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Molecular and Cellular Biology Volume 22, 2002 - Issue 22
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DNA Dynamics and Chromosome Structure

A RAG-1/RAG-2 Tetramer Supports 12/23-Regulated Synapsis, Cleavage, and Transposition of V(D)J Recombination Signals

Patrick C. SwansonDepartment of Medical Microbiology and Immunology, School of Medicine, Creighton University, Omaha, Nebraska68178Correspondence[email protected]
Pages 7790-7801 | Received 18 Jul 2002, Accepted 21 Aug 2002, Published online: 28 Mar 2023

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Keerthi Shetty & David G. Schatz. (2015) Recruitment of RAG1 and RAG2 to Chromatinized DNA during V(D)J Recombination. Molecular and Cellular Biology 35:21, pages 3701-3713.
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Joydeep K. Banerjee & David G. Schatz. (2014) Synapsis Alters RAG-Mediated Nicking at Tcrb Recombination Signal Sequences: Implications for the “Beyond 12/23” Rule. Molecular and Cellular Biology 34:14, pages 2566-2580.
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Noriko Shimazaki, Amjad Askary, Patrick C. Swanson & Michael R. Lieber. (2012) Mechanistic Basis for RAG Discrimination between Recombination Sites and the Off-Target Sites of Human Lymphomas. Molecular and Cellular Biology 32:2, pages 365-375.
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Julien Bischerour, Catherine Lu, David B. Roth & Ronald Chalmers. (2009) Base Flipping in V(D)J Recombination: Insights into the Mechanism of Hairpin Formation, the 12/23 Rule, and the Coordination of Double-Strand Breaks. Molecular and Cellular Biology 29:21, pages 5889-5899.
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Mihai Ciubotaru, Aleksei N. Kriatchko, Patrick C. Swanson, Frank V. Bright & David G. Schatz. (2007) Fluorescence Resonance Energy Transfer Analysis of Recombination Signal Sequence Configuration in the RAG1/2 Synaptic Complex. Molecular and Cellular Biology 27:13, pages 4745-4758.
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Aleksei N. Kriatchko, Dirk K. Anderson & Patrick C. Swanson. (2006) Identification and Characterization of a Gain-of-Function RAG-1 Mutant. Molecular and Cellular Biology 26:12, pages 4712-4728.
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Yan Dai, Ben Wong, Yi-Meng Yen, Marjorie A. Oettinger, Jongbum Kwon & Reid C. Johnson. (2005) Determinants of HMGB Proteins Required To Promote RAG1/2-Recombination Signal Sequence Complex Assembly and Catalysis during V(D)J Recombination. Molecular and Cellular Biology 25:11, pages 4413-4425.
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Mihai Ciubotaru & David G. Schatz. (2004) Synapsis of Recombination Signal Sequences Located in cis and DNA Underwinding in V(D)J Recombination. Molecular and Cellular Biology 24:19, pages 8727-8744.
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Karen Lipkow, Nicolas Buisine, David J. Lampe & Ronald Chalmers. (2004) Early Intermediates of mariner Transposition: Catalysis without Synapsis of the Transposon Ends Suggests a Novel Architecture of the Synaptic Complex. Molecular and Cellular Biology 24:18, pages 8301-8311.
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Pallabi De, Mandy M. Peak & Karla K. Rodgers. (2004) DNA Cleavage Activity of the V(D)J Recombination Protein RAG1 Is Autoregulated. Molecular and Cellular Biology 24:15, pages 6850-6860.
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Articles from other publishers (53)

Walker Hoolehan, Justin C Harris, Jennifer N Byrum, Destiny A Simpson & Karla K Rodgers. (2022) An updated definition of V(D)J recombination signal sequences revealed by high-throughput recombination assays. Nucleic Acids Research 50:20, pages 11696-11711.
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Ying Wang, Jun Liu, Peter D. Burrows & Ji-Yang Wang. 2020. B Cells in Immunity and Tolerance. B Cells in Immunity and Tolerance 1 22 .
Christopher M. Kirkham, James N.F. Scott, Xiaoling Wang, Alastair L. Smith, Adam P. Kupinski, Anthony M. Ford, David R. Westhead, Peter G. Stockley, Roman Tuma & Joan Boyes. (2019) Cut-and-Run: A Distinct Mechanism by which V(D)J Recombination Causes Genome Instability. Molecular Cell 74:3, pages 584-597.e9.
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John BettridgeChan Hyun Na, Akhilesh Pandey & Stephen Desiderio. (2017) H3K4me3 induces allosteric conformational changes in the DNA-binding and catalytic regions of the V(D)J recombinase. Proceedings of the National Academy of Sciences 114:8, pages 1904-1909.
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Jennifer ZagelbaumNoriko Shimazaki, Zitadel Anne Esguerra, Go Watanabe, Michael R. Lieber & Eli Rothenberg. (2016) Real-time analysis of RAG complex activity in V(D)J recombination. Proceedings of the National Academy of Sciences 113:42, pages 11853-11858.
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A Troshchynsky, I Dzneladze, L Chen, Y Sheng, V Saridakis & G E Wu. (2015) Functional analyses of polymorphic variants of human terminal deoxynucleotidyl transferase. Genes & Immunity 16:6, pages 388-398.
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Mikalai Lapkouski, Watchalee Chuenchor, Min-Sung Kim, Martin Gellert & Wei Yang. (2015) Assembly Pathway and Characterization of the RAG1/2-DNA Paired and Signal-end Complexes. Journal of Biological Chemistry 290:23, pages 14618-14625.
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Jennifer N. Byrum, Shuying Zhao, Negar S. Rahman, Lori M. Gwyn, William Rodgers & Karla K. Rodgers. (2015) An interdomain boundary in RAG1 facilitates cooperative binding to RAG2 in formation of the V(D)J recombinase complex. Protein Science 24:5, pages 861-873.
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Geoffrey A. Lovely, Robert C. Brewster, David G. Schatz, David Baltimore & Rob Phillips. (2015) Single-molecule analysis of RAG-mediated V(D)J DNA cleavage. Proceedings of the National Academy of Sciences 112:14.
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Min-Sung Kim, Mikalai Lapkouski, Wei Yang & Martin Gellert. (2015) Crystal structure of the V(D)J recombinase RAG1–RAG2. Nature 518:7540, pages 507-511.
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Mihai Ciubotaru, Marius D. Surleac, Lauren Ann Metskas, Peter Koo, Elizabeth Rhoades, Andrei J. Petrescu & David G. Schatz. (2015) The architecture of the 12RSS in V(D)J recombination signal and synaptic complexes. Nucleic Acids Research 43:2, pages 917-931.
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Alicia J. Little, Adam Matthews, Marjorie Oettinger, David B. Roth & David G. Schatz. 2015. Molecular Biology of B Cells. Molecular Biology of B Cells 13 34 .
Rui Kang, Ruochan Chen, Qiuhong Zhang, Wen Hou, Sha Wu, Lizhi Cao, Jin Huang, Yan Yu, Xue-gong Fan, Zhengwen Yan, Xiaofang Sun, Haichao Wang, Qingde Wang, Allan Tsung, Timothy R. Billiar, Herbert J. ZehIIIIII, Michael T. Lotze & Daolin Tang. (2014) HMGB1 in health and disease. Molecular Aspects of Medicine 40, pages 1-116.
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Alicia J. Little, Elizabeth Corbett, Fabian Ortega & David G. Schatz. (2013) Cooperative recruitment of HMGB1 during V(D)J recombination through interactions with RAG1 and DNA. Nucleic Acids Research 41:5, pages 3289-3301.
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Mihai Ciubotaru, Adam J. Trexler, Laurentiu N. Spiridon, Marius D. Surleac, Elizabeth Rhoades, Andrei J. Petrescu & David G. Schatz. (2013) RAG and HMGB1 create a large bend in the 23RSS in the V(D)J recombination synaptic complexes. Nucleic Acids Research 41:4, pages 2437-2454.
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Ming-Guang Mao, Ji-Lin Lei, Perálvarez-Marín Alex, Wan-Shu Hong & Ke-Jian Wang. (2012) Characterization of RAG1 and IgM (mu chain) marking development of the immune system in red-spotted grouper (Epinephelus akaara). Fish & Shellfish Immunology 33:4, pages 725-735.
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Guannan Wang, Kajari Dhar, Patrick C. Swanson, Marcia Levitus & Yung Chang. (2012) Real-time monitoring of RAG-catalyzed DNA cleavage unveils dynamic changes in coding end association with the coding end complex. Nucleic Acids Research 40:13, pages 6082-6096.
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David G. SchatzPatrick C. Swanson. (2011) V(D)J Recombination: Mechanisms of Initiation. Annual Review of Genetics 45:1, pages 167-202.
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Masashi Numata & Kyosuke Nagata. (2011) Synergistic requirement of orphan nonamer-like elements and DNA bending enhanced by HMGB1 for RAG-mediated nicking at cryptic 12-RSS but not authentic 12-RSS. Genes to Cells 16:8, pages 879-895.
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E. V. Chikhirzhina, A. M. Polyanichko, E. I. Kostyleva & V. I. Vorobyev. (2011) Structure of DNA complexes with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions: 1. Circular dichroism spectroscopy. Molecular Biology 45:2, pages 318-326.
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Yi-Hong Chen, Xiao-Ting Jia, Xian-De Huang, Shuang Zhang, Mei Li, Jun-Feng Xie, Shao-Ping Weng & Jian-Guo He. (2011) Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT and LvDorsal to activate the promoter of white spot syndrome virus immediate-early gene ie1. Molecular Immunology 48:5, pages 793-799.
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Stephen Desiderio. (2010) Temporal and spatial regulatory functions of the V(D)J recombinase. Seminars in Immunology 22:6, pages 362-369.
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Luda S. Shlyakhtenko, Jamie Gilmore, Aleksei N. Kriatchko, Sushil Kumar, Patrick C. Swanson & Yuri L. Lyubchenko. (2009) Molecular Mechanism Underlying RAG1/RAG2 Synaptic Complex Formation. Journal of Biological Chemistry 284:31, pages 20956-20965.
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Gabrielle J. Grundy, Santiago Ramón-Maiques, Emilios K. Dimitriadis, Svetlana Kotova, Christian Biertümpfel, J. Bernard Heymann, Alasdair C. Steven, Martin Gellert & Wei Yang. (2009) Initial Stages of V(D)J Recombination: The Organization of RAG1/2 and RSS DNA in the Postcleavage Complex. Molecular Cell 35:2, pages 217-227.
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Fang Fang Yin, Scott Bailey, C Axel Innis, Mihai Ciubotaru, Satwik Kamtekar, Thomas A Steitz & David G Schatz. (2009) Structure of the RAG1 nonamer binding domain with DNA reveals a dimer that mediates DNA synapsis. Nature Structural & Molecular Biology 16:5, pages 499-508.
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Sushil Kumar & Patrick C. Swanson. (2009) Full-length RAG1 promotes contact with coding and intersignal sequences in RAG protein complexes bound to recombination signals paired in cis. Nucleic Acids Research 37:7, pages 2211-2226.
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Patrick C. Swanson, Sushil Kumar & Prafulla Raval. 2009. V(D)J Recombination. V(D)J Recombination 1 15 .
Pallabi De, Shuying Zhao, Lori M Gwyn, LeAnn J Godderz, Mandy M Peak & Karla K Rodgers. (2008) Thermal dependency of RAG1 self-association properties. BMC Biochemistry 9:1.
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Prafulla Raval, Aleksei N. Kriatchko, Sushil Kumar & Patrick C. Swanson. (2008) Evidence for Ku70/Ku80 association with full-length RAG1. Nucleic Acids Research 36:6, pages 2060-2072.
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