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Original Articles

A Direct Preparative High Performance Liquid Chromatography Procedure for the Isolation of Major Triterpenoids and Their Quantitative Determination in Neem Oil

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Pages 3465-3471 | Received 01 Apr 1995, Accepted 13 Apr 1995, Published online: 23 Sep 2006

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Ramasamy Mohankumar, Kaliappan Ilango, Vichangal Pridiuldi Santhanakrishnan, Viswanathan Radhakrishnan & Srinivasan Narasimhan. (2010) Ethylenediamine: an effective reagent for deacetylation of natural products. Journal of Asian Natural Products Research 12:10, pages 851-858.
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Supradip Saha, Suresh Walia, Jitendra Kumar, BalrajS. Parmer & Dwarika Prasad. (2010) Synergistic/potentiation interaction between nematostatic constituents from Azadirachta indica, Madhuca indica and Sapindus mukorossi . Archives of Phytopathology and Plant Protection 43:4, pages 357-367.
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T.R. Govindachari, Geetha Gopalakrishnan, R. Malathi & N.D. Pradeep Singh. (1999) The 11α-H And 11β-H Epimers Of Azadirachtin H. Natural Product Letters 13:3, pages 157-162.
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T.R. Govindachari, G.N.Krishna Kumari & G. Suresh. (1997) Separation and Isolation of Limonoids from Khaya Senegalensis by Direct High Performance Liquid Chromatography. Journal of Liquid Chromatography & Related Technologies 20:19, pages 3189-3192.
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T.R. Govindachari, G. Gopalakrishnan & G. Suresh. (1996) Isolation of Various Azadirachtins from Neem Oil by Preparative High Performance Liquid Chromatography. Journal of Liquid Chromatography & Related Technologies 19:11, pages 1729-1733.
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Articles from other publishers (23)

Fatima Shirin, Anamika Rai & Neelu Singh. (2017) Variation in seed morphometric characters, oil content and azadirachtin content of seeds, in vitro shoot cultures and callus cultures among different populations of Azadirachta indica. Journal of Forestry Research 29:1, pages 121-127.
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V.P. Santhanakrishnan, R. Mohankumar & S. Narasimhan. (2016) Solid supported reagents for effecting selective transformation in natural products. Arabian Journal of Chemistry 9, pages S1260-S1264.
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Ompal Singh, Hamir Rathore & Leo Nollet. 2015. Biopesticides Handbook. Biopesticides Handbook 183 224 .
Paul Van der Meeren & Jan Vanderdeelen. 2012. Advances in Lipid Methodology. Advances in Lipid Methodology 83 118 .
Qin-Gang Tan & Xiao-Dong Luo. (2011) Meliaceous Limonoids: Chemistry and Biological Activities. Chemical Reviews 111:11, pages 7437-7522.
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Parvathi Chary. (2011) A comprehensive study on characterization of elite Neem chemotypes through mycofloral, tissue-cultural, ecomorphological and molecular analyses using azadirachtin-A as a biomarker. Physiology and Molecular Biology of Plants 17:1, pages 49-64.
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Yu-Qun Zhang, Jiao Xu, Zhong-Qiong Yin, Ren-Yong Jia, Yang Lu, Fan Yang, Yong-Hua Du, Ping Zou, Cheng Lv, Ting-Xiu Hu, Shu-Liang Liu, Gang Shu & Geng Yi. (2010) Isolation and identification of the antibacterial active compound from petroleum ether extract of neem oil. Fitoterapia 81:7, pages 747-750.
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M.A. Moslem & E.M. El-Kholie. (2009) Effect of Neem (Azardirachta indica A. Juss) Seeds and Leaves Extract on Some Plant Pathogenic Fungi. Pakistan Journal of Biological Sciences 12:14, pages 1045-1048.
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Bina Shaheen Siddiqui, Syed Kashif Ali, Syed Tariq Ali, Syed Naeem ul Hassan Naqvi & Rajput Muhammad Tariq. (2019) Variation of Major Limonoids in Azadirachta Indica Fruits at Different Ripening Stages and Toxicity against Aedes Aegypti . Natural Product Communications 4:4, pages 1934578X0900400.
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Júlio César T. Silva, Gulab N. Jham, Rosângela D’arc L. Oliveira & Leslie Brown. (2007) Purification of the seven tetranortriterpenoids in neem (Azadirachta indica) seed by counter-current chromatography sequentially followed by isocratic preparative reversed-phase high-performance liquid chromatography. Journal of Chromatography A 1151:1-2, pages 203-210.
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Geetha Gopalakrishnan, N.D Pradeep Singh, V Kasinath, M Siva Rama Krishnan, R Malathi & S.S Rajan. (2001) Microwave- and ultrasound-assisted oxidation of bio-active limonoids. Tetrahedron Letters 42:37, pages 6577-6579.
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Geetha Gopalakrishnan, N. Pradeep Singh & V. Kasinath. (2001) Photomediated Transformation of Salannin, a Tetranortriterpenoid from Azadirachta indica A. Juss. Molecules 6:6, pages 551-556.
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T. R. Govindachari, G. Suresh & S. D. Wesley. (2000) Insect antifeedant and growth regulating activities of neem seed oil - the role of major tetranortriterpenoids. Journal of Applied Entomology 124:7-8, pages 287-291.
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T.R Govindachari, G Suresh, Geetha Gopalakrishnan, S Masilamani & B Banumathi. (2000) Antifungal activity of some tetranortriterpenoids. Fitoterapia 71:3, pages 317-320.
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Andrew P Jarvis & E David Morgan. (2000) Analysis of small samples of limonoids of neem (Azadirachta indica) using solid phase extraction from tissue culture. Phytochemical Analysis 11:3, pages 184-189.
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T.R Govindachari, Geetha Gopalakrishnan & G Suresh. (1999) Triterpenoidal constituents of an aqueous extract from neem kernels. Fitoterapia 70:6, pages 558-560.
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Andrew P. Jarvis, E. David Morgan & Christine Edwards. (1999) Rapid separation of triterpenoids from Neem seed extracts. Phytochemical Analysis 10:1, pages 39-43.
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T. R. Govindachari, G. Suresh, Geetha Gopalakrishnan, Balaganesan Banumathy & S. Masilamani. (1998) Identification of antifungal compounds from the seed oil ofAzadirachta Indica. Phytoparasitica 26:2, pages 109-116.
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V. Kabaleeswaran, S. S. Rajan, Geetha Gopalakrishnan, G. Suresh & T. R. Govindachari. (1997) Crystal structure of nimbin. Journal of Chemical Crystallography 27:12, pages 731-733.
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T.R. Govindachari & Geetha Gopalakrishnan. (1997) 13,14-desepoxyazadirachtin-A, a tetranortriterpenoid from Azadirachta indica. Phytochemistry 45:2, pages 397-399.
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Shaun Johnson & E.David Morgan. (1997) Comparison of chromatographic systems for triterpenoids from Neem (Azadirachta indica) seeds. Journal of Chromatography A 761:1-2, pages 53-63.
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T. R. Govindachari, N. S. Narasimhan, G. Suresh, P. D. Partho & Geetha Gopalakrishnan. (1996) Insect antifeedant and growth-regulating activities of Salannin and other c-seco limonoids from neem oil in relation to Azadirachtin. Journal of Chemical Ecology 22:8, pages 1453-1461.
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John G. Dorsey, William T. Cooper, Barbara A. Siles, Joe P. Foley & Howard G. Barth. (1996) Liquid Chromatography:  Theory and Methodology. Analytical Chemistry 68:12, pages 515-568.
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