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Genetic toxicity assessment using liver cell models: past, present, and future

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Ezeidy Denisse Gallardo-Valle, Dayanne Carbajal-Nogueda, Ma. Elena Moreno-Godínez, Eugenia Flores-Alfaro, Isela Parra-Rojas, Gerardo Huerta-Beristain, Teresa Domínguez-Reyes & Marco Antonio Ramírez-Vargas. (2023) Evaluation of the cytotoxicity and genotoxicity of glufosinate-ammonium at technical and commercial grades in HepG2 cells. Journal of Environmental Science and Health, Part B 58:8, pages 577-582.
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Ana Paula Oliveira de Quadros, Isabel Bragança Baraldi, Marcel Petreanu, Rivaldo Niero, Mario Sergio Mantovani, Isabel O’Neill De Mascarenhas Gaivão & Edson Luis Maistro. (2023) Cytogenotoxic evaluations of leaves and stems extracts of Rubus rosifolius in primary metabolically noncompetent cells. Journal of Toxicology and Environmental Health, Part A 86:11, pages 361-371.
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Gessyca Costa, Andréia Fernandes, Thaís Santos, Lara Brito, Laís Rodrigues, Marize Valadares, Israel Felzenszwalb, Elisa Ferraz, Daniela Morais Leme & Gisele Oliveira. (2022) In vitro and in vivo cytotoxicity assessment of glyphosate and imazethapyr-based herbicides and their association. Journal of Toxicology and Environmental Health, Part A 85:12, pages 481-493.
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Xilin Li, Yuxi Li, Kylie G. Ning, Si Chen, Lei Guo, Jessica A. Bonzo & Nan Mei. (2022) The expression of Phase II drug-metabolizing enzymes in human B-lymphoblastoid TK6 cells. Journal of Environmental Science and Health, Part C 40:1, pages 106-118.
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Saulo Duarte Ozelin, Juliana Marques Senedese, Jacqueline Morais Alves, Carla Carolina Munari, Juliana De Carvalho Da Costa, Flávia Aparecida Resende, Débora Leite Campos, Ildercílio Mota De Souza Lima, Augusto Faria Andrade, Eliana Aparecida Varanda, Jairo Kenupp Bastos & Denise Crispim Tavares. (2021) Preventive activity of Copaifera langsdorffii Desf. leaves extract and its major compounds, afzelin and quercitrin, on DNA damage in in vitro and in vivo models. Journal of Toxicology and Environmental Health, Part A 84:14, pages 569-581.
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Brian Ogushi Silva, Juliana Botinhon Orlando, Camila Lehnhardt Pires, Clélia Akiko Hiruma-Lima, Isabel de Mascarenhas Gaivão, Fábio Ferreira Perazzo & Edson Luis Maistro. (2021) Genotoxicity induced by nerol, an essential oil present in citric plants using human peripheral blood mononuclear cells (PBMC) and HepG2/C3A cells as a model. Journal of Toxicology and Environmental Health, Part A 84:12, pages 518-528.
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Xiaoqing Guo, Ji-Eun Seo, Dayton Petibone, Volodymyr Tryndyak, Un Jung Lee, Tong Zhou, Timothy W. Robison & Nan Mei. (2020) Performance of HepaRG and HepG2 cells in the high-throughput micronucleus assay for in vitro genotoxicity assessment. Journal of Toxicology and Environmental Health, Part A 83:21-22, pages 702-717.
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Kaat Leroy, Alanah Pieters, Andrés Tabernilla, Axelle Cooreman, Raf Van Campenhout, Bruno Cogliati & Mathieu Vinken. (2020) Targeting gap junctional intercellular communication by hepatocarcinogenic compounds. Journal of Toxicology and Environmental Health, Part B 23:6, pages 255-275.
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Ana Paula Oliveira De Quadros, Laíza Moura Almeida, Marcel Petreanu, Rivaldo Niero, Paulo Cesar Pires Rosa, Alexandra Christinie Helena Frankland Sawaya, Mario Sergio Mantovani, Isabel O’Neill De Mascarenhas Gaivão & Edson Luis Maistro. (2020) Risk assessment via genotoxicity, metabolism, apoptosis, and cell growth effects in a HepG2/C3A cell line upon treatment with Rubus rosifolius (Rosaceae) leaves extract. Journal of Toxicology and Environmental Health, Part A 83:13-14, pages 495-508.
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Ali Kermanizadeh, Leagh G Powell & Vicki Stone. (2020) A review of hepatic nanotoxicology – summation of recent findings and considerations for the next generation of study designs. Journal of Toxicology and Environmental Health, Part B 23:4, pages 137-176.
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Articles from other publishers (24)

Ji-Eun Seo, Joshua Z. Yu, Hannah Xu, Xilin Li, Aisar H. Atrakchi, Timothy J. McGovern, Karen L. Davis Bruno, Nan Mei, Robert H. Heflich & Xiaoqing Guo. (2023) Genotoxicity assessment of eight nitrosamines using 2D and 3D HepaRG cell models. Archives of Toxicology 97:10, pages 2785-2798.
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Yanjie Dong, Da Cai, Chao Liu, Shancang Zhao & Lei Wang. (2023) Combined cytotoxicity of phthalate esters on HepG2 cells: A comprehensive analysis of transcriptomics and metabolomics. Food and Chemical Toxicology, pages 114034.
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Edvannia dos Santos Silva, Maria João Matos & Edson Luis Maistro. (2023) Evaluation of in vitro cytotoxic and genotoxic effects of the 3‐(3,4‐dihydroxyphenyl)‐8‐hydroxycoumarin. Journal of Applied Toxicology.
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Lingfei Han, Yingjiu Zhou, Zheng Tan, Hongtan Zhu, Yexin Hu, Xiao Ma, Feng Zheng, Feng Feng, Chen Wang & Wenyuan Liu. (2023) Confined Target-Triggered Hot Spots for In Situ SERS Analysis of Intranuclear Genotoxic Markers. Analytical Chemistry 95:15, pages 6312-6322.
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Ji-Eun Seo, Xilin Li, Yuan Le, Nan Mei, Tong Zhou & Xiaoqing Guo. (2023) High-throughput micronucleus assay using three-dimensional HepaRG spheroids for in vitro genotoxicity testing. Archives of Toxicology 97:4, pages 1163-1175.
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Francesca Caloni, Isabella De Angelis & Thomas Hartung. (2022) Replacement of animal testing by integrated approaches to testing and assessment (IATA): a call for in vivitrosi. Archives of Toxicology 96:7, pages 1935-1950.
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Katrin Kreuzer, Heike Sprenger & Albert Braeuning. (2022) Comparative Analysis of Transcriptional Responses to Genotoxic and Non-Genotoxic Agents in the Blood Cell Model TK6 and the Liver Model HepaRG. International Journal of Molecular Sciences 23:7, pages 3420.
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Nupur Vasdev, Mrudul Deshpande, Pratik Katare, Vaishali Makwana, Suryanarayana Polaka, Muktika Tekade, Pinaki Sengupta, Mukesh Chandra Sharma, Dinesh Kumar Mishra & Rakesh Kumar Tekade. 2022. Pharmacokinetics and Toxicokinetic Considerations. Pharmacokinetics and Toxicokinetic Considerations 175 219 .
Sophie Rose, Marie Cuvellier, Frédéric Ezan, Jennifer Carteret, Arnaud Bruyère, Vincent Legagneux, Fabrice Nesslany, Georges Baffet & Sophie Langouët. (2021) DMSO-free highly differentiated HepaRG spheroids for chronic toxicity, liver functions and genotoxicity studies. Archives of Toxicology 96:1, pages 243-258.
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Haokang Zhang & Leo Q. Wan. (2021) Cell Chirality as a Novel Measure for Cytotoxicity. Advanced Biology 6:1.
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Guilherme Ribeiro Romualdo, Kaat Leroy, Cícero Júlio Silva Costa, Gabriel Bacil Prata, Bart Vanderborght, Tereza Cristina da Silva, Luís Fernando Barbisan, Wellington Andraus, Lindsey Devisscher, Niels Olsen Saraiva Câmara, Mathieu Vinken & Bruno Cogliati. (2021) In Vivo and In Vitro Models of Hepatocellular Carcinoma: Current Strategies for Translational Modeling. Cancers 13:21, pages 5583.
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Kaat Leroy, Alanah Pieters, Axelle Cooreman, Raf Van Campenhout, Bruno Cogliati & Mathieu Vinken. (2021) Connexin-Based Channel Activity Is Not Specifically Altered by Hepatocarcinogenic Chemicals. International Journal of Molecular Sciences 22:21, pages 11724.
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Ji-Eun Seo, Kelly Davis, Pritpal Malhi, Xiaobo He, Matthew Bryant, John Talpos, Susan Burks, Nan Mei & Xiaoqing Guo. (2021) Genotoxicity evaluation using primary hepatocytes isolated from rhesus macaque (Macaca mulatta). Toxicology 462, pages 152936.
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Nicholas J. Coltman, Brandon A. Coke, Kyriaki Chatzi, Emma L. Shepherd, Patricia F. Lalor, Timothy Schulz-Utermoehl & Nikolas J. Hodges. (2021) Application of HepG2/C3A liver spheroids as a model system for genotoxicity studies. Toxicology Letters 345, pages 34-45.
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Yiying Wang, Roberta A. Mittelstaedt, Rebecca Wynne, Ying Chen, Xuefei Cao, Levan Muskhelishvili, Kelly Davis, Timothy W. Robison, Wei Sun, Elizabeth K. Schmidt, Thomas H. Smith, Zachary K. Norgaard, Charles C. Valentine, Jeffry Yaplee, Lindsey N. Williams, Jesse J. Salk & Robert H. Heflich. (2021) Genetic toxicity testing using human in vitro organotypic airway cultures: Assessing DNA damage with the CometChip and mutagenesis by Duplex Sequencing . Environmental and Molecular Mutagenesis 62:5, pages 306-318.
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Tasnia Sharin, Helina Gyasi, Stephanie P. Jones, Doug Crump & Jason M. O'Brien. (2021) Concentration‐ and time‐dependent induction of Cyp1a and DNA damage response by benzo(a)pyrene in LMH three‐dimensional spheroids . Environmental and Molecular Mutagenesis 62:5, pages 319-327.
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Nathalia de Assis Aguilar Duarte, Lindiane Eloisa de Lima, Flora Troina Maraslis, Michael Kundi, Emilene Arusievicz Nunes & Gustavo Rafael Mazzaron Barcelos. (2021) Acute Toxicity and DNA Instability Induced by Exposure to Low Doses of Triclosan and Phthalate DEHP, and Their Combinations, in vitro. Frontiers in Genetics 12.
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Nan Mei, Xilin Li, Si Chen, Lei Guo & Xiaoqing Guo. 2021. Nutraceuticals. Nutraceuticals 1199 1211 .
Miroslav Mišík, Franziska Ferk, Heidemarie Schaar, Masami Yamada, Walter Jaeger, Siegfried Knasmueller & Norbert Kreuzinger. (2020) Genotoxic activities of wastewater after ozonation and activated carbon filtration: Different effects in liver-derived cells and bacterial indicators. Water Research 186, pages 116328.
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Xilin Li, Xiaobo He, Si Chen, Xiaoqing Guo, Matthew S. Bryant, Lei Guo, Mugimane G. Manjanatha, Tong Zhou, Kristine L. Witt & Nan Mei. (2020) Evaluation of pyrrolizidine alkaloid-induced genotoxicity using metabolically competent TK6 cell lines. Food and Chemical Toxicology 145, pages 111662.
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Thilona Arumugam, Terisha Ghazi & Anil Chuturgoon. (2020) Fumonisin B1 Epigenetically Regulates PTEN Expression and Modulates DNA Damage Checkpoint Regulation in HepG2 Liver Cells. Toxins 12:10, pages 625.
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Vaishali M. Patil, Shipra Singhal & Neeraj Masand. (2020) A systematic review on use of aminoquinolines for the therapeutic management of COVID-19: Efficacy, safety and clinical trials. Life Sciences 254, pages 117775.
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Xilin Li, Si Chen, Xiaoqing Guo, Qiangen Wu, Ji-Eun Seo, Lei Guo, Mugimane G Manjanatha, Tong Zhou, Kristine L Witt & Nan Mei. (2020) Development and Application of TK6-derived Cells Expressing Human Cytochrome P450s for Genotoxicity Testing. Toxicological Sciences 175:2, pages 251-265.
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Ji-Eun Seo, Qiangen Wu, Matthew Bryant, Lijun Ren, Qiang Shi, Timothy W. Robison, Nan Mei, Mugimane G. Manjanatha & Xiaoqing Guo. (2020) Performance of high-throughput CometChip assay using primary human hepatocytes: a comparison of DNA damage responses with in vitro human hepatoma cell lines. Archives of Toxicology 94:6, pages 2207-2224.
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