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Xenobiotica
the fate of foreign compounds in biological systems
Volume 24, 1994 - Issue 7
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Original Article

Use of organotypical cultures of primary hepatocytes to analyse drug biotransformation in man and animals

A. Bader Institute of General Pharmacology Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
,
K. Zech Byk Gulden, Byk-Gulden Str. 2, 78467, Konstanz, Germany
,
O. Crome Institute of General Pharmacology Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
,
U. Christians Institute of General Pharmacology Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
,
B. Ringe Hospital for Abdominal and Transplant Surgery, Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
,
R. Pichlmayr Hospital for Abdominal and Transplant Surgery, Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
&
K-Fr. Sewing Institute of General Pharmacology Medical School of Hannover (MHH), Konstanty-Gutschow-Str. 8, 30625, Hannover, Germany
 show all
Pages 623-633 | Received 11 Nov 1993, Published online: 27 Aug 2009

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Ali Acikgöz, Shibashish Giri & Augustinus Bader. (2012) Detection of nanolevel drug metabolites in an organotypic culture of primary human hepatocytes and porcine hepatocytes with special reference to a two-compartment model. International Journal of Nanomedicine 7, pages 5859-5872.
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Shibashish Giri, Karen Nieber & Augustinus Bader. (2010) Hepatotoxicity and hepatic metabolism of available drugs: current problems and possible solutions in preclinical stages. Expert Opinion on Drug Metabolism & Toxicology 6:8, pages 895-917.
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E. Schmelzer, A. Acikgoez, N. R. Frühauf, O. Crome, J. Klempnauer, U. Christians & A. Bader. (2006) Biotransformation of cyclosporin in primary rat, porcine and human liver cell co-cultures. Xenobiotica 36:8, pages 693-708.
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D. M. CROSS & M. K. BAYLISS. (2000) A COMMENTARY ON THE USE OF HEPATOCYTES IN DRUG METABOLISM STUDIES DURING DRUG DISCOVERY AND DEVELOPMENT*. Drug Metabolism Reviews 32:2, pages 219-240.
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U. G. Sidelmann, C. Cornett, J. Tjørnelund & S. H. Hansen. (1996) A comparative study of precision cut liver slices, hepatocytes, and liver microsomes from the Wistar rat using metronidazole as a model substance. Xenobiotica 26:7, pages 709-722.
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S. A. Pahernik, J. Schmid, T. Sauter, F. W. Schildberg & H.-G. Koebe. (1995) Metabolism of pimobendan in long-term human hepatocyte culture: in vivo-in vitro comparison. Xenobiotica 25:8, pages 811-823.
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Ali Acikgöz, Shibashish Giri, Man-Gi Cho & Augustinus Bader. (2013) Morphological and Functional Analysis of Hepatocyte Spheroids Generated on Poly-HEMA-Treated Surfaces under the Influence of Fetal Calf Serum and Nonparenchymal Cells. Biomolecules 3:4, pages 242-269.
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Tatsuyuki Kanamori, Kenji Kuwayama, Kenji Tsujikawa, Hajime Miyaguchi, Yuko Togawa-Iwata & Hiroyuki Inoue. (2011) A model system for prediction of the in vivo metabolism of designer drugs using three-dimensional culture of rat and human hepatocytes. Forensic Toxicology 29:2, pages 142-151.
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Michael Maringka, Shibashish Giri, Karen Nieber, Ali Acikgöz & Augustinus Bader. (2011) Biotransformation of diazepam in a clinically relevant flat membrane bioreactor model using primary porcine hepatocytes. Fundamental & Clinical Pharmacology 25:3, pages 343-353.
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CHUKWUEMEKA ANENE-NZELU, YAN WANG, HANRY YU & LEO HWA LIANG. (2011) LIVER TISSUE MODEL FOR DRUG TOXICITY SCREENING. Journal of Mechanics in Medicine and Biology 11:02, pages 369-390.
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Jan-Henning Frühauf, Heike Mertsching, Shibashish Giri, Nils Roman Frühauf & Augustinus Bader. (2009) Porcine endogenous retrovirus released by a bioartificial liver infects primary human cells. Liver International 29:10, pages 1553-1561.
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F. Heidebrecht, I. Schulz, M. Keller, S.-E. Behrens & A. Bader. (2009) Improved protocols for protein and RNA isolation from three-dimensional collagen sandwich cultures of primary hepatocytes. Analytical Biochemistry 393:1, pages 141-144.
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Angelika Langsch, Shibashish Giri, Ali Acikgöz, Inka Jasmund, Bernd Frericks & Augustinus Bader. (2009) Interspecies difference in liver-specific functions and biotransformation of testosterone of primary rat, porcine and human hepatocyte in an organotypical sandwich culture. Toxicology Letters 188:3, pages 173-179.
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Ali Acikgöz, Najibulla Karim, Shibashish Giri, Wolfgang Schmidt-Heck & Augustinus Bader. (2009) Two compartment model of diazepam biotransformation in an organotypical culture of primary human hepatocytes. Toxicology and Applied Pharmacology 234:2, pages 179-191.
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Margarita KataropoulouCatherine HendersonM. Helen Grant. (2005) Metabolic Studies of Hepatocytes Cultured on Collagen Substrata Modified to Contain Glycosaminoglycans. Tissue Engineering 11:7-8, pages 1263-1273.
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SHIGEKI MIYAMOTO, KOICHI HIRATA, SHINICHI SUGIMOTO, KEISUKE HARADA & TOSHIHIRO MITAKA. (2005) Expression of cytochrome P450 enzymes in hepatic organoid reconstructed by rat small hepatocytes. Journal of Gastroenterology and Hepatology 20:6, pages 865-872.
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Dora Farkas & Steven R. Tannenbaum. (2005) Characterization of Chemically Induced Hepatotoxicity in Collagen Sandwiches of Rat Hepatocytes. Toxicological Sciences 85:2, pages 927-934.
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T. Kanamori, K. Tsujikawa, Y. Ohmae, Y.T. Iwata, H. Inoue, T. Kishi, T. Nakahama & Y. Inouye. (2005) A study of the metabolism of methamphetamine and 4-bromo-2,5-dimethoxyphenethylamine (2C-B) in isolated rat hepatocytes. Forensic Science International 148:2-3, pages 131-137.
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Stefan Wilkening, Frank Stahl & Augustinus Bader. (2003) COMPARISON OF PRIMARY HUMAN HEPATOCYTES AND HEPATOMA CELL LINE HEPG2 WITH REGARD TO THEIR BIOTRANSFORMATION PROPERTIES. Drug Metabolism and Disposition 31:8, pages 1035-1042.
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Stefan Wilkening & Augustinus Bader. (2003) Influence of culture time on the expression of drug‐metabolizing enzymes in primary human hepatocytes and hepatoma cell line HepG2. Journal of Biochemical and Molecular Toxicology 17:4, pages 207-213.
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Margarita Kataropoulou, Catherine Henderson & Helen Grant. (2016) The influence of glycosaminoglycans and crosslinking agents on the phenotype of hepatocytes cultured on collagen gels. Human & Experimental Toxicology 22:2, pages 65-71.
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Anna M. Wobus. 2003. Grundlagen der Molekularen Medizin. Grundlagen der Molekularen Medizin 255 298 .
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Katrien Slaus, Michael W.H Coughtrie, Sheila Sharp, Tamara Vanhaecke, Antoine Vercruysse & Vera Rogiers. (2001) Influence of culture system and medium enrichment on sulfotransferase and sulfatase expression in male rat hepatocyte cultures. Biochemical Pharmacology 61:9, pages 1107-1117.
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M I Grant, K Anderson, G McKay, M Wills, C Henderson & C MacDonald. (2016) Manipulation of the phenotype of immortalised rat hepatocytes by different culture configurations and by dimethyl sulphoxide. Human & Experimental Toxicology 19:5, pages 309-317.
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Martin K. Bayliss & David M. Cross. 2000. The Hepatocyte Review. The Hepatocyte Review 365 389 .
Maria José Gómez-Lechón, Ramiro Jover, Teresa Donato, Xavier Ponsoda & José V. Castell. 2000. Signaling Through the Cell Matrix. Signaling Through the Cell Matrix 89 104 .
K. De Smet, S. Beken, M. Depreter, F. Roels, A. Vercruysse & V. Rogiers. (1999) Effect of Epidermal Growth Factor in Collagen Gel Cultures of Rat Hepatocytes. Toxicology in Vitro 13:4-5, pages 579-585.
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Sandra CoeckeVera RogiersMartin BaylissJosé CastellJohannes DoehmerGérard FabreJeffrey FryArmin Kern & Carl Westmoreland. (2019) The use of Long-term Hepatocyte Cultures for Detecting Induction of Drug Metabolising Enzymes: The Current Status. Alternatives to Laboratory Animals 27:4, pages 579-638.
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BENEDIKT BUSSE & JÖRG C. GERLACH. (2006) Bioreactors for Hybrid Liver Support: Historical Aspects and Novel Designs. Annals of the New York Academy of Sciences 875:1, pages 326-339.
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H Hildebrand, U Schmidt, G Kempka, R Jacob, H.J Ahr, C Ebener, P.E Goretzki & A Bader. (1999) An in vitro model for peroxisome proliferation utilizing primary hepatocytes in sandwich culture. Toxicology in Vitro 13:2, pages 265-273.
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A. Bader, N. Fr?hauf, M. Tiedge, M. Drinkgern, L. De Bartolo, J.T. Borlak, G. Steinhoff & A. Haverich. (1999) Enhanced Oxygen Delivery Reverses Anaerobic Metabolic States in Prolonged Sandwich Rat Hepatocyte Culture. Experimental Cell Research 246:1, pages 221-232.
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R Fahrig, M Rupp, A Steinkamp-Zucht & A Bader. (1998) Use of Primary Rat and Human Hepatocyte Sandwich Cultures for Activation of Indirect Carcinogens: Monitoring of DNA Strand Breaks and Gene Mutations in Co-cultured Cells. Toxicology in Vitro 12:4, pages 431-444.
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Xavier Ponsoda, Maria Jose Gómez-Lechón & Jose V. Castell. (2020) Toxicity and Cell Density Monitoring in Monolayer and Three-dimensional Cultures with the XTT Assay. Alternatives to Laboratory Animals 26:3, pages 331-342.
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K Anderson, R Andrews, L Yin, R McLeod, C MacDonald, J D Hayes & M H Grant. (2016) Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalised rat hepatocyte cell lines. Human & Experimental Toxicology 17:3, pages 131-137.
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S. Beken, T. Tytgat, S. Pahernik, H.-G. Koebe, A. Vercruysse & V. Rogiers. (1997) Cell morphology, albumin secretion and glutathione S-Transferase expression in collagen gel sandwich and immobilization cultures of rat hepatocytes. Toxicology in Vitro 11:5, pages 409-416.
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A Kern, A Bader, R Pichlmayr & K.-F Sewing. (1997) Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochemical Pharmacology 54:7, pages 761-772.
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Carole Lerche, Alain Fautrel, Peter M. Shaw, Denise Glaise, Francois Ballet, Andre Guillouzo & Laurent Corcos. (2004) Regulation of the Major Detoxication Functions by Phenobarbital and 3‐Methylcholanthrene in Co‐Cultures of Rat Hepatocytes and Liver Epithelial Cells. European Journal of Biochemistry 244:1, pages 98-106.
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P. Olinga, D.K.F. Meijer, M.J.H. Slooff & G.M.M. Groothuis. (1997) Liver slices in in vitro pharmacotoxicology with special reference to the use of human liver tissue. Toxicology in Vitro 12:1, pages 77-100.
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A Bader, P Reimer, E Knop, K Böker, U Christians, R Weissleder & K.-Fr Sewing. (1995) An organotypical in vitro model of the liver parenchyma for uptake studies of diagnostic MR receptor agents. Magnetic Resonance Imaging 13:7, pages 991-1002.
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