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Molecular and Cellular Biology Volume 16, 1996 - Issue 9
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Research Article

Protein-Protein and Protein-DNA Interaction Regions within the DNA End-Binding Protein Ku70-Ku86

Xiantuo WuDivision of Molecular Oncology and Center for Immunology, Departments of Pathology and of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri63110
&
Michael R. LieberDivision of Molecular Oncology and Center for Immunology, Departments of Pathology and of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri63110Correspondence[email protected]
Pages 5186-5193 | Received 23 Feb 1996, Accepted 05 Jun 1996, Published online: 29 Mar 2023

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C. Caney, G. Singh, H. Lukka & A. J. Rainbow. (2004) Combined gamma‐irradiation and subsequent cisplatin treatment in human squamous carcinoma cell lines sensitive and resistant to cisplatin. International Journal of Radiation Biology 80:4, pages 291-299.
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Dan Gebow, Nathan Miselis & Howard L. Liber. (2000) Homologous and Nonhomologous Recombination Resulting in Deletion: Effects of p53 Status, Microhomology, and Repetitive DNA Length and Orientation. Molecular and Cellular Biology 20:11, pages 4028-4035.
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Renu Tuteja & Narendra Tuteja. (2000) Ku Autoantigen: A Multifunctional DNA-Binding Protein. Critical Reviews in Biochemistry and Molecular Biology 35:1, pages 1-33.
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Ward Giffin, Wenrong Gong, Caroline Schild-Poulter & Robert J. G. Haché. (1999) Ku Antigen-DNA Conformation Determines the Activation of DNA-Dependent Protein Kinase and DNA Sequence-Directed Repression of Mouse Mammary Tumor Virus Transcription. Molecular and Cellular Biology 19:6, pages 4065-4078.
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B. K. Singleton, M. I. Torres-Arzayus, S. T. Rottinghaus, G. E. Taccioli & P. A. Jeggo. (1999) The C Terminus of Ku80 Activates the DNA-Dependent Protein Kinase Catalytic Subunit. Molecular and Cellular Biology 19:5, pages 3267-3277.
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B. K. Singleton, A. Priestley, H. Steingrimsdottir, D. Gell, T. Blunt, S. P. Jackson, A. R. Lehmann & P. A. Jeggo. (1997) Molecular and Biochemical Characterization of xrs Mutants Defective in Ku80. Molecular and Cellular Biology 17:3, pages 1264-1273.
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Gabrielle J. Grundy, Hayley A. Moulding, Keith W. Caldecott & Stuart L. Rulten. (2014) One ring to bring them all—The role of Ku in mammalian non-homologous end joining. DNA Repair 17, pages 30-38.
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Purnata Shirodkar, Amanda L. Fenton, Li Meng & C. Anne Koch. (2013) Identification and Functional Characterization of a Ku-binding Motif in Aprataxin Polynucleotide Kinase/Phosphatase-like Factor (APLF). Journal of Biological Chemistry 288:27, pages 19604-19613.
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Chitra Subramanian, Manila Hada, Anthony W. OpipariJrJr, Valerie P. Castle & Roland P.S. Kwok. (2013) CREB-Binding Protein Regulates Ku70 Acetylation in Response to Ionization Radiation in Neuroblastoma. Molecular Cancer Research 11:2, pages 173-181.
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Shaowen Hu & Francis A. Cucinotta. (2011) Computational studies on full-length Ku70 with DNA duplexes: base interactions and a helical path. Journal of Molecular Modeling 18:5, pages 1935-1949.
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Yingfeng Zheng, Zhujun Ao, Binchen Wang, Kallesh Danappa Jayappa & Xiaojian Yao. (2011) Host Protein Ku70 Binds and Protects HIV-1 Integrase from Proteasomal Degradation and Is Required for HIV Replication. Journal of Biological Chemistry 286:20, pages 17722-17735.
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Seho Cha, Chunghun Lim, Jae Young Lee, Yoon-Jae Song, Junsoo Park, Joonho Choe & Taegun Seo. (2010) DNA-PK/Ku complex binds to latency-associated nuclear antigen and negatively regulates Kaposi’s sarcoma-associated herpesvirus latent replication. Biochemical and Biophysical Research Communications 394:4, pages 934-939.
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Ekaterina S. Ilina, Olga I. Lavrik & Svetlana N. Khodyreva. (2008) Ku antigen interacts with abasic sites. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1784:11, pages 1777-1785.
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Chang-Shi Chen, Yu-Chieh Wang, Hsiao-Ching Yang, Po-Hsien Huang, Samuel K. Kulp, Chih-Cheng Yang, Yen-Shen Lu, Shigemi Matsuyama, Ching-Yu Chen & Ching-Shih Chen. (2007) Histone Deacetylase Inhibitors Sensitize Prostate Cancer Cells to Agents that Produce DNA Double-Strand Breaks by Targeting Ku70 Acetylation. Cancer Research 67:11, pages 5318-5327.
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Les A. Hanakahi. (2007) 2-Step purification of the Ku DNA repair protein expressed in Escherichia coli. Protein Expression and Purification 52:1, pages 139-145.
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Longhou Fang, YiGuo Wang, Dan Du, Guang Yang, Tim Tak Kwok, Siu Kai Kong, Benjamin Chen, David J Chen & Zhengjun Chen. (2007) Cell polarity protein Par3 complexes with DNA-PK via Ku70 and regulates DNA double-strand break repair. Cell Research 17:2, pages 100-116.
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Charles Gullo, Melvin Au, Ge Feng & Gerrard Teoh. (2006) The biology of Ku and its potential oncogenic role in cancer. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer 1765:2, pages 223-234.
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Errol C. Friedberg, Graham C. Walker, Wolfram Siede, Richard D. Wood, Roger A. Schultz & Tom Ellenberger. 2005. DNA Repair and Mutagenesis. DNA Repair and Mutagenesis 711 750 .
Eric Hendrickson, Joy Huffman & John Tainer. 2005. DNA Damage Recognition. DNA Damage Recognition.
Manabu Koike & Aki Koike. (2005) The Ku70-binding site of Ku80 is required for the stabilization of Ku70 in the cytoplasm, for the nuclear translocation of Ku80, and for Ku80-dependent DNA repair. Experimental Cell Research 305:2, pages 266-276.
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Spencer J Collis, Theodore L DeWeese, Penelope A Jeggo & Antony R Parker. (2004) The life and death of DNA-PK. Oncogene 24:6, pages 949-961.
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Sean Rooney, Jayanta Chaudhuri & Frederick W. Alt. (2004) The role of the non-homologous end-joining pathway in lymphocyte development. Immunological Reviews 200:1, pages 115-131.
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Yuan LiuHui-I KaoRobert A. Bambara. (2004) Flap Endonuclease 1: A Central Component of DNA Metabolism. Annual Review of Biochemistry 73:1, pages 589-615.
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Joanna Łanuszewska & Piotr Widłak. (2004) The truncation of Ku86 in human lymphocytes. Cancer Letters 205:2, pages 197-205.
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Sihong Song, Yuanqing Lu, Young-Kook Choi, Yinong Han, Qiushi Tang, Ge Zhao, Kenneth I. Berns & Terence R. Flotte. (2004) DNA-dependent PK inhibits adeno-associated virus DNA integration. Proceedings of the National Academy of Sciences 101:7, pages 2112-2116.
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Manabu KOIKE & Aki KOIKE. (2004) The Establishment and Characterization of Cell Lines Stably Expressing Human Ku80 Tagged with Enhanced Green Fluorescent Protein. Journal of Radiation Research 45:1, pages 119-125.
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Joo Weon Lim, Hyeyoung Kim & Kyung Hwan Kim. (2004) The Ku Antigen-Recombination Signal-binding Protein Jκ Complex Binds to the Nuclear Factor-κB p50 Promoter and Acts as a Positive Regulator of p50 Expression in Human Gastric Cancer Cells. Journal of Biological Chemistry 279:1, pages 231-237.
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Rajat Roy, Bettina Meier, Andrew D. McAinsh, Heidi M. Feldmann & Stephen P. Jackson. (2004) Separation-of-function Mutants of Yeast Ku80 Reveal a Yku80p-Sir4p Interaction Involved in Telomeric Silencing. Journal of Biological Chemistry 279:1, pages 86-94.
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Fabrizio d'Adda di Fagagna, Geoffrey R Weller, Aidan J Doherty & Stephen P Jackson. (2003) The Gam protein of bacteriophage Mu is an orthologue of eukaryotic Ku. EMBO reports 4:1, pages 47-52.
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Jesse Bertinato, Julianna J Tomlinson, Caroline Schild-Poulter & Robert J.G Haché. (2003) Evidence implicating Ku antigen as a structural factor in RNA polymerase II-mediated transcription. Gene 302:1-2, pages 53-64.
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Chung-Hui Kim, Su-Jung Park & Suk-Hee Lee. (2002) A Targeted Inhibition of DNA-Dependent Protein Kinase Sensitizes Breast Cancer Cells Following Ionizing Radiation. Journal of Pharmacology and Experimental Therapeutics 303:2, pages 753-759.
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Joo Weon Lim, Hyeyoung Kim & Kyung Hwan Kim. (2002) Expression of Ku70 and Ku80 Mediated by NF-κB and Cyclooxygenase-2 Is Related to Proliferation of Human Gastric Cancer Cells. Journal of Biological Chemistry 277:48, pages 46093-46100.
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Won Jun Oh, Eun Kyung Kim, Jung Ho Ko, Seung Hee Yoo, Si Houn Hahn & Ook-Joon Yoo. (2002) Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene. European Journal of Biochemistry 269:8, pages 2151-2161.
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MANABU KOIKE. (2002) Dimerization, Translocation and Localization of Ku70 and Ku80 Proteins. Journal of Radiation Research 43:3, pages 223-236.
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Olivia Novac, Diamanto Matheos, Felipe D. Araujo, Gerald B. Price & Maria Zannis-Hadjopoulos. (2001) In Vivo Association of Ku with Mammalian Origins of DNA Replication. Molecular Biology of the Cell 12:11, pages 3386-3401.
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Aidan J. Doherty & Stephen P. Jackson. (2001) DNA repair: How Ku makes ends meet. Current Biology 11:22, pages R920-R924.
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Ziming Zhang, Lingyang Zhu, Donghai Lin, Fanqing Chen, David J. Chen & Yuan Chen. (2001) The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70. Journal of Biological Chemistry 276:41, pages 38231-38236.
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Manabu Koike, Tadahiro Shiomi & Aki Koike. (2001) Dimerization and Nuclear Localization of Ku Proteins. Journal of Biological Chemistry 276:14, pages 11167-11173.
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Kyuyoung Song, Yusun Jung, Donghae Jung & Inchul Lee. (2001) Human Ku70 Interacts with Heterochromatin Protein 1α. Journal of Biological Chemistry 276:11, pages 8321-8327.
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Baomin Li & Lucio Comai. (2001) Requirements for the Nucleolytic Processing of DNA Ends by the Werner Syndrome Protein-Ku70/80 Complex. Journal of Biological Chemistry 276:13, pages 9896-9902.
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Jesse Bertinato, Caroline Schild-Poulter & Robert J. G. Haché. (2001) Nuclear localization of Ku antigen is promoted independently by basic motifs in the Ku70 and Ku80 subunits. Journal of Cell Science 114:1, pages 89-99.
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Yu-Tzu Tai, Gerrard Teoh, Boris Lin, Faith E. Davies, Dharminder Chauhan, Steven P. Treon, Noopur Raje, Teru Hideshima, Yoshihito Shima, Klaus Podar & Kenneth C. Anderson. (2000) Ku86 Variant Expression and Function in Multiple Myeloma Cells Is Associated with Increased Sensitivity to DNA Damage. The Journal of Immunology 165:11, pages 6347-6355.
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Jutta M. Pleschke, Hanna E. Kleczkowska, Mark Strohm & Felix R. Althaus. (2000) Poly(ADP-ribose) Binds to Specific Domains in DNA Damage Checkpoint Proteins. Journal of Biological Chemistry 275:52, pages 40974-40980.
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Manabu Koike, Tadahiro Shiomi & Aki Koike. (2000) Ku70 Can Translocate to the Nucleus Independent of Ku80 Translocation and DNA-PK Autophosphorylation. Biochemical and Biophysical Research Communications 276:3, pages 1105-1111.
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Kyuyoung Song, Donghae Jung, Yusun Jung, Seong-Gene Lee & Inchul Lee. (2000) Interaction of human Ku70 with TRF2. FEBS Letters 481:1, pages 81-85.
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Sigrun Jaklin, Lucia Driller, Raymund J. Wellinger, Michel Larrivée, Elisabeth Kremmer & Heidi M. Feldmann. (2000) A Short C-terminal Domain of Yku70p Is Essential for Telomere Maintenance. Journal of Biological Chemistry 275:32, pages 24921-24927.
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Trevor Huyton, Paul A Bates, Xiaodong Zhang, Michael J.E Sternberg & Paul S Freemont. (2000) The BRCA1 C-terminal domain: structure and function. Mutation Research/DNA Repair 460:3-4, pages 319-332.
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E. Marangoni, J.O. Bay, P. Verrelle & J. Bourhis. (2000) Transfert de gène pour modifier la réponse à la radiothérapie ?. Cancer/Radiothérapie 4:3, pages 175-180.
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Manabu Koike, Togo Ikuta, Takashi Miyasaka & Tadahiro Shiomi. (1999) Ku80 can translocate to the nucleus independent of the translocation of Ku70 using its own nuclear localization signal. Oncogene 18:52, pages 7495-7505.
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Kazuhiko Yamane & Takashi Tsuruo. (1999) Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA. Oncogene 18:37, pages 5194-5203.
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Sunghan Yoo, Amy Kimzey & William S. Dynan. (1999) Photocross-linking of an Oriented DNA Repair Complex. Journal of Biological Chemistry 274:28, pages 20034-20039.
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