52
Views
80
CrossRef citations to date
0
Altmetric
Research Article

32P-Postlabeung Methods for DNA Adduct Detection: Overview and Critical Evaluation

&
Pages 67-85 | Published online: 22 Sep 2008

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (5)

H. Li, S. Cui, S. Wang, X. Jiang, S. Zhang, R. Zhang, P. P. Fu & X. Sun. (2015) Ultrasensitive UPLC–MS/MS method for analysis of etheno-DNA adducts in human white blood cells. Free Radical Research 49:9, pages 1049-1054.
Read now
V. Oreffo, R. Singh, K. J. Rich, D. E. G. Shuker, P. Carthew, R. Cordero, J. H. M. Van Delft & P. B. Farmer. (2000) DNA adducts in relation to lung tumour outcome are not markers of susceptibility following a single dose treatment of SWR, BALB/c and C57BL/6J mice with N-nitrosodiethylamine. Biomarkers 5:5, pages 323-340.
Read now
Guo-Dong Zhou, Natalie Supunpong Hernandez, Erika Randerath & Kurt Randerath. (1999) Acute Elevation by Short-Term Dietary Restriction or Food Deprivation of Type I I-Compound Levels in Rat Liver DNA. Nutrition and Cancer 35:1, pages 87-95.
Read now

Articles from other publishers (75)

Miao Han, Zaifeng Zhang, Si Liu, Youying Sheng, Michael Gatheru Waigi, Xiaojie Hu, Chao Qin & Wanting Ling. (2023) Genotoxicity of organic contaminants in the soil: A review based on bibliometric analysis and methodological progress. Chemosphere 313, pages 137318.
Crossref
Poguang Wang, Elisabeth Roider, Michael E. Coulter, Christopher A. Walsh, Caitlin S. Kramer, Penny J. Beuning & Roger W. Giese. (2021) DNA Adductomics by mass tag prelabeling. Rapid Communications in Mass Spectrometry 35:13.
Crossref
Kirsi Vähäkangas, Jarkko Loikkanen, Heidi Sahlman, Vesa Karttunen, Jenni Repo, Elina Sieppi, Maria Kummu, Pasi Huuskonen, Kirsi Myöhänen, Markus Storvik, Markku Pasanen, Päivi Myllynen & Olavi Pelkonen. 2019. Biomarkers in Toxicology. Biomarkers in Toxicology 303 339 .
A.P. Sanders, R.O. Wright, J.D. Groopman & J.-S. Wang. 2018. Comprehensive Toxicology. Comprehensive Toxicology 683 708 .
Alessia Stornetta, Maike Zimmermann, George D. Cimino, Paul T. Henderson & Shana J. Sturla. (2017) DNA Adducts from Anticancer Drugs as Candidate Predictive Markers for Precision Medicine. Chemical Research in Toxicology 30:1, pages 388-409.
Crossref
Miriam C. Poirier. (2016) Linking DNA adduct formation and human cancer risk in chemical carcinogenesis. Environmental and Molecular Mutagenesis 57:7, pages 499-507.
Crossref
Anton Bunschoten, Nynke S. van den Berg, Renato A. Valdés Olmos, Jacobus A. K. Blokland & Fijs W. B. van Leeuwen. 2016. Radioguided Surgery. Radioguided Surgery 75 101 .
Jinhwan Lim, Laura Ortiz, Brooke N. Nakamura, Yvonne D. Hoang, Jesus Banuelos, Victoria N. Flores, Jefferson Y. Chan & Ulrike Luderer. (2015) Effects of deletion of the transcription factor Nrf2 and benzo [a]pyrene treatment on ovarian follicles and ovarian surface epithelial cells in mice. Reproductive Toxicology 58, pages 24-32.
Crossref
M.J. Iatropoulos, J.-D. Duan, G. Schmuck & G.M. Williams. (2015) The urinary bladder carcinogen propoxur does not produce genotoxic effects in the urinary bladder of Wistar male rats. Experimental and Toxicologic Pathology 67:9, pages 453-458.
Crossref
Kirsi Vähäkangas, Jarkko Loikkanen, Heidi Sahlman, Vesa Karttunen, Jenni Repo, Elina Sieppi, Maria Kummu, Pasi Huuskonen, Kirsi Myöhänen, Markus Storvik, Markku Pasanen, Päivi Myllynen & Olavi Pelkonen. 2014. Biomarkers in Toxicology. Biomarkers in Toxicology 325 360 .
Manuel M. Paz & Chris A. Pritsos. 2012. Advances in Molecular Toxicology Volume 6. Advances in Molecular Toxicology Volume 6 243 299 .
Ignazio Garaguso, Roman Halter, Jacek Krzeminski, Shantu Amin & Jürgen Borlak. (2010) Method for the Rapid Detection and Molecular Characterization of DNA Alkylating Agents by MALDI-TOF Mass Spectrometry. Analytical Chemistry 82:20, pages 8573-8582.
Crossref
Véronique Thybaud. 2010. Cancer Risk Assessment. Cancer Risk Assessment 289 359 .
Shayne Cox GadAndreas Hartmann, Krista L. Dobo & Hans-J?rg Martus. 2010. Pharmaceutical Sciences Encyclopedia. Pharmaceutical Sciences Encyclopedia.
J.D. Groopman & J.-S. Wang. 2010. Comprehensive Toxicology. Comprehensive Toxicology 267 295 .
J.M. Links & J.D. Groopman. 2010. Comprehensive Toxicology. Comprehensive Toxicology 225 243 .
Thomas W. Kensler & John D. Groopman. (2009) Is It Time to Advance the Chemoprevention of Environmental Carcinogenesis with Microdosing Trials?. Cancer Prevention Research 2:12, pages 1003-1007.
Crossref
Marisa H. G. Medeiros. (2009) Exocyclic DNA Adducts as Biomarkers of Lipid Oxidation and Predictors of Disease. Challenges in Developing Sensitive and Specific Methods for Clinical Studies. Chemical Research in Toxicology 22:3, pages 419-425.
Crossref
Terry V. Zenser, Vijaya M. Lakshmi, Herman A.J. Schut, Hui-jia Zhou & P. David Josephy. (2009) Activation of aminoimidazole carcinogens by nitrosation: Mutagenicity and nucleotide adducts. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 673:2, pages 109-115.
Crossref
Jian-Dong Duan, Alan M. Jeffrey & Gary M. Williams. (2008) Assessment of the Medicines Lidocaine, Prilocaine, and Their Metabolites, 2,6-Dimethylaniline and 2-Methylaniline, for DNA Adduct Formation in Rat Tissues. Drug Metabolism and Disposition 36:8, pages 1470-1475.
Crossref
Koli Taghizadeh, Jose L McFaline, Bo Pang, Matthew Sullivan, Min Dong, Elaine Plummer & Peter C Dedon. (2008) Quantification of DNA damage products resulting from deamination, oxidation and reaction with products of lipid peroxidation by liquid chromatography isotope dilution tandem mass spectrometry. Nature Protocols 3:8, pages 1287-1298.
Crossref
Peter C DedonMichael S DeMottC Eric ElmquistErin G PrestwichJose L McFalineBo Pang. (2007) Challenges in developing DNA and RNA biomarkers of inflammation. Biomarkers in Medicine 1:2, pages 293-312.
Crossref
Franck A. Atienzar & Awadhesh N. Jha. (2006) The random amplified polymorphic DNA (RAPD) assay and related techniques applied to genotoxicity and carcinogenesis studies: A critical review. Mutation Research/Reviews in Mutation Research 613:2-3, pages 76-102.
Crossref
Volker M. Arlt. (2005) 3-Nitrobenzanthrone, a potential human cancer hazard in diesel exhaust and urban air pollution: a review of the evidence. Mutagenesis 20:6, pages 399-410.
Crossref
Bart Van den Driessche, Eddy L. Esmans, Annemie Van der Linden, Walter Van Dongen, Evi Schaerlaken, Filip Lemière, Erwin Witters & Zwi Berneman. (2005) First results of a quantitative study of DNA adducts of melphalan in the rat by isotope dilution mass spectrometry using capillary liquid chromatography coupled to electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry 19:14, pages 1999-2004.
Crossref
Jeffrey M. Karp, Kapila A. Rodrigo, Ping Pei, Matthew D. Pavlick, Jeffrey D. Andersen, Dennis J. McTigue, Henry W. Fields & Susan R. Mallery. (2005) Sanguinarine activates polycyclic aromatic hydrocarbon associated metabolic pathways in human oral keratinocytes and tissues. Toxicology Letters 158:1, pages 50-60.
Crossref
Amira Gamal Abdel-Rahman, Mohamed Farouk Allam, Maha M. El Gaafary, Mohamed Tarek Mansour & Mustafa Abdel-Aziz Mustafa. (2004) Assessment of carcinogenicity, using PAH-DNA adducts, in workers exposed to polycyclic aromatic hydrocarbonsEvaluación de la carcinogenidad en trabajadores expuestos a los hidrocarburos aromáticos policíclicos con aductos de ADN. Clinical and Translational Oncology 6:3, pages 159-164.
Crossref
Gary M. Williams, Michael J. Iatropoulos & Alan M. Jeffrey. (2016) Thresholds for the Effects of 2-Acetylaminofluorene in Rat Liver. Toxicologic Pathology 32:2_suppl, pages 85-91.
Crossref
Marie Stiborová, Martina Rupertová, Petr Hodek, Eva Frei & Heinz H. Schmeiser. (2004) Monitoring of DNA Adducts in Humans and 32P-Postlabelling Methods. A Review. Collection of Czechoslovak Chemical Communications 69:3, pages 476-498.
Crossref
Jin J Wang, William D Marshall, Brandon Law & Daniel M Lewis. (2003) Fragmentation patterns of DNA–benzo(a)pyrene diol epoxide adducts characterized by nanoflow LC/quadrupole time-of-flight mass spectrometry. International Journal of Mass Spectrometry 230:1, pages 45-55.
Crossref
Wen Luo, Rajan Gurjuar, Can Ozbal, Koli Taghizadeh, Arthur Lafleur, Ramachandra R. Dasari, Helmut Zarbl & William G. Thilly. (2002) Quantitative Detection of Benzo[ a ]pyrene Diolepoxide−DNA Adducts by Cryogenic Laser Induced Fluorescence . Chemical Research in Toxicology 16:1, pages 74-80.
Crossref
T.M.C.M. de Kok, H.J.J Moonen, J van Delft & F.J van Schooten. (2002) Methodologies for bulky DNA adduct analysis and biomonitoring of environmental and occupational exposures. Journal of Chromatography B 778:1-2, pages 345-355.
Crossref
John H Weisburger. (2002) Comments on the history and importance of aromatic and heterocyclic amines in public health. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 506-507, pages 9-20.
Crossref
Marie Stiborová, Vilı́m Šimánek, Eva Frei, Pavel Hobza & Jitka Ulrichová. (2002) DNA adduct formation from quaternary benzo[c]phenanthridine alkaloids sanguinarine and chelerythrine as revealed by the 32P-postlabeling technique. Chemico-Biological Interactions 140:3, pages 231-242.
Crossref
James A. Swenberg, Amy‐Joan L. Ham, Hasan Koc, David K. La, Eric J. Morinello, Brian F. Pachkowski, Asoka Ranasinghe & Patricia B. Upton. (2002) Methods for Measuring DNA Adducts and Abasic Sites II: Methods for Measurement of DNA Adducts . Current Protocols in Toxicology 12:1.
Crossref
Ninoslav Djelic & Dijana Djelic. (2002) Enhanced Sister-Chromatid Exchange Rate in Human Lymphocytes Exposed to 17? Estradiol in Vitro. Archives of Medical Research 33:2, pages 148-151.
Crossref
Marie Stiborová, Markéta Mikšanová, Vladimı́r Havlı́ček, Heinz H. Schmeiser & Eva Frei. (2002) Mechanism of peroxidase-mediated oxidation of carcinogenic o-anisidine and its binding to DNA. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 500:1-2, pages 49-66.
Crossref
Alan M. Jeffrey & M.I. Straub. 2002. Encyclopedia of Cancer. Encyclopedia of Cancer 345 357 .
M. Y. WANG & C. SU. (2001) Cancer Preventive Effect of Morinda citrifolia (Noni) . Annals of the New York Academy of Sciences 952:1, pages 161-168.
Crossref
Heather M McDiarmid, George R Douglas, Brenda L Coomber & P.David Josephy. (2001) Epithelial and fibroblast cell lines cultured from the transgenic BigBlue™ rat: an in vitro mutagenesis assay. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 497:1-2, pages 39-47.
Crossref
Volker M. Arlt, Christian A. Bieler, Walter Mier, Manfred Wiessler & Heinz H. Schmeiser. (2001) DNA adduct formation by the ubiquitous environmental contaminant 3-nitrobenzanthrone in rats determined by32P-postlabeling. International Journal of Cancer 93:3, pages 450-454.
Crossref
Woei G. Tan, Trevor J. Carnelley, Paula Murphy, Hailin Wang, Jane Lee, Sharon Barker, Michael Weinfeld & X.Chris Le. (2001) Detection of DNA adducts of benzo[a]pyrene using immunoelectrophoresis with laser-induced fluorescence. Journal of Chromatography A 924:1-2, pages 377-386.
Crossref
James Warren Gaubatz. 2001. The Role of DNA Damage and Repair in Cell Aging. The Role of DNA Damage and Repair in Cell Aging 41 72 .
M.Vijayaraj Reddy. (2000) Methods for Testing Compounds for DNA Adduct Formation. Regulatory Toxicology and Pharmacology 32:3, pages 256-263.
Crossref
Miriam C. Poirier, Regina M. Santella & Ainsley Weston. (2000) Carcinogen macromolecular adducts and their measurement. Carcinogenesis 21:3, pages 353-359.
Crossref
G Guetens, K Van Cauwenberghe, G De Boeck, R Maes, U.R Tjaden, J van der Greef, M Highley, A.T van Oosterom & E.A de Bruijn. (2000) Nanotechnology in bio/clinical analysis. Journal of Chromatography B: Biomedical Sciences and Applications 739:1, pages 139-150.
Crossref
S.J. Kok, R. Evertsen, N.H. Velthorst, U.A.Th. Brinkman & C. Gooijer. (2000) On the coupling of fluorescence line-narrowing spectroscopy and poly(ethylene)imine-cellulose thin-layer chromatography. Analytica Chimica Acta 405:1-2, pages 1-7.
Crossref
D. H. Phillips, P. B. Farmer, F. A. Beland, R. G. Nath, M. C. Poirier, M. V. Reddy & K. W. Turteltaub. (2000) Methods of DNA adduct determination and their application to testing compounds for genotoxicity. Environmental and Molecular Mutagenesis 35:3, pages 222-233.
Crossref
U. Brockstedt & W. Pfau. (1999) Reversed-phase high-performance liquid chromatographic separation of32P-labeled nucleotide adducts formed by food-derived carcinogenic aminoimidazoazarenes. Chromatographia 50:9-10, pages 547-552.
Crossref
M.Paul Chiarelli, HuaPing Wu, Alexandra M. Antunes & Paula S. Branco. (1999) Post-source decay production studies of aniline and methylaniline adducts of deoxyguanosine. Analytica Chimica Acta 397:1-3, pages 257-265.
Crossref
Filip Lemière, Koen Vanhoutte, Tim Jonckers, Radek Marek, Eddy L. Esmans, Magda Claeys, Elfriede Van den Eeckhout & Harry Van Onckelen. (1999) Differentiation between isomeric phenylglycidyl ether adducts of 2′-deoxyguanosine and 2′-deoxyguanosine-5′-monophosphate using liquid chromatography/electrospray tandem mass spectrometry. Journal of Mass Spectrometry 34:8, pages 820-834.
Crossref
J.S. Harvey, B.P. Lyons, T.S. Page, C. Stewart & J.M. Parry. (1999) An assessment of the genotoxic impact of the Sea Empress oil spill by the measurement of DNA adduct levels in selected invertebrate and vertebrate species. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 441:1, pages 103-114.
Crossref
Kurt Randerath, Erika Randerath, Guo-Dong Zhou & Donghui Li. (1999) Bulky endogenous DNA modifications (I-compounds)?possible structural origins and functional implications. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 424:1-2, pages 183-194.
Crossref
Christian A Bieler, Manfred Wiessler, Lothar Erdinger, Hitomi Suzuki, Takeji Enya & Heinz H Schmeiser. (1999) DNA adduct formation from the mutagenic air pollutant 3-nitrobenzanthrone. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 439:2, pages 307-311.
Crossref
K. Randerath, E. Randerath, G.D. Zhou, N. Supunpong, L.Y. He, T.J. McDonald & K.C. Donnelly. (1999) Genotoxicity of complex PAH mixtures recovered from contaminated lake sediments as assessed by three different methods. Environmental and Molecular Mutagenesis 33:4, pages 303-312.
Crossref
Peter Møller & Håkan Wallin. (1998) Adduct formation, mutagenesis and nucleotide excision repair of DNA damage produced by reactive oxygen species and lipid peroxidation product. Mutation Research/Reviews in Mutation Research 410:3, pages 271-290.
Crossref
Erik Kriek, Margarita Rojas, Kroum Alexandrov & Helmut Bartsch. (1998) Polycyclic aromatic hydrocarbon-DNA adducts in humans: relevance as biomarkers for exposure and cancer risk. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 400:1-2, pages 215-231.
Crossref
C.L Mitchelmore & J.K Chipman. (1998) DNA strand breakage in aquatic organisms and the potential value of the comet assay in environmental monitoring. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 399:2, pages 135-147.
Crossref
Kristine L Willett, Kurt Randerath, Guo-Dong Zhou & Stephen H Safe. (1998) Inhibition of CYP1A1-Dependent Activity by the Polynuclear Aromatic Hydrocarbon (PAH) Fluoranthene. Biochemical Pharmacology 55:6, pages 831-839.
Crossref
Wolfgang Pfau, Ulrike Brockstedt, Tomoyuki Shirai, Nobuyuki Ito & Hans Marquardt. (1997) Pancreatic DNA adducts formed in vitro and in vivo by the food mutagens 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-amino-3-methyl-9H-pyrido[2,3-b]indole (MeA?C). Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 378:1-2, pages 13-22.
Crossref
David H Phillips. (1997) Detection of DNA modifications by the 32P-postlabelling assay. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 378:1-2, pages 1-12.
Crossref
Sabine Hanelt, Rainer Helbig, Andreas Hartmann, Mathias Lang, Albrecht Seidel & Günter Speit. (1997) A comparative investigation of DNA adducts, DNA strand breaks and gene mutations induced by benzo[a]pyrene and (±)-anti-benzo[a]pyrene-7,8-diol 9,10-oxide in cultured human cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 390:1-2, pages 179-188.
Crossref
Gary M. Williams. (2016) Safety Assessment of Pharmaceuticals: Examples of Inadequate Assessments and a Mechanistic Approach to Assuring Adequate Assessment. Toxicologic Pathology 25:1, pages 32-38.
Crossref
Rajinder SinghGavain M. A. SweetmanPeter B. FarmerDavid E. G. ShukerKim J. Rich. (1997) Detection and Characterization of Two Major Ethylated Deoxyguanosine Adducts by High Performance Liquid Chromatography, Electrospray Mass Spectrometry, and 32 P-Postlabeling. Development of an Approach for Detection of Phosphotriesters . Chemical Research in Toxicology 10:1, pages 70-77.
Crossref
K. Randerath, G.-D. Zhou, E. Randerath, S.H. Safe & K.C. Donnelly. (1997) Comparative32P-postlabeling analysis of exogenous and endogenous DNA adducts in mouse skin exposed to a wood-preserving waste extract, a complex mixture of polycyclic and polychlorinated chemicals. Environmental and Molecular Mutagenesis 29:4, pages 372-378.
Crossref
Silvio De Flora, Alberto Izzotti, Kurt Randerath, Erika Randerath, Helmut Bartsch, Jagadeesan Nair, Roumen Balansky, Frederikjan van Schooten, Paolo Degan, Gilberto Fronza, Debra Walsh & Joellen Lewtas. (1996) DNA adducts and chronic degenerative diseases. Pathogenetic relevance and implications in preventive medicine. Mutation Research/Reviews in Genetic Toxicology 366:3, pages 197-238.
Crossref
N. Mabon, B. Moorthy, E. Randerath & K. Randerath. (1996) Monophosphate 32P-postlabeling assay of DNA adducts from 1,2:3,4-diepoxybutane, the most genotoxic metabolite of 1,3-butadiene: in vitro methodological studies and in vivo dosimetry. Mutation Research/Genetic Toxicology 371:1-2, pages 87-104.
Crossref
Rosalie K. Elespuru. (1996) Future approaches to genetic toxicology risk assessment. Mutation Research/Reviews in Genetic Toxicology 365:1-3, pages 191-204.
Crossref
David K. La & James A. Swenberg. (1996) DNA adducts: biological markers of exposure and potential applications to risk assessment. Mutation Research/Reviews in Genetic Toxicology 365:1-3, pages 129-146.
Crossref
Rasoul S. Kerdar. (1996) 32P-postlabeling analysis of bulky aromatic and non-aromatic adducts. Experimental and Toxicologic Pathology 48:5, pages 461-466.
Crossref
Andreas Baumann, Rasoul S. Kerdar, Peter Cramer, Werner Feser, Hartmut Blode, Andreas Salomon & Wilhelm Kuhnz. (2009) Use of Rat and Human Liver Slices for the Detection of Steroid Hormone‐Induced DNA‐Adducts in vitro by Means of the 32 P‐Postlabeling Technique . Pharmacology & Toxicology 78:4, pages 214-223.
Crossref
Gary M. Williams, Michael J. Iatropoulos & John H. Weisburger. (1996) Chemical carcinogen mechanisms of action and implications for testing methodology. Experimental and Toxicologic Pathology 48:2-3, pages 101-111.
Crossref
Shin Han, Constance M. Harris, Thomas M. Harris, Hye-Young Hong Kim & Seong J. Kim. (1996) Synthesis of Deoxyadenosine 3‘-Phosphates Bearing Cis and Trans Adducts of 7β,8α-Dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo[ a ]pyrene:  Standards for 32 P-Postlabeling Assays . The Journal of Organic Chemistry 61:1, pages 174-178.
Crossref
John P. Barry, Curt Norwood & Paul Vouros. (1996) Detection and Identification of Benzo[ a ]pyrene Diol Epoxide Adducts to DNA Utilizing Capillary Electrophoresis-Electrospray Mass Spectrometry . Analytical Chemistry 68:8, pages 1432-1438.
Crossref
Gérard Keith & Guy Dirheimer. (1995) Postlabeling: a sensitive method for studying DNA adducts and their role in carcinogenesis. Current Opinion in Biotechnology 6:1, pages 3-11.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.