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BioTechniques Volume 25, 1998 - Issue 6

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PCR-Based Random Mutagenesis Method Using Spiked Oligonucleotides to Randomize Selected Parts of a Gene without any Wild-Type Background

Thomas Lanio1 Justus-Liebig Universität, Giessen, Germany

Albert Jeltsch1 Justus-Liebig Universität, Giessen, GermanyCorrespondence[email protected]

Pages 958-965 | Received 11 May 1998, Accepted 21 Aug 1998, Published online: 15 Aug 2018

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https://doi.org/10.2144/98256bm06

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Edson Cárcamo, Abigail Roldán-Salgado, Joel Osuna, Iván Bello-Sanmartin, Jorge A. Yáñez, Gloria Saab-Rincón, Héctor Viadiu & Paul Gaytán. (2017) Spiked Genes: A Method to Introduce Random Point Nucleotide Mutations Evenly throughout an Entire Gene Using a Complete Set of Spiked Oligonucleotides for the Assembly. ACS Omega 2:7, pages 3183-3191.
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Tamil Selvi Arunachalam, Claudia Wichert, Bettina Appel & Sabine Müller. (2012) Mixed oligonucleotides for random mutagenesis: best way of making them. Organic & Biomolecular Chemistry 10:24, pages 4641.
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Daryoush Abedi, Lin Zhang, Michael Pyne & C. Perry Chou. 2011. Comprehensive Biotechnology. Comprehensive Biotechnology 15 24 .

D. Abedi, L. Zhang, M. Pyne & C. Perry Chou. 2011. Comprehensive Biotechnology. Comprehensive Biotechnology 15 24 .

Thomas Lanio, Albert Jeltsch & Alfred Pingoud. 2008. Protein Science Encyclopedia. Protein Science Encyclopedia 309 327 .

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