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Journal of Receptors and Signal Transduction Volume 27, 2007 - Issue 4
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Research Article

Determination of Affinity and Activity of Ligands at the Human Neuropeptide Y Y4 Receptor by Flow Cytometry and Aequorin Luminescence

RALF ZIEMEK Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
,
ERICH SCHNEIDER Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
,
ANJA KRAUS Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
,
CHIARA CABRELE Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
,
ANNETTE G. BECK-SICKINGER Institut für Biochemie, Universität Leipzig, Leipzig, Germany
,
GÜNTHER BERNHARDT Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
&
ARMIN BUSCHAUER Institut für Pharmazie, Universität Regensburg, Regensburg, Germany
 show all
Pages 217-233 | Published online: 10 Oct 2008

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Nathalie Pop, Patrick Igel, Albert Brennauer, Chiara Cabrele, Günther Bernhardt, Roland Seifert & Armin Buschauer. (2011) Functional reconstitution of human neuropeptide Y (NPY) Y2 and Y4 receptors in Sf9 insect cells. Journal of Receptors and Signal Transduction 31:4, pages 271-285.
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Articles from other publishers (30)

Eva Plut, Jacqueline C. Calderón, Vesna Stanojlović, Albert O. Gattor, Carina Höring, Laura J. Humphrys, Adam Konieczny, Sabine Kerres, Mario Schubert, Max Keller, Chiara Cabrele, Timothy Clark & Oliver Reiser. (2023) Stereochemistry-Driven Interactions of α,γ-Peptide Ligands with the Neuropeptide Y Y 4 -Receptor . Journal of Medicinal Chemistry 66:14, pages 9642-9657.
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Inês C. F. Fonseca, Miguel Castelo-Branco, Cláudia Cavadas & Antero J. Abrunhosa. (2022) PET Imaging of the Neuropeptide Y System: A Systematic Review. Molecules 27:12, pages 3726.
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Christoph Müller, Jakob Gleixner, Maris-Johanna Tahk, Sergei Kopanchuk, Tõnis Laasfeld, Michael Weinhart, Dieter Schollmeyer, Martin U. Betschart, Steffen Lüdeke, Pierre Koch, Ago Rinken & Max Keller. (2022) Structure-Based Design of High-Affinity Fluorescent Probes for the Neuropeptide Y Y 1 Receptor . Journal of Medicinal Chemistry 65:6, pages 4832-4853.
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Adam Konieczny, Marcus Conrad, Fabian J. Ertl, Jakob Gleixner, Albert O. Gattor, Lukas Grätz, Maximilian F. Schmidt, Eduard Neu, Anselm H. C. Horn, David Wifling, Peter Gmeiner, Timothy Clark, Heinrich Sticht & Max Keller. (2021) N-Terminus to Arginine Side-Chain Cyclization of Linear Peptidic Neuropeptide Y Y 4 Receptor Ligands Results in Picomolar Binding Constants . Journal of Medicinal Chemistry 64:22, pages 16746-16769.
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Corinna Schüß, Oanh Vu, Mario Schubert, Yu Du, Nigam M. Mishra, Iain R. ToughJan Stichel, C. David Weaver, Kyle A. Emmitte, Helen M. CoxJens Meiler & Annette G. Beck-Sickinger. (2021) Highly Selective Y 4 Receptor Antagonist Binds in an Allosteric Binding Pocket . Journal of Medicinal Chemistry 64:5, pages 2801-2814.
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Adam Konieczny, Diana Braun, David Wifling, Günther Bernhardt & Max Keller. (2020) Oligopeptides as Neuropeptide Y Y 4 Receptor Ligands: Identification of a High-Affinity Tetrapeptide Agonist and a Hexapeptide Antagonist . Journal of Medicinal Chemistry 63:15, pages 8198-8215.
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D. Lachmann, A. Konieczny, M. Keller & B. König. (2019) Photochromic peptidic NPY Y 4 receptor ligands . Organic & Biomolecular Chemistry 17:9, pages 2467-2478.
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Stephen Quirk, Mandi M. Hopkins, Hailey Bureau, Ryan J. Lusk, Caley Allen, Rigoberto Hernandez & David L. Bain. (2018) Mutational Analysis of Neuropeptide Y Reveals Unusual Thermal Stability Linked to Higher-Order Self-Association. ACS Omega 3:2, pages 2141-2154.
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Kilian K. Kuhn, Timo Littmann, Stefanie Dukorn, Miho Tanaka, Max Keller, Takeaki Ozawa, Günther Bernhardt & Armin Buschauer. (2017) In Search of NPY Y 4 R Antagonists: Incorporation of Carbamoylated Arginine, Aza-Amino Acids, or d -Amino Acids into Oligopeptides Derived from the C-Termini of the Endogenous Agonists . ACS Omega 2:7, pages 3616-3631.
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X. Xu, B. Yu, W. Cai & Z. Huang. (2017) TCF1 deficiency ameliorates autoimmune lymphoproliferative syndrome (ALPS)-like phenotypes of lpr/lpr mice . Scandinavian Journal of Immunology 85:6, pages 406-416.
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Stefanie Dukorn, Timo Littmann, Max Keller, Kilian Kuhn, Chiara Cabrele, Paul Baumeister, Günther Bernhardt & Armin Buschauer. (2017) Fluorescence- and Radiolabeling of [Lys 4 ,Nle 17,30 ]hPP Yields Molecular Tools for the NPY Y 4 Receptor . Bioconjugate Chemistry 28:4, pages 1291-1304.
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Xiaoxie Xu, Jun Huang, Mei Zhao, Huanpeng Chen, Jinhua Mo, Xiaoqing Zhou, Qiao Su, Bolan Yu & Zhaofeng Huang. (2017) Stabilized β -Catenin Ameliorates ALPS-Like Symptoms of B6/ lpr Mice . Journal of Immunology Research 2017, pages 1-12.
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S. Lieb, T. Littmann, N. Plank, J. Felixberger, M. Tanaka, T. Sch?fer, S. Krief, S. Elz, K. Friedland, G. Bernhardt, J. Wegener, T. Ozawa & A. Buschauer. (2016) Label-free versus conventional cellular assays: Functional investigations on the human histamine H1 receptor. Pharmacological Research 114, pages 13-26.
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Kilian K. Kuhn, Thomas Ertl, Stefanie Dukorn, Max Keller, Günther Bernhardt, Oliver Reiser & Armin Buschauer. (2016) High Affinity Agonists of the Neuropeptide Y (NPY) Y 4 Receptor Derived from the C-Terminal Pentapeptide of Human Pancreatic Polypeptide (hPP): Synthesis, Stereochemical Discrimination, and Radiolabeling . Journal of Medicinal Chemistry 59:13, pages 6045-6058.
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Max Keller, Kilian K. Kuhn, Jürgen Einsiedel, Harald Hübner, Sabrina Biselli, Catherine Mollereau, David Wifling, Jaroslava Svobodová, Günther Bernhardt, Chiara Cabrele, Patrick M. L. Vanderheyden, Peter Gmeiner & Armin Buschauer. (2016) Mimicking of Arginine by Functionalized N ω -Carbamoylated Arginine As a New Broadly Applicable Approach to Labeled Bioactive Peptides: High Affinity Angiotensin, Neuropeptide Y, Neuropeptide FF, and Neurotensin Receptor Ligands As Examples . Journal of Medicinal Chemistry 59:5, pages 1925-1945.
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Stefanie Dukorn, Max Keller, Kilian Kuhn, Chiara Cabrele, G?nther Bernhardt & Armin Buschauer. (2016) Molecular tools for the NPY Y4 receptor: Fluorescence- and radiolabelled [Lys4,Nle17,30]hPP. Neuropeptides 55, pages 17.
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Sebastian Lieb. (2016) Dynamic mass redistribution as a label-free method for investigations on human NPY Y2 and Y4 receptors. Neuropeptides 55, pages 15-16.
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Max Keller, Stefan Weiss, Christoph Hutzler, Kilian K. Kuhn, Catherine Mollereau, Stefanie Dukorn, Lisa Schindler, Günther Bernhardt, Burkhard König & Armin Buschauer. (2015) N ω -Carbamoylation of the Argininamide Moiety: An Avenue to Insurmountable NPY Y 1 Receptor Antagonists and a Radiolabeled Selective High-Affinity Molecular Tool ([ 3 H]UR-MK299) with Extended Residence Time . Journal of Medicinal Chemistry 58:22, pages 8834-8849.
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Max Keller, Lisa Schindler, G?nther Bernhardt & Armin Buschauer. (2015) Toward Labeled Argininamide-Type NPY Y 1 Receptor Antagonists: Identification of a Favorable Propionylation Site in BIBO3304 . Archiv der Pharmazie 348:6, pages 390-398.
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Rajashri Sridharan, Jeffrey Zuber, Sara M. Connelly, Elizabeth Mathew & Mark E. Dumont. (2014) Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors. Biochimica et Biophysica Acta (BBA) - Biomembranes 1838:1, pages 15-33.
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Łukasz Berlicki, Melanie Kaske, Raquel Gutiérrez-Abad, Günther Bernhardt, Ona Illa, Rosa M. Ortuño, Chiara Cabrele, Armin Buschauer & Oliver Reiser. (2013) Replacement of Thr 32 and Gln 34 in the C -Terminal Neuropeptide Y Fragment 25–36 by cis -Cyclobutane and cis -Cyclopentane β-Amino Acids Shifts Selectivity toward the Y 4 Receptor . Journal of Medicinal Chemistry 56:21, pages 8422-8431.
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Max Keller, Melanie Kaske, Tobias Holzammer, Günther Bernhardt & Armin Buschauer. (2013) Dimeric argininamide-type neuropeptide Y receptor antagonists: Chiral discrimination between Y1 and Y4 receptors. Bioorganic & Medicinal Chemistry 21:21, pages 6303-6322.
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Nikola Pluym, Paul Baumeister, Max Keller, Günther Bernhardt & Armin Buschauer. (2013) [ 3 H]UR-PLN196: A Selective Nonpeptide Radioligand and Insurmountable Antagonist for the Neuropeptide Y Y 2  Receptor . ChemMedChem 8:4, pages 587-593.
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Nikola Pluym, Albert Brennauer, Max Keller, Ralf Ziemek, Nathalie Pop, G?nther Bernhardt & Armin Buschauer. (2011) Application of the Guanidine-Acylguanidine Bioisosteric Approach to Argininamide-Type NPY Y2 Receptor Antagonists. ChemMedChem 6:9, pages 1727-1738.
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Max Keller, G?nther Bernhardt & Armin Buschauer. (2011) [3H]UR-MK136: A Highly Potent and Selective Radioligand for Neuropeptide Y Y1 Receptors. ChemMedChem 6:9, pages 1566-1571.
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Max Keller, Daniela Erdmann, Nathalie Pop, Nikola Pluym, Shangjun Teng, Günther Bernhardt & Armin Buschauer. (2011) Red-fluorescent argininamide-type NPY Y1 receptor antagonists as pharmacological tools. Bioorganic & Medicinal Chemistry 19:9, pages 2859-2878.
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Max Keller, Shangjun Teng, Günther Bernhardt & Armin Buschauer. (2009) Bivalent Argininamide-Type Neuropeptide Y Y 1 Antagonists Do Not Support the Hypothesis of Receptor Dimerisation . ChemMedChem 4:10, pages 1733-1745.
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Cláudia Cavadas, Joana Salgado & Eric Grouzmann. 2009. Bioactive Peptides. Bioactive Peptides 43 66 .
Prasanta Ghorai, Anja Kraus, Max Keller, Carsten Götte, Patrick Igel, Erich Schneider, David Schnell, Günther Bernhardt, Stefan Dove, Manfred Zabel, Sigurd Elz, Roland Seifert & Armin Buschauer. (2008) Acylguanidines as Bioisosteres of Guanidines: N G -Acylated Imidazolylpropylguanidines, a New Class of Histamine H 2 Receptor Agonists . Journal of Medicinal Chemistry 51:22, pages 7193-7204.
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Erich Schneider, Max Keller, Albert Brennauer, Bianca K. Hoefelschweiger, Dietmar Gross, Otto S. Wolfbeis, Günther Bernhardt & Armin Buschauer. (2007) Synthesis and Characterization of the First Fluorescent Nonpeptide NPY Y1 Receptor Antagonist. ChemBioChem 8:16, pages 1981-1988.
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