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Bioscience, Biotechnology, and Biochemistry Volume 71, 2007 - Issue 12
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Original Articles

A New Method for the Modification of Fibroin Heavy Chain Protein in the Transgenic Silkworm

Katsura KOJIMANational Institute of Agrobiological Sciences
,
Yoshihiko KUWANANational Institute of Agrobiological Sciences
,
Hideki SEZUTSUNational Institute of Agrobiological Sciences
,
Isao KOBAYASHINational Institute of Agrobiological Sciences
,
Keiro UCHINONational Institute of Agrobiological Sciences
,
Toshiki TAMURANational Institute of Agrobiological Sciences
&
Yasushi TAMADANational Institute of Agrobiological Sciences
 show all
Pages 2943-2951 | Received 05 Jun 2007, Accepted 22 Aug 2007, Published online: 22 May 2014

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Zhenghua Zhu, Yuka Kikuchi, Katsura Kojima, Toshiki Tamura, Nobuo Kuwabara, Takashi Nakamura & Tetsuo Asakura. (2010) Mechanical Properties of Regenerated Bombyx mori Silk Fibers and Recombinant Silk Fibers Produced by Transgenic Silkworms. Journal of Biomaterials Science, Polymer Edition 21:3, pages 395-411.
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Articles from other publishers (55)

Pornanong Aramwit, Qiuran Jiang, Supritha Muppuri & Narendra Reddy. (2023) Transgenic modifications of silkworms as a means to obtain therapeutic biomolecules and protein fibers with exceptional properties. Biotechnology and Bioengineering.
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Nobuto YAMADA, Yoshiko MISE, Naoyuki YONEMURA, Hiroki SAKAI, Keiro UCHINO, Hideki SEZUTSU, Toshiki TAMURA & Tetsuya IIZUKA. (2023) Development of an Injection Method for the Genetic Engineering of Diapause Silkworm Egg Using Dimethyl Sulfoxide. Japan Agricultural Research Quarterly: JARQ 57:1, pages 63-72.
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Hideki Sezutsu & Toshiki Tamura. 2022. Transgenic Insects. Transgenic Insects 395 415 .
Khasru Alam, V. S. Raviraj, Tanmay Chowdhury, Anil Bhuimali, Parthadeb Ghosh & Soumen Saha. (2021) Application of biotechnology in sericulture: Progress, scope and prospect. The Nucleus 65:1, pages 129-150.
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YUSUKE KAMBE. (2022) Functionalization of Silk Fibroin as a Scaffold for Tissue Engineering and Regenerative Medicine再生医療用の細胞足場材料への応用を目指したシルクの機能化. Sen'i Gakkaishi 78:6, pages 240-244.
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Meiyu Wu, Shenyu Huang, Xiaogang Ye, Jinghua Ruan, Shuo Zhao, Juan Ye & Boxiong Zhong. (2021) Human epidermal growth factor-functionalized cocoon silk with improved cell proliferation activity for the fabrication of wound dressings. Journal of Biomaterials Applications 36:4, pages 722-730.
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T. ErikssonC.J. Picard. (2021) Genetic and genomic selection in insects as food and feed. Journal of Insects as Food and Feed 7:5, pages 661-682.
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Mai Watanabe, Ujjal K. Bhawal, Shinji Takemoto, Norihiro Nishiyama, Yuichi Nakahara, Ken‐ichiro Tatematsu, Hideki Sezutsu, Nobuo Kuwabara, Tamiko Minamisawa, Kiyotaka Shiba & Tetsuo Asakura. (2021) Bio‐functionalized titanium surfaces with modified silk fibroin carrying titanium binding motif to enhance the ossific differentiation of MC3T3‐E1. Biotechnology and Bioengineering 118:7, pages 2585-2596.
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Yuancheng Wang, Feng Wang, Sheng Xu, Riyuan Wang, Chi Tian, Yanting Ji, Qianqian Yang, Ping Zhao & Qingyou Xia. (2020) Transdermal peptide conjugated to human connective tissue growth factor with enhanced cell proliferation and hyaluronic acid synthesis activities produced by a silkworm silk gland bioreactor. Applied Microbiology and Biotechnology 104:23, pages 9979-9990.
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Jung Woo Leem, Malcolm J. Fraser & Young L. Kim. (2020) Transgenic and Diet-Enhanced Silk Production for Reinforced Biomaterials: A Metamaterial Perspective. Annual Review of Biomedical Engineering 22:1, pages 79-102.
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Natuo Kômoto & Shuichiro Tomita. 2020. GMOs. GMOs 223 237 .
Tetsuo Asakura, Takashi Tanaka & Ryo Tanaka. (2019) Advanced Silk Fibroin Biomaterials and Application to Small-Diameter Silk Vascular Grafts. ACS Biomaterials Science & Engineering 5:11, pages 5561-5577.
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Tamara Bernadette Aigner, Elise DeSimone & Thomas Scheibel. (2018) Biomedical Applications of Recombinant Silk-Based Materials. Advanced Materials 30:19, pages 1704636.
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Mitsuru Sato, Hiroshi Kitani & Katsura Kojima. (2017) Development and validation of scFv-conjugated affinity silk protein for specific detection of carcinoembryonic antigen. Scientific Reports 7:1.
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Toshiharu Fukayama, Yusuke Ozai, Haruka Shimokawatoko, Yusuke Kimura, Derya Aytemiz, Ryou Tanaka, Noboru Machida & Tetsuo Asakura. (2017) Evaluation of endothelialization in the center part of graft using 3 cm vascular grafts implanted in the abdominal aortae of the rat. Journal of Artificial Organs 20:3, pages 221-229.
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Aurora Montali, Davide Romanelli, Silvia Cappellozza, Annalisa Grimaldi, Magda de Eguileor & Gianluca Tettamanti. (2017) Timing of autophagy and apoptosis during posterior silk gland degeneration in Bombyx mori. Arthropod Structure & Development 46:4, pages 518-528.
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Hideki SEZUTSU. (2017) Amazing Silkworm! Glowing Silk Produced by Transgenic Silkworm. Hyomen Kagaku 38:3, pages 135-137.
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Eiichi Hirakata, Naohide Tomita, Yasushi Tamada, Toru Suguro, Masaaki Nakajima, Yusuke Kambe, Keisuke Yamada, Koji Yamamoto, Masahiro Kawakami, Akihisa Otaka, Hideo Okumura & Shigehiko Suzuki. (2016) Early tissue formation on whole-area osteochondral defect of rabbit patella by covering with fibroin sponge. Journal of Biomedical Materials Research Part B: Applied Biomaterials 104:7, pages 1474-1482.
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Huan Wang, Yulong Wang, Chengjia Wu, Hui Tao, Xuedong Chen, Weimin Yin, Yanghu Sima, Yujun Wang & Shiqing Xu. (2016) Changes in 30K protein synthesis during delayed degeneration of the silk gland by a caspase-dependent pathway in a Bombyx (silkworm) mutant. Journal of Comparative Physiology B 186:6, pages 689-700.
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Shaohua Wang, Zhengying You, Mao Feng, Jiaqian Che, Yuyu Zhang, Qiujie Qian, Setsuko Komatsu & Boxiong Zhong. (2015) Analyses of the Molecular Mechanisms Associated with Silk Production in Silkworm by iTRAQ-Based Proteomics and RNA-Sequencing-Based Transcriptomics. Journal of Proteome Research 15:1, pages 15-28.
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Katsura KOJIMA. (2016) . Kagaku To Seibutsu KAGAKU TO SEIBUTSU 54:12, pages 915-919.
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Kenji Yukuhiro, Hideki Sezutsu, Takuya Tsubota, Yoko Takasu, Tsunenori Kameda & Naoyuki Yonemura. 2016. Extracellular Composite Matrices in Arthropods. Extracellular Composite Matrices in Arthropods 515 555 .
Yusuke Kambe, Katsura Kojima, Yasushi Tamada, Naohide Tomita & Tsunenori Kameda. (2016) Silk fibroin sponges with cell growth-promoting activity induced by genetically fused basic fibroblast growth factor. Journal of Biomedical Materials Research Part A 104:1, pages 82-93.
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Elena Doblhofer, Aniela Heidebrecht & Thomas Scheibel. (2015) To spin or not to spin: spider silk fibers and more. Applied Microbiology and Biotechnology 99:22, pages 9361-9380.
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Hideaki Mabashi-Asazuma, Bong-Hee Sohn, Young-Soo Kim, Chu-Wei Kuo, Kay-Hooi Khoo, Cheryl A. Kucharski, Malcolm J. FraserJr.Jr. & Donald L. Jarvis. (2015) Targeted glycoengineering extends the protein N-glycosylation pathway in the silkworm silk gland. Insect Biochemistry and Molecular Biology 65, pages 20-27.
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Huan Wang, Lu Wang, Yulong Wang, Hui Tao, Weimin Yin, Yanghu SiMa, Yujun Wang & Shiqing Xu. (2015) High yield exogenous protein HPL production in the Bombyx mori silk gland provides novel insight into recombinant expression systems. Scientific Reports 5:1.
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Valeriya Zabelina, Vyacheslav Klymenko, Toshiki Tamura, Karina Doroshenko, Haoyuan Liang, Hideki Sezutsu & František Sehnal. (2015) Genome engineering and parthenocloning in the silkworm, Bombyx mori. Journal of Biosciences 40:3, pages 645-655.
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Min Ni, FanChi Li, JiangHai Tian, JingSheng Hu, Hua Zhang, KaiZun Xu, BinBin Wang, YangYang Li, WeiDe Shen & Bing Li. (2015) Effects of Titanium Dioxide Nanoparticles on the Synthesis of Fibroin in Silkworm (Bombyx mori). Biological Trace Element Research 166:2, pages 225-235.
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Hidetoshi Teramoto & Katsura Kojima. (2015) Incorporation of Methionine Analogues Into Bombyx mori Silk Fibroin for Click Modifications . Macromolecular Bioscience 15:5, pages 719-727.
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Dingpei Long, Weijian Lu, Yuli Zhang, Lihui Bi, Zhonghuai Xiang & Aichun Zhao. (2015) An efficient strategy for producing a stable, replaceable, highly efficient transgene expression system in silkworm, Bombyx mori. Scientific Reports 5:1.
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Muhammad S. Zafar, David J. Belton, Benjamin Hanby, David L. Kaplan & Carole C. Perry. (2015) Functional Material Features of Bombyx mori Silk Light versus Heavy Chain Proteins . Biomacromolecules 16:2, pages 606-614.
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Dingpei Long, Weijian Lu, Yang Zhang, Qing Guo, Zhonghuai Xiang & Aichun Zhao. (2015) New insight into the mechanism underlying fibroin secretion in silkworm, Bombyx mori . FEBS Journal 282:1, pages 89-101.
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Toshiki Saotome, Haruki Hayashi, Ryo Tanaka, Atsushi Kinugasa, Shouji Uesugi, Ken-ichiro Tatematsu, Hideki Sezutsu, Nobuo Kuwabara & Tetsuo Asakura. (2015) Introduction of VEGF or RGD sequences improves revascularization properties of Bombyx mori silk fibroin produced by transgenic silkworm. Journal of Materials Chemistry B 3:35, pages 7109-7116.
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Zhen Li, Yue Jiang, Guangli Cao, Jingzhi Li, Renyu Xue & Chengliang Gong. (2014) Construction of transgenic silkworm spinning antibacterial silk with fluorescence. Molecular Biology Reports 42:1, pages 19-25.
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Yasushi Tamada & Katsura Kojima. 2015. Handbook of Smart Textiles. Handbook of Smart Textiles 209 224 .
Hanfu Xu. (2014) The advances and perspectives of recombinant protein production in the silk gland of silkworm Bombyx mori. Transgenic Research 23:5, pages 697-706.
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Yoshihiko Kuwana, Hideki Sezutsu, Ken-ichi Nakajima, Yasushi Tamada & Katsura Kojima. (2014) High-Toughness Silk Produced by a Transgenic Silkworm Expressing Spider (Araneus ventricosus) Dragline Silk Protein. PLoS ONE 9:8, pages e105325.
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Guoping Huang, Danfeng Yang, Chunfeng Sun, Jianping Huang, Keping Chen, Chunxia Zhang, Huiqing Chen & Qin Yao. (2014) A quicker degradation rate is yielded by a novel kind of transgenic silk fibroin consisting of shortened silk fibroin heavy chains fused with matrix metalloproteinase cleavage sites. Journal of Materials Science: Materials in Medicine 25:8, pages 1833-1842.
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Michael Wöltje, Melanie Böbel, Michael Rheinnecker, Gianluca Tettamanti, Eleonora Franzetti, Alessio Saviane & Silvia Cappellozza. (2014) Transgenic protein production in silkworm silk glands requires cathepsin and chitinase of Autographa californica multicapsid nucleopolyhedrovirus. Applied Microbiology and Biotechnology 98:10, pages 4571-4580.
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Olena Tokareva, Matthew Jacobsen, Markus Buehler, Joyce Wong & David L. Kaplan. (2014) Structure–function–property–design interplay in biopolymers: Spider silk. Acta Biomaterialia 10:4, pages 1612-1626.
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Mitsuru Sato, Katsura Kojima, Chisato Sakuma, Maria Murakami, Yasushi Tamada & Hiroshi Kitani. (2014) Production of scFv-conjugated affinity silk film and its application to a novel enzyme-linked immunosorbent assay. Scientific Reports 4:1.
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Tetsuo Asakura, Makoto Isozaki, Toshiki Saotome, Ken-ichiro Tatematsu, Hideki Sezutsu, Nobuo Kuwabara & Yasumoto Nakazawa. (2014) Recombinant silk fibroin incorporated cell-adhesive sequences produced by transgenic silkworm as a possible candidate for use in vascular graft. J. Mater. Chem. B 2:42, pages 7375-7383.
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Derya Aytemiz & Tetsuo Asakura. 2014. Biotechnology of Silk. Biotechnology of Silk 69 85 .
Jong-Hwa Park, Sung-Wan Kim, Young-Hun Jeong, Jong-Kil Lee, Young-Mi Go, Sang-Chan Lee, Kwang-Ho Choi, Seong-Ryul Kim & Tae-Won Goo. (2013) Reeling of recombinant flourescence cocoons through low temperature decompressed cooking. Korean Journal of Sericultural Science 51:2, pages 142-146.
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Tetsuya Iizuka, Hideki Sezutsu, Ken-ichiro Tatematsu, Isao Kobayashi, Naoyuki Yonemura, Keiro Uchino, Kenichi Nakajima, Katsura Kojima, Chiyuki Takabayashi, Hiroaki Machii, Katsushige Yamada, Hiroyuki Kurihara, Tetsuo Asakura, Yasumoto Nakazawa, Atsushi Miyawaki, Satoshi Karasawa, Hatsumi Kobayashi, Junji Yamaguchi, Nobuo Kuwabara, Takashi Nakamura, Kei Yoshii & Toshiki Tamura. (2013) Colored Fluorescent Silk Made by Transgenic Silkworms. Advanced Functional Materials 23:42, pages 5232-5239.
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Olena Tokareva, Valquíria A. Michalczechen‐Lacerda, Elíbio L. Rech & David L. Kaplan. (2013) Recombinant DNA production of spider silk proteins . Microbial Biotechnology 6:6, pages 651-663.
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Mitsuru Sato, Katsura Kojima, Chisato Sakuma, Maria Murakami, Eriko Aratani, Takato Takenouchi, Yasushi Tamada & Hiroshi Kitani. (2012) Production of scFv-Conjugated Affinity Silk Powder by Transgenic Silkworm Technology. PLoS ONE 7:4, pages e34632.
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Florence TeuléYun-Gen Miao, Bong-Hee Sohn, Young-Soo Kim, J. Joe HullMalcolm J. FraserJr.Jr., Randolph V. LewisDonald L. Jarvis. (2012) Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proceedings of the National Academy of Sciences 109:3, pages 923-928.
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Masahiro Tomita. (2010) Transgenic silkworms that weave recombinant proteins into silk cocoons. Biotechnology Letters 33:4, pages 645-654.
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Aya Nagano, Yumi Tanioka, Nobuko Sakurai, Hideki Sezutsu, Noboru Kuboyama, Hideo Kiba, Yasuhiro Tanimoto, Norihiro Nishiyama & Tetsuo Asakura. (2011) Regeneration of the femoral epicondyle on calcium-binding silk scaffolds developed using transgenic silk fibroin produced by transgenic silkworm. Acta Biomaterialia 7:3, pages 1192-1201.
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František Sehnal. 2011. Insect Biotechnology. Insect Biotechnology 211 224 .
Yusuke Kambe, Koji Yamamoto, Katsura Kojima, Yasushi Tamada & Naohide Tomita. (2010) Effects of RGDS sequence genetically interfused in the silk fibroin light chain protein on chondrocyte adhesion and cartilage synthesis. Biomaterials 31:29, pages 7503-7511.
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Ken-ichiro Tatematsu, Isao Kobayashi, Keiro Uchino, Hideki Sezutsu, Tetsuya Iizuka, Naoyuki Yonemura & Toshiki Tamura. (2009) Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Research 19:3, pages 473-487.
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Isao Kobayashi, Katsura Kojima, Hideki Sezutsu, Keiro Uchino & Toshiki Tamura. (2009) Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori . Insect Science 16:6, pages 465-473.
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