2,990
Views
38
CrossRef citations to date
0
Altmetric
Commentary

The growing impact of lyophilized cell-free protein expression systems

, , &
Pages 325-330 | Received 06 Sep 2016, Accepted 22 Sep 2016, Published online: 28 Oct 2016

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (1)

Antonio Palazzo & René Massimiliano Marsano. (2021) Transposable elements: a jump toward the future of expression vectors. Critical Reviews in Biotechnology 41:5, pages 792-808.
Read now

Articles from other publishers (37)

Rani Brooks, Lisa Morici & Nicholas Sandoval. (2023) Cell Free Bacteriophage Synthesis from Engineered Strains Improves Yield. ACS Synthetic Biology 12:8, pages 2418-2431.
Crossref
Yanqiu Gu, Fang Fan, Yue Liu, Yifeng Chai, Yongfang Yuan & Xiaofei Chen. (2023) Cell-free protein synthesis system for bioanalysis: Advances in methods and applications. TrAC Trends in Analytical Chemistry 161, pages 117015.
Crossref
Asher J. Williams, Katherine F. Warfel, Primit Desai, Jie Li, Jen-Jie Lee, Derek A. Wong, Phuong M. Nguyen, Yufan Qin, Sarah E. Sobol, Michael C. Jewett, Yung-Fu Chang & Matthew P. DeLisa. (2023) A low-cost recombinant glycoconjugate vaccine confers immunogenicity and protection against enterotoxigenic Escherichia coli infections in mice. Frontiers in Molecular Biosciences 10.
Crossref
Katherine F. Warfel, Asher Williams, Derek A. Wong, Sarah E. Sobol, Primit Desai, Jie Li, Yung-Fu Chang, Matthew P. DeLisa, Ashty S. Karim & Michael C. Jewett. (2022) A Low-Cost, Thermostable, Cell-Free Protein Synthesis Platform for On-Demand Production of Conjugate Vaccines. ACS Synthetic Biology 12:1, pages 95-107.
Crossref
Mercedes Sánchez-Costa & Fernando López-Gallego. 2023. Cell-free Macromolecular Synthesis. Cell-free Macromolecular Synthesis 21 46 .
Eugenia Romantseva, Nina Alperovich, David Ross, Steven P Lund & Elizabeth A Strychalski. (2022) Effects of DNA template preparation on variability in cell-free protein production. Synthetic Biology 7:1.
Crossref
Huaipan Jiang, Jian Wang, Weilin Cong, Yihe Huang, Morteza Ramezani, Anup Sarma, Nikolay V. Dokholyan, Mehrdad Mahdavi & Mahmut T. Kandemir. (2022) Predicting Protein–Ligand Docking Structure with Graph Neural Network. Journal of Chemical Information and Modeling 62:12, pages 2923-2932.
Crossref
Yasuhiro Shimane & Yutetsu Kuruma. (2022) Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction. Frontiers in Bioengineering and Biotechnology 10.
Crossref
David K. Karig, Allison Reno, Lauren Elizabeth Franklin & Andrea C. Timm. 2022. Handbook of Cell Biosensors. Handbook of Cell Biosensors 319 338 .
Eugenia F. Romantseva, Drew S. Tack, Nina Alperovich, David Ross & Elizabeth A. Strychalski. 2022. Cell-Free Gene Expression. Cell-Free Gene Expression 3 50 .
Caroline E. Copeland, Adam Langlois, Jeehye Kim & Yong-Chan Kwon. (2021) The cell-free system: A new apparatus for affordable, sensitive, and portable healthcare. Biochemical Engineering Journal 175, pages 108124.
Crossref
Mehran Soltani, J. Porter Hunt & Bradley C. Bundy. (2021) Rapid RNase inhibitor production to enable low‐cost, on‐demand cell‐free protein synthesis biosensor use in human body fluids. Biotechnology and Bioengineering 118:10, pages 3973-3983.
Crossref
Jasmine M. Hershewe, Katherine F. Warfel, Shaelyn M. Iyer, Justin A. Peruzzi, Claretta J. Sullivan, Eric W. Roth, Matthew P. DeLisa, Neha P. Kamat & Michael C. Jewett. (2021) Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles. Nature Communications 12:1.
Crossref
Favour Danladi Makurvet. (2021) Biologics vs. small molecules: Drug costs and patient access. Medicine in Drug Discovery 9, pages 100075.
Crossref
Nan Jiang, Xuanwei Ding & Yuan Lu. (2021) Development of a robust Escherichia coli-based cell-free protein synthesis application platform. Biochemical Engineering Journal 165, pages 107830.
Crossref
Stephanie D. Cole, Aleksandr E. Miklos, Abel C. Chiao, Zachary Z. Sun & Matthew W. Lux. (2020) Methodologies for preparation of prokaryotic extracts for cell-free expression systems. Synthetic and Systems Biotechnology 5:4, pages 252-267.
Crossref
Nan Jiang, Lianju Ma & Yuan Lu. (2020) Cell-free synthetic biology in the new era of enzyme engineering. Chinese Journal of Chemical Engineering 28:11, pages 2810-2816.
Crossref
Xiaocui Guo, Yi Zhu, Lihui Bai & Dayong Yang. (2020) The Protection Role of Magnesium Ions on Coupled Transcription and Translation in Lyophilized Cell-Free System. ACS Synthetic Biology 9:4, pages 856-863.
Crossref
J. Porter Hunt, Kristen M. Wilding, R. Jordan Barnett, Hannah Robinson, Mehran Soltani, Jae Eun Cho & Bradley C. Bundy. (2020) Engineering Cell‐Free Protein Synthesis for High‐Yield Production and Human Serum Activity Assessment of Asparaginase: Toward On‐Demand Treatment of Acute Lymphoblastic Leukemia. Biotechnology Journal 15:4, pages 1900294.
Crossref
David K. Karig, Allison Reno, Lauren Elizabeth Franklin & Andrea C. Timm. 2020. Handbook of Cell Biosensors. Handbook of Cell Biosensors 1 20 .
J. Porter Hunt, Emily Long Zhao, Mehran Soltani, Madison Frei, J. Andrew D. Nelson & Bradley C. Bundy. (2019) Streamlining the preparation of “endotoxin-free” ClearColi cell extract with autoinduction media for cell-free protein synthesis of the therapeutic protein crisantaspase. Synthetic and Systems Biotechnology 4:4, pages 220-224.
Crossref
Benjamin J. Des Soye, Vincent R. Gerbasi, Paul M. Thomas, Neil L. Kelleher & Michael C. Jewett. (2019) A Highly Productive, One-Pot Cell-Free Protein Synthesis Platform Based on Genomically Recoded Escherichia coli. Cell Chemical Biology 26:12, pages 1743-1754.e9.
Crossref
Denis Tamiev, Alyssa Lantz, Grace Vezeau, Howard Salis & Nigel F. Reuel. (2019) Controlling Heterogeneity and Increasing Titer from Riboswitch-Regulated Bacillus subtilis Spores for Time-Delayed Protein Expression Applications . ACS Synthetic Biology 8:10, pages 2336-2346.
Crossref
David Garenne & Vincent Noireaux. (2019) Cell-free transcription–translation: engineering biology from the nanometer to the millimeter scale. Current Opinion in Biotechnology 58, pages 19-27.
Crossref
Seung-Ook Yang, Gregory H. Nielsen, Kristen M. Wilding, Merideth A. Cooper, David W. Wood & Bradley C. Bundy. (2019) Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator. Methods and Protocols 2:2, pages 52.
Crossref
Hye Jin Lim & Dong-Myung Kim. (2019) Cell-Free Metabolic Engineering: Recent Developments and Future Prospects. Methods and Protocols 2:2, pages 33.
Crossref
Xing Jin, Weston Kightlinger & Seok Hoon Hong. (2019) Optimizing Cell-Free Protein Synthesis for Increased Yield and Activity of Colicins. Methods and Protocols 2:2, pages 28.
Crossref
Raúl Manzano-Román & Manuel Fuentes. (2019) A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access. Journal of Proteomics 198, pages 27-35.
Crossref
Nicole E. Gregorio, Max Z. Levine & Javin P. Oza. (2019) A User’s Guide to Cell-Free Protein Synthesis. Methods and Protocols 2:1, pages 24.
Crossref
Yuan Lu. 2019. Current Developments in Biotechnology and Bioengineering. Current Developments in Biotechnology and Bioengineering 23 45 .
Bradley C Bundy, J Porter Hunt, Michael C Jewett, James R Swartz, David W Wood, Douglas D Frey & Govind Rao. (2018) Cell-free biomanufacturing. Current Opinion in Chemical Engineering 22, pages 177-183.
Crossref
Kristen M Wilding, Song-Min Schinn, Emily A Long & Bradley C Bundy. (2018) The emerging impact of cell-free chemical biosynthesis. Current Opinion in Biotechnology 53, pages 115-121.
Crossref
Mehran Soltani, Brady R. Davis, Hayley Ford, J. Andrew D. Nelson & Bradley C. Bundy. (2018) Reengineering cell-free protein synthesis as a biosensor: Biosensing with transcription, translation, and protein-folding. Biochemical Engineering Journal 138, pages 165-171.
Crossref
Benjamin J. Des Soye, Samuel R. Davidson, Matthew T. Weinstock, Daniel G. Gibson & Michael C. Jewett. (2018) Establishing a High-Yielding Cell-Free Protein Synthesis Platform Derived from Vibrio natriegens . ACS Synthetic Biology 7:9, pages 2245-2255.
Crossref
Sanchita Bhadra, Arti Pothukuchy, Raghav Shroff, Austin W. Cole, Michelle Byrom, Jared W. Ellefson, Jimmy D. Gollihar & Andrew D. Ellington. (2018) Cellular reagents for diagnostics and synthetic biology. PLOS ONE 13:8, pages e0201681.
Crossref
Amin S.M. Salehi, Seung Ook Yang, Conner C. Earl, Miriam J. Shakalli Tang, J. Porter Hunt, Mark T. Smith, David W. Wood & Bradley C. Bundy. (2018) Biosensing estrogenic endocrine disruptors in human blood and urine: A RAPID cell-free protein synthesis approach. Toxicology and Applied Pharmacology 345, pages 19-25.
Crossref
J. Porter Hunt, Song-Min Schinn, Matthew D. Jones & Bradley C. Bundy. (2017) Rapid, portable detection of endocrine disrupting chemicals through ligand-nuclear hormone receptor interactions. The Analyst 142:24, pages 4595-4600.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.