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Bioscience, Biotechnology, and Biochemistry Volume 66, 2002 - Issue 7
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Original Articles

A Highly Sensitive Assay for Proteases Using Staphylococcal Protein A Fused with Enhanced Green Fluorescent Protein

Hiroyoshi FUJINODepartment of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido Ishikari-Tobetsu, Hokkaido 061-0293, Japan;Katayama Chemical Industries Co. 3-26-22 Higashi-Nanba, Amagasaki 660-0892, Japan
,
Takashi AOKIDepartment of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido Ishikari-Tobetsu, Hokkaido 061-0293, Japan
&
Hiroyuki WATABEDepartment of Biochemistry, Faculty of Pharmaceutical Sciences, Health Sciences University of Hokkaido Ishikari-Tobetsu, Hokkaido 061-0293, Japan
Pages 1601-1604 | Received 28 Jan 2002, Accepted 26 Feb 2002, Published online: 22 May 2014

Citations (6)

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Shirou TSUCHIDA, Takashi SHIGEMATSU, Taemi YAHARA, Kana NUNOME, Naoya HAMAUE & Takashi AOKI. (2010) Application of “Homogeneous Assay for Fluorescence Concentrated on Membrane” to the Analysis of the Substrate Specificity of Protease. Bioscience, Biotechnology, and Biochemistry 74:4, pages 869-871.
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Articles from other publishers (5)

Xiao-Tian Yu, Xiao-Yan Fu, Xiao-Yi Gao, Xiao-Kun Zhang, Shu-Juan Liang, Hong-Ming Yang & Jin-Bao Tang. (2020) Fc-specific and covalent conjugation of a fluorescent protein to a native antibody through a photoconjugation strategy for fabrication of a novel photostable fluorescent antibody. Analytical and Bioanalytical Chemistry 413:3, pages 945-953.
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Hong-Ming Yang, Yong Chen, Zhi-Qin Gao & Jin-Bao Tang. (2011) Preparation of a bio-immunoreagent between ZZ affibody and enhanced green fluorescent protein for immunofluorescence applications. World Journal of Microbiology and Biotechnology 28:3, pages 1281-1285.
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Jin-bao Tang, Peng Zhu, Hong-ming Yang, Li-min Sun, Shu-liang Song & Ai-guo Ji. (2008) Expression and secretion of recombinant ZZ–EGFP fusion protein by the methylotrophic yeast Pichia pastoris. Biotechnology Letters 30:8, pages 1409-1414.
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Takashi Aoki, Shirou Tsuchida, Taemi Yahara & Naoya Hamaue. (2008) Novel assays for proteases using green fluorescent protein-tagged substrate immobilized on a membrane disk. Analytical Biochemistry 378:2, pages 132-137.
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Chandran Sandhya, Alagarsamy Sumantha & Ashok Pandey. 2006. Enzyme Technology. Enzyme Technology 319 332 .

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