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Journal of Immunoassay and Immunochemistry Volume 29, 2007 - Issue 1
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Original Articles

Detection of Salmonella Typhimurium in Raw Meats using In‐House Prepared Monoclonal Antibody Coated Magnetic Beads and PCR Assay of the fimA Gene

Ângela N. Moreira Centro de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil
,
Fabricio R. Conceição Centro de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil
,
Rita de Cássia S. Conceição Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas, RS, Brazil
,
Roberta J. Ramos Centro de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil
,
José B. Carvalhal Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil
,
Odir A. Dellagostin Centro de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil
&
José A. G. Aleixo Centro de Biotecnologia, Universidade Federal de Pelotas, Pelotas, RS, Brazil; Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, BrazilCorrespondence[email protected]
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Pages 58-69 | Received 20 Apr 2007, Accepted 05 Jul 2007, Published online: 31 Dec 2007

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Xihong Zhao, Caijiao Wei, Junliang Zhong & Shiwei Jin. (2016) Research advance in rapid detection of foodborne Staphylococcus aureus. Biotechnology & Biotechnological Equipment 30:5, pages 827-833.
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Articles from other publishers (19)

Kåre Mølbak, Martyn Kirk, John Elmerdahl Olsen & John Besser. 2024. Encyclopedia of Food Safety. Encyclopedia of Food Safety 252 275 .
Lijun Hu, Guojie Cao, Eric W. Brown, Marc W. Allard, Li M. Ma & Guodong Zhang. (2021) Whole genome sequencing and protein structure analyses of target genes for the detection of Salmonella. Scientific Reports 11:1.
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Jiashun Wang, Yi Li, Jia Chen, Deping Hua, Yi Li, Hui Deng, Ying Li, Zhixuan Liang & Jinhai Huang. (2018) Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene. Brazilian Journal of Microbiology 49:2, pages 320-328.
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Spiros Paramithiotis, Agni Hadjilouka & Eleftherios H. Drosinos. 2018. Molecular Techniques in Food Biology. Molecular Techniques in Food Biology 215 237 .
Lijun Wang, Ronghui Wang, Hong Wang, Michael Slavik, Hua Wei & Yanbin Li. (2017) An aptamer-based PCR method coupled with magnetic immunoseparation for sensitive detection of Salmonella Typhimurium in ground turkey. Analytical Biochemistry 533, pages 34-40.
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Wai Hoe Chin, Yi Sun, Jonas Høgberg, Than Linh Quyen, Pia Engelsmann, Anders Wolff & Dang Duong Bang. (2017) Direct PCR – A rapid method for multiplexed detection of different serotypes of Salmonella in enriched pork meat samples. Molecular and Cellular Probes 32, pages 24-32.
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Wei Wu, Shiming Zhao, Yiping Mao, Zhiyuan Fang, Xuewen Lu & Lingwen Zeng. (2015) A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplification. Analytica Chimica Acta 861, pages 62-68.
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Xiaojuan Yang, Haigang Li, Qingping Wu, Jumei Zhang & Ling Chen. (2015) Comparison of Direct Culture, Immunomagnetic Separation/culture, and Multiplex PCR Methods for Detection of <i>Salmonella</i> in Food. Food Science and Technology Research 21:5, pages 671-675.
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Jun Xu, Junying Hu, Lingzi Liu, Li Li, Xu Wang, Huiyuan Zhang, Wei Jiang, Jiesheng Tian, Ying Li & Jilun Li. (2014) Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes. Frontiers in Microbiology 5.
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Padmavathy Bakthavathsalam, Vinoth Kumar Rajendran, Uttara Saran, Suvro Chatterjee & Baquir Mohammed Jaffar Ali. (2013) Immunomagnetic nanoparticle based quantitative PCR for rapid detection of Salmonella. Microchimica Acta 180:13-14, pages 1241-1248.
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Sanna Taskila, Mika Tuomola & Heikki Ojamo. (2012) Enrichment cultivation in detection of food-borne Salmonella. Food Control 26:2, pages 369-377.
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Jennifer RonholmZhiyi ZhangXundong CaoMin Lin. (2011) Monoclonal Antibodies to Lipopolysaccharide Antigens of Salmonella enterica serotype Typhimurium DT104 . Hybridoma 30:1, pages 43-52.
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K Z Brainina, A N Kozitsina & Y A Glazyrina. (2010) Hybrid Electrochemical/Magnetic Assay for Salmonella Typhimurium Detection. IEEE Sensors Journal 10:11, pages 1699-1704.
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Florian Mertes, Katja Biens, Hans Lehrach, Martin Wagner & Andreas Dahl. (2010) High-throughput Universal Probe Salmonella Serotyping (UPSS) by nanoPCR. Journal of Microbiological Methods 83:2, pages 217-223.
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Aihua Li, Huiyuan Zhang, Xin Zhang, Qi Wang, Jiesheng Tian, Ying Li & Jilun Li. (2010) Rapid separation and immunoassay for low levels of Salmonella in foods using magnetosome–antibody complex and real‐time fluorescence quantitative PCR . Journal of Separation Science 33:21, pages 3437-3443.
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Carlos G. Leon-Velarde, Leila Zosherafatein & Joseph A. Odumeru. (2009) Application of an automated immunomagnetic separation–enzyme immunoassay for the detection of Salmonella enterica subspecies enterica from poultry environmental swabs. Journal of Microbiological Methods 79:1, pages 13-17.
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Birce Mercanoglu Taban & Sait A. Aytac. (2009) Application of magnetic immuno-polymerase chain reaction assay for detection of Salmonella spp. in chicken meats. European Food Research and Technology 229:4, pages 623-628.
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Edel O'Regan, Evonne McCabe, Catherine Burgess, Sheila McGuinness, Thomas Barry, Geraldine Duffy, Paul Whyte & Séamus Fanning. (2008) Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. BMC Microbiology 8:1.
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Cláudia Pinho Hartleben FernandesFabiana Kömmling SeixasMariana Loner CoutinhoFlávia Aleixo VasconcellosÂngela Nunes MoreiraFabricio Rochedo ConceiçãoOdir Antônio DellagostinJosé Antonio Guimarães Aleixo. (2008) An Immunomagnetic Separation-PCR Method for Detection of Pathogenic Leptospira in Biological Fluids . Hybridoma 27:5, pages 381-386.
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