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BioTechniques Volume 25, 1998 - Issue 1

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Short Technical Reports

Making Genes Green: Creating Green Fluorescent Protein (GFP) Fusions with Blunt-End PCR Products

Wayne Lo1 Yale University School of Medicine, New Haven, CT, USA

William Rodgers1 Yale University School of Medicine, New Haven, CT, USA

Thomas Hughes1 Yale University School of Medicine, New Haven, CT, USACorrespondence[email protected]

Pages 94-98 | Received 07 Oct 1997, Accepted 25 Feb 1998, Published online: 15 Aug 2018

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https://doi.org/10.2144/98251st05

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Jennifer N. Byrum, Shuying Zhao, Negar S. Rahman, Lori M. Gwyn, William Rodgers & Karla K. Rodgers. (2015) An interdomain boundary in RAG1 facilitates cooperative binding to RAG2 in formation of the V(D)J recombinase complex. Protein Science 24:5, pages 861-873.
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Joy Leckie, Alexander Hope, Meghan Hughes, Sisir Debnath, Scott Fleming, Alastair W. Wark, Rein V. Ulijn & Mark D. Haw. (2014) Nanopropulsion by Biocatalytic Self-Assembly. ACS Nano 8:9, pages 9580-9589.
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Jennifer N. Byrum, Jeffrey S. Van Komen & William Rodgers. (2013) CD28 Sensitizes TCR Ca2+ Signaling during Ag-Independent Polarization of Plasma Membrane Rafts. The Journal of Immunology 191:6, pages 3073-3081.
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Bojing Shao, Tadayuki Yago, Phillip A. Coghill, Arkadiusz G. Klopocki, Padmaja Mehta-D'souza, David W. Schmidtke, William Rodgers & Rodger P. McEver. (2012) Signal-dependent Slow Leukocyte Rolling Does Not Require Cytoskeletal Anchorage of P-selectin Glycoprotein Ligand-1 (PSGL-1) or Integrin αLβ2. Journal of Biological Chemistry 287:23, pages 19585-19598.
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Gerald C. Kimbi, Michael C. Kew & Anna Kramvis. (2012) The effect of the G1888A mutation of subgenotype A1 of hepatitis B virus on the translation of the core protein. Virus Research 163:1, pages 334-340.
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Janeen L Arbuckle, Negar S Rahman, Shuying Zhao, William Rodgers & Karla K Rodgers. (2011) Elucidating the domain architecture and functions of non-core RAG1: The capacity of a non-core zinc-binding domain to function in nuclear import and nucleic acid binding. BMC Biochemistry 12:1.
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Gurunadh R. Chichili & William Rodgers. (2007) Clustering of Membrane Raft Proteins by the Actin Cytoskeleton. Journal of Biological Chemistry 282:50, pages 36682-36691.
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Jeffrey S. Van Komen, Sudha Mishra, Jennifer Byrum, Gurunadh R. Chichili, Jane C. Yaciuk, A. Darise Farris & William Rodgers. (2007) Early and Dynamic Polarization of T Cell Membrane Rafts and Constituents Prior to TCR Stop Signals. The Journal of Immunology 179:10, pages 6845-6855.
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Louisa Dowal, Paxton Provitera & Suzanne Scarlata. (2006) Stable Association between Gαq and Phospholipase Cβ1 in Living Cells. Journal of Biological Chemistry 281:33, pages 23999-24014.
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Thomas R. Hynes, Linnan Tang, Stacy M. Mervine, Jonathan L. Sabo, Evan A. Yost, Peter N. Devreotes & Catherine H. Berlot. (2004) Visualization of G Protein βγ Dimers Using Bimolecular Fluorescence Complementation Demonstrates Roles for Both β and γ in Subcellular Targeting. Journal of Biological Chemistry 279:29, pages 30279-30286.
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William Rodgers, Stephen J. Jordan & J. Donald Capra. (2002) Transient Association of Ku with Nuclear Substrates Characterized Using Fluorescence Photobleaching. The Journal of Immunology 168:5, pages 2348-2355.
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Anthony P. Sinai & Keith A. Joiner. (2001) The Toxoplasma gondii protein ROP2 mediates host organelle association with the parasitophorous vacuole membrane . The Journal of Cell Biology 154:1, pages 95-108.
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John E.J. Rasko. (2001) Reporters of Gene Expression: Autofluorescent Proteins. Current Protocols in Cytometry 7:1.
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Making Genes Green: Creating Green Fluorescent Protein (GFP) Fusions with Blunt-End PCR Products

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Making Genes Green: Creating Green Fluorescent Protein (GFP) Fusions with Blunt-End PCR Products

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