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Current Eye Research Volume 38, 2013 - Issue 5
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Research Article

Comparative Evaluation of Uniplex, Nested, Semi-nested, Multiplex and Nested Multiplex PCR Methods in the Identification of Microbial Etiology of Clinically Suspected Infectious Endophthalmitis

Madasamy Jayahar BharathiDepartment of Microbiology & Molecular BiologyCorrespondence[email protected] [email protected]
,
Gunasekaran RameshkumarDepartment of Microbiology & Molecular Biology
,
Rengappa RamakrishnanDepartment of Glaucoma
,
Yerahaia Chinna Venugopal ReddyDepartment of Retina & Vitreous
,
Chandrasekar ShivkumarDepartment of Cataract and IOL, Aravind Eye Hospital & Postgraduate Institute of Ophthalmology SN High Road, Tirunelveli 627001, Tamil NaduIndia
&
Srinivasan RameshPostgraduate Department of Microbiology, Sri Paramakalyani College Alwarkuruchi, Tiruneveli 627412, Tamil NaduIndia
Pages 550-562 | Received 10 Sep 2012, Accepted 27 Jan 2013, Published online: 28 Feb 2013

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Christine L. Tan, Harsha Sheorey, Penelope J. Allen & Rosie C. H. Dawkins. (2023) Endophthalmitis: Microbiology and Organism Identification Using Current and Emerging Techniques. Ocular Immunology and Inflammation 31:2, pages 393-401.
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Harpal Singh Sandhu, Amir Hajrasouliha, Henry J. Kaplan & Wei Wang. (2019) Diagnostic Utility of Quantitative Polymerase Chain Reaction versus Culture in Endophthalmitis and Uveitis. Ocular Immunology and Inflammation 27:4, pages 578-582.
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Stephen G Schwartz & Harry W Flynn Jr. (2014) Update on the prevention and treatment of endophthalmitis. Expert Review of Ophthalmology 9:5, pages 425-430.
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Articles from other publishers (20)

Taraprasad Das, Joveeta Joseph, Matthew P. Simunovic, Andrzej Grzybowski, Kuan-Jen Chen, Vivek Pravin Dave, Savitri Sharma, Patrick Staropoli & Harry FlynnJr.Jr.. (2023) Consensus and controversies in the science of endophthalmitis management: Basic research and clinical perspectives. Progress in Retinal and Eye Research 97, pages 101218.
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Xuejie Li, Ziyue Li, Ming Wang, Aisi Fu, Xinlei Hao, Xinyang Guo, Jiashuang Gu, Wei Jin & Anhuai Yang. (2023) The diagnostic utility of nanopore targeted sequencing in suspected endophthalmitis. International Ophthalmology 43:8, pages 2653-2668.
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Nathaniel S. Ashby, Taylor J. Johnson, Yasmyne Castillo-Ronquillo, Carter J. Payne, Connor Davenport, Phillip C. Hoopes & Majid Moshirfar. (2023) Cutibacterium (Formerly Propionibacterium) acnes Keratitis: A Review. Eye & Contact Lens: Science & Clinical Practice Publish Ahead of Print.
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Christophe Chiquet, Alain M. Bron, Mats Lundström & Max Maurin. (2022) Acute postoperative endophthalmitis: Microbiology from the laboratory to the bedside. Survey of Ophthalmology 67:6, pages 1698-1710.
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Laurel T. Tainsh, Marisa G. Tieger & David M. Wu. (2022) Propionibacterium acnes–associated Delayed-onset Pseudophakic Endophthalmitis. International Ophthalmology Clinics 62:2, pages 145-156.
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Frederick C. Miller, Phillip S. Coburn, Mursalin Md Huzzatul, Austin L. LaGrow, Erin Livingston & Michelle C. Callegan. (2019) Targets of immunomodulation in bacterial endophthalmitis. Progress in Retinal and Eye Research 73, pages 100763.
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Fan Chen, Lei Liu, Qili He, Yan Huang, Wentao Wang, Guo Zhou, Wenjie Yu, Wei He, Qi Wang, Guangjia Zhang, Sha Liao, Ruirui Li, Liu Yang, Renxin Yao, Qian Wang & Bo Zhong. (2019) A multiplex PCR for the identification of Echinococcus multilocularis , E. granulosus sensu stricto and E. canadensis that infect human . Parasitology 146:12, pages 1595-1601.
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Yichen Tian, Lefei Wang, Jinyang Zhang, Qinqin Han, Xue-shan Xia, Yuzhu Song & Guangying Yang. (2019) CelB is a suitable marker for rapid and specific identification of Klebsiella pneumoniae by the loop-mediated isothermal amplification (LAMP) assay. Brazilian Journal of Microbiology 50:4, pages 961-967.
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Chao Li, Gongyu Fu, Yaoqiang Shi, A-Mei Zhang, Xueshan Xia, Yue Fang, Xiaoqin Mao, Jie Jiang, Yuzhu Song & Guangying Yang. (2019) Rapid, specific, and sensitive detection of the ureR_1 gene in Klebsiella pneumoniae by loop-mediated isothermal amplification method. Brazilian Journal of Medical and Biological Research 52:3.
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Deepanshi Mishra, Gita Satpathy, Rohan Chawla, Pradeep Venkatesh, Nishat Hussain Ahmed & Subrat Kumar Panda. (2019) Utility of broad-range 16S rRNA PCR assay versus conventional methods for laboratory diagnosis of bacterial endophthalmitis in a tertiary care hospital. British Journal of Ophthalmology 103:1, pages 152-156.
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Saumitra Sen Singh, Sachchida Nand Rai, Hareram Birla, Walia Zahra, Aaina Singh Rathore, Hagera Dilnashin, Chetan Keswani & Surya Pratap Singh. 2019. Application of Biomedical Engineering in Neuroscience. Application of Biomedical Engineering in Neuroscience 437 456 .
Daisuke Sora, Kei Takayama, Manzo Taguchi, Tomohito Sato, Yutaka Sakurai, Takayuki Kanda & Masaru Takeuchi. (2018) Topical Corticosteroid-Resolved Rubeosis Iridis with Neovascular Glaucoma Caused by Noninfectious Granulomatous Uveitis. Case Reports in Ophthalmology 9:1, pages 243-247.
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Savitri Sharma. 2018. Endophthalmitis. Endophthalmitis 293 298 .
David E. KuoMaggie M. WeiKaren R. ArmbrustJared E. KnickelbeinIan Y.L. YeungRobert B. NussenblattChi-Chao ChanHatice Nida Sen. (2017) Gradient Boosted Decision Tree Classification of Endophthalmitis Versus Uveitis and Lymphoma from Aqueous and Vitreous IL-6 and IL-10 Levels. Journal of Ocular Pharmacology and Therapeutics 33:4, pages 319-324.
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Cuiming Cao, Fang Zhang, Mingyu Ji, Fengyan Pei, Xiujie Fan, Hong Shen, Qingxi Wang, Weihua Yang & Yunshan Wang. (2016) Development of a loop-mediated isothermal amplification method for rapid detection of streptococcal pyrogenic exotoxin B. Toxicon 117, pages 53-58.
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Christophe Chiquet, Sandrine Boisset, Pierre-Loïc Cornut & Max Maurin. 2016. Endophthalmitis. Endophthalmitis 77 97 .
Bryan K. Hong, Cecilia S. Lee, Russell N. Van Gelder & Sunir J. Garg. (2015) Emerging techniques for pathogen discovery in endophthalmitis. Current Opinion in Ophthalmology 26:3, pages 221-225.
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K Liu, F Fang & H Li. (2014) Reliability of vitreous histological detection of pathogenic fungi in the diagnosis of fungal endophthalmitis. Eye 29:3, pages 424-427.
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Aaron Y. Lee, Lakshmi Akileswaran, Michael D. Tibbetts, Sunir J. Garg & Russell N. Van Gelder. (2015) Identification of Torque Teno Virus in Culture-Negative Endophthalmitis by Representational Deep DNA Sequencing. Ophthalmology 122:3, pages 524-530.
Crossref
Jolanda D. F. de Groot-Mijnes. 2014. Ocular Infections. Ocular Infections 1 18 .

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