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Molecular and Cellular Biology Volume 18, 1998 - Issue 7
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Gene Expression

RNA Binding Activity of Heterodimeric Splicing Factor U2AF: at Least One RS Domain Is Required for High-Affinity Binding

David Z. RudnerDepartment of Molecular and Cell Biology, University of California, Berkeley, California94720-3204
,
Kevin S. BregerDepartment of Molecular and Cell Biology, University of California, Berkeley, California94720-3204
,
Roland KanaarDepartment of Molecular and Cell Biology, University of California, Berkeley, California94720-3204
,
Melissa D. AdamsDepartment of Molecular and Cell Biology, University of California, Berkeley, California94720-3204
&
Donald C. RioDepartment of Molecular and Cell Biology, University of California, Berkeley, California94720-3204Correspondence[email protected]
Pages 4004-4011 | Received 23 Dec 1997, Accepted 21 Apr 1998, Published online: 28 Mar 2023

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Vinod Sridharan & Ravinder Singh. (2007) A Conditional Role of U2AF in Splicing of Introns with Unconventional Polypyrimidine Tracts. Molecular and Cellular Biology 27:20, pages 7334-7344.
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Rebeccah J. Katzenberger, Matthew S. Marengo & David A. Wassarman. (2006) ATM and ATR Pathways Signal Alternative Splicing of Drosophila TAF1 Pre-mRNA in Response to DNA Damage. Molecular and Cellular Biology 26:24, pages 9256-9267.
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Teresa R. Pacheco, Miguel B. Coelho, Joana M. P. Desterro, Inês Mollet & Maria Carmo-Fonseca. (2006) In Vivo Requirement of the Small Subunit of U2AF for Recognition of a Weak 3′ Splice Site. Molecular and Cellular Biology 26:21, pages 8183-8190.
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Christopher J. Webb & Jo Ann Wise. (2004) The Splicing Factor U2AF Small Subunit Is Functionally Conserved between Fission Yeast and Humans. Molecular and Cellular Biology 24:10, pages 4229-4240.
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Sabine Guth, Concepción Martínez, Rajesh K. Gaur & Juan Valcárcel. (1999) Evidence for Substrate-Specific Requirement of the Splicing Factor U2AF35 and for Its Function after Polypyrimidine Tract Recognition by U2AF65. Molecular and Cellular Biology 19:12, pages 8263-8271.
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Articles from other publishers (21)

Gretter González-Blanco, Guillermina García-Rivera, Patricia Talmás-Rohana, Ester Orozco, José Manuel Galindo-Rosales, Cristina Vélez, Odila Salucedo-Cárdenas, Elisa Azuara-Liceaga, Mario Alberto Rodríguez-Rodríguez, Tomoyoshi Nozaki & Jesús Valdés. (2022) An Unusual U2AF2 Inhibits Splicing and Attenuates the Virulence of the Human Protozoan Parasite Entamoeba histolytica. Frontiers in Cellular and Infection Microbiology 12.
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Laurynas Vilys, Inga Peciuliene, Egle Jakubauskiene, Ruta Zinkeviciute, Yuichi Makino & Arvydas Kanopka. (2021) U2AF - Hypoxia-induced fas alternative splicing regulator. Experimental Cell Research 399:1, pages 112444.
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Tobias Schmidt, Paul Knick, Hauke Lilie, Susann Friedrich, Ralph Peter Golbik & Sven-Erik Behrens. (2017) The properties of the RNA-binding protein NF90 are considerably modulated by complex formation with NF45. Biochemical Journal 474:2, pages 259-280.
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Jean-François Spinella, Pauline Cassart, Chantal Richer, Virginie Saillour, Manon Ouimet, Sylvie Langlois, Pascal St-Onge, Thomas Sontag, Jasmine Healy, Mark D. Minden & Daniel Sinnett. (2016) Genomic characterization of pediatric T-cell acute lymphoblastic leukemia reveals novel recurrent driver mutations. Oncotarget 7:40, pages 65485-65503.
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Chien-Ling Lin, Allison J. Taggart, Kian Huat Lim, Kamil J. Cygan, Luciana Ferraris, Robbert Creton, Yen-Tsung Huang & William G. Fairbrother. (2016) RNA structure replaces the need for U2AF2 in splicing. Genome Research 26:1, pages 12-23.
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Hisashi Yoshida, Sam-Yong Park, Takashi Oda, Taeko Akiyoshi, Mamoru Sato, Mikako Shirouzu, Kengo Tsuda, Kanako Kuwasako, Satoru Unzai, Yutaka Muto, Takeshi Urano & Eiji Obayashi. (2015) A novel 3′ splice site recognition by the two zinc fingers in the U2AF small subunit. Genes & Development 29:15, pages 1649-1660.
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T Okeyo-Owuor, B S White, R Chatrikhi, D R Mohan, S Kim, M Griffith, L Ding, S Ketkar-Kulkarni, J Hundal, K M Laird, C L Kielkopf, T J Ley, M J Walter & T A Graubert. (2014) U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing. Leukemia 29:4, pages 909-917.
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J. Matthew Taliaferro, Nehemiah Alvarez, Richard E. Green, Marco Blanchette & Donald C. Rio. (2011) Evolution of a tissue-specific splicing network. Genes & Development 25:6, pages 608-620.
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Stephanie D. Shaw, Sutapa Chakrabarti, Gourisankar Ghosh & Adrian R. Krainer. (2007) Deletion of the N-terminus of SF2/ASF Permits RS-Domain-Independent Pre-mRNA Splicing. PLoS ONE 2:9, pages e854.
Crossref
Michelle L. Hastings, Eric Allemand, Dominik M. Duelli, Michael P. Myers & Adrian R. Krainer. (2007) Control of Pre-mRNA Splicing by the General Splicing Factors PUF60 and U2AF65. PLoS ONE 2:6, pages e538.
Crossref
Chiara Gamberi, Oona Johnstone & Paul Lasko. 2006. 43 139 .
Kristy L. Henscheid, David S. Shin, S. Craig Cary & J. Andrew Berglund. (2005) The splicing factor U2AF65 is functionally conserved in the thermotolerant deep-sea worm Alvinella pompejana. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1727:3, pages 197-207.
Crossref
Christopher J. Webb, Sujata Lakhe-Reddy, Charles M. Romfo & Jo Ann Wise. (2005) Analysis of Mutant Phenotypes and Splicing Defects Demonstrates Functional Collaboration between the Large and Small Subunits of the Essential Splicing Factor U2AF In Vivo. Molecular Biology of the Cell 16:2, pages 584-596.
Crossref
Brian T. Weinert, Bosun Min & Donald C. Rio. (2005) P element excision and repair by non-homologous end joining occurs in both G1 and G2 of the cell cycle. DNA Repair 4:2, pages 171-181.
Crossref
Helen K Salz, Ricardo S Y Mancebo, Alexis A Nagengast, Olga Speck, Mitchell Psotka & Stephen M Mount. (2004) The Drosophila U1-70K Protein Is Required for Viability, but Its Arginine-Rich Domain Is Dispensable. Genetics 168:4, pages 2059-2065.
Crossref
Clara L. Kielkopf, Stephan Lücke & Michael R. Green. (2004) U2AF homology motifs: protein recognition in the RRM world. Genes & Development 18:13, pages 1513-1526.
Crossref
Marco Blanchette, Emmanuel Labourier, Richard E Green, Steven E Brenner & Donald C Rio. (2004) Genome-Wide Analysis Reveals an Unexpected Function for the Drosophila Splicing Factor U2AF50 in the Nuclear Export of Intronless mRNAs. Molecular Cell 14:6, pages 775-786.
Crossref
Oliver A. Kent, Ayube Reayi, Louise Foong, Kaari A. Chilibeck & Andrew M. MacMillan. (2003) Structuring of the 3′ Splice Site by U2AF65. Journal of Biological Chemistry 278:50, pages 50572-50577.
Crossref
Clara L. Kielkopf, Natalia A. Rodionova, Michael R. Green & Stephen K. Burley. (2001) A Novel Peptide Recognition Mode Revealed by the X-Ray Structure of a Core U2AF35/U2AF65 Heterodimer. Cell 106:5, pages 595-605.
Crossref
Diego A. R. Zorio & Thomas Blumenthal. (1999) Both subunits of U2AF recognize the 3′ splice site in Caenorhabditis elegans. Nature 402:6763, pages 835-838.
Crossref
Margaret A. MacMorris, Diego A. R. Zorio & Thomas Blumenthal. (1999) An exon that prevents transport of a mature mRNA. Proceedings of the National Academy of Sciences 96:7, pages 3813-3818.
Crossref

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