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Bioscience, Biotechnology, and Biochemistry Volume 74, 2010 - Issue 3
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Original Articles

Simple and Effective Gap-Repair Cloning Using Short Tracts of Flanking Homology in Fission Yeast

Yuzy MATSUODepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
,
Hayafumi KISHIMOTODepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
,
Tomitaka HORIUCHIDepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
,
Katsuhiro TANAEDepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
&
Makoto KAWAMUKAIDepartment of Applied Bioscience and Biotechnology, Faculty of Life and Environmental Science, Shimane University
Pages 685-689 | Received 28 Dec 2009, Accepted 02 Feb 2010, Published online: 22 May 2014

Citations (16)

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Kimihiko Mizutani. (2015) High-throughput plasmid construction using homologous recombination in yeast: its mechanisms and application to protein production for X-ray crystallography. Bioscience, Biotechnology, and Biochemistry 79:1, pages 1-10.
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Articles from other publishers (15)

Ryosuke Mitsui & Ryosuke Yamada. 2021. Microbial Cell Factories Engineering for Production of Biomolecules. Microbial Cell Factories Engineering for Production of Biomolecules 319 333 .
Xiao-Ran Zhang, Jia-Bei He, Yi-Zheng Wang & Li-Lin Du. (2018) A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast. G3 Genes|Genomes|Genetics 8:6, pages 2067-2077.
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Yunqi Ma, Ming Lu, Ziwei Chang, Chu Lee, Hae-Kyun Yoo & Jang-Su Park. (2018) Expression Procedure Optimization of Carassius aurantus CYP1A in Shewanella Using Plasmid Construction Strategy. Biotechnology and Bioprocess Engineering 23:1, pages 86-92.
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Fanli Zeng, Zhimin Hao, Pan Li, Yanan Meng, Jingao Dong & Yibin Lin. (2017) A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends. BMC Biotechnology 17:1.
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Daisuke Moriyama, Tomohiro Kaino, Kazuyoshi Yajima, Ryota Yanai, Yasuhiro Ikenaka, Junzo Hasegawa, Motohisa Washida, Hirokazu Nanba & Makoto Kawamukai. (2016) Cloning and characterization of decaprenyl diphosphate synthase from three different fungi. Applied Microbiology and Biotechnology 101:4, pages 1559-1571.
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Ken Motohashi. 2017. In Vitro Mutagenesis. In Vitro Mutagenesis 349 357 .
Ken Motohashi. (2015) A simple and efficient seamless DNA cloning method using SLiCE from Escherichia coli laboratory strains and its application to SLiP site-directed mutagenesis. BMC Biotechnology 15:1.
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Mary W. Corrigan, Christine L. Kerwin-Iosue, Alexander S. Kuczmarski, Kunj B. Amin & Dennis D. Wykoff. (2013) The Fate of Linear DNA in Saccharomyces cerevisiae and Candida glabrata: The Role of Homologous and Non-Homologous End Joining. PLoS ONE 8:7, pages e69628.
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Tomohiko Matsuzawa, Youko Kageyama, Kazuyoshi Ooishi, Makoto Kawamukai & Kaoru Takegawa. (2013) The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast. FEMS Yeast Research 13:3, pages 259-266.
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Yuzy Matsuo, Kouhei Nishino, Kouhei Mizuno, Takashi Akihiro, Takashi Toda, Yasuhiro Matsuo, Tomohiro Kaino & Makoto Kawamukai. (2013) Polypeptone Induces Dramatic Cell Lysis in ura4 Deletion Mutants of Fission Yeast. PLoS ONE 8:3, pages e59887.
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Katsuhiro Tanae, Tomitaka Horiuchi, Takuya Yamakawa, Yuzy Matsuo & Makoto Kawamukai. (2012) Sim3 shares some common roles with the histone chaperone Asf1 in fission yeast. FEBS Letters 586:23, pages 4190-4196.
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Katsuhiro Tanae, Tomitaka Horiuchi, Yuzy Matsuo, Satoshi Katayama & Makoto Kawamukai. (2012) Histone Chaperone Asf1 Plays an Essential Role in Maintaining Genomic Stability in Fission Yeast. PLoS ONE 7:1, pages e30472.
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Ana A. Kitazono. (2011) Optimized protocols and plasmids for in vivo cloning in yeast. Gene 484:1-2, pages 86-89.
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Yuzy Matsuo, Hayafumi Kishimoto, Katsuhiro Tanae, Kenji Kitamura, Satoshi Katayama & Makoto Kawamukai. (2011) Nuclear Protein Quality Is Regulated by the Ubiquitin-Proteasome System through the Activity of Ubc4 and San1 in Fission Yeast. Journal of Biological Chemistry 286:15, pages 13775-13790.
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. (2010) Current awareness on yeast. Yeast 27:8, pages 685-692.
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