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Molecular and Cellular Biology Volume 34, 2014 - Issue 12
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Article

Unraveling the Complexities of DNA-Dependent Protein Kinase Autophosphorylation

Jessica A. Neala College of Veterinary Medicine, Department of Microbiology and Molecular Genetics, and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan, USA
,
Seiji Sugiman-Marangosb Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
,
Pamela VanderVere-Carozzac Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA
,
Mike Wagnerc Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA;d Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, Indiana, USA
,
John Turchic Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana, USA;e Departments of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, USA
,
Susan P. Lees-Millerf Departments of Biochemistry and Molecular Biology and Oncology, University of Calgary, Calgary, Alberta, Canada
,
Murray S. Junopb Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada
&
Katheryn Meeka College of Veterinary Medicine, Department of Microbiology and Molecular Genetics, and Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan, USACorrespondence[email protected]
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Pages 2162-2175 | Received 25 Nov 2013, Accepted 26 Mar 2014, Published online: 20 Mar 2023

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Sunetra Roy, Abinadabe J. de Melo, Yao Xu, Satish K. Tadi, Aurélie Négrel, Eric Hendrickson, Mauro Modesti & Katheryn Meek. (2015) XRCC4/XLF Interaction Is Variably Required for DNA Repair and Is Not Required for Ligase IV Stimulation. Molecular and Cellular Biology 35:17, pages 3017-3028.
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Natividad Gomez-Roman, Ming Y Chong, Sandeep K. Chahal, Seamus P. Caragher, Mark R. Jackson, Katrina H. Stevenson, Sidhartha A. Dongre & Anthony J. Chalmers. (2020) Radiation Responses of 2D and 3D Glioblastoma Cells: A Novel, 3D-specific Radioprotective Role of VEGF/Akt Signaling through Functional Activation of NHEJ. Molecular Cancer Therapeutics 19:2, pages 575-589.
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Crossref
Huiming Lu, Janapriya Saha, Pauline J Beckmann, Eric A Hendrickson & Anthony J Davis. (2019) DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response. Nucleic Acids Research 47:18, pages 9467-9479.
Crossref
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Crossref
Benjamin Schrank & Jean Gautier. (2019) Assembling nuclear domains: Lessons from DNA repair. Journal of Cell Biology 218:8, pages 2444-2455.
Crossref
Aleš Hnízda & Tom L Blundell. (2019) Multicomponent assemblies in DNA-double-strand break repair by NHEJ. Current Opinion in Structural Biology 55, pages 154-160.
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Mingjie Liu, Saad Rehman, Xidian Tang, Kui Gu, Qinlei Fan, Dekun Chen & Wentao Ma. (2019) Methodologies for Improving HDR Efficiency. Frontiers in Genetics 9.
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Benjamin R. Nixon, Sara C. Sebag, Michael S. Glennon, Eric J. Hall, Emily S. Kounlavong, Michael L. Freeman & Jason R. Becker. (2018) Nuclear localized Raf1 isoform alters DNA‐dependent protein kinase activity and the DNA damage response. The FASEB Journal 33:1, pages 1138-1150.
Crossref
Jessica A. Neal & Katheryn Meek. (2019) Deciphering phenotypic variance in different models of DNA-PKcs deficiency. DNA Repair 73, pages 7-16.
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Crossref
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Crossref
Edward J. Bartlett & Susan P. Lees-Miller. 2018. Targeting the DNA Damage Response for Anti-Cancer Therapy. Targeting the DNA Damage Response for Anti-Cancer Therapy 315 338 .
Songli Zhu, Laura A. Fisher, Tadayoshi Bessho & Aimin Peng. (2017) Protein phosphatase 1 and phosphatase 1 nuclear targeting subunit-dependent regulation of DNA-dependent protein kinase and non-homologous end joining. Nucleic Acids Research 45:18, pages 10583-10594.
Crossref
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Crossref
Davide Normanno, Aurélie Négrel, Abinadabe J de Melo, Stéphane Betzi, Katheryn Meek & Mauro Modesti. (2017) Mutational phospho-mimicry reveals a regulatory role for the XRCC4 and XLF C-terminal tails in modulating DNA bridging during classical non-homologous end joining. eLife 6.
Crossref
Guillermo Sastre-Moreno, John M. Pryor, Marta Moreno-Oñate, Andrés M. Herrero-Ruiz, Felipe Cortés-Ledesma, Luis Blanco, Dale A. Ramsden & Jose F. Ruiz. (2017) Regulation of human polλ by ATM-mediated phosphorylation during non-homologous end joining. DNA Repair 51, pages 31-45.
Crossref
Susan P Lees-Miller, Tara L Beattie & John A Tainer. (2016) Noncoding RNA joins Ku and DNA-PKcs for DNA-break resistance in breast cancer. Nature Structural & Molecular Biology 23:6, pages 509-510.
Crossref
Emil Mladenov, Simon Magin, Aashish Soni & George Iliakis. (2016) DNA double-strand-break repair in higher eukaryotes and its role in genomic instability and cancer: Cell cycle and proliferation-dependent regulation. Seminars in Cancer Biology 37-38, pages 51-64.
Crossref
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Crossref
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Crossref
Payel Chatterjee, Gaurav S. Choudhary, Turkeyah Alswillah, Xiahui Xiong, Warren D. Heston, Cristina Magi-Galluzzi, Junran Zhang, Eric A. Klein & Alexandru Almasan. (2015) The TMPRSS2–ERG Gene Fusion Blocks XRCC4-Mediated Nonhomologous End-Joining Repair and Radiosensitizes Prostate Cancer Cells to PARP Inhibition. Molecular Cancer Therapeutics 14:8, pages 1896-1906.
Crossref
Lian-Hong Zou, Zeng-Fu Shang, Wei Tan, Xiao-Dan Liu, Qin-Zhi Xu, Man Song, Yu Wang, Hua Guan, Shi-Meng Zhang, Lan Yu, Cai-Gao Zhong & Ping-Kun Zhou. (2015) TNKS1BP1 functions in DNA double-strand break repair though facilitating DNA-PKcs autophosphorylation dependent on PARP-1. Oncotarget 6:9, pages 7011-7022.
Crossref
Angel Rivera-Calzada, Andrés López-Perrote, Roberto Melero, Jasminka Boskovic, Hugo Muñoz-Hernández, Fabrizio Martino & Oscar Llorca. (2015) Structure and Assembly of the PI3K-like Protein Kinases (PIKKs) Revealed by Electron Microscopy. AIMS Biophysics 2:2, pages 36-57.
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Crossref

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