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Gene Expression

Spatial Organization of Protein-RNA Interactions in the Branch Site-3′ Splice Site Region during pre-mRNA Splicing in Yeast

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Pages 4174-4186 | Received 06 Nov 2002, Accepted 18 Mar 2003, Published online: 27 Mar 2023

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Read on this site (7)

Nan-Ying Wu, Che-Sheng Chung & Soo-Chen Cheng. (2017) Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5′ Splice Site Selection. Molecular and Cellular Biology 37:6.
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Ting-Wei Chiang & Soo-Chen Cheng. (2013) A Weak Spliceosome-Binding Domain of Yju2 Functions in the First Step and Bypasses Prp16 in the Second Step of Splicing. Molecular and Cellular Biology 33:9, pages 1746-1755.
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Olivier Cordin & Jean D. Beggs. (2013) RNA helicases in splicing. RNA Biology 10:1, pages 83-95.
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Hsueh-Lien Liu & Soo-Chen Cheng. (2012) The Interaction of Prp2 with a Defined Region of the Intron Is Required for the First Splicing Reaction. Molecular and Cellular Biology 32:24, pages 5056-5066.
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Tzu-Chi Yeh, Hseuh-Lien Liu, Che-Sheng Chung, Nan-Ying Wu, Yen-Chi Liu & Soo-Chen Cheng. (2011) Splicing Factor Cwc22 Is Required for the Function of Prp2 and for the Spliceosome To Escape from a Futile Pathway. Molecular and Cellular Biology 31:1, pages 43-53.
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Qiang Wang, Jin He, Bert Lynn & Brian C. Rymond. (2005) Interactions of the Yeast SF3b Splicing Factor. Molecular and Cellular Biology 25:24, pages 10745-10754.
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Dagmar Bačíková & David S. Horowitz. (2005) Genetic and Functional Interaction of Evolutionarily Conserved Regions of the Prp18 Protein and the U5 snRNA. Molecular and Cellular Biology 25:6, pages 2107-2116.
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Articles from other publishers (54)

Corbin S. Black, Thomas A. Whelan, Erin L. Garside, Andrew M. MacMillan, Naomi M. Fast & Stephen D. Rader. (2023) Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes. RNA 29:5, pages 531-550.
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Lisa M. Strittmatter, Charlotte Capitanchik, Andrew J. Newman, Martina Hallegger, Christine M. Norman, Sebastian M. Fica, Chris Oubridge, Nicholas M. Luscombe, Jernej Ule & Kiyoshi Nagai. (2021) psiCLIP reveals dynamic RNA binding by DEAH-box helicases before and after exon ligation. Nature Communications 12:1.
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Max E. WilkinsonClément CharentonKiyoshi Nagai. (2020) RNA Splicing by the Spliceosome. Annual Review of Biochemistry 89:1, pages 359-388.
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Xuhua Xia. (2020) RNA-Seq approach for accurate characterization of splicing efficiency of yeast introns. Methods 176, pages 25-33.
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Nan-Ying Wu & Soo-Chen Cheng. (2019) Functional analysis of Cwc24 ZF-domain in 5′ splice site selection. Nucleic Acids Research 47:19, pages 10327-10339.
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Che-Sheng Chung, Chi-Kang Tseng, Yung-Hua Lai, Hui-Fang Wang, Andrew J Newman & Soo-Chen Cheng. (2019) Dynamic protein–RNA interactions in mediating splicing catalysis. Nucleic Acids Research 47:2, pages 899-910.
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Tucker J. Carrocci, Joshua C. Paulson & Aaron A. Hoskins. (2018) Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex. RNA 24:8, pages 1028-1040.
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Yigong Shi. (2017) Mechanistic insights into precursor messenger RNA splicing by the spliceosome. Nature Reviews Molecular Cell Biology 18:11, pages 655-670.
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Megan Mayerle & Christine Guthrie. (2017) Genetics and biochemistry remain essential in the structural era of the spliceosome. Methods 125, pages 3-9.
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Yigong Shi. (2017) The Spliceosome: A Protein-Directed Metalloribozyme. Journal of Molecular Biology 429:17, pages 2640-2653.
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Jean-Baptiste Fourmann, Marcel J. Tauchert, Ralf Ficner, Patrizia Fabrizio & Reinhard Lührmann. (2017) Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2. Nucleic Acids Research 45:7, pages 4068-4080.
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Tucker J. Carrocci, Douglas M. Zoerner, Joshua C. Paulson & Aaron A. Hoskins. (2017) SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. Nucleic Acids Research, pages gkw1349.
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Qing Tang, Susana Rodriguez-Santiago, Jing Wang, Jia Pu, Andrea Yuste, Varun Gupta, Alberto Moldón, Yong-Zhen Xu & Charles C. Query. (2017) SF3B1/Hsh155 HEAT motif mutations affect interaction with the spliceosomal ATPase Prp5, resulting in altered branch site selectivity in pre-mRNA splicing. Genes & Development 30:24, pages 2710-2723.
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R. Rauhut, P. Fabrizio, O. Dybkov, K. Hartmuth, V. Pena, A. Chari, V. Kumar, C.-T. Lee, H. Urlaub, B. Kastner, H. Stark & R. Luhrmann. (2016) Molecular architecture of the Saccharomyces cerevisiae activated spliceosome. Science 353:6306, pages 1399-1405.
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Chengfu Sun, Norbert Rigo, Patrizia Fabrizio, Berthold Kastner & Reinhard Lührmann. (2016) A protein map of the yeast activated spliceosome as obtained by electron microscopy. RNA 22:9, pages 1427-1440.
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Ruixue WanChuangye YanRui BaiGaoxingyu HuangYigong Shi. (2016) Structure of a yeast catalytic step I spliceosome at 3.4 Å resolution. Science 353:6302, pages 895-904.
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Inessa De, Jana Schmitzová & Vladimir Pena. (2016) The organization and contribution of helicases to RNA splicing . WIREs RNA 7:2, pages 259-274.
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William Perea, Kersten T. Schroeder, Amy N. Bryant & Nancy L. Greenbaum. (2016) Interaction between the Spliceosomal Pre-mRNA Branch Site and U2 snRNP Protein p14. Biochemistry 55:4, pages 629-632.
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Daniel R. Semlow, Mario R. Blanco, Nils G. Walter & Jonathan P. Staley. (2016) Spliceosomal DEAH-Box ATPases Remodel Pre-mRNA to Activate Alternative Splice Sites. Cell 164:5, pages 985-998.
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Norbert Rigo, Chengfu Sun, Patrizia Fabrizio, Berthold Kastner & Reinhard Lührmann. (2015) Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex. The EMBO Journal 34:24, pages 3059-3073.
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Yen-Chi Liu & Soo-Chen Cheng. (2015) Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing. Journal of Biomedical Science 22:1.
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Cornelius Schneider, Dmitry E. Agafonov, Jana Schmitzová, Klaus Hartmuth, Patrizia Fabrizio & Reinhard Lührmann. (2015) Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes. PLOS Genetics 11:9, pages e1005539.
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Wen-Wei Liang & Soo-Chen Cheng. (2015) A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence. Genes & Development 29:1, pages 81-93.
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Zbigniew Warkocki, Cornelius Schneider, Sina Mozaffari-Jovin, Jana Schmitzová, Claudia Höbartner, Patrizia Fabrizio & Reinhard Lührmann. (2015) The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome. Genes & Development 29:1, pages 94-107.
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Piotr Wysoczański, Cornelius Schneider, ShengQi Xiang, Francesca Munari, Simon Trowitzsch, Markus C Wahl, Reinhard Lührmann, Stefan Becker & Markus Zweckstetter. (2014) Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Nature Structural & Molecular Biology 21:10, pages 911-918.
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Daniel J. Crawford, Aaron A. Hoskins, Larry J. Friedman, Jeff Gelles & Melissa J. Moore. (2013) Single-molecule colocalization FRET evidence that spliceosome activation precedes stable approach of 5′ splice site and branch site. Proceedings of the National Academy of Sciences 110:17, pages 6783-6788.
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