell-free DNA profiling: from snapshot to temporal genome analysis. Journal of Laboratory Medicine 46:4, pages 207-224.
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Corinna Keup, Rainer Kimmig & Sabine Kasimir-Bauer. (2022) Multimodality in liquid biopsy: does a combination uncover insights undetectable in individual blood analytes?. Journal of Laboratory Medicine 46:4, pages 255-264.
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Svetlana Tamkovich, Alexey Tupikin, Anton Kozyakov & Pavel Laktionov. (2022) Size and Methylation Index of Cell-Free and Cell-Surface-Bound DNA in Blood of Breast Cancer Patients in the Contest of Liquid Biopsy. International Journal of Molecular Sciences 23:16, pages 8919.
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Hannah Randeu, Abel J. Bronkhorst, Zsuzsanna Mayer, Angela Oberhofer, Eleni Polatoglou, Volker Heinemann, Michael Haas, Stefan Boeck & Stefan Holdenrieder. (2022) Preanalytical Variables in the Analysis of Mitochondrial DNA in Whole Blood and Plasma from Pancreatic Cancer Patients. Diagnostics 12:8, pages 1905.
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Sakti Chakrabarti, Anup Kumar Kasi, Aparna R. Parikh & Amit Mahipal. (2022) Finding Waldo: The Evolving Paradigm of Circulating Tumor DNA (ctDNA)—Guided Minimal Residual Disease (MRD) Assessment in Colorectal Cancer (CRC). Cancers 14:13, pages 3078.
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Ugur Gezer, Abel J. Bronkhorst & Stefan Holdenrieder. (2022) The Utility of Repetitive Cell-Free DNA in Cancer Liquid Biopsies. Diagnostics 12:6, pages 1363.
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Corinna Keup, Rainer Kimmig & Sabine Kasimir-Bauer. (2022) Combinatorial Power of cfDNA, CTCs and EVs in Oncology. Diagnostics 12:4, pages 870.
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Oleg Tutanov, Tatiana Shtam, Alina Grigor’eva, Alexey Tupikin, Yuri Tsentalovich & Svetlana Tamkovich. (2022) Blood Plasma Exosomes Contain Circulating DNA in Their Crown. Diagnostics 12:4, pages 854.
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Jure Krasic, Lucija Skara, Ana Katusic Bojanac, Monika Ulamec, Davor Jezek, Tomislav Kulis & Nino Sincic. (2022) The utility of cfDNA in TGCT patient management: a systematic review. Therapeutic Advances in Medical Oncology 14, pages 175883592210903.
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Yan Li, Yuanyuan Zheng, Liwei Wu, Jingjing Li, Jie Ji, Qiang Yu, Weiqi Dai, Jiao Feng, Jianye Wu & Chuanyong Guo. (2021) Current status of ctDNA in precision oncology for hepatocellular carcinoma. Journal of Experimental & Clinical Cancer Research 40:1.
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Elmo W. I. Neuberger, Alexandra Brahmer, Tobias Ehlert, Katrin Kluge, Keito F. A. Philippi, Simone C. Boedecker, Julia Weinmann-Menke & Perikles Simon. (2021) Validating quantitative PCR assays for cfDNA detection without DNA extraction in exercising SLE patients. Scientific Reports 11:1.
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Ilana Fox-Fisher, Sheina Piyanzin, Bracha Lea Ochana, Agnes Klochendler, Judith Magenheim, Ayelet Peretz, Netanel Loyfer, Joshua Moss, Daniel Cohen, Yaron Drori, Nehemya Friedman, Michal Mandelboim, Marc E Rothenberg, Julie M Caldwell, Mark Rochman, Arash Jamshidi, Gordon Cann, David Lavi, Tommy Kaplan, Benjamin Glaser, Ruth Shemer & Yuval Dor. (2021) Remote immune processes revealed by immune-derived circulating cell-free DNA. eLife 10.
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Liron Barnea Slonim, Kathy A. Mangold, Mir B. Alikhan, Nora Joseph, Kalpana S. Reddy, Linda M. Sabatini & Karen L. Kaul. (2021) Cell-free Nucleic Acids in Cancer. Advances in Molecular Pathology 4, pages 187-198.
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Estelle Bourbon, Vincent Alcazer, Estelle Cheli, Sarah Huet & Pierre Sujobert. (2021) How to Obtain a High Quality ctDNA in Lymphoma Patients: Preanalytical Tips and Tricks. Pharmaceuticals 14:7, pages 617.
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A. Russo, L. Incorvaia, M. Del Re, U. Malapelle, E. Capoluongo, V. Gristina, M. Castiglia, R. Danesi, M. Fassan, G. Giuffrè, S. Gori, A. Marchetti, N. Normanno, C. Pinto, G. Rossi, D. Santini, A. Sartore-Bianchi, N. Silvestris, P. Tagliaferri, G. Troncone, S. Cinieri & G.D. Beretta. (2021) The molecular profiling of solid tumors by liquid biopsy: a position paper of the AIOM–SIAPEC-IAP–SIBioC–SIC–SIF Italian Scientific Societies. ESMO Open 6:3, pages 100164.
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Abel J. Bronkhorst, Vida Ungerer, Frank Diehl, Philippe Anker, Yuval Dor, Michael Fleischhacker, Peter B. Gahan, Lisa Hui, Stefan Holdenrieder & Alain R. Thierry. (2020) Towards systematic nomenclature for cell-free DNA. Human Genetics 140:4, pages 565-578.
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Zuzana Pös, Ondrej Pös, Jakub Styk, Angelika Mocova, Lucia Strieskova, Jaroslav Budis, Ludevit Kadasi, Jan Radvanszky & Tomas Szemes. (2020) Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes. International Journal of Molecular Sciences 21:22, pages 8634.
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Esther L. Moss, Diviya N. Gorsia, Anna Collins, Pavandeep Sandhu, Nalini Foreman, Anupama Gore, Joey Wood, Christopher Kent, Lee Silcock & David S. Guttery. (2020) Utility of Circulating Tumor DNA for Detection and Monitoring of Endometrial Cancer Recurrence and Progression. Cancers 12:8, pages 2231.
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