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Original Article

Measuring oxidative damage to DNA; HPLC and the comet assay compared

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Pages 609-615 | Received 01 Sep 1998, Published online: 07 Jul 2009

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Eun-Sun Hwang & PhyllisE. Bowen. (2007) DNA Damage, a Biomarker of Carcinogenesis: Its Measurement and Modulation by Diet and Environment. Critical Reviews in Food Science and Nutrition 47:1, pages 27-50.
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Tim Hofer, Hanna L. Karlsson & Lennart Möller. (2006) DNA oxidative damage and strand breaks in young healthy individuals: A gender difference and the role of life style factors. Free Radical Research 40:7, pages 707-714.
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Gunther Guetens, Gert De Boeck, Martin Highley, Allan T. van Oosterom & Ernst A. de Bruijn. (2002) Oxidative DNA Damage: Biological Significance and Methods of Analysis. Critical Reviews in Clinical Laboratory Sciences 39:4-5, pages 331-457.
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Lena Müller, Larissa Rhonda Friederike Schütte, David Bücksteeg, Julian Alfke, Thomas Uebel & Melanie Esselen. (2021) Topoisomerase poisoning by the flavonoid nevadensin triggers DNA damage and apoptosis in human colon carcinoma HT29 cells. Archives of Toxicology 95:12, pages 3787-3802.
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Aida El Makawy, Fawzy Eissa, Mahmoud EL-Bamby & Osama Elhamalawy. (2018) Flaxseed oil as a protective agent against bisphenol-A deleterious effects in male mice. Bulletin of the National Research Centre 42:1.
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Pengcheng Li, Grant A Ramm & Graeme A Macdonald. (2016) Value of the 8-oxodG/dG ratio in chronic liver inflammation of patients with hepatocellular carcinoma. Redox Biology 8, pages 259-270.
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A. Paini, G. Scholz, M. Marin-Kuan, B. Schilter, J. O'Brien, P. J. van Bladeren & I. M. C. M. Rietjens. (2011) Quantitative comparison between in vivo DNA adduct formation from exposure to selected DNA-reactive carcinogens, natural background levels of DNA adduct formation and tumour incidence in rodent bioassays. Mutagenesis 26:5, pages 605-618.
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Thomas J. AnchordoquyMarion C. Molina. (2007) Preservation of DNA. Cell Preservation Technology 5:4, pages 180-188.
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G. Koppen, G. Verheyen, A. Maes, U. Van Gorp, G. Schoeters, E. Den Hond, J. Staessen, T. Nawrot, H. A. Roels, R. Vlietinck & L. Verschaeve. (2007) A battery of DNA effect biomarkers to evaluate environmental exposure of Flemish adolescents. Journal of Applied Toxicology 27:3, pages 238-246.
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A. Pilger & H. W. Rüdiger. (2006) 8-Hydroxy-2′-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures. International Archives of Occupational and Environmental Health 80:1, pages 1-15.
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Chiung-Wen Hu, Chien-Jen Wang, Louis W Chang & Mu-Rong Chao. (2006) Clinical-Scale High-Throughput Analysis of Urinary 8-Oxo-7,8-Dihydro-2′-Deoxyguanosine by Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry with On-Line Solid-Phase Extraction. Clinical Chemistry 52:7, pages 1381-1388.
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J.M. Poul, S. Huet, T. Godard & P. Sanders. (2004) Lack of genotoxicity of potassium iodate in the alkaline comet assay and in the cytokinesis-block micronucleus test. Comparison to potassium bromate. Food and Chemical Toxicology 42:2, pages 203-209.
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P. Duez, G. Dehon, A. Kumps & J. Dubois. (2003) Statistics of the Comet assay: a key to discriminate between genotoxic effects. Mutagenesis 18:2, pages 159-166.
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Mónika Mórocz, János Kálmán, Anna Juhász, Ildikó Sinkó, Angela P McGlynn, C.Stephen Downes, Zoltán Janka & István Raskó. (2002) Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer’s disease11Abbreviations used: AD, Alzheimer’s disease; Endo III, Endonuclease III; 8OHA, 8-hydroxyadenine; 8OHG, 8-hydroxyguanine; 8OHdG, 8-hydroxy-2′-deoxyguanosine; Fapy-Ade, 4,6-diamino-5-formamidopyrimidine; Fapy-Gua, 2,6,-diamino-4-hydroxy-5-formamidopyrimidine; Fpg, formamidopyrimidine glycosilase; H2O2, hydrogen-peroxide; .OH, hydroxyl radical; PBS, phosphate-buffered saline; ROS, reactive oxygen species; RT, room temperature; SSB, single strand break. Neurobiology of Aging 23:1, pages 47-53.
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ZhongMao Guo, Hong Yang, Michelle L Hamilton, Holly VanRemmen & Arlan Richardson. (2001) Effects of age and food restriction on oxidative DNA damage and antioxidant enzyme activities in the mouse aorta. Mechanisms of Ageing and Development 122:15, pages 1771-1786.
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Rie Yoshida, Yasutaka Ogawa & Shinnosuke Takayanagi. (2001) In isolated DNA, formamidopyrimidine-DNA glycosylase-sensitive sites determined by electrophoresis correspond to the amount of 8-oxo-7,8-dihydro-2′-deoxyguanosine by HPLC-ECD. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 494:1-2, pages 55-61.
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Meeta Jaiswal, Nicholas F. LaRusso, Richard A. Shapiro, Timothy R. Billiar & Gregory J. Gores. (2001) Nitric oxide?mediated inhibition of DNA repair potentiates oxidative DNA damage in cholangiocytes. Gastroenterology 120:1, pages 190-199.
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Aqel Abu-Qare & Mohamed Abou-Donia. (2000) Increased 8-hydroxy-2′-deoxyguanosine, a biomarker of oxidative DNA damage in rat urine following a single dermal dose of DEET (N,N-diethyl-m-toluamide), and permethrin, alone and in combination. Toxicology Letters 117:3, pages 151-160.
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