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Research Paper

Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes

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Pages 150-161 | Received 27 Feb 2019, Accepted 27 Apr 2019, Published online: 13 May 2019

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Lin Cui, Huoqing Huang, Honglian Zhang, Xiaolu Wang, Xing Qin, Tao Tu, Jie Zhang, Xiaoyun Su, Huimin Yu, Yingguo Bai, Huiying Luo, Bin Yao & Yuan Wang. (2022) Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner in Pichia pastoris. Bioengineered 13:5, pages 13860-13871.
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Jinjin Huang, Qingyi Zhao, Lingxiao Chen, Chunmei Zhang, Wei Bu, Xin Zhang, Kaini Zhang & Zhen Yang. (2020) Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastoris, which triggered ER stress. Bioengineered 11:1, pages 375-385.
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Edgar Velastegui, Johan Quezada, Karlo Guerrero, Claudia Altamirano, Juan Andres Martinez, Julio Berrios & Patrick Fickers. (2023) Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?. Applied Microbiology and Biotechnology 107:7-8, pages 2223-2233.
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Ana Lucía Herrera-Estala, José Antonio Fuentes-Garibay, Martha Guerrero-Olazarán & José María Viader-Salvadó. (2022) Low specific growth rate and temperature in fed-batch cultures of a beta-propeller phytase producing Pichia pastoris strain under GAP promoter trigger increased KAR2 and PSA1-1 gene expression yielding enhanced extracellular productivity. Journal of Biotechnology 352, pages 59-67.
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