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Hemoglobin
international journal for hemoglobin research
Volume 1, 1977 - Issue 6
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Original Article

An Improved Chromatographic Procedure for Quantitation of Human Fetal Hemoglobin

E. C. Abraham Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA
,
A. Reese Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA
,
M. Stallings Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA
,
F. A. Carver Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA
&
T. H. J. Huisman Laboratory of Protein Chemistry, Department of Cell and Molecular Biology and Comprehensive Sickle Cell Center, Medical College of Georgia, and Veterans Administration Hospital, Augusta, Georgia, 30901, USA
Pages 547-560 | Received 29 Apr 1977, Accepted 15 May 1977, Published online: 07 Jul 2009

Citations (20)

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Svetlana Pulis, Christian A. Scerri, Pierre Schembri Wismayer, Ruth Galdies, Stephanie Bezzina Wettinger & Alex E. Felice. (2007) Developmental Effect of the XmnI Site on Gγ-Globin Gene Expression Among Newborn Hb F-Malta-I [Gγ117(G19)His→Arg, CAT→CGT] Heterozygotes and Adult β+-Thalassemia Homozygotes. Hemoglobin 31:1, pages 71-82.
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Georgi D. Efremov. (2001) FORTY-FOUR YEARS (1955–1999) DEVOTED TO HEMOGLOBIN RESEARCH: TITUS H. J. HUISMAN (1923–1999). Hemoglobin 25:2, pages 125-168.
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William L. Marsh & Harold M. Koenig. (1982) The Laboratory Evaluation of Microcytic Red Blood Cells. CRC Critical Reviews in Clinical Laboratory Sciences 16:3, pages 195-254.
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G. D. Efremov, B. Markovska, N. Stojanovski, G. Petkov, N. Nikolov & T. H. J. Huisman. (1981) The Use of Globin Chain Electrophoresis in Polyacrylamide Gels for the Quantitation of the Gγ to Aγ Ratio in fetal Hemoglobih. Hemoglobin 5:7-8, pages 637-651.
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T. H. J. Huisman & B. Webber. (1981) The Frequency of the AγT Gene in the Presence and Absence of the βs or βC Gene in the Black Poplilation of the Southeastern USA. Hemoglobin 5:5, pages 441-451.
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T. H. J. Huisman. (1980) Chromatographic Methods for the Separation of Normal and Abnormal Hemoglobins. Hemoglobin 4:3-4, pages 527-539.
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K. Shimizu, J. B. Wilson & T. H. J. Huisman. (1980) The Determination of the Percentages of Gγ and Aγ Chains in Human Fetal Hemoglobin by HPLC. Hemoglobin 4:3-4, pages 487-496.
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E. C. Abraham, J. Carver, J. Döbler, P. F. Milner & T. H. J. Huisman. (1979) Microchromatographic Quantitation of Fetal Hemoglobin in Patients with Sickle Cell Disease. Hemoglobin 3:5, pages 341-351.
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T. H. J. Huisman, G. D. Efremov, A. L. Reese, J. S. Howard, M. E. Gravely, H. F. Harris & J. B. Wilson. (1979) The Synthesis of Fetal Hemoglobin Types in red Blood Cells and in BFU-E Derived Colonies from Peripheral Blood of Patients with Sickle Cell Anemia, β+- and δβ-Thalassemia, Various forms of Hereditary Persistence of Fetal Hemoglobin, Normal Adults and Newborn. Hemoglobin 3:4, pages 223-252.
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Articles from other publishers (11)

Ariel Weissman & Zion Hagay. (2009) Glycosylated haemoglobin in the fetus: Chemistry, laboratory measurements and future clinical implications. Diabetes/Metabolism Reviews 10:1, pages 45-62.
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Y. Enoki, Y. Ohga, S. Sakata & H. Kohzuki. (2008) Postnatal transition of γ‐globin gene expression in normal Japanese population. British Journal of Haematology 79:4, pages 628-633.
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Yasunori Enoki, Yoshimi Ohga, Rie Nagano & Kazuhito Matsumura. (1990) Efficient method of haemoglobin F enrichment in adult haemolysate for determination of the γ-chain isoforms by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications 533, pages 63-71.
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I. Bakioglu, A.L. Reese & T.H.J. Huisman. (1984) A comparison of two procedures useful for the isolation of Hb F from adult red blood cells and for the quantitation of the types of μ chain by high-performance liquid chromatography. Journal of Chromatography A 295, pages 171-177.
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P. Basset, F. Braconnier & J. Rosa. (1982) An update on electrophoretic and chromatographic methods in the diagnosis of hemoglobinopathies. Journal of Chromatography B: Biomedical Sciences and Applications 227:2, pages 267-304.
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Hossam E. Fadel, A. Reynolds, M. Stallings & E.C. Abraham. (1981) Minor (glycosylated) hemoglobins in cord blood of infants of normal and diabetic mothers. American Journal of Obstetrics and Gynecology 139:4, pages 397-402.
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A. Francina, E. Dorleac & H. Cloppet. (1981) Carrier-ampholyte displacement chromatography (chromatofocusing) on ion-exchange papers. Journal of Chromatography B: Biomedical Sciences and Applications 222:1, pages 116-119.
Crossref
T. H. J. Huisman, A. L. Reese, M. E. Gravely, H. Harris & J. B. Wilson. (2006) The synthesis of the G γ and A γ chains of human fetal hemoglobin in erythroid colonies cultured from peripheral blood BFUe's of normal adults and newborn and of subjects with an A γ or a G γ chain abnormal fetal hemoglobin . American Journal of Hematology 9:2, pages 137-150.
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E. C. Abraham & T. H. J. Huisman. (2006) Functional characteristics of red cells of sickle cell anemia patients with and without α‐thalassemia‐2. American Journal of Hematology 8:2, pages 133-138.
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R.F.L. James, J. Shuster, S.O. Freedman & P. Gold. (1980) The isolation of the γ subunit of fetal hemoglobin (HbF) and its use in a radioimmunoassay for HbF. Journal of Immunological Methods 32:3, pages 251-260.
Crossref
W.A. Schroeder, Laurence A. Pace & T.H.J. Huisman. (1978) Microchromatography of hemoglobins. Journal of Chromatography B: Biomedical Sciences and Applications 145:2, pages 203-212.
Crossref

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