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Journal of Liquid Chromatography & Related Technologies Volume 26, 2003 - Issue 5
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Original Articles

Studies on Renaturation with Simultaneous Purification of Recombinant Human Proinsulin from E. coli with High Performance Hydrophobic Interaction Chromatography

Quan Bai Institute of Modern Separation Science, Kay Lab of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an, 710069, P.R. ChinaCorrespondence[email protected]
,
Yu Kong Institute of Modern Separation Science, Kay Lab of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an, 710069, P.R. China
&
Xin‐du Geng Institute of Modern Separation Science, Kay Lab of Modern Separation Science in Shaanxi Province, Northwest University, Xi'an, 710069, P.R. China
Pages 683-695 | Received 14 Aug 2002, Accepted 14 Oct 2002, Published online: 06 Feb 2007

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Ruiying Zhang, Lu Zhang, Cong Li, Bang Chen, Qing Li, Xuexun Fang & Yehua Shen. (2015) Refolding of Recombinant Histidine-Tagged Catalytic Domain of MMP-13 from Escherichia coli with Ion-Exchange Chromatography for Higher Bioactivity. Journal of Liquid Chromatography & Related Technologies 38:5, pages 561-568.
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Articles from other publishers (15)

Fatemeh Khosravi, Mansi Upadhyay, Ashutosh Kumar, Mohammad Bagher Shahsavani, Mohsen Akbarian, Haniyeh Najafi, Ali Mohammad Tamaddon & Reza Yousefi. (2022) A novel method for the chaperone aided and efficient production of human proinsulin in the prokaryotic system. Journal of Biotechnology 346, pages 35-46.
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Yin Yin Siew & Wei Zhang. (2021) Downstream processing of recombinant human insulin and its analogues production from E. coli inclusion bodies. Bioresources and Bioprocessing 8:1.
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Ganapathy SubramanianAnurag S. Rathore, Nikhil Kateja & Harshit Agarwal. 2017. Continuous Biomanufacturing - Innovative Technologies and Methods. Continuous Biomanufacturing - Innovative Technologies and Methods 259 288 .
Sandeep Vemula, Sushma Vemula, Akshay Dedaniya & Srinivasa Reddy Ronda. (2015) In vitro refolding with simultaneous purification of recombinant human parathyroid hormone (rhPTH 1–34) from Escherichia coli directed by protein folding size exclusion chromatography (PF-SEC): implication of solution additives and their role on aggregates and renaturation. Analytical and Bioanalytical Chemistry 408:1, pages 217-229.
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Yan Wang, Wenxuan Ren, Dong Gao, Lili Wang, Ying Yang & Quan Bai. (2015) One-step refolding and purification of recombinant human tumor necrosis factor- α (rhTNF- α ) using ion-exchange chromatography . Biomedical Chromatography 29:2, pages 305-311.
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Fei Wang, Jinjin Guo, Quan Bai & Lili Wang. (2014) Refolding and purification of recombinant human (Pro)renin receptor from Escherichia coli by ion exchange chromatography . Biotechnology Progress 30:4, pages 864-871.
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Martin Wellhoefer, Wolfgang Sprinzl, Rainer Hahn & Alois Jungbauer. (2013) Continuous processing of recombinant proteins: Integration of inclusion body solubilization and refolding using simulated moving bed size exclusion chromatography with buffer recycling. Journal of Chromatography A 1319, pages 107-117.
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Beckley K. Nfor, Esteban J. Freydell & Marcel Ottens. 2010. Pharmaceutical Sciences Encyclopedia. Pharmaceutical Sciences Encyclopedia 1 30 .
Quan Bai & Xindu Geng. 2011. Heterologous Gene Expression in E.coli. Heterologous Gene Expression in E.coli 69 85 .
Esteban J. Freydell, Luuk van der Wielen, Michel Eppink & Marcel Ottens. (2010) Ion-exchange chromatographic protein refolding. Journal of Chromatography A 1217:46, pages 7265-7274.
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Esteban J. Freydell, Yvonne Bulsink, Stef van Hateren, Luuk van der Wielen, Michel Eppink & Marcel Ottens. (2010) Size-exclusion simulated moving bed chromatographic protein refolding. Chemical Engineering Science 65:16, pages 4701-4713.
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Xindu Geng & Lili Wang. (2008) Liquid chromatography of recombinant proteins and protein drugs. Journal of Chromatography B 866:1-2, pages 133-153.
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Paul A. O'Farrell. 2008. Molecular Biomethods Handbook. Molecular Biomethods Handbook 731 739 .
Sudhir Sahdev, Sunil K. Khattar & Kulvinder Singh Saini. (2007) Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies. Molecular and Cellular Biochemistry 307:1-2, pages 249-264.
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Xindu Geng & Chaozhan Wang. (2007) Protein folding liquid chromatography and its recent developments. Journal of Chromatography B 849:1-2, pages 69-80.
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