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Biotechnology & Biotechnological Equipment Volume 32, 2018 - Issue 1
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Article: Agriculture and Environmental Biotechnology

A statistical approach for optimizing the protocol for overexpressing lipase KV1 in Escherichia coli: purification and characterization

Kalaivani BatumalaieDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, MalaysiaORCID Iconhttp://orcid.org/0000-0002-9982-6587View further author information
ORCID Icon,
Elham KhaliliDepartment of Biotechnology and Medical Engineering, Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, Johor, MalaysiaView further author information
,
Naji Arafat MahatDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, MalaysiaView further author information
,
Fahrul Zaman HuyopDepartment of Biotechnology and Medical Engineering, Faculty of Bioscience and Medical Engineering, Universiti Teknologi Malaysia, Johor, MalaysiaORCID Iconhttp://orcid.org/0000-0003-3978-4087View further author information
ORCID Icon &
Roswanira Abdul WahabDepartment of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Johor, MalaysiaCorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0002-9982-6587View further author information
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Pages 69-87 | Received 02 Jul 2017, Accepted 17 Nov 2017, Published online: 24 Nov 2017

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Prem Chandra & Devendra Pratap Singh. (2023) Sources, purification, immobilization and industrial applications of microbial lipases: An overview. Critical Reviews in Food Science and Nutrition 63:24, pages 6653-6686.
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Nurul Fatin Syamimi Khairul Anuar, Roswanira Abdul Wahab, Fahrul Huyop, Syazwani Itri Amran, Azzmer Azzar Abdul Hamid, Khairul Bariyyah Abd Halim & Mohammad Hakim Mohammad Hood. (2021) Molecular docking and molecular dynamics simulations of a mutant Acinetobacter haemolyticus alkaline-stable lipase against tributyrin. Journal of Biomolecular Structure and Dynamics 39:6, pages 2079-2091.
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Nurul Fatin Syamimi Khairul Anuar, Roswanira Abdul Wahab, Fahrul Huyop, Khairul Bariyyah Abd Halim & Azzmer Azzar Abdul Hamid. (2020) In silico mutation on a mutant lipase from Acinetobacter haemolyticus towards enhancing alkaline stability. Journal of Biomolecular Structure and Dynamics 38:15, pages 4493-4507.
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Priyashini Dhaver, Tariro Sithole, Brett Pletschke, Bruce Sithole & Roshini Govinden. (2023) Enhanced production of a recombinant xylanase (XT6): optimization of production and purification, and scaled-up batch fermentation in a stirred tank bioreactor. Scientific Reports 13:1.
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Riyadh Benyettou, Salah Amroune, Mohamed Slamani, Khalissa Saada, Hassan Fouad, Mohammad Jawaid & S. Sikdar. (2023) Modelling and optimization of the absorption rate of date palm fiber reinforced composite using response surface methodology. Alexandria Engineering Journal 79, pages 545-555.
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Yangyang Cai, Shuqi Xing, Qifeng Zhang, Ruonan Zhu, Kai Cheng, Cuiqin Li, Xuefeng Zeng & Laping He. (2021) Expression, purification, properties, and substrate specificity analysis of Aspergillus niger GZUF36 lipase in Escherichia coli. Process Biochemistry 111, pages 118-127.
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Fabiano Jares Contesini, Marcelo Gomes Davanço, Gustavo Pagotto Borin, Katherina Garcia Vanegas, João Pedro Gonçalves Cirino, Ricardo Rodrigues de Melo, Uffe Hasbro Mortensen, Kristiina Hildén, Daniel Rossi Campos & Patricia de Oliveira Carvalho. (2020) Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry. Catalysts 10:9, pages 1032.
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Shuqi Xing, Ruonan Zhu, Cuiqin Li, Laping He, Xuefeng Zeng & Qing Zhang. (2020) Gene cloning, expression, purification and characterization of a sn-1,3 extracellular lipase from Aspergillus niger GZUF36. Journal of Food Science and Technology 57:7, pages 2669-2680.
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Christos Papaneophytou. (2019) Design of Experiments As a Tool for Optimization in Recombinant Protein Biotechnology: From Constructs to Crystals. Molecular Biotechnology 61:12, pages 873-891.
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Albert Uhoraningoga, Gemma Kinsella, Gary Henehan & Barry Ryan. (2018) The Goldilocks Approach: A Review of Employing Design of Experiments in Prokaryotic Recombinant Protein Production. Bioengineering 5:4, pages 89.
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Kalaivani Batumalaie, Elham Khalili, Naji Arafat Mahat, Fahrul Huyop & Roswanira Abdul Wahab. (2018) Biophysical characterization of a recombinant lipase KV1 from Acinetobacter haemolyticus in relation to pH and temperature. Biochimie 152, pages 198-210.
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