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Molecular and Cellular Biology Volume 19, 1999 - Issue 8
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Transcriptional Regulation

In Vitro Analysis of α-Amanitin-Resistant Transcription from the rRNA, Procyclic Acidic Repetitive Protein, and Variant Surface Glycoprotein Gene Promoters in Trypanosoma brucei

Gabriele LauferAbteilung Zellbiologie, Zoologisches Institut der Universität Tübingen, D-72076Tübingen, Germany
,
Gabriel SchaafAbteilung Zellbiologie, Zoologisches Institut der Universität Tübingen, D-72076Tübingen, Germany
,
Sigrid BollgönnAbteilung Zellbiologie, Zoologisches Institut der Universität Tübingen, D-72076Tübingen, Germany
&
Arthur GünzlAbteilung Zellbiologie, Zoologisches Institut der Universität Tübingen, D-72076Tübingen, GermanyCorrespondence[email protected]
Pages 5466-5473 | Received 03 Mar 1999, Accepted 25 May 1999, Published online: 28 Mar 2023

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Ju Huck Lee, Gang Cai, Aswini K. Panigrahi, Star Dunham-Ems, Tu N. Nguyen, Justin D. Radolf, Francisco J. Asturias & Arthur Günzl. (2010) A TFIIH-Associated Mediator Head Is a Basal Factor of Small Nuclear Spliced Leader RNA Gene Transcription in Early-Diverged Trypanosomes. Molecular and Cellular Biology 30:23, pages 5502-5513.
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Tu N. Nguyen, Bernd Schimanski & Arthur Günzl. (2007) Active RNA Polymerase I of Trypanosoma brucei Harbors a Novel Subunit Essential for Transcription. Molecular and Cellular Biology 27:17, pages 6254-6263.
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Bernd Schimanski, Tu N. Nguyen & Arthur Günzl. (2005) Characterization of a Multisubunit Transcription Factor Complex Essential for Spliced-Leader RNA Gene Transcription in Trypanosoma brucei. Molecular and Cellular Biology 25:16, pages 7303-7313.
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Jia-peng Ruan, George K. Arhin, Elisabetta Ullu & Christian Tschudi. (2004) Functional Characterization of a Trypanosoma brucei TATA-Binding Protein-Related Factor Points to a Universal Regulator of Transcription in Trypanosomes. Molecular and Cellular Biology 24:21, pages 9610-9618.
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Articles from other publishers (45)

Ankita Srivastava, Daniela L Ambrósio, Monika Tasak, Ujwala Gosavi & Arthur Günzl. (2021) A distinct complex of PRP19-related and trypanosomatid-specific proteins is required for pre-mRNA splicing in trypanosomes. Nucleic Acids Research 49:22, pages 12929-12942.
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Vanessa Luzak, Lara López-EscobarT. Nicolai Siegel & Luisa M. Figueiredo. (2021) Cell-to-Cell Heterogeneity in Trypanosomes. Annual Review of Microbiology 75:1, pages 107-128.
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Ujwala Gosavi, Ankita Srivastava, Nitika Badjatia & Arthur Günzl. (2020) Rapid block of pre‐mRNA splicing by chemical inhibition of analog‐sensitive CRK9 in Trypanosoma brucei . Molecular Microbiology 113:6, pages 1225-1239.
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Arthur Günzl, Ankita Srivastava & Ujwala Gosavi. 2020. Trypanosomatids. Trypanosomatids 265 284 .
Santiago Martínez-Calvillo, Luis E. Florencio-Martínez & Tomás Nepomuceno-Mejía. (2019) Nucleolar Structure and Function in Trypanosomatid Protozoa. Cells 8:5, pages 421.
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Ankita Srivastava, Nitika Badjatia, Ju Huck Lee, Bing Hao & Arthur Günzl. (2018) An RNA polymerase II-associated TFIIF-like complex is indispensable for SL RNA gene transcription in Trypanosoma brucei. Nucleic Acids Research 46:4, pages 1695-1709.
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Gabriela Romero‐Meza, Daniel E. Vélez‐Ramírez, Luis E. Florencio‐Martínez, Fiordaliso C. Román‐Carraro, Rebeca Manning‐Cela, Rosaura Hernández‐Rivas & Santiago Martínez‐Calvillo. (2016) M af1 is a negative regulator of transcription in T rypanosoma brucei . Molecular Microbiology 103:3, pages 452-468.
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Daniela L. Ambrósio, Nitika Badjatia & Arthur Günzl. (2015) The spliceosomal PRP19 complex of trypanosomes. Molecular Microbiology 95:5, pages 885-901.
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Roberto Hernández & Ana María Cevallos. (2014) Ribosomal RNA gene transcription in trypanosomes. Parasitology Research 113:7, pages 2415-2424.
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Tu N. Nguyen, Laura S. M. Müller, Sung Hee Park, T. Nicolai Siegel & Arthur Günzl. (2014) Promoter occupancy of the basal class I transcription factor A differs strongly between active and silent VSG expression sites in Trypanosoma brucei. Nucleic Acids Research 42:5, pages 3164-3176.
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Nitika Badjatia, Tu N. Nguyen, Ju Huck Lee & Arthur Günzl. (2013) Trypanosoma brucei harbours a divergent XPB helicase paralogue that is specialized in nucleotide excision repair and conserved among kinetoplastid organisms . Molecular Microbiology 90:6, pages 1293-1308.
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Tu N. Nguyen, Bao N. Nguyen, Ju Huck Lee, Aswini K. Panigrahi & Arthur Günzl. (2012) Characterization of a Novel Class I Transcription Factor A (CITFA) Subunit That Is Indispensable for Transcription by the Multifunctional RNA Polymerase I of Trypanosoma brucei. Eukaryotic Cell 11:12, pages 1573-1581.
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Megan L. Povelones, Eva Gluenz, Marcin Dembek, Keith Gull & Gloria Rudenko. (2012) Histone H1 Plays a Role in Heterochromatin Formation and VSG Expression Site Silencing in Trypanosoma brucei. PLoS Pathogens 8:11, pages e1003010.
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Kellie Whitecavage Solnoki, Allison H. Sing, Caitlyn J. Sofa, Russell Miller, Paulina A. Ogorzalek, Hillary V. Penek & Jennifer B. Palenchar. (2012) TbENF is an essential TbTFIIB-interacting trypanosomatid-specific factor. Molecular and Biochemical Parasitology 181:2, pages 94-101.
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Sung Hee Park, Tu N. Nguyen & Arthur Günzl. (2012) Development of an efficient in vitro transcription system for bloodstream form Trypanosoma brucei reveals life cycle-independent functionality of class I transcription factor A. Molecular and Biochemical Parasitology 181:1, pages 29-36.
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Sung Hee Park, Tu N. Nguyen, Justin K. Kirkham, Ju Huck Lee & Arthur Günzl. (2011) Transcription by the multifunctional RNA polymerase I in Trypanosoma brucei functions independently of RPB7. Molecular and Biochemical Parasitology 180:1, pages 35-42.
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Arthur Günzl. (2010) The Pre-mRNA Splicing Machinery of Trypanosomes: Complex or Simplified?. Eukaryotic Cell 9:8, pages 1159-1170.
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Hadassa Shaked, Chaim Wachtel, Pawel Tulinski, Nasreen Hag Yahia, Omer Barda, Edward Darzynkiewicz, Timothy W. Nilsen & Shulamit Michaeli. (2010) Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA. Nucleic Acids Research 38:10, pages e114-e114.
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Santiago Martínez-Calvillo, Juan C. Vizuet-de-Rueda, Luis E. Florencio-Martínez, Rebeca G. Manning-Cela & Elisa E. Figueroa-Angulo. (2010) Gene Expression in Trypanosomatid Parasites. Journal of Biomedicine and Biotechnology 2010, pages 1-15.
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Henry K. Bayele. (2009) Trypanosoma brucei: A putative RNA polymerase II promoter. Experimental Parasitology 123:4, pages 313-318.
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Daniela Luz Ambrósio, Ju Huck Lee, Aswini K. Panigrahi, Tu Ngoc Nguyen, Regina Maria Barretto Cicarelli & Arthur Günzl. (2009) Spliceosomal Proteomics in Trypanosoma brucei Reveal New RNA Splicing Factors . Eukaryotic Cell 8:7, pages 990-1000.
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Ju Huck Lee, Hyun Suk Jung & Arthur Günzl. (2009) Transcriptionally active TFIIH of the early-diverged eukaryote Trypanosoma brucei harbors two novel core subunits but not a cyclin-activating kinase complex. Nucleic Acids Research 37:11, pages 3811-3820.
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Xenia Peñate, Diana López‐Farfán, David Landeira, Amy Wentland, Isabel Vidal & Miguel Navarro. (2009) RNA pol II subunit RPB7 is required for RNA pol I‐mediated transcription in Trypanosoma brucei . EMBO reports 10:3, pages 252-257.
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Jens Brandenburg, Bernd Schimanski, Everson Nogoceke, Tu N Nguyen, Júlio C Padovan, Brian T Chait, George A M Cross & Arthur Günzl. (2007) Multifunctional class I transcription in Trypanosoma brucei depends on a novel protein complex. The EMBO Journal 26:23, pages 4856-4866.
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Peter Burton, David J. McBride, Jonathan M. Wilkes, J. David Barry & Richard McCulloch. (2007) Ku Heterodimer-Independent End Joining in Trypanosoma brucei Cell Extracts Relies upon Sequence Microhomology . Eukaryotic Cell 6:10, pages 1773-1781.
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Ju Huck Lee, Tu N. Nguyen, Bernd Schimanski & Arthur Günzl. (2007) Spliced Leader RNA Gene Transcription in Trypanosoma brucei Requires Transcription Factor TFIIH . Eukaryotic Cell 6:4, pages 641-649.
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Elisa Figueroa-Angulo, Ana María Cevallos, Alejandro Zentella, Imelda López-Villaseñor & Roberto Hernández. (2006) Potential regulatory elements in the Trypanosoma cruzi rRNA gene promoter. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression 1759:10, pages 497-501.
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Tu N. Nguyen, Bernd Schimanski, André Zahn, Birgit Klumpp & Arthur Günzl. (2006) Purification of an eight subunit RNA polymerase I complex in Trypanosoma brucei. Molecular and Biochemical Parasitology 149:1, pages 27-37.
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Bernd Schimanski, Tu N. Nguyen & Arthur Günzl. (2005) Highly Efficient Tandem Affinity Purification of Trypanosome Protein Complexes Based on a Novel Epitope Combination. Eukaryotic Cell 4:11, pages 1942-1950.
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Bernd Schimanski, Luyan Ma & Arthur Günzl. (2004) Failure to detect binding of Trypanosoma brucei SNAPc to U2 and U6 snRNA gene sequences by in vitro transcription competition and pull-down assays. Molecular and Biochemical Parasitology 137:2, pages 293-296.
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Arthur Günzl, Thomas Bruderer, Gabriele Laufer, Bernd Schimanski, Lan-Chun Tu, Hui-Min Chung, Pei-Tseng Lee & Mary Gwo-Shu Lee. (2003) RNA Polymerase I Transcribes Procyclin Genes and Variant Surface Glycoprotein Gene Expression Sites in Trypanosoma brucei . Eukaryotic Cell 2:3, pages 542-551.
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Bernd Schimanski, Birgit Klumpp, Gabriele Laufer, Richard J. Marhöfer, Paul M. Selzer & Arthur Günzl. (2003) The second largest subunit of Trypanosoma brucei’s multifunctional RNA polymerase I has a unique N-terminal extension domain. Molecular and Biochemical Parasitology 126:2, pages 193-200.
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Matthew Berriman, Neil Hall, Karen Sheader, Frédéric Bringaud, Bela Tiwari, Tomoko Isobe, Sharen Bowman, Craig Corton, Louise Clark, George A.M Cross, Maarten Hoek, Tyiesha Zanders, Magali Berberof, Piet Borst & Gloria Rudenko. (2002) The architecture of variant surface glycoprotein gene expression sites in Trypanosoma brucei. Molecular and Biochemical Parasitology 122:2, pages 131-140.
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Miguel Navarro & Keith Gull. (2001) A pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei. Nature 414:6865, pages 759-763.
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Maarten Hoek & George A.M Cross. (2001) Expression-site-associated-gene-8 (ESAG8) is not required for regulation of the VSG expression site in Trypanosoma brucei. Molecular and Biochemical Parasitology 117:2, pages 211-215.
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Gabriele Laufer & Arthur Günzl. (2001) In-vitro competition analysis of procyclin gene and variant surface glycoprotein gene expression site transcription in Trypanosoma brucei. Molecular and Biochemical Parasitology 113:1, pages 55-65.
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