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Biotechnology & Biotechnological Equipment Volume 29, 2015 - Issue 1
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Article; Medical Biotechnology

Complementary annealing mediated by exonuclease: a method for seamless cloning and conditioning site-directed mutagenesis

Shuhui SunInstitute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, #16 Xuelin Street, Hangzhou, Zhejiang310036, ChinaView further author information
,
Hao HuangInstitute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, #16 Xuelin Street, Hangzhou, Zhejiang310036, ChinaView further author information
,
Yingchuan Billy QiInstitute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, #16 Xuelin Street, Hangzhou, Zhejiang310036, ChinaView further author information
,
Mengsheng QiuInstitute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, #16 Xuelin Street, Hangzhou, Zhejiang310036, China;Department of Anatomical Sciences and Neurobiology, School of Medicine, University of Louisville, Louisville, Kentucky, KY40292, USAView further author information
&
Zhong-Min DaiInstitute of Developmental and Regenerative Biology, College of Life and Environmental Sciences, Hangzhou Normal University, #16 Xuelin Street, Hangzhou, Zhejiang310036, ChinaCorrespondence[email protected]
View further author information
Pages 105-110 | Received 16 Jul 2014, Accepted 18 Sep 2014, Published online: 10 Dec 2014

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Wei Guo, Shuhui Sun, Binghua Xie, Xiao-Jing Zhu & Zhong-Min Dai. (2016) T4 DNA polymerase improves the efficiency of multiple site-directed mutagenesis. Biotechnology & Biotechnological Equipment 30:4, pages 721-725.
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Articles from other publishers (6)

Wei Guo, Binhua Xie, Min Jiang, Xiao-Jing Zhu, Mengsheng Qiu & Zhong-Min Dai. (2019) An improved overlap extension PCR for simultaneous multiple sites large fragments insertion, deletion and substitution. Scientific Reports 9:1.
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Shuhui Sun, Xiao‐Jing Zhu, Hao Huang, Wei Guo, Tao Tang, Binghua Xie, Xiaofeng Xu, Zunyi Zhang, Ying Shen, Zhong‐Min Dai & Mengsheng Qiu. (2019) WNT signaling represses astrogliogenesis via Ngn2‐dependent direct suppression of astrocyte gene expression. Glia 67:7, pages 1333-1343.
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Shuhui Sun, Wei Guo, Jin-Shu Yang, Mengsheng Qiu, Xiao-Jing Zhu & Zhong-Min Dai. (2018) TT(N)mGCCTC inhibits archaeal family B DNA polymerases. Scientific Reports 8:1.
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Kazi Rahman, Msano Mandalasi, Peng Zhao, M. Osman Sheikh, Rahil Taujale, Hyun W. Kim, Hanke van der Wel, Khushi Matta, Natarajan Kannan, John N. Glushka, Lance Wells & Christopher M. West. (2017) Characterization of a cytoplasmic glucosyltransferase that extends the core trisaccharide of the Toxoplasma Skp1 E3 ubiquitin ligase subunit. Journal of Biological Chemistry 292:45, pages 18644-18659.
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Kang Lan Tee & Tuck Seng Wong. 2017. Directed Enzyme Evolution: Advances and Applications. Directed Enzyme Evolution: Advances and Applications 201 227 .
Jian-zhong Xu & Wei-guo Zhang. (2016) Strategies used for genetically modifying bacterial genome: site-directed mutagenesis, gene inactivation, and gene over-expression遗传改造细菌基因组的策略:基因定点突变、基因失活和基因过表达. Journal of Zhejiang University-SCIENCE B 17:2, pages 83-99.
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