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Special Report

Limitations and advantages of MS-HRM and bisulfite sequencing for single locus methylation studies

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Pages 575-580 | Published online: 09 Jan 2014

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Surbhi Jain, Tomasz K Wojdacz & Ying-Hsiu Su. (2013) Challenges for the application of DNA methylation biomarkers in molecular diagnostic testing for cancer. Expert Review of Molecular Diagnostics 13:3, pages 283-294.
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Olga Taryma-Lesniak, Tina E. Kjeldsen, Lise Lotte Hansen & Tomasz K. Wojdacz. (2022) Influence of Unequal Amplification of Methylated and Non-Methylated Template on Performance of Pyrosequencing. Genes 13:8, pages 1418.
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Ali Javadmanesh, Afsaneh Mojtabanezhad Shariatpanahi, Ehsan Shams Davodly, Marjan Azghandi, Maryam Yassi, Mehdi Heidari, Matin Kerachian & Mohammad Amin Kerachian. (2022) MS-HRM protocol: a simple and low-cost approach for technical validation of next-generation methylation sequencing data. Molecular Genetics and Genomics 297:4, pages 1101-1109.
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Liu Dong, Yingfeng Zhu, Liting Wu, Qiaoan Zhang, Feng Xu, Xinju Zhang, Xiao Xu, Yiting Tang, Guoqiang Ren, Zhihua Kang & Ming Guan. (2021) Detection of MSH2 Gene Methylation in Extramammary Paget’s Disease by Methylation-Sensitive High-Resolution Melting Analysis. Journal of Oncology 2021, pages 1-7.
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Robert C. Peery, Mohan Pammi, Erika Claud & Lanlan Shen. (2021) Epigenome – A mediator for host-microbiome crosstalk. Seminars in Perinatology 45:6, pages 151455.
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Xiumei Xing, Zhini He, Ziwei Wang, Ziying Mo, Liping Chen, Boyi Yang, Zhengbao Zhang, Shen Chen, Lizhu Ye, Rui Zhang, Yuxin Zheng, Wen Chen & Daochuan Li. (2020) Association between H3K36me3 modification and methylation of LINE-1 and MGMT in peripheral blood lymphocytes of PAH-exposed workers. Toxicology Research 9:5, pages 661-668.
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Karolina Prajzendanc, Paweł Domagała, Jolanta Hybiak, Janusz Ryś, Tomasz Huzarski, Marek Szwiec, Joanna Tomiczek‐Szwiec, Wojciech Redelbach, Aleksandra Sejda, Jacek Gronwald, Tomasz Kluz, Rafał Wiśniowski, Cezary Cybulski, Alicja Łukomska, Katarzyna Białkowska, Grzegorz Sukiennicki, Katarzyna Kulczycka, Steven A. Narod, Tomasz K. Wojdacz, Jan Lubiński & Anna Jakubowska. (2019) BRCA1 promoter methylation in peripheral blood is associated with the risk of triple‐negative breast cancer . International Journal of Cancer 146:5, pages 1293-1298.
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Farzeen Kader, Meenu Ghai & Ademola O. Olaniran. (2019) Characterization of DNA methylation-based markers for human body fluid identification in forensics: a critical review. International Journal of Legal Medicine 134:1, pages 1-20.
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Mostafa Kamal Masud, Jongbeom Na, Muhammad Younus, Md. Shahriar A. Hossain, Yoshio Bando, Muhammad J. A. Shiddiky & Yusuke Yamauchi. (2019) Superparamagnetic nanoarchitectures for disease-specific biomarker detection. Chemical Society Reviews 48:24, pages 5717-5751.
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Wiphawan Wasenang, Ponlatham Chaiyarit, Siriporn Proungvitaya & Temduang Limpaiboon. (2019) Serum cell-free DNA methylation of OPCML and HOXD9 as a biomarker that may aid in differential diagnosis between cholangiocarcinoma and other biliary diseases. Clinical Epigenetics 11:1.
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Yu-Xia Zhan & Guang-Hua Luo. (2019) DNA methylation detection methods used in colorectal cancer. World Journal of Clinical Cases 7:19, pages 2916-2929.
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Jingchao Ren, Jun-peng Cui, Mengkai Luo, Huan Liu, Pengfei Hao, Xiao Wang & Guang-hui Zhang. (2019) The prevalence and persistence of aberrant promoter DNA methylation in benzene-exposed Chinese workers. PLOS ONE 14:8, pages e0220500.
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Chetan Bakshi, Rajesh Vijayvergiya & Veena Dhawan. (2019) Aberrant DNA methylation of M1-macrophage genes in coronary artery disease. Scientific Reports 9:1.
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Yu Zhang, Weiliang Shen, Mingyue Cao, Juan Li, Baoxiao Zheng, Zhengjia Lou, Baoying Qian & Liangyi Xue. (2019) Dynamic alterations in methylation of global DNA and growth-related genes in large yellow croaker (Larimichthys crocea) in response to starvation stress. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 227, pages 98-105.
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Iben Daugaard, Dianna Hussmann, Louise Kristensen, Thomas Kristensen, Tina E Kjeldsen, Charlotte G Nyvold, Thomas S Larsen, Michael B Møller, Lise Lotte Hansen & Tomasz K Wojdacz. (2018) Chronic lymphocytic leukemia patients with heterogeneously or fully methylated LPL promotor display longer time to treatment . Epigenomics 10:9, pages 1155-1166.
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Diana Chávez-Lizárraga, Paola Zárate-Segura, Jorge Beltrán-Montoya, Carmen Canchola-Sotelo, Felipe Vadillo-Ortega & Erika Chavira-Suárez. (2018) DNA Methylation Variability in a Single Locus of the RXRα Promoter from Umbilical Vein Blood at Term Pregnancy. Biochemical Genetics 56:3, pages 210-224.
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Lu-Lu Sun, Yuan Liu, Xiao Sun, Lei Pan, Dan Wu & Yu-Dong Wang. (2018) Limited Role of Promoter Methylation of MGMT and C13ORF18 in Triage of Low-Grade Squamous Intraepithelial Lesion. Chinese Medical Journal 131:8, pages 939-944.
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Han-Lu Gao, Xuan Wang, Hong-Ru Sun, Jun-De Zhou, Shang-Qun Lin, Yu-Hang Xing, Lin Zhu, Hai-Bo Zhou, Ya-Shuang Zhao, Qiang Chi & Yu-Peng Liu. (2018) Methylation Status of Transcriptional Modulatory Genes Associated with Colorectal Cancer in Northeast China. Gut and Liver 12:2, pages 173-182.
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Dianna Hussmann & Lise Lotte Hansen. 2018. DNA Methylation Protocols. DNA Methylation Protocols 551 571 .
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Iben Daugaard, Alice Knudsen, Tina E. Kjeldsen, Henrik Hager & Lise Lotte Hansen. (2017) The association between miR-34 dysregulation and distant metastases formation in lung adenocarcinoma. Experimental and Molecular Pathology 102:3, pages 484-491.
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Byeong-Min Park, Ok-Jin Yoon & Do-Hoon Lee. (2017) Global DNA Methylation Patterns and Gene Expression Associated with Obesity-Susceptibility in Offspring of Pregnant Sprague-Dawley Rats Exposed to BDE-47 and BDE-209. The Korean Journal of Clinical Laboratory Science 49:1, pages 28-39.
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David Cheishvili, Sophie Petropoulos, Steffan Christiansen & Moshe Szyf. 2017. Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases. Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases 33 50 .
Nina Teroganova, Leah Girshkin, Catherine M. Suter & Melissa J. Green. (2016) DNA methylation in peripheral tissue of schizophrenia and bipolar disorder: a systematic review. BMC Genetics 17:1.
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Huiqi Li, Gabriella Åkerman, Carola Lidén, Ayman Alhamdow, Tomasz K. Wojdacz, Karin Broberg & Maria Albin. (2016) Alterations of telomere length and DNA methylation in hairdressers: A cross-sectional study. Environmental and Molecular Mutagenesis 57:2, pages 159-167.
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Goot Heah Khor, Gabrielle Ruth Anisah Froemming, Rosnah Binti Zain, Thomas Mannil Abraham & Thong Kwai Lin. (2016) Involvement of CELSR3 Hypermethylation in Primary Oral Squamous Cell Carcinoma. Asian Pacific Journal of Cancer Prevention 17:1, pages 219-223.
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Ahmad Hosseini Safa, Majid Fasihi Harandi, Mohammadhasan Tajaddini, Mohammad Rostami-Nejad, Mehdi Mohtashami-Pour & Nader Pestehchian. (2016) Rapid Identification of <i>Echinococcus granulosus</i> and <i>E. canadensis</i> Using High-Resolution Melting (HRM) Analysis by Focusing on a Single Nucleotide Polymorphism. Japanese Journal of Infectious Diseases 69:4, pages 300-305.
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Anton Dmitrievich Shirin & O.Yu. Baranova. (2016) Hypomethylating Agents in Oncohematology. Clinical oncohematology 9:4, pages 369-382.
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Guadalupe S. López-Álvarez, Tomasz K. Wojdacz, Claudia M. García-Cuellar, Hugo C. Monroy-Ramírez, Miguel A. Rodríguez Segura, Ruth A. Pacheco Rivera, Carlos A. Valencia-Antúnez, Nancy C. Cervantes-Anaya, Ernesto Soto-Reyes, Verónica R. Vázquez-Garzón, Yesennia Sánchez-Pérez & Saúl Villa Treviño. (2016) Gene silencing of Nox4 by CpG island methylation during hepatocarcinogenesis in rats . Biology Open.
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Tomasz Kazimierz Wojdacz. (2015) Biological and methodological aspects of assessment of locus specific de novo methylation in blood . Biomarkers in Medicine 9:12, pages 1291-1299.
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Melanie Spitzwieser, Elisabeth Holzweber, Georg Pfeiler, Stefan Hacker & Margit Cichna-Markl. (2015) Applicability of HIN-1, MGMT and RASSF1A promoter methylation as biomarkers for detecting field cancerization in breast cancer. Breast Cancer Research 17:1.
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Iben Daugaard, Tina E. Kjeldsen, Henrik Hager, Lise Lotte Hansen & Tomasz K. Wojdacz. (2015) The influence of DNA degradation in formalin-fixed, paraffin-embedded (FFPE) tissue on locus-specific methylation assessment by MS-HRM. Experimental and Molecular Pathology 99:3, pages 632-640.
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Jaeyun Yoon, Mi Kyoung Park, Tae Yoon Lee, Yong-Jin Yoon & Yong Shin. (2015) LoMA-B: a simple and versatile lab-on-a-chip system based on single-channel bisulfite conversion for DNA methylation analysis. Lab on a Chip 15:17, pages 3530-3539.
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Tomasz K Wojdacz, Johanne A Windeløv, Britta B Thestrup, Tine E Damsgaard, Jens Overgaard & Lise Lotte Hansen. (2014) Identification and characterization of locus-specific methylation patterns within novel loci undergoing hypermethylation during breast cancer pathogenesis. Breast Cancer Research 16:1.
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Yi-Zhen Lin & Po-Ling Chang. (2013) Colorimetric Determination of DNA Methylation Based on the Strength of the Hydrophobic Interactions between DNA and Gold Nanoparticles. ACS Applied Materials & Interfaces 5:22, pages 12045-12051.
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Huidan Zhang, Lianfeng Shan, Xiaonan Wang, Qian Ma & Jin Fang. (2013) A novel bisulfite-microfluidic temperature gradient capillary electrophoresis platform for highly sensitive detection of gene promotermethylation. Biosensors and Bioelectronics 42, pages 503-511.
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Gisela G.G. Slaats, Lovisa E. Reinius, Johan Alm, Juha Kere, Annika Scheynius & Maaike Joerink. (2012) DNA methylation levels within the CD 14 promoter region are lower in placentas of mothers living on a farm . Allergy 67:7, pages 895-903.
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Joshua Hihath, Shaoyin Guo, Peiming Zhang & Nongjian Tao. (2012) Effects of cytosine methylation on DNA charge transport. Journal of Physics: Condensed Matter 24:16, pages 164204.
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ME Ketelaar, RMW Hofstra & MR Hayden. (2011) What monozygotic twins discordant for phenotype illustrate about mechanisms influencing genetic forms of neurodegeneration. Clinical Genetics 81:4, pages 325-333.
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Rainer W. Fürst, Heinrich H.D. Meyer, Günther Schweizer & Susanne E. Ulbrich. (2012) Is DNA methylation an epigenetic contribution to transcriptional regulation of the bovine endometrium during the estrous cycle and early pregnancy?. Molecular and Cellular Endocrinology 348:1, pages 67-77.
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Tomasz K WojdaczBritta B Thestrup, Søren Cold, Jens Overgaard & Lise L Hansen. (2011) No difference in the frequency of locus-specific methylation in the peripheral blood DNA of women diagnosed with breast cancer and age-matched controls. Future Oncology 7:12, pages 1451-1455.
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M Yat Tse, Janet E Ashbury, Nora Zwingerman, Will D King, Sherry AM Taylor & Stephen C Pang. (2011) A refined, rapid and reproducible high resolution melt (HRM)-based method suitable for quantification of global LINE-1 repetitive element methylation. BMC Research Notes 4:1.
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Tomasz K Wojdacz, Britta B Thestrup, Jens Overgaard & Lise Lotte Hansen. (2011) Methylation of cancer related genes in tumor and peripheral blood DNA from the same breast cancer patient as two independent events. Diagnostic Pathology 6:1.
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Tatyana Azhikina, Alena Kozlova, Timofey Skvortsov & Eugene Sverdlov. (2011) Heterogeneity and degree of TIMP4, GATA4, SOX18, and EGFL7 gene promoter methylation in non–small cell lung cancer and surrounding tissues. Cancer Genetics 204:9, pages 492-500.
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