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Latest approaches for efficient protein production in drug discovery

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Changjun Liu, Qing Yan, Ke Yi, Tianhao Hu, Jianjie Wang, Zheyang Zhang, Huimin Li, Yutao Luo, Dongyi Zhang & Er Meng. (2023) A secretory system for extracellular production of spider neurotoxin huwentoxin-I in Escherichia coli. Preparative Biochemistry & Biotechnology 53:8, pages 914-922.
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Nicola Scafuri, Miguel A. Soler, Andrea Spitaleri & Walter Rocchia. (2021) Enhanced Molecular Dynamics Method to Efficiently Increase the Discrimination Capability of Computational Protein–Protein Docking. Journal of Chemical Theory and Computation 17:11, pages 7271-7280.
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Riccardo Concu, M. Natália. D. S. Cordeiro, Cristian R. Munteanu & Humbert González-Díaz. (2019) PTML Model of Enzyme Subclasses for Mining the Proteome of Biofuel Producing Microorganisms. Journal of Proteome Research 18:7, pages 2735-2746.
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Thoring, Zemella, Wüstenhagen & Kubick. (2019) Accelerating the Production of Druggable Targets: Eukaryotic Cell-Free Systems Come into Focus. Methods and Protocols 2:2, pages 30.
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Sibusiso B. Maseko, Deidre Govender, Thavendran Govender, Tricia Naicker, Johnson Lin, Glenn E. M. Maguire & Hendrik G. Kruger. (2019) Optimized Procedure for Recovering HIV-1 Protease (C-SA) from Inclusion Bodies. The Protein Journal 38:1, pages 30-36.
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David J. Wurm, Lukas Marschall, Patrick Sagmeister, Christoph Herwig & Oliver Spadiut. (2017) Simple monitoring of cell leakiness and viability in Escherichia coli bioprocesses-A case study . Engineering in Life Sciences 17:6, pages 598-604.
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Christopher H. Gray, Jennifer Konczal, Mokdad Mezna, Shehab Ismail, Justin Bower & Martin Drysdale. (2017) A fully automated procedure for the parallel, multidimensional purification and nucleotide loading of the human GTPases KRas, Rac1 and RalB. Protein Expression and Purification 132, pages 75-84.
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Toshihiko Sugiki, Naohiro Kobayashi & Toshimichi Fujiwara. (2017) Modern Technologies of Solution Nuclear Magnetic Resonance Spectroscopy for Three-dimensional Structure Determination of Proteins Open Avenues for Life Scientists. Computational and Structural Biotechnology Journal 15, pages 328-339.
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Vignesh Rajamanickam, David Wurm, Christoph Slouka, Christoph Herwig & Oliver Spadiut. (2016) A novel toolbox for E. coli lysis monitoring. Analytical and Bioanalytical Chemistry 409:3, pages 667-671.
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Anna Vangone, Romina Oliva, Luigi Cavallo & Alexandre M. J. J. Bonvin. 2017. From Protein Structure to Function with Bioinformatics. From Protein Structure to Function with Bioinformatics 265 292 .
Toshihiko Sugiki, Toshimichi Fujiwara & Chojiro Kojima. 2017. Heterologous Gene Expression in E.coli. Heterologous Gene Expression in E.coli 345 357 .
Karina T. O. Santana-Jorge, Túlio M. Santos, Natayme R. Tartaglia, Edgar L. Aguiar, Renata F. S. Souza, Ricardo B. Mariutti, Raphael J. Eberle, Raghuvir K. Arni, Ricardo W. Portela, Roberto Meyer & Vasco Azevedo. (2016) Putative virulence factors of Corynebacterium pseudotuberculosis FRC41: vaccine potential and protein expression. Microbial Cell Factories 15:1.
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S.A. Gray, R.N. Coler, D. Carter & A.A. Siddiqui. 2016. Host-Microbe Interactions. Host-Microbe Interactions 291 315 .
Anton A. Komar. 2017. Protein Therapeutics. Protein Therapeutics 161 177 .
Anna Vangone & Alexandre MJJ Bonvin. (2015) Contacts-based prediction of binding affinity in protein–protein complexes. eLife 4.
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