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Xenobiotica
the fate of foreign compounds in biological systems
Volume 23, 1993 - Issue 12
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Research Article

Cryopreservation of human adult hepatocytes for use in drug metabolism and toxicity studies

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Pages 1399-1409 | Received 08 Jun 1993, Published online: 22 Sep 2008

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Ml Herrera-Herrera, R Zapata-Bustos & La Salazar-Olivo. (2009) Simplified culture techniques for growth and differentiation of murine and human pre-adipocytes for translational applications. Cytotherapy 11:1, pages 52-60.
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K. Tougou, H. Gotou, Y. Ohno & A. Nakamura. (2004) Stereoselective glucuronidation and hydroxylation of etodolac by UGT1A9 and CYP2C9 in man. Xenobiotica 34:5, pages 449-461.
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D. M. CROSS & M. K. BAYLISS. (2000) A COMMENTARY ON THE USE OF HEPATOCYTES IN DRUG METABOLISM STUDIES DURING DRUG DISCOVERY AND DEVELOPMENT*. Drug Metabolism Reviews 32:2, pages 219-240.
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Articles from other publishers (49)

Ruben M. F. Tomás, Robert Dallman, Thomas R. Congdon & Matthew I. Gibson. (2023) Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities. Biomaterials Science.
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Sang-Eun JungJin Seop AhnYong-Hee KimSeok-Man KimTea Gun UmBang-Jin KimBuom-Yong Ryu. (2021) Inhibition of Caspase-8 Activity Improves Freezing Efficiency of Male Germline Stem Cells in Mice. Biopreservation and Biobanking 19:6, pages 493-502.
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Sang-Eun Jung, Hui-Jo Oh, Jin-Seop Ahn, Yong-Hee Kim, Bang-Jin Kim & Buom-Yong Ryu. (2021) Antioxidant or Apoptosis Inhibitor Supplementation in Culture Media Improves Post-Thaw Recovery of Murine Spermatogonial Stem Cells. Antioxidants 10:5, pages 754.
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Mark D. Scott, Nobu Nakane & Elisabeth Maurer-Spurej. 2020. Cryopreservation - Current Advances and Evaluations. Cryopreservation - Current Advances and Evaluations.
Ming Zhou, Yayun Huang, Zhikui Cheng, Fei Zhao, Jiafu Li, Xiaoguang Zhi, Xiaohui Tian, Weihua Sun & Kanghong Hu. (2014) Revival, characterization, and hepatitis B virus infection of cryopreserved human fetal hepatocytes. Journal of Virological Methods 207, pages 29-37.
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Sonia Sentellas, Oriol Morales-Ibanez, Miriam Zanuy & Joan J. Albertí. (2014) GSSG/GSH ratios in cryopreserved rat and human hepatocytes as a biomarker for drug induced oxidative stress. Toxicology in Vitro 28:5, pages 1006-1015.
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Patricio Godoy, Nicola J. Hewitt, Ute Albrecht, Melvin E. Andersen, Nariman Ansari, Sudin Bhattacharya, Johannes Georg Bode, Jennifer Bolleyn, Christoph Borner, Jan Böttger, Albert Braeuning, Robert A. Budinsky, Britta Burkhardt, Neil R. Cameron, Giovanni Camussi, Chong-Su Cho, Yun-Jaie Choi, J. Craig Rowlands, Uta Dahmen, Georg Damm, Olaf Dirsch, María Teresa Donato, Jian Dong, Steven Dooley, Dirk Drasdo, Rowena Eakins, Karine Sá Ferreira, Valentina Fonsato, Joanna Fraczek, Rolf Gebhardt, Andrew Gibson, Matthias Glanemann, Chris E. P. Goldring, María José Gómez-Lechón, Geny M. M. Groothuis, Lena Gustavsson, Christelle Guyot, David Hallifax, Seddik Hammad, Adam Hayward, Dieter Häussinger, Claus Hellerbrand, Philip Hewitt, Stefan Hoehme, Hermann-Georg Holzhütter, J. Brian Houston, Jens Hrach, Kiyomi Ito, Hartmut Jaeschke, Verena Keitel, Jens M. Kelm, B. Kevin Park, Claus Kordes, Gerd A. Kullak-Ublick, Edward L. LeCluyse, Peng Lu, Jennifer Luebke-Wheeler, Anna Lutz, Daniel J. Maltman, Madlen Matz-Soja, Patrick McMullen, Irmgard Merfort, Simon Messner, Christoph Meyer, Jessica Mwinyi, Dean J. Naisbitt, Andreas K. Nussler, Peter Olinga, Francesco Pampaloni, Jingbo Pi, Linda Pluta, Stefan A. Przyborski, Anup Ramachandran, Vera Rogiers, Cliff Rowe, Celine Schelcher, Kathrin Schmich, Michael Schwarz, Bijay Singh, Ernst H. K. Stelzer, Bruno Stieger, Regina Stöber, Yuichi Sugiyama, Ciro Tetta, Wolfgang E. Thasler, Tamara Vanhaecke, Mathieu Vinken, Thomas S. Weiss, Agata Widera, Courtney G. Woods, Jinghai James Xu, Kathy M. Yarborough & Jan G. Hengstler. (2013) Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME. Archives of Toxicology 87:8, pages 1315-1530.
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J.-H. Lee, D.-H. Jung, D.-H. Lee, J.-K. Park & S.-K. Lee. (2012) Slow Cooling Rate with a Shock Cooling Program Can Effectively Cryopreserve Pig Hepatocytes. Transplantation Proceedings 44:4, pages 1002-1004.
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Jason H. Winnike, Peter Pediaditakis, Justyna E. Wolak, Randall E. McClelland, Paul B. Watkins & Jeffrey M. Macdonald. (2011) Stable isotope resolved metabolomics of primary human hepatocytes reveals a stressed phenotype. Metabolomics 8:1, pages 34-49.
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Ikuko Machida‐Sano, Yasushi Matsuda & Hideo Namiki. (2010) A novel harvesting method for cultured cells using iron‐cross‐linked alginate films as culture substrates. Biotechnology and Applied Biochemistry 55:1, pages 1-8.
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Liliana Chacón, Martha C. Gómez, Jill A. Jenkins, Stanley P. Leibo, Gemechu Wirtu, Betsy L. Dresser & C. Earle Pope. (2009) Production of bovine cloned embryos with donor cells frozen at a slow cooling rate in a conventional freezer (−20 °C). Zygote 17:4, pages 341-351.
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Mélanie Grondin, Francine Hamel, Diana A. Averill-Bates & Fathey Sarhan. (2009) Wheat Proteins Enhance Stability and Function of Adhesion Molecules in Cryopreserved Hepatocytes. Cell Transplantation 18:1, pages 79-88.
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Claire Terry & Robin D. Hughes. 2009. Hepatocyte Transplantation. Hepatocyte Transplantation 25 34 .
Mélanie Grondin, Francine Hamel, Fathey Sarhan & Diana A. Averill-Bates. (2008) Metabolic Activity of Cytochrome P450 Isoforms in Hepatocytes Cryopreserved with Wheat Protein Extract. Drug Metabolism and Disposition 36:10, pages 2121-2129.
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J. Akhter, L.A. Johnson, A. Gunasegaram, S.M. Riordan & D.L. Morris. (2007) Hepatocyte transplantation: A review of laboratory techniques and clinical experiences. The Surgeon 5:3, pages 155-164.
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Claire Terry, Anil Dhawan, Ragai R. Mitry & Robin D. Hughes. (2006) Cryopreservation of isolated human hepatocytes for transplantation: State of the art. Cryobiology 53:2, pages 149-159.
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Masayoshi Abe, Shiro Jimi, Hiroshi Hama, Takeshi Shiraishi, Akinori Iwasaki, Nobuhumi Ono, Takayuki Shirakusa & Takeshi Katsuragi. (2006) A Novel Method for Preserving Human Lungs Using a Super-Cooling System. The Annals of Thoracic Surgery 82:3, pages 1085-1088.
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N. Hariparsad, R.S. Sane, S.C. Strom & P.B. Desai. (2006) In vitro methods in human drug biotransformation research: Implications for cancer chemotherapy. Toxicology in Vitro 20:2, pages 135-153.
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Claire Terry, Ragai R. Mitry, Sharon C. Lehec, Paulo Muiesan, Mohamed Rela, Nigel D. Heaton, Robin D. Hughes & Anil Dhawan. (2017) The Effects of Cryopreservation on Human Hepatocytes Obtained from Different Sources of Liver Tissue. Cell Transplantation 14:8, pages 585-594.
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Natalie Z.M. Homer, John Reglinski, Rebecca Sowden, Corinne M. Spickett, Rhoda Wilson & James J. Walker. (2005) Dimethylsulfoxide oxidizes glutathione in vitro and in human erythrocytes: Kinetic analysis by 1H NMR. Cryobiology 50:3, pages 317-324.
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M.H. Grant & D. Stevenson. 2005. Bioreactors for Tissue Engineering. Bioreactors for Tissue Engineering 353 372 .
D.J. Stevenson, C. Morgan, E. Goldie, G. Connel & M.H. Grant. (2004) Cryopreservation of viable hepatocyte monolayers in cryoprotectant media with high serum content: metabolism of testosterone and kaempherol post-cryopreservation. Cryobiology 49:2, pages 97-113.
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Graham Somers, Peter Mutch & Amanda Woodrooffe. 2004. A Handbook of Bioanalysis and Drug Metabolism. A Handbook of Bioanalysis and Drug Metabolism.
Tom D.R. Lloyd, Samantha Orr & Ashley R. Dennison. (2003) A Survey of Consumer Attitudes to the Supply and Use of Human Hepatocytes in the United Kingdom. Alternatives to Laboratory Animals 31:5, pages 483-488.
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Yoichi Naritomi, Shigeyuki Terashita, Akira Kagayama & Yuichi Sugiyama. (2003) Utility of Hepatocytes in Predicting Drug Metabolism: Comparison of Hepatic Intrinsic Clearance in Rats and Humans in Vivo and in Vitro. Drug Metabolism and Disposition 31:5, pages 580-588.
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T Iwahori. (2003) CYP3A4 inducible model for in vitro analysis of human drug metabolism using a bioartificial liver. Hepatology 37:3, pages 665-673.
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Christopher Stroh, Uwe Cassens, Ajoy K. Samraj, Walter Sibrowski, Klaus Schulze‐Osthoff & Marek Los. (2002) The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells. The FASEB Journal 16:12, pages 1651-1653.
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E Alexandre, C Viollon-Abadie, P David, A Gandillet, P Coassolo, B Heyd, G Mantion, P Wolf, P Bachellier, D Jaeck & L Richert. (2002) Cryopreservation of adult human hepatocytes obtained from resected liver biopsies. Cryobiology 44:2, pages 103-113.
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G.C McKay, C Henderson, E Goldie, G Connel, C Westmoreland & M.H Grant. (2002) Cryopreservation of rat hepatocyte monolayers: cell viability and cytochrome P450 content in post-thaw cultures. Toxicology in Vitro 16:1, pages 71-79.
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