Advanced search
Publication Cover
Molecular and Cellular Biology Volume 17, 1997 - Issue 5
Submit an article Journal homepage
8
Views
52
CrossRef citations to date
0
Altmetric
Research Article

Novel Gal3 Proteins Showing Altered Gal80p Binding Cause Constitutive Transcription of Gal4p-Activated Genes in Saccharomyces cerevisiae

T. Eric BlankDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033;Cell and Molecular Biology Graduate Program, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033
,
Michael P. WoodsDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033;Intercollege Graduate Program in Genetics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033
,
Colleen M. LeboDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033
,
Ping XinDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033
&
James E. HopperDepartment of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033;Cell and Molecular Biology Graduate Program, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033;Intercollege Graduate Program in Genetics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania17033Correspondence[email protected]
Pages 2566-2575 | Received 23 Sep 1996, Accepted 24 Feb 1997, Published online: 29 Mar 2023

Citations (52)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (5)

Onur Egriboz, Sudip Goswami, Xiaorong Tao, Kathleen Dotts, Christie Schaeffer, Vepkhia Pilauri & James E. Hopper. (2013) Self-Association of the Gal4 Inhibitor Protein Gal80 Is Impaired by Gal3: Evidence for a New Mechanism in the GAL Gene Switch. Molecular and Cellular Biology 33:18, pages 3667-3674.
Read now
Fenglei Jiang, Benjamin R. Frey, Margery L. Evans, Jordan C. Friel & James E. Hopper. (2009) Gene Activation by Dissociation of an Inhibitor from a Transcriptional Activation Domain. Molecular and Cellular Biology 29:20, pages 5604-5610.
Read now
Gang Peng & James E. Hopper. (2000) Evidence for Gal3p's Cytoplasmic Location and Gal80p's Dual Cytoplasmic-Nuclear Location Implicates New Mechanisms for Controlling Gal4p Activity in Saccharomyces cerevisiae. Molecular and Cellular Biology 20:14, pages 5140-5148.
Read now
Alok Kumar Sil, Samina Alam, Ping Xin, Ly Ma, Melissa Morgan, Colleen M. Lebo, Michael P. Woods & James E. Hopper. (1999) The Gal3p-Gal80p-Gal4p Transcription Switch of Yeast: Gal3p Destabilizes the Gal80p-Gal4p Complex in Response to Galactose and ATP. Molecular and Cellular Biology 19:11, pages 7828-7840.
Read now
Angela Hach, Thomas Hon & Li Zhang. (1999) A New Class of Repression Modules Is Critical for Heme Regulation of the Yeast Transcriptional Activator Hap1. Molecular and Cellular Biology 19:6, pages 4324-4333.
Read now

Articles from other publishers (47)

Quanli Liu, Yi Liu, Gang Li, Otto Savolainen, Yun Chen & Jens Nielsen. (2021) De novo biosynthesis of bioactive isoflavonoids by engineered yeast cell factories. Nature Communications 12:1.
Crossref
David Schuler, Christina Höll, Nathalie Grün, Jonas Ulrich, Bastian Dillner, Franz Klebl, Alexandra Ammon, Lars M. Voll & Jörg Kämper. (2018) Galactose metabolism and toxicity in Ustilago maydis. Fungal Genetics and Biology 114, pages 42-52.
Crossref
Abhay Kumar, Jaswandi Ujwal Dandekar & Paike Jayadeva Bhat. (2017) Fermentative metabolism impedes p53-dependent apoptosis in a Crabtree-positive but not in Crabtree-negative yeast. Journal of Biosciences 42:4, pages 585-601.
Crossref
Shintaro Ryo, Jun Ishii, Toshihide Matsuno, Yasuyuki Nakamura, Daiki Matsubara, Masahiro Tominaga & Akihiko Kondo. (2017) Positive Feedback Genetic Circuit Incorporating a Constitutively Active Mutant Gal3 into Yeast GAL Induction System. ACS Synthetic Biology 6:6, pages 928-935.
Crossref
Rajesh Kumar Kar, Hungyo Kharerin, Ranjith Padinhateeri & Paike Jayadeva Bhat. (2017) Multiple Conformations of Gal3 Protein Drive the Galactose-Induced Allosteric Activation of the GAL Genetic Switch of Saccharomyces cerevisiae. Journal of Molecular Biology 429:1, pages 158-176.
Crossref
Akshay Kumar Das Adhikari, Mohd. Tanvir Qureshi, Rajesh Kumar Kar & Paike Jayadeva Bhat. (2014) Perturbation of the interaction between Gal4p and Gal80p of the Saccharomyces cerevisiae GAL switch results in altered responses to galactose and glucose . Molecular Microbiology 94:6, pages 1375-1376.
Crossref
Rajesh Kumar Kar, Mohd. Tanvir Qureshi, Akshay Kumar DasAdhikari, Taiyeb Zahir, Kareenhalli V. Venkatesh & Paike Jayadeva Bhat. (2014) Stochastic galactokinase expression underlies GAL gene induction in a GAL3 mutant of Saccharomyces cerevisiae . FEBS Journal 281:7, pages 1798-1817.
Crossref
Pushkar Malakar & Kareenhalli V. Venkatesh. (2014) GAL regulon of Saccharomyces cerevisiae performs optimally to maximize growth on galactose . FEMS Yeast Research 14:2, pages 346-356.
Crossref
Sanjay K. Upadhyay. (2014) Dynamics of Gal80p in the Gal80p–Gal3p complex differ significantly from the dynamics in the Gal80p–Gal1p complex: implications for the higher specificity of Gal3p. Mol. BioSyst. 10:12, pages 3120-3129.
Crossref
Sanjay K. Upadhyay & Yellamraju U. Sasidhar. (2012) Molecular simulation and docking studies of Gal1p and Gal3p proteins in the presence and absence of ligands ATP and galactose: implication for transcriptional activation of GAL genes. Journal of Computer-Aided Molecular Design 26:7, pages 847-864.
Crossref
Tali Lavy, P. Rajesh Kumar, Hongzhen He & Leemor Joshua-Tor. (2012) The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation . Genes & Development 26:3, pages 294-303.
Crossref
Onur Egriboz, Fenglei Jiang & James E Hopper. (2011) Rapid GAL Gene Switch of Saccharomyces cerevisiae Depends on Nuclear Gal3, Not Nucleocytoplasmic Trafficking of Gal3 and Gal80 . Genetics 189:3, pages 825-836.
Crossref
Vikki M. Weake & Jerry L. Workman. (2010) Inducible gene expression: diverse regulatory mechanisms. Nature Reviews Genetics 11:6, pages 426-437.
Crossref
Christopher A. Sellick, Thomas A. Jowitt & Richard J. Reece. (2009) The Effect of Ligand Binding on the Galactokinase Activity of Yeast Gal1p and Its Ability to Activate Transcription. Journal of Biological Chemistry 284:1, pages 229-236.
Crossref
Cuong Q Diep, Xiaorong Tao, Vepkhia Pilauri, Mandy Losiewicz, T Eric Blank & James E Hopper. (2008) Genetic Evidence for Sites of Interaction Between the Gal3 and Gal80 Proteins of the Saccharomyces cerevisiae GAL Gene Switch . Genetics 178:2, pages 725-736.
Crossref
Christopher A. Sellick, Robert N. Campbell & Richard J. Reece. 2008. 111 150 .
Alexander Anders, Hauke Lilie, Kathlen Franke, Lutz Kapp, Jörg Stelling, Ernst D. Gilles & Karin D. Breunig. (2006) The Galactose Switch in Kluyveromyces lactis Depends on Nuclear Competition between Gal4 and Gal1 for Gal80 Binding. Journal of Biological Chemistry 281:39, pages 29337-29348.
Crossref
Ayellet V Segrè, Andrew W Murray & Jun-Yi Leu. (2006) High-Resolution Mutation Mapping Reveals Parallel Experimental Evolution in Yeast. PLoS Biology 4:8, pages e256.
Crossref
Cuong Q Diep, Gang Peng, Maria Bewley, Vepkhia Pilauri, Ira Ropson & James E Hopper. (2006) Intragenic Suppression of Gal3C Interaction With Gal80 in the Saccharomyces cerevisiae GAL Gene Switch . Genetics 172:1, pages 77-87.
Crossref
M RUBIOTEXEIRA. (2005) A comparative analysis of the genetic switch between not-so-distant cousins: versus. FEMS Yeast Research 5:12, pages 1115-1128.
Crossref
James B. Thoden, Christopher A. Sellick, David J. Timson, Richard J. Reece & Hazel M. Holden. (2005) Molecular Structure of Saccharomyces cerevisiae Gal1p, a Bifunctional Galactokinase and Transcriptional Inducer. Journal of Biological Chemistry 280:44, pages 36905-36911.
Crossref
Anirudha Lakshminarasimhan & Paike Jayadeva Bhat. (2005) Replacement of a conserved tyrosine by tryptophan in Gal3p of Saccharomyces cerevisiae reduces constitutive activity: implications for signal transduction in the GAL regulon. Molecular Genetics and Genomics 274:4, pages 384-393.
Crossref
Christopher A. Sellick & Richard J. Reece. (2005) Eukaryotic transcription factors as direct nutrient sensors. Trends in Biochemical Sciences 30:7, pages 405-412.
Crossref
Vepkhia Pilauri, Maria Bewley, Cuong Diep & James Hopper. (2005) Gal80 Dimerization and the Yeast GAL Gene Switch. Genetics 169:4, pages 1903-1914.
Crossref
Anurag Ruhela, Malkhey Verma, Jeremy S. Edwards, P.J. Bhat, Sharad Bhartiya & K.V. Venkatesh. (2004) Autoregulation of regulatory proteins is key for dynamic operation of GAL switch in Saccharomyces cerevisiae . FEBS Letters 576:1-2, pages 119-126.
Crossref
Alinda A. Hasper, Luisa M. Trindade, Douwe van der Veen, Albert J. J. van Ooyen & Leo H. de Graaff. (2004) Functional analysis of the transcriptional activator XlnR from Aspergillus niger. Microbiology 150:5, pages 1367-1375.
Crossref
Malkhey Verma, Paike Jayadeva Bhat & K.V. Venkatesh. (2003) Quantitative Analysis of GAL Genetic Switch of Saccharomyces cerevisiae Reveals That Nucleocytoplasmic Shuttling of Gal80p Results in a Highly Sensitive Response to Galactose. Journal of Biological Chemistry 278:49, pages 48764-48769.
Crossref
R.A. Menezes, C. Amuel, R. Engels, U. Gengenbacher, J. Labahn & C.P. Hollenberg. (2003) Sites for Interaction between Gal80p and Gal1p in Kluyveromyces lactis: Structural Model of Galactokinase based on Homology to the GHMP Protein Family. Journal of Molecular Biology 333:3, pages 479-492.
Crossref
Gene O. Bryant & Mark Ptashne. (2003) Independent Recruitment In Vivo by Gal4 of Two Complexes Required for Transcription. Molecular Cell 11:5, pages 1301-1309.
Crossref
Naoki Kato, Yuriko Murakoshi, Masashi Kato, Tetsuo Kobayashi & Norihiro Tsukagoshi. (2002) Isomaltose formed by α-glucosidases triggers amylase induction in Aspergillus nidulans. Current Genetics 42:1, pages 43-50.
Crossref
Gang Peng & James E. Hopper. (2002) Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proceedings of the National Academy of Sciences 99:13, pages 8548-8553.
Crossref
David J. Timson & Richard J. Reece. (2002) Kinetic analysis of yeast galactokinase: implications for transcriptional activation of the GAL genes. Biochimie 84:4, pages 265-272.
Crossref
P. Jayadeva Bhat & T. V. S. Murthy. (2001) Transcriptional control of the GAL/MEL regulon of yeast Saccharomyces cerevisiae: mechanism of galactose-mediated signal transduction. Molecular Microbiology 40:5, pages 1059-1066.
Crossref
Shelley Ann G. des Etages, Deepti Saxena, Hoching L. Huang, Darlene A. Falvey, Dianna Barber & Marjorie C. Brandriss. (2001) Conformational changes play a role in regulating the activity of the proline utilization pathway-specific regulator in Saccharomyces cerevisiae. Molecular Microbiology 40:4, pages 890-899.
Crossref
Raffael Schaffrath & Karin D. Breunig. (2000) Genetics and Molecular Physiology of the Yeast Kluyveromyces lactis. Fungal Genetics and Biology 30:3, pages 173-190.
Crossref
Michael D'Alessio & Marjorie C. Brandriss. (2000) Cross-Pathway Regulation in Saccharomyces cerevisiae : Activation of the Proline Utilization Pathway by Gal4p In Vivo . Journal of Bacteriology 182:13, pages 3748-3753.
Crossref
Adam Platt, Helen C. Ross, Steven Hankin & Richard J. Reece. (2000) The insertion of two amino acids into a transcriptional inducer converts it into a galactokinase. Proceedings of the National Academy of Sciences 97:7, pages 3154-3159.
Crossref
Alok Kumar Sil, Ping Xin & James E. Hopper. (2000) Vectors Allowing Amplified Expression of the Saccharomyces cerevisiae Gal3p-Gal80p-Gal4p Transcription Switch: Applications to Galactose-Regulated High-Level Production of Proteins. Protein Expression and Purification 18:2, pages 202-212.
Crossref
Zhen Hu, Yingzi Yue, Hua Jiang, Bin Zhang, Peter W Sherwood & Corinne A Michels. (2000) Analysis of the Mechanism by Which Glucose Inhibits Maltose Induction of MAL Gene Expression in Saccharomyces. Genetics 154:1, pages 121-132.
Crossref
R. Bash & D. Lohr. 2000. 197 259 .
Sabire Özcan & Mark Johnston. (1999) Function and Regulation of Yeast Hexose Transporters. Microbiology and Molecular Biology Reviews 63:3, pages 554-569.
Crossref
Ursula Baumgartner, Barbara Hamilton, Martin Piskacek, Helmut Ruis & Hanspeter Rottensteiner. (1999) Functional Analysis of the Zn2Cys6Transcription Factors Oaf1p and Pip2p. Journal of Biological Chemistry 274:32, pages 22208-22216.
Crossref
Dakshesh Vinodrai Mehta, Anaul Kabir & Paike Jayadeva Bhat. (1999) Expression of human inositol monophosphatase suppresses galactose toxicity in Saccharomyces cerevisiae: possible implications in galactosemia. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1454:3, pages 217-226.
Crossref
F.T. Zenke, L. Kapp & K.D. Breunig. (1999) Regulated Phosphorylation of the Gal4p Inhibitor Gal80p of <I>Kluyveromyces lactis</I> Revealed by Mutational Analysis. Biological Chemistry 380:4.
Crossref
Lilyann Novak Frazer. (1998) One stop mycology. Mycological Research 102:10, pages 1277-1296.
Crossref
Richard J. Reece & Adam Platt. (1997) Signaling activation and repression of RNA polymerase II transcription in yeast. BioEssays 19:11, pages 1001-1010.
Crossref
Nandinee Giri & Paike Jayadeva Bhat. (2022) Yeast Galactokinase in Closed Conformation Can Switch between Catalytic and Signal Transducer States. SSRN Electronic Journal.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.