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Current Eye Research Volume 11, 1992 - Issue 7
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Original Article

Two-dimensional gel electrophoretic analysis of human lens proteins

Manuel B. Datiles National Eye Institute, National Institutes of Health, Bethesda, MD, USA
,
D. James Schumer Henry Ford Hospital, Department of Ophthalmology, Detroit, MI, USA
,
J. Samuel Zigler National Eye Institute, National Institutes of Health, Bethesda, MD, USA
,
Paul Russell National Eye Institute, National Institutes of Health, Bethesda, MD, USA
,
Leigh Anderson Large Scale Biology Corporation, Rockville, MD, USA
&
Donita Garland National Eye Institute, National Institutes of Health, Bethesda, MD, USA
Pages 669-677 | Received 10 Feb 1992, Accepted 19 Jun 1992, Published online: 02 Jul 2009

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Thomas C Lam, Rachel Km Chun, King‐kit Li & Chi‐ho To. (2008) Application of proteomic technology in eye research: a mini review. Clinical and Experimental Optometry 91:1, pages 23-33.
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Articles from other publishers (23)

Z. Kyselova. (2010) Mass spectrometry‐based proteomics approaches applied in cataract research. Mass Spectrometry Reviews 30:6, pages 1173-1184.
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Michael Ahrend, Angela Käberich, Marie-Therese Fergen & Brigitte Schmitz. 2008. Post-translational Modifi cations of Proteins. Post-translational Modifi cations of Proteins 267 280 .
W. Hoehenwarter, J. Klose & P. R. Jungblut. (2006) Eye lens proteomics. Amino Acids 30:4, pages 369-389.
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Daniel Organisciak, Ruth Darrow, Xiaorong Gu, Linda Barsalou & John W. Crabb. (2006) Genetic, Age and Light Mediated Effects on Crystallin Protein Expression in the Retina. Photochemistry and Photobiology 82:4, pages 1088.
Crossref
Paul C. Guest, Heather A. Skynner, Kamran Salim, F. David Tattersall, Michael R. Knowles & John R. Atack. (2006) Detection of gender differences in rat lens proteins using 2-D-DIGE. PROTEOMICS 6:2, pages 667-676.
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Mark E. Ireland. (2005) Activated epidermal growth factor receptors in the adult human lens. Experimental Eye Research 80:3, pages 443-445.
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Veniamin N. Lapko, Ronald L. Cerny, David L. Smith & Jean B. Smith. (2009) Modifications of human βA1/βA3-crystallins include S-methylation, glutathiolation, and truncation. Protein Science 14:1, pages 45-54.
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Mark E. Ireland & Linda K. Mrock. (2004) Expression and activation of the epidermal growth factor receptor in differentiating cells of the developing and post-hatching chicken lens. Experimental Eye Research 79:3, pages 305-312.
Crossref
Zhongli Zhang, David L. Smith & Jean B. Smith. (2003) Human β-crystallins modified by backbone cleavage, deamidation and oxidation are prone to associate. Experimental Eye Research 77:3, pages 259-272.
Crossref
Veniamin N. Lapko, David L. Smith & Jean B. Smith. (2003) Methylation and carbamylation of human γ-crystallins. Protein Science 12:8, pages 1762-1774.
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H Thomas SteelyJr.Jr. & Abbot F Clark. (2000) The use of proteomics in ophthalmic research. Pharmacogenomics 1:3, pages 267-280.
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Stacy R.A Hanson, Azeem Hasan, David L Smith & Jean B Smith. (2000) The Major in vivo Modifications of the Human Water-insoluble Lens Crystallins are Disulfide Bonds, Deamidation, Methionine Oxidation and Backbone Cleavage. Experimental Eye Research 71:2, pages 195-207.
Crossref
P.R Jungblut, A Otto, J Favor, M Löwe, E.-C Müller, M Kastner, K Sperling & J Klose. (1998) Identification of mouse crystallins in 2D protein patterns by sequencing and mass spectrometry. Application to cataract mutants. FEBS Letters 435:2-3, pages 131-137.
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STACY R.A. HANSON, DAVID L. SMITH & JEAN B. SMITH. (1998) Deamidation and Disulfide Bonding in Human Lens γ-Crystallins. Experimental Eye Research 67:3, pages 301-312.
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ZHIXIANG MA, STACY R.A. HANSON, KIRSTEN J. LAMPI, LARRY L. DAVID, DAVID L. SMITH & JEAN B. SMITH. (1998) Age-Related Changes in Human Lens Crystallins Identified by HPLC and Mass Spectrometry. Experimental Eye Research 67:1, pages 21-30.
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KIRSTEN J. LAMPI, ZHIXIANG MA, STACY R.A. HANSON, MISUYOSHI AZUMA, MARJORIE SHIH, THOMAS R. SHEARER, DAVID L. SMITH, JEAN B. SMITH & LARRY L. DAVID. (1998) Age-related Changes in Human Lens Crystallins Identified by Two-dimensional Electrophoresis and Mass Spectrometry. Experimental Eye Research 67:1, pages 31-43.
Crossref
Peiping P. Lin, Richard C. Barry, David L. Smith & Jean B. Smith. (2008) In vivo acetylation identified at lysine 70 of human lens αA‐crystallin. Protein Science 7:6, pages 1451-1457.
Crossref
PAULINE T. VELASCO, THOMAS J. LUKAS, S.N.PRASANNA MURTHY, YVONNE DUGLAS-TABOR, DONITA L. GARLAND & LASZLO LORAND. (1997) Hierarchy of Lens Proteins Requiring Protection Against Heat-Induced Precipitation by the α Crystallin Chaperone. Experimental Eye Research 65:4, pages 497-505.
Crossref
Tian-Xiao Sun, Biplab K. Das & Jack J.-N. Liang. (1997) Conformational and Functional Differences between Recombinant Human Lens αA- and αB-Crystallin. Journal of Biological Chemistry 272:10, pages 6220-6225.
Crossref
Kirsten J. Lampi, Zhixiang Ma, Marjorie Shih, Thomas R. Shearer, Jean B. Smith, David L. Smith & Larry L. David. (1997) Sequence Analysis of βA3, βB3, and βA4 Crystallins Completes the Identification of the Major Proteins in Young Human Lens. Journal of Biological Chemistry 272:4, pages 2268-2275.
Crossref
Larry L. David, Kirsten J. Lampi, Anders L. Lund & Jean B. Smith. (1996) The Sequence of Human βB1-Crystallin cDNA Allows Mass Spectrometric Detection of βB1 Protein Missing Portions of Its N-terminal Extension. Journal of Biological Chemistry 271:8, pages 4273-4279.
Crossref
Shuming He, Suhua Pan, Kaili Wu, I. Jonathan Amster & Ron Orlando. (2005) Analysis of normal human fetal eye lens crystallins by high‐performance liquid chromatography/mass spectrometry. Journal of Mass Spectrometry 30:3, pages 424-431.
Crossref
Larry L. David & Thomas R. Shearer. (1993) β-Crystallins insolubilized by calpain II in vitro contain cleavage sites similar to β-crystallins insolubilized during cataract. FEBS Letters 324:3, pages 265-270.
Crossref

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