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Bioengineered Volume 7, 2016 - Issue 3
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Research Paper

Enhanced recombinant factor VII expression in Chinese hamster ovary cells by optimizing signal peptides and fed-batch medium

Lin PengThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China
,
Xiao YuLaboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China
,
Chengyuan LiLaboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China
,
Yanfei CaiLaboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China
,
Yun ChenLaboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, China
,
Yang HeJiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Suzhou, China
,
Jianfeng YangCyrus Tang Hematology Center and Ministry of Education Engineering Center of Hematological Disease, Soochow University, Suzhou, China
,
Jian JinLaboratory of Molecular Pharmacology, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, ChinaCorrespondence[email protected] [email protected]
&
Huazhong LiThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, ChinaCorrespondence[email protected] [email protected]
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Pages 189-197 | Received 23 Mar 2016, Accepted 06 Apr 2016, Published online: 01 Jun 2016

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Saumel Pérez-Rodriguez, María de Jesús Ramírez-Lira, Mauricio A. Trujillo-Roldán & Norma A. Valdez-Cruz. (2020) Nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of Chinese hamster ovary cells. Bioengineered 11:1, pages 463-471.
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Lin Peng, Wendian Xiong, Yanfei Cai, Yun Chen, Yang He, Jianfeng Yang, Jian Jin & Huazhong Li. (2017) A simple, rapid method for evaluation of transfection efficiency based on fluorescent dye. Bioengineered 8:3, pages 225-231.
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Hao Song, Jingxin Jiang, Xuedong Wang & Jianguo Zhang. (2017) High purity recombinant human growth hormone (rhGH) expression in Escherichia coli under phoA promoter. Bioengineered 8:2, pages 147-153.
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Bhagyashree Bachhav, Jacopo de Rossi, Carlos D. Llanos & Laura Segatori. (2023) Cell factory engineering: Challenges and opportunities for synthetic biology applications. Biotechnology and Bioengineering 120:9, pages 2441-2459.
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Yanmin Zhang, Daoyuan Na, Weijian Zhang, Xuping Liu, Shiwei Miao, Wen-Song Tan & Liang Zhao. (2023) Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine 41:9, pages 1573-1583.
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Kadri Kangro, Elien Roose, Charlotte Dekimpe, Aline Vandenbulcke, Nuno A.G. Graça, Jan Voorberg, Mart Ustav, Andres Männik & Karen Vanhoorelbeke. (2022) Improvement of recombinant ADAMTS13 production through a more optimal signal peptide or an N‐terminal fusion protein. Journal of Thrombosis and Haemostasis 20:10, pages 2379-2385.
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Jong-Ho Park, Hoon-Min Lee, Eun-Ju Jin, Eun-Ji Lee, Yeon-Ju Kang, Sungkyun Kim, Sung-Sick Yoo, Gyun Min Lee & Yeon-Gu Kim. (2022) Development of an in vitro screening system for synthetic signal peptide in mammalian cell-based protein production. Applied Microbiology and Biotechnology 106:9-10, pages 3571-3582.
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Peng Yang, Yang Yang, Yuming Wu, Cong Huang, Yanlei Ding, Xuejun Wang & Shengqi Wang. (2021) An optimized and robust SARS-CoV-2 pseudovirus system for viral entry research. Journal of Virological Methods 295, pages 114221.
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Kai-Wen ChengFeng WangGeorge A. LopezSrikanth SingamsettyJill WoodPatricia I. DicksonTsui-Fen Chou. (2021) Evaluation of artificial signal peptides for secretion of two lysosomal enzymes in CHO cells. Biochemical Journal 478:12, pages 2309-2319.
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Elisabeth Andersen, Maria Eugenia Chollet, Marcello Baroni, Mirko Pinotti, Francesco Bernardi, Ellen Skarpen, Per Morten Sandset & Grethe Skretting. (2019) The effect of the chemical chaperone 4-phenylbutyrate on secretion and activity of the p.Q160R missense variant of coagulation factor FVII. Cell & Bioscience 9:1.
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Jian'er Lin, Shu Hui Neo, Steven C. L. Ho, Jessna H. M. Yeo, Tianhua Wang, Wei Zhang, Xuezhi Bi, Sheng-Hao Chao & Yuansheng Yang. (2017) Impact of Signal Peptides on Furin-2A Mediated Monoclonal Antibody Secretion in CHO Cells. Biotechnology Journal 12:9, pages 1700268.
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