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Xenobiotica
the fate of foreign compounds in biological systems
Volume 16, 1986 - Issue 9
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Original Article

Critical evaluation of 7-ethoxycoumarin O-deethylase activity measurement in intact isolated rat hepatocytes

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Pages 817-826 | Received 10 Oct 1985, Published online: 30 Sep 2009

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ChristopherS. Mazur, JohnF. Kenneke, Caroline Tebes-Stevens, MilesS. Okino & JohnC. Lipscomb. (2007) In Vitro Metabolism of the Fungicide and Environmental Contaminant trans-bromuconazole and Implications for Risk Assessment. Journal of Toxicology and Environmental Health, Part A 70:14, pages 1241-1250.
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R. De Kanter, M. H. De Jager, A. L. Draaisma, J. U. Jurva, P. Olinga, D. K. F. Meijer & G. M. M. Groothuis. (2002) Drug-metabolizing activity of human and rat liver, lung, kidney and intestine slices. Xenobiotica 32:5, pages 349-362.
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D. M. Cross, J. A. Bell & K. Wilson. (1995) Kinetics of ranitidine metabolism in dog and rat isolated hepatocytes. Xenobiotica 25:4, pages 367-375.
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M. K. Bayliss, J. A. Bell, K. Wilson & G. R. Park. (1994) 7-Ethoxycoumarin O-deethylase kinetics in isolated rat, dog and human hepatocyte suspensions. Xenobiotica 24:3, pages 231-241.
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J. Barr, A. J. Weir, K. Brendel & I. G. Sipes. (1991) Liver slices in dynamic organ culture. I. An alternative in vitro technique for the study of rat hepatic drug metabolism. Xenobiotica 21:3, pages 331-339.
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L. A. P. Hoogenboom, F. J. H. Pastoor, W. E. Clous, S. E. Hesse & H. A. Kuiper. (1989) The use of porcine hepatocytes for biotransformation studies of veterinary drugs. Xenobiotica 19:11, pages 1207-1219.
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V. Rogiers, Y. Vandenberghe, A. Callaerts, W. Sonck, V. Maes & A. Vercruysse. (1988) The inducing and inhibiting effects of sodium valproate in vivo on the biotransformation systems of xenobiotics in isolated rat hepatocytes. Xenobiotica 18:6, pages 665-673.
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Mohd Sajid Ali & Hamad A. Al-Lohedan. (2018) Spectroscopic and computational evaluation on the binding of safranal with human serum albumin: Role of inner filter effect in fluorescence spectral correction. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 203, pages 434-442.
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Joana P. MirandaSofia B. LeiteUrsula Muller-VieiraArmanda RodriguesManuel J.T. CarrondoPaula M. Alves. (2009) Towards an Extended Functional Hepatocyte In Vitro Culture . Tissue Engineering Part C: Methods 15:2, pages 157-167.
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Christopher S. Mazur & John F. Kenneke. (2007) Cross-Species Comparison of Conazole Fungicide Metabolites Using Rat and Rainbow Trout (Onchorhynchus mykiss) Hepatic Microsomes and Purified Human CYP 3A4. Environmental Science & Technology 42:3, pages 947-954.
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D. Behera, A. Damre, A. Varghese & V. Addepalli. (2008) In Vitro Evaluation of Hepatic and Extra-hepatic Metabolism of Coumarins Using Rat Subcellular Fractions: Correlation of In Vitro Clearance with In Vivo Data. Drug Metabolism and Drug Interactions 23:3-4.
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Kouichi Minato, Naoyuki Koizumi, Seijirou Honma, Satoshi Iwamura & Kunio Tsukamoto. (1999) Characterizations of mouse hepatic microsomal monooxygenase catalyzing 11β-hydroxylation of osaterone acetate. Biochemical Pharmacology 58:2, pages 335-341.
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Georges de Sousa, Florence Nicolas, Michel Placidi, Roger Rahmani, Marc Benicourt, Bernard Vannier, Giocondo Lorenzon, Karine Mertens, Sandra Coecke, Andre Callaerts, Vera Rogiers, Shamas Khan, Phil Roberts, Paul Skett, Alain Fautrel, Christophe Chesne & Andre Guillouzo. (1999) A multi-laboratory evaluation of cryopreserved monkey hepatocyte functions for use in pharmaco–toxicology. Chemico-Biological Interactions 121:1, pages 77-97.
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Toshiro Niki, Krista Rombouts, Pieter De Bleser, Karen De Smet, Vera Rogiers, Detlef Schuppan, Minoru Yoshida, Giulio Gabbiani & Albert Geerts. (1999) A histone deacetylase inhibitor, trichostatin A, suppresses myofibroblastic differentiation of rat hepatic stellate cells in primary culture. Hepatology 29:3, pages 858-867.
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J.P Cravedi, E Perdu-Durand & A Paris. (1998) Cytochrome P450-dependent metabolic pathways and glucuronidation in trout liver slices. Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology 121:1-3, pages 267-275.
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K. de Smet, A. Callaerts, A. Vercruysse & V. Rogiers. (1997) Effect of phenobarbital on 7-Ethoxycoumarin-O-deethylase and microsomal epoxide hydrase activities in collagen gel cultures of rat hepatocytes. Toxicology in Vitro 11:5, pages 459-463.
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William R. WagnerDaniel J. MuzzioHoratio R. RiloTimothy DeglauMohammad M. AtaaiGeorge K. MichalopoulosGeoffrey D. Block. (1997) Effect of Growth Factors and Defined Medium on Primary Hepatocyte Culture on Polyester Carriers with Varying Surface Treatment. Tissue Engineering 3:3, pages 289-301.
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MARÍA JOSÉ GÓMEZ-LECHÓN, TERESA DONATO, XAVIER PONSODA, RICARDO FABRA, RAMÓN TRULLENQUE & JOSÉ V. CASTELL. 1997. In Vitro Methods in Pharmaceutical Research. In Vitro Methods in Pharmaceutical Research 129 153 .
K. Mertens, S. Kaufman, S. Waterschoot, A. Vercruysse & V. Rogiers. (1996) Effect of tertiary butylhydroperoxide-induced oxidative stress on glutathione content and thiobarbituric acid reactive substances production in cultures and co-cultures of adult rat hepatocytes. Toxicology in Vitro 10:4, pages 507-511.
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K. Mertens†A. Vercruysse†R. Rahmani, S. Kaufman§S. Waterschoot§V. Rogiers†. (1996) Interspecies differences in glutathione-dependent detoxication of hydroperoxides in short-term cultures of hepatocytes. Toxicology in Vitro 10:4, pages 473-478.
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Bas J. Blaauboer, Alan R. Boobis, Jose V. Castell, Sandra Coecke, Geny M.M. Groothuis, Andre Guillouzo, Tony J. Hall, Gabrielle M. Hawksworth, Giocondo Lorenzon, Herbert G. Miltenburger, Vera Rogiers, Paul Skett, Pia Villa & Friedrich J. Wiebel. (2020) The Practical Applicability of Hepatocyte Cultures in Routine Testing. Alternatives to Laboratory Animals 22:4, pages 231-241.
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Jeroen T.M. Buters, Claus D. Schiller & Ruby C. Chou. (1993) A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Biochemical Pharmacology 46:9, pages 1577-1584.
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Sandra Coecke, Karin Mertens, Anja Segaert, André Callaerts, Antoine Vercruysse & Vera Rogiers. (1992) Spectrophotometric measurement of flavin-containing monooxygenase activity in freshly isolated rat hepatocytes and their cultures. Analytical Biochemistry 205:2, pages 285-288.
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M. Teresa Donato, Jos� V. Castell & M. Jos� G�mez-Lech�n. (1992) A rapid and sensitive method for measuring monooxygenase activities in hepatocytes cultured in 96-well plates. Journal of Tissue Culture Methods 14:3, pages 153-157.
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V. Rogiers, M. Akrawi, Y. Vandenberghe, E. Shephard, I. Phillips & A. Vercruysse. 1992. Medical Toxicology. Medical Toxicology 156 159 .
Daniel W. Rosenberg, Henry Roque & Attallah Kappas. (1990) A fluorometric method for measuring ethoxycoumarin O-deethylase activity by reversed-phase high performance liquid chromatography. Analytical Biochemistry 191:2, pages 354-358.
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Vera Rogiers, Yves Vandenberghe, André Callaerts, Gino Verleye, Miranda Cornet, Karine Mertens, Walter Sonck & Antoine Vercruysse. (1990) Phase I and phase II xenobiotic biotransformation in cultures and co-cultures of adult rat hepatocytes. Biochemical Pharmacology 40:8, pages 1701-1706.
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V. Rogiers, Y. Vandenberghe, A. Callaerts, W. Sonck & A. Vercruysse. (1990) Effects of dimethylsulphoxide on phase I and II biotransformation in cultured rat hepatocytes. Toxicology in Vitro 4:4-5, pages 439-442.
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Ian N.H. White. (1988) A continuous fluorometric assay for cytochrome P-450-dependent mixed function oxidases using 3-cyano-7-ethoxycoumarin. Analytical Biochemistry 172:2, pages 304-310.
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John G. Deluca, Gary R. Dysart, David Rasnick & Matthews O. Bradley. (1988) A direct, highly sensitive assay for cytochrome P-450 catalyzed O-deethylation using a novel coumarin analog. Biochemical Pharmacology 37:9, pages 1731-1739.
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