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Original Article

Determination of Calcium Fractions of Serum. I. The Separation of Protein-bound and Protein-free Fractions by Means of a Simplified Ultrafiltration Technique

Pages 69-81 | Received 09 May 1969, Accepted 05 Jun 1969, Published online: 08 Jul 2009

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N. Fogh-Andersen, P. McNair, J. Møller-Petersen & S. Madsbad. (1983) Lowered serum ionized calcium in insulin treated diabetic subjects. Scandinavian Journal of Clinical and Laboratory Investigation 43:sup165, pages 93-97.
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Ole Siggaard-Andersen, Jørgen Thode & Niels Fogh-Andersen. (1983) What is “ionized calcium”?. Scandinavian Journal of Clinical and Laboratory Investigation 43:sup165, pages 11-16.
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H. S. Aabech & E. Steinnes. (1980) Determination of plasma inorganic iodine by neutron activation analysis after ultrafiltration. Scandinavian Journal of Clinical and Laboratory Investigation 40:6, pages 551-554.
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Kaj O. Pedersen. (1978) An analysis of measured and calculated calcium quantities in serum. Scandinavian Journal of Clinical and Laboratory Investigation 38:7, pages 659-667.
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K. O. Pedersen. (1972) On the Cause and Degree of Intraindividual Serum Calcium Variability. Scandinavian Journal of Clinical and Laboratory Investigation 30:2, pages 191-199.
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K. O. Pedersen. (1972) Binding of Calcium to Serum Albumin IV. Effect of Temperature and Thermodynamics of Calcium-Albumin Interaction. Scandinavian Journal of Clinical and Laboratory Investigation 30:1, pages 89-94.
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K. O. Pedersen. (1972) Binding of Calcium to Serum Albumin II. Effect of pH via Competitive Hydrogen and Calcium Ion Binding to the Imidazole Groups of Albumin. Scandinavian Journal of Clinical and Laboratory Investigation 29:1, pages 75-83.
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K. O. Pedersen. (1972) Binding of Calcium to Serum Albumin III. Influence of Ionic Strength and Ionic Medium. Scandinavian Journal of Clinical and Laboratory Investigation 29:4, pages 427-432.
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K. O. Pedersen. (1971) Binding of Calcium to Serum Albumin I. Stoichiometry and Intrinsic Association Constant at Physiological pH, Ionic Strength, and Temperature. Scandinavian Journal of Clinical and Laboratory Investigation 28:4, pages 459-469.
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K. O. Pedersen. (1971) Multiple Small Sample Ultrafiltration without Special Apparatus. Scandinavian Journal of Clinical and Laboratory Investigation 28:1, pages 57-59.
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K. O. Pedersen. (1971) The Effect of Bicarbonate, PCO2 and pH on Serum Calcium Fractions. Scandinavian Journal of Clinical and Laboratory Investigation 27:2, pages 145-150.
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K. O. Pedersen. (1971) On the Presence of Calcium Complexes in Aqueous Bicarbonate Solutions. Scandinavian Journal of Clinical and Laboratory Investigation 27:1, pages 9-13.
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K. O. Pedersen. (1970) Determination of Calcium Fractions of Serum. III. Ionized Calcium in Ultrafiltrates of Normal Serum and Examination of Pertinent Variables. Scandinavian Journal of Clinical and Laboratory Investigation 25:3, pages 223-230.
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Articles from other publishers (21)

J. Mariella, G. Isani, G. Andreani, F. Freccero, E. Carpenè & C. Castagnetti. (2016) Total plasma magnesium in healthy and critically ill foals. Theriogenology 85:2, pages 180-185.
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Ignacio Lopez, Jose C. Estepa, Francisco J. Mendoza, Mariano Rodriguez & Escolastico Aguilera-Tejero. (2006) Serum concentrations of calcium, phosphorus, magnesium and calciotropic hormones in donkeys. American Journal of Veterinary Research 67:8, pages 1333-1336.
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Ignacio Lopez, Jose C. Estepa, Francisco J. Mendoza, Rafael Mayer-Valor & Escolastico Aguilera-Tejero. (2006) Fractionation of calcium and magnesium in equine serum. American Journal of Veterinary Research 67:3, pages 463-466.
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Marilyn E. Morris & Inger M. Darling. (1990) Magnesium Cerebrospinal Fluid Concentrations and Protein Binding in Magnesium-Deficient Rats. Journal of Pharmaceutical Sciences 79:5, pages 458-459.
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B M Buckley & L J Russell. (2016) The Measurement of Ionised Calcium in Blood Plasma. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 25:5, pages 447-465.
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M. Peacock. 1988. Calcium in Human Biology. Calcium in Human Biology 125 169 .
R B Payne & D P Jones. (2016) Protein Interferes with Ionised Calcium Measurement at the Reference Electrode Liquid Junction. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 24:4, pages 400-407.
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J. H. KRISTIANSEN, J. BROCHNER MORTENSEN & K. O. PEDERSEN. (1985) FAMILIAL HYPOCALCIURIC HYPERCALCAEMIA I: RENAL HANDLING OF CALCIUM, MAGNESIUM AND PHOSPHATE. Clinical Endocrinology 22:1, pages 103-116.
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Jørgen Thode, Niels Fogh-Andersen, Mads Siggaard-Andersen & Ole Siggaard-Andersen. (2016) Donnan Effect or Protein Interference in Ionised Calcium Measurements?. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 20:5, pages 271-273.
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A. Raman & S.K. Lam. (1981) A simple device for ultrafiltration of serum under controlled pH. Clinical Biochemistry 14:3, pages 159-160.
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John B. Whitlam & Kenneth F. Brownx. (1981) Ultrafiltration in Serum Protein Binding Determinations. Journal of Pharmaceutical Sciences 70:2, pages 146-150.
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John Toffaletti & George N. BowersJr.Jr.. (1979) The possible physiologic importance of calcium complexes: An opinion. Clinica Chimica Acta 97:2-3, pages 101-105.
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Ulrich Trechsel, Otto Aeby & Herbert Fleisch. (1976) A new device for ultrafiltration The ultrafiltrability of calcium and inorganic phosphate in human serum. Clinica Chimica Acta 69:2, pages 367-373.
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Claus Christiansen, Jørgen Naestoft, Eigill F. Hvidberg, Niels-Erik Larsen & Birte Petersen. (1975) An easy procedure for in vivo estimation of protein binding and correction of elevated serum values induced by venous stasis. Clinica Chimica Acta 62:1, pages 65-71.
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M. L. RushtonH. G. SammonsP. GoslingB. H. B. Robinson. (2016) Measurement of Total, Ultrafilterable and Ionised Serum Calcium. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 10:1-6, pages 63-71.
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W. Kreusser, A. Heidland, H. Hennemann, M. E. Wigand & H. Knauf. (2008) Mono‐ and Divalent Electrolyte Patterns, pCO 2 and pH in Relation to Flow Rate in Normal Human Parotid Saliva* . European Journal of Clinical Investigation 2:6, pages 398-406.
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Edward R. Garrett, Josef Tsau & Peter H. Hinderling. (1972) Application of Ion-Pair Methods to Drug Extraction from Biological Fluids II: Quantitative Determination of Biguanides in Biological Fluids and Comparison of Protein Binding Estimates. Journal of Pharmaceutical Sciences 61:9, pages 1411-1418.
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O. Andersen. (1972) Ionized calcium in the effluent from ventriculo-cisternal perfusion in the rabbit. Experientia 28:7, pages 806-807.
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Janet M. Putman. (1972) A routine method for determining plasma ionised calcium and its application to the study of congenital heart disease in children. Clinica Chimica Acta 37, pages 33-41.
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LARS BERGMAN & BJÖRN ISAKSSON. (2008) PLASMA CALCIUM FRACTIONS IN NORMAL SUBJECTS FROM BIRTH TO ADULT AGES. Acta Paediatrica 60:6, pages 630-636.
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A. Raman. (1971) The calcium fractions of normal serum. Clinical Biochemistry 4:1-6, pages 141-146.
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