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RNA Biology Volume 17, 2020 - Issue 10
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Research paper

Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA

Polina Selkovaa Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, RussiaView further author information
,
Aleksandra Vasilevaa Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, RussiaView further author information
,
Georgii Pobegalovb Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaORCID Iconhttps://orcid.org/0000-0003-0836-0732View further author information
ORCID Icon,
Olga Musharovac Skolkovo Institute of Science and Technology, Center of Life Sciences, Moscow, Russia;d Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, RussiaORCID Iconhttps://orcid.org/0000-0003-2496-0420View further author information
ORCID Icon,
Anatolii Arsenieva Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, RussiaView further author information
,
Maksim Kazalovb Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaView further author information
,
Tatyana Zyubkob Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaView further author information
,
Nataliia Shcheglovab Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaView further author information
,
Tatyana Artamonovab Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaView further author information
,
Mikhail Khodorkovskiib Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, RussiaView further author information
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Konstantin Severinova Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia;d Institute of Molecular Genetics, Russian Academy of Sciences, Moscow, RussiaCorrespondence[email protected] [email protected]
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Iana Fedorovac Skolkovo Institute of Science and Technology, Center of Life Sciences, Moscow, RussiaORCID Iconhttps://orcid.org/0000-0001-6144-173XView further author information
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Pages 1472-1479 | Received 08 Feb 2020, Accepted 14 May 2020, Published online: 21 Jun 2020

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A. Vasileva, P. Selkova, A. Arseniev, M. Abramova, N. Shcheglova, O. Musharova, I. Mizgirev, T. Artamonova, M. Khodorkovskii, K. Severinov & I. Fedorova. (2023) Characterization of CoCas9 nuclease from Capnocytophaga ochracea. RNA Biology 20:1, pages 750-759.
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David A. Armstrong, Taylor R. Hudson, Christine A. Hodge, Thomas H. Hampton, Alexandra L. Howell & Matthew S. Hayden. (2023) PlmCas12e (CasX2) cleavage of CCR5: impact of guide RNA spacer length and PAM sequence on cleavage activity. RNA Biology 20:1, pages 296-305.
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Articles from other publishers (5)

A. A. Vasileva, S. A. Aliukas, P. A. Selkova, A. N. Arseniev, V. E. Chernova, O. S. Musharova, E. I. Klimuk, M. A. Khodorkovskii & K. V. Severinov. (2023) Type II CRISPR–Cas System Nucleases: A Pipeline for Prediction and In Vitro Characterization. Molecular Biology 57:3, pages 550-562.
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Guangqing Liu, Huiying Wang, Baisong Tong, Yali Cui, Sibylle C. Vonesch, Huina Dong & Dawei Zhang. (2023) An Efficient CRISPR/Cas12e System for Genome Editing in Sinorhizobium meliloti . ACS Synthetic Biology 12:3, pages 898-903.
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Chao Dong, Yue Xi, Viswanathan Satheesh & Mingguang Lei. (2023) Advances in CRISPR/Cas technologies and their application in plants. Tropical Plants 2:1, pages 1-10.
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Oddur Vilhelmsson, M. Audur Sigurbjornsdottir, Gudny Vala Thorsteinsdottir, Martina Cascone, Davide Corso, Luca Tonietti, Flavia Migliaccio, Nunzia Nappi, Annarita Ricciardelli, Matteo Selci, Francesco Montemagno, Bernardo Barosa, Deborah Bastoni, Alessia Bastianoni, Angelina Cordone & Donato Giovannelli. 2023. Thermophilic Anaerobes. Thermophilic Anaerobes 21 90 .
Oscar E Torres Montaguth, Stephen J Cross, Kincaid W A Ingram, Laura Lee, Fiona M Diffin & Mark D Szczelkun. (2021) ENDO-Pore: high-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing. Nucleic Acids Research 49:20, pages e118-e118.
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