513
Views
54
CrossRef citations to date
0
Altmetric
Review

In vitro lung slices: a powerful approach for assessment of lung pathophysiology

, &
Pages 501-508 | Published online: 09 Jan 2014

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (3)

Angela Temann, Tatiana Golovina, Vanessa Neuhaus, Carolann Thompson, Jessica A. Chichester, Armin Braun & Vidadi Yusibov. (2017) Evaluation of inflammatory and immune responses in long-term cultured human precision-cut lung slices. Human Vaccines & Immunotherapeutics 13:2, pages 351-358.
Read now
Hassiba Smail, Jean-Marc Baste, Arnaud Gay, Hugues Begueret, Romain Noël, Jean-Paul Morin & Pierre-Yves Litzler. (2016) Role of inflammatory cells and adenosine in lung ischemia reoxygenation injury using a model of lung donation after cardiac death. Experimental Lung Research 42:3, pages 131-141.
Read now
Jean-Paul Morin, Jean-Marc Baste, Arnaud Gay, Clément Crochemore, Cécile Corbière & Christelle Monteil. (2013) Precision cut lung slices as an efficient tool for in vitro lung physio-pharmacotoxicology studies. Xenobiotica 43:1, pages 63-72.
Read now

Articles from other publishers (51)

Åsa Gustafson, Linda Elfsmark, Terese Karlsson & Sofia Jonasson. (2023) N-acetyl cysteine mitigates lung damage and inflammation after chlorine exposure in vivo and ex vivo. Toxicology and Applied Pharmacology 479, pages 116714.
Crossref
Érika Yoko Suzuki, Alice Simon, Thaisa Francielle Souza Domingos, Bárbara de Azevedo Abrahim Vieira, Alessandra Mendonça Teles de Souza, Carlos Rangel Rodrigues, Valeria Pereira de Sousa, Flávia Almada do Carmo & Lucio Mendes Cabral. (2023) Alternative Methods for Pulmonary-Administered Drugs Metabolism: A Breath of Change. Mini-Reviews in Medicinal Chemistry 23:2, pages 170-186.
Crossref
Luisa Ragionieri, Enrica Scalera, Matteo Zoboli, Roberta Ciccimarra, Giulia Petracco, Ferdinando Gazza, Antonio Cacchioli, Matteo Storti, Chiara Catozzi, Francesca Ricci & Francesca Ravanetti. (2023) Preterm rabbit-derived Precision Cut Lung Slices as alternative model of bronchopulmonary dysplasia in preclinical study: a morphological fine-tuning approach. Annals of Anatomy - Anatomischer Anzeiger 246, pages 152039.
Crossref
Sandra Runft, Iris Färber, Johannes Krüger, Nadine Krüger, Federico Armando, Cheila Rocha, Stefan Pöhlmann, Laura Burigk, Eva Leitzen, Malgorzata Ciurkiewicz, Armin Braun, Daniel Schneider, Lars Baumgärtner, Bernd Freisleben & Wolfgang Baumgärtner. (2022) Alternatives to animal models and their application in the discovery of species susceptibility to SARS-CoV-2 and other respiratory infectious pathogens: A review. Veterinary Pathology 59:4, pages 565-577.
Crossref
Ranu Surolia, Kevin G. Dsouza & Veena B. Antony. 2022. Tissue Engineering. Tissue Engineering 627 646 .
Jonas Tigges, Franz Worek, Horst Thiermann & Timo Wille. (2021) Organophosphorus pesticides exhibit compound specific effects in rat precision-cut lung slices (PCLS): mechanisms involved in airway response, cytotoxicity, inflammatory activation and antioxidative defense. Archives of Toxicology 96:1, pages 321-334.
Crossref
Jonas Tigges, Florian Eggerbauer, Franz Worek, Horst Thiermann, Ursula Rauen & Timo Wille. (2021) Optimization of long-term cold storage of rat precision-cut lung slices with a tissue preservation solution. American Journal of Physiology-Lung Cellular and Molecular Physiology 321:6, pages L1023-L1035.
Crossref
Lina Ågren, Linda Elfsmark, Christine Akfur & Sofia Jonasson. (2021) High concentrations of ammonia induced cytotoxicity and bronchoconstriction in a precision-cut lung slices rat model. Toxicology Letters 349, pages 51-60.
Crossref
Mohammed Ali Selo, Johannes A. Sake, Kwang-Jin Kim & Carsten Ehrhardt. (2021) In vitro and ex vivo models in inhalation biopharmaceutical research — advances, challenges and future perspectives. Advanced Drug Delivery Reviews 177, pages 113862.
Crossref
Inge Brouns, Line Verckist, Isabel Pintelon, Jean-Pierre Timmermans & Dirk AdriaensenInge Brouns, Line Verckist, Isabel Pintelon, Jean-Pierre Timmermans & Dirk Adriaensen. 2021. The Pulmonary Neuroepithelial Body Microenvironment. The Pulmonary Neuroepithelial Body Microenvironment 19 29 .
Inge Brouns, Line Verckist, Isabel Pintelon, Jean-Pierre Timmermans & Dirk AdriaensenInge Brouns, Line Verckist, Isabel Pintelon, Jean-Pierre Timmermans & Dirk Adriaensen. 2021. The Pulmonary Neuroepithelial Body Microenvironment. The Pulmonary Neuroepithelial Body Microenvironment 1 65 .
Gerald Burgstaller, Michael Gerckens, Oliver Eickelberg & Melanie Königshoff. 2021. Myofibroblasts. Myofibroblasts 447 456 .
Tamara Cruz, Ana L. Mora & Mauricio Rojas. 2021. Myofibroblasts. Myofibroblasts 139 145 .
Daniel L. Matera, Katarina M. DiLillo, Makenzee R. Smith, Christopher D. Davidson, Ritika Parikh, Mohammed Said, Carole A. WilkeIsabelle M. Lombaert, Kelly B. Arnold, Bethany B. MooreBrendon M. Baker. (2020) Microengineered 3D pulmonary interstitial mimetics highlight a critical role for matrix degradation in myofibroblast differentiation. Science Advances 6:37.
Crossref
Bwalya A. Witika, Pedzisai A. Makoni, Scott K. Matafwali, Billy Chabalenge, Chiluba Mwila, Aubrey C. Kalungia, Christian I. Nkanga, Alain M. Bapolisi & Roderick B. Walker. (2020) Biocompatibility of Biomaterials for Nanoencapsulation: Current Approaches. Nanomaterials 10:9, pages 1649.
Crossref
Hani N. Alsafadi, Franziska E. Uhl, Ricardo H. Pineda, Kolene E. Bailey, Mauricio Rojas, Darcy E. Wagner & Melanie Königshoff. (2020) Applications and Approaches for Three-Dimensional Precision-Cut Lung Slices. Disease Modeling and Drug Discovery. American Journal of Respiratory Cell and Molecular Biology 62:6, pages 681-691.
Crossref
Lina Ågren, Linda Elfsmark, Christine Akfur, Lars Hägglund, Barbro Ekstrand-Hammarström & Sofia Jonasson. (2020) N-acetyl cysteine protects against chlorine-induced tissue damage in an ex vivo model. Toxicology Letters 322, pages 58-65.
Crossref
Yildiz Yilmaz, Gareth Williams, Markus Walles, Nenad Manevski, Stephan Krähenbühl & Gian Camenisch. (2019) Comparison of Rat and Human Pulmonary Metabolism Using Precision-cut Lung Slices (PCLS). Drug Metabolism Letters 13:1, pages 53-63.
Crossref
Matea Cedilak, Mihailo Banjanac, Daniela Belamarić, Andrea Paravić Radičević, Ivan Faraho, Krunoslav Ilić, Snježana Čužić, Ines Glojnarić, Vesna Eraković Haber & Martina Bosnar. (2019) Precision-cut lung slices from bleomycin treated animals as a model for testing potential therapies for idiopathic pulmonary fibrosis. Pulmonary Pharmacology & Therapeutics 55, pages 75-83.
Crossref
María C. Rosales Gerpe, Jacob P. van Vloten, Lisa A. Santry, Jondavid de Jong, Robert C. Mould, Adrian Pelin, John C. Bell, Byram W. Bridle & Sarah K. Wootton. (2018) Use of Precision-Cut Lung Slices as an Ex Vivo Tool for Evaluating Viruses and Viral Vectors for Gene and Oncolytic Therapy. Molecular Therapy - Methods & Clinical Development 10, pages 245-256.
Crossref
Gerald Burgstaller, Arunima Sengupta, Sarah Vierkotten, Gerhard Preissler, Michael Lindner, Jürgen Behr, Melanie Königshoff & Oliver Eickelberg. (2018) Distinct niches within the extracellular matrix dictate fibroblast function in (cell free) 3D lung tissue cultures. American Journal of Physiology-Lung Cellular and Molecular Physiology 314:5, pages L708-L723.
Crossref
Julia Herbert, Horst Thiermann, Franz Worek & Timo Wille. (2017) Precision cut lung slices as test system for candidate therapeutics in organophosphate poisoning. Toxicology 389, pages 94-100.
Crossref
Esther Y Cheah, Tracy S Mann, Philip C Burcham & Peter J Henry. (2017) Functional characterisation and application of an ex vivo perfusion-superfusion system in murine airways. Journal of Pharmacological and Toxicological Methods 84, pages 66-77.
Crossref
Caitlin L. Maikawa, Naomi Zimmerman, Khaled Rais, Mittal Shah, Brie Hawley, Pallavi Pant, Cheol-Heon Jeong, Juana Maria Delgado-Saborit, John Volckens, Greg Evans, James S. Wallace & Krystal J. Godri Pollitt. (2016) Murine precision-cut lung slices exhibit acute responses following exposure to gasoline direct injection engine emissions. Science of The Total Environment 568, pages 1102-1109.
Crossref
Chantal Donovan, Huei Jiunn Seow, Jane E. Bourke & Ross Vlahos. (2016) Influenza A virus infection and cigarette smoke impair bronchodilator responsiveness to β-adrenoceptor agonists in mouse lung. Clinical Science 130:10, pages 829-837.
Crossref
Erica Bäckström, Anders Lundqvist, Elin Boger, Petter Svanberg, Pär Ewing, Margareta Hammarlund-Udenaes & Markus Fridén. (2016) Development of a Novel Lung Slice Methodology for Profiling of Inhaled Compounds. Journal of Pharmaceutical Sciences 105:2, pages 838-845.
Crossref
Chris Cousens, Charline Alleaume, Esther Bijsmans, Henny M Martineau, Jeanie Finlayson, Mark P Dagleish & David J Griffiths. (2015) Jaagsiekte sheep retrovirus infection of lung slice cultures. Retrovirology 12:1.
Crossref
Franziska E. Uhl, Sarah Vierkotten, Darcy E. Wagner, Gerald Burgstaller, Rita Costa, Ina Koch, Michael Lindner, Silke Meiners, Oliver Eickelberg & Melanie Königshoff. (2015) Preclinical validation and imaging of Wnt-induced repair in human 3D lung tissue cultures. European Respiratory Journal 46:4, pages 1150-1166.
Crossref
Chantal Donovan, Huei Jiunn Seow, Simon G. Royce, Jane E. Bourke & Ross Vlahos. (2015) Alteration of Airway Reactivity and Reduction of Ryanodine Receptor Expression by Cigarette Smoke in Mice. American Journal of Respiratory Cell and Molecular Biology 53:4, pages 471-478.
Crossref
Gerald Burgstaller, Sarah Vierkotten, Michael Lindner, Melanie Königshoff & Oliver Eickelberg. (2015) Multidimensional immunolabeling and 4D time-lapse imaging of vital ex vivo lung tissue. American Journal of Physiology-Lung Cellular and Molecular Physiology 309:4, pages L323-L332.
Crossref
M. Ian Gilmour, Yong Ho Kim & Michael D. Hays. (2015) Comparative chemistry and toxicity of diesel and biomass combustion emissions. Analytical and Bioanalytical Chemistry 407:20, pages 5869-5875.
Crossref
G. Youssef, W. A. H. Wallace, M. P. Dagleish, C. Cousens & D. J. Griffiths. (2015) Ovine Pulmonary Adenocarcinoma: A Large Animal Model for Human Lung Cancer. ILAR Journal 56:1, pages 99-115.
Crossref
Chantal Donovan, Simon G. Royce, Ross Vlahos & Jane E. Bourke. (2015) Lipopolysaccharide Does Not Alter Small Airway Reactivity in Mouse Lung Slices. PLOS ONE 10:3, pages e0122069.
Crossref
Peter Wick, Savvina Chortarea, Olivier T. Guenat, Matthias Roesslein, Janick D. Stucki, Stephanie Hirn, Alke Petri-Fink & Barbara Rothen-Rutishauser. (2015) In vitro-ex vivo model systems for nanosafety assessment. European Journal of Nanomedicine 7:3.
Crossref
Darcy E. Wagner, Franziska E. Uhl & Daniel J. Weiss. 2015. Stem Cells in the Lung. Stem Cells in the Lung 309 347 .
Darcy E. Wagner, Franziska E. Uhl, Melanie Königshoff & Daniel J. Weiss. 2015. Lung Stem Cells in the Epithelium and Vasculature. Lung Stem Cells in the Epithelium and Vasculature 145 180 .
Stephanie Hirn, Nadine Haberl, Kateryna Loza, Matthias Epple, Wolfgang G Kreyling, Barbara Rothen-Rutishauser, Markus Rehberg & Fritz Krombach. (2014) Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices. Beilstein Journal of Nanotechnology 5, pages 2440-2449.
Crossref
Yong Ho Kim, Haiyan Tong, Mary Daniels, Elizabeth Boykin, Q Todd Krantz, John McGee, Michael Hays, Kasey Kovalcik, Janice A Dye & M Ian Gilmour. (2014) Cardiopulmonary toxicity of peat wildfire particulate matter and the predictive utility of precision cut lung slices. Particle and Fibre Toxicology 11:1.
Crossref
Tong GuoKa Yee Lok, Changhe Yu & Zhuo Li. (2014) Lung Fibrosis: Drug Screening and Disease Biomarker Identification with a Lung Slice Culture Model and Subtracted cDNA Library. Alternatives to Laboratory Animals 42:4, pages 235-243.
Crossref
M. FitzPatrick, C. Donovan & J.E. Bourke. (2014) Prostaglandin E2 elicits greater bronchodilation than salbutamol in mouse intrapulmonary airways in lung slices. Pulmonary Pharmacology & Therapeutics 28:1, pages 68-76.
Crossref
Jane E. Bourke, Yan Bai, Chantal Donovan, James G. Esposito, Xiahui Tan & Michael J. Sanderson. (2014) Novel Small Airway Bronchodilator Responses to Rosiglitazone in Mouse Lung Slices. American Journal of Respiratory Cell and Molecular Biology 50:4, pages 748-756.
Crossref
Chantal Donovan, Simon G. Royce, James Esposito, Jenny Tran, Zaridatul Aini Ibrahim, Mimi L. K. Tang, Simon Bailey & Jane E. Bourke. (2013) Differential Effects of Allergen Challenge on Large and Small Airway Reactivity in Mice. PLoS ONE 8:9, pages e74101.
Crossref
Rose E. Dixon & Luis F. Santana. (2013) A Ca2+- and PKC-driven regulatory network in airway smooth muscle. Journal of General Physiology 141:2, pages 161-164.
Crossref
Wenxin Wu, J Leland Booth & Jordan P Metcalf. 2012. Replacing Animal Models. Replacing Animal Models 137 149 .
Ki Jung Kim & Jessica A. Filosa. (2012) Advanced in vitro approach to study neurovascular coupling mechanisms in the brain microcirculation . The Journal of Physiology 590:7, pages 1757-1770.
Crossref
Jian ZhouMartha B. Alvarez-ElizondoElliot Botvinick & Steven C. George. (2012) Local small airway epithelial injury induces global smooth muscle contraction and airway constriction. Journal of Applied Physiology 112:4, pages 627-637.
Crossref
Rita A. Trammell, Teresa A. Liberati & Linda A. Toth. (2012) Host genetic background and the innate inflammatory response of lung to influenza virus. Microbes and Infection 14:1, pages 50-58.
Crossref
Kathy Schnorbusch, Robrecht Lembrechts, Inge Brouns, Isabel Pintelon, Jean-Pierre Timmermans & Dirk Adriaensen. 2012. Arterial Chemoreception. Arterial Chemoreception 157 166 .
Inge Brouns, Isabel Pintelon, Jean-Pierre Timmermans & Dirk AdriaensenInge Brouns, Isabel Pintelon, Jean-Pierre Timmermans & Dirk Adriaensen. 2012. Novel Insights in the Neurochemistry and Function of Pulmonary Sensory Receptors. Novel Insights in the Neurochemistry and Function of Pulmonary Sensory Receptors 79 89 .
Michael J. Sanderson. (2011) Exploring lung physiology in health and disease with lung slices. Pulmonary Pharmacology & Therapeutics 24:5, pages 452-465.
Crossref
Melanie K?nigshoff, Franziska Uhl & Reinoud Gosens. (2011) From molecule to man: Integrating molecular biology with whole organ physiology in studying respiratory disease. Pulmonary Pharmacology & Therapeutics 24:5, pages 466-470.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.