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Preparative Biochemistry & Biotechnology Volume 34, 2004 - Issue 3
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Original Articles

Fusion Expression of Human Beta‐Defensin‐2 from Multiple Joined Genes in Escherichia coli

Fang Wang Department of Chemical Engineering and Bioengineering, Institute of Bioengineering, Zhejiang University, Hangzhou, 310027, P.R. China
,
Xiangming Fang The Central Laboratory of Sir Run Run Shaw Hospital, School of Medical Science, Zhejiang University, Hangzhou, P.R. China
,
Zhinan Xu Department of Chemical Engineering and Bioengineering, Institute of Bioengineering, Zhejiang University, Hangzhou, 310027, P.R. ChinaCorrespondence[email protected]
,
Li Peng Department of Chemical Engineering and Bioengineering, Institute of Bioengineering, Zhejiang University, Hangzhou, 310027, P.R. China
&
Peilin Cen Department of Chemical Engineering and Bioengineering, Institute of Bioengineering, Zhejiang University, Hangzhou, 310027, P.R. China
Pages 215-225 | Received 09 Jun 2003, Accepted 12 Jul 2003, Published online: 18 Aug 2006

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J. Wang, J. Chen, R. Xu & Z. Xu. (2008) Batch and Fed-Batch Cultivation for Excretive Production of Human Epidermal Growth Factor (hEGF) with Recombinant E. Coli K12 System. Preparative Biochemistry & Biotechnology 38:3, pages 271-281.
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Articles from other publishers (16)

Ramon Roca-Pinilla, Leszek Lisowski, Anna Arís & Elena Garcia-Fruitós. (2022) The future of recombinant host defense peptides. Microbial Cell Factories 21:1.
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Paulina Díaz-Garrido, Rosa Elena Cárdenas-Guerra, Ignacio Martínez, Sebastián Poggio, Karla Rodríguez-Hernández, Lucio Rivera-Santiago, Jaime Ortega-López, Sergio Sánchez-Esquivel & Bertha Espinoza. (2021) Differential activity on trypanosomatid parasites of a novel recombinant defensin type 1 from the insect Triatoma (Meccus) pallidipennis. Insect Biochemistry and Molecular Biology 139, pages 103673.
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Xiuxia Su, Wenjing Huo, Dong Yang, Chonglin Luan & Jia Xu. (2019) Label-free liquid crystal immunosensor for detection of HBD-2. Talanta 203, pages 203-209.
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Baojian Hang, Jianfeng Pan, Desheng Ni, Qiang Zheng, Xu Zhang, Jin Cai, Lei Huang, Peilian Wei & Zhinan Xu. (2016) High-level production of aquaporin Z in Escherichia coli using maltose-binding protein/polyhistidine dual-affinity tag fusion system. Process Biochemistry 51:5, pages 599-606.
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Ligia Corrales-Garcia, Ernesto Ortiz, Julio Castañeda-Delgado, Bruno Rivas-Santiago & Gerardo Corzo. (2013) Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis. Protein Expression and Purification 89:1, pages 33-43.
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L. L. Corrales-Garcia, L. D. Possani & G. Corzo. (2010) Expression systems of human β-defensins: vectors, purification and biological activities. Amino Acids 40:1, pages 5-13.
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Yifeng Li. (2009) Carrier proteins for fusion expression of antimicrobial peptides in Escherichia coli . Biotechnology and Applied Biochemistry 54:1, pages 1-9.
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Lei Huang, Zhinan Xu, Zhixia Zhong, Li Peng, Haiqin Chen & Peilin Cen. (2007) Enhanced Expression and Primary Purification of Soluble HBD3 Fusion Protein in Escherichia coli. Applied Biochemistry and Biotechnology 142:2, pages 139-147.
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Hua Diao, Chenyun Guo, Donghai Lin & Yonglian Zhang. (2007) Intein-mediated expression is an effective approach in the study of β-defensins. Biochemical and Biophysical Research Communications 357:4, pages 840-846.
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Marzena Pazgier & Jacek Lubkowski. (2006) Expression and purification of recombinant human α-defensins in Escherichia coli. Protein Expression and Purification 49:1, pages 1-8.
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Zhinan Xu, Zhixia Zhong, Lei Huang, Li Peng, Fang Wang & Peilin Cen. (2006) High-level production of bioactive human beta-defensin-4 in Escherichia coli by soluble fusion expression. Applied Microbiology and Biotechnology 72:3, pages 471-479.
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Zhixia Zhong, Zhinan Xu, Li Peng, Lei Huang, Xiangming Fang & Peilin Cen. (2005) Tandem repeat mhBD2 gene enhance the soluble fusion expression of hBD2 in Escherichia coli. Applied Microbiology and Biotechnology 71:5, pages 661-667.
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Lei Huang, Jufang Wang, Zhixia Zhong, Li Peng, Haiqin Chen, Zhinan Xu & Peilin Cen. (2006) Production of Bioactive Human β-defensin-3 in Escherichia coli by Soluble Fusion Expression. Biotechnology Letters 28:9, pages 627-632.
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Haiqin Chen, Zhinan Xu, Li Peng, Xiangming Fang, Xiufei Yin, Naizheng Xu & Peilin Cen. (2006) Recent advances in the research and development of human defensins. Peptides 27:4, pages 931-940.
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Enno Klüver, Knut Adermann & Axel Schulz. (2006) Synthesis and structure–activity relationship of β-defensins, multi-functional peptides of the immune system. Journal of Peptide Science 12:4, pages 243-257.
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Brian C. Bryksa, Lisa D. MacDonald, Aleks Patrzykat, Susan E. Douglas & Neil R. Mattatall. (2006) A C-terminal glycine suppresses production of pleurocidin as a fusion peptide in Escherichia coli. Protein Expression and Purification 45:1, pages 88-98.
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