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Journal of Receptors and Signal Transduction Volume 22, 2002 - Issue 1-4

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Research Article

A STOPPED-FLOW FLUORESCENCE ANISOTROPY METHOD FOR MEASURING HORMONE BINDING AND DISSOCIATION KINETICS WITH CELL-SURFACE RECEPTORS IN LIVING CELLS

John C. Wilkinson Department of Biological Sciences, Vanderbilt University, Nashville, TN, 37235, U.S.A.; Department of Pathology, University of Michigan, Ann Arbor, MI, 48109, U.S.A.

Joseph M. Beechem Molecular Probes, Inc., Eugene, OR, 97402, U.S.A.

James V. Staros Department of Biological Sciences, Vanderbilt University, Nashville, TN, 37235, U.S.A.; Department of Biochemistry and Cell Biology, SUNY-Stony Brook, Stony Brook, NY, 11794-5215, U.S.A.

, PhD

Pages 357-371 | Published online: 11 Nov 2002

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https://doi.org/10.1081/RRS-120014607

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Roberto F. Delgadillo, Katie A. Carnes, Nestor Valles-Villarreal, Omar Olmos, Kathia Zaleta-Rivera & Lawrence J. Parkhurst. (2020) Dual-Channel Stopped-Flow Apparatus for Simultaneous Fluorescence, Anisotropy, and FRET Kinetic Data Acquisition for Binary and Ternary Biological Complexes. Biosensors 10:11, pages 180.
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Andrew Moreno, J. L. Knee & Ishita Mukerji. (2016) Photophysical Characterization of Enhanced 6-Methylisoxanthopterin Fluorescence in Duplex DNA. The Journal of Physical Chemistry B 120:48, pages 12232-12248.
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Jérôme Quérard, Thomas Le Saux, Arnaud Gautier, Damien Alcor, Vincent Croquette, Annie Lemarchand, Charlie Gosse & Ludovic Jullien. (2016) Kinetics of Reactive Modules Adds Discriminative Dimensions for Selective Cell Imaging. ChemPhysChem 17:10, pages 1396-1413.
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Ambadas B. Rode, Tamaki Endoh, Hisae Tateishi-Karimata, Shuntaro Takahashi & Naoki Sugimoto. (2013) Real-time monitoring of DNA hybridization kinetics on living cell surfaces. Chemical Communications 49:76, pages 8444.
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Hairong Ma, Emily A. Gibson, Philip J. Dittmer, Ralph Jimenez & Amy E. Palmer. (2012) High-Throughput Examination of Fluorescence Resonance Energy Transfer-Detected Metal-Ion Response in Mammalian Cells. Journal of the American Chemical Society 134:5, pages 2488-2491.
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Michael Vibo Grandal & Inger Helene Madshus. (2008) Epidermal growth factor receptor and cancer: control of oncogenic signalling by endocytosis. Journal of Cellular and Molecular Medicine 12:5a, pages 1527-1534.
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Kristin B. WhitsonStefanie R. WhitsonMonica L. Red-BrewerAustin J. McCoyAngela A. VitaliFrancesca WalkerTerrance G. JohnsAlbert H. BethJames V. Staros. (2005) Functional Effects of Glycosylation at Asn-579 of the Epidermal Growth Factor Receptor. Biochemistry 44:45, pages 14920-14931.
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Aleeta M. Powe, Kristin A. Fletcher, Nadia N. St. Luce, Mark Lowry, Sharon Neal, Matthew E. McCarroll, Philip B. Oldham, Linda B. McGown & Isiah M. Warner. (2004) Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry. Analytical Chemistry 76:16, pages 4614-4634.
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Kristin B Whitson, Joseph M Beechem, Albert H Beth & James V Staros. (2004) Preparation and characterization of Alexa Fluor 594-labeled epidermal growth factor for fluorescence resonance energy transfer studies: application to the epidermal growth factor receptor. Analytical Biochemistry 324:2, pages 227-236.
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A STOPPED-FLOW FLUORESCENCE ANISOTROPY METHOD FOR MEASURING HORMONE BINDING AND DISSOCIATION KINETICS WITH CELL-SURFACE RECEPTORS IN LIVING CELLS

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A STOPPED-FLOW FLUORESCENCE ANISOTROPY METHOD FOR MEASURING HORMONE BINDING AND DISSOCIATION KINETICS WITH CELL-SURFACE RECEPTORS IN LIVING CELLS

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