Publication Cover
Hemoglobin
international journal for hemoglobin research
Volume 3, 1979 - Issue 5
17
Views
36
CrossRef citations to date
0
Altmetric
Original Article

Preliminary Experiments in the Separation of Globin Chains by high Performance Liquid Chromatography

, &
Pages 353-358 | Received 16 Jul 1979, Accepted 09 Aug 1979, Published online: 07 Jul 2009

Keep up to date with the latest research on this topic with citation updates for this article.

Read on this site (13)

S. Rahbar & Y. Asmerom. (1989) Rapid HPLC Techniques for Globin Chain Synthesis Studies. Hemoglobin 13:5, pages 475-487.
Read now
MarkkuT. Parviainen, Toivo Halonen, Tapani Ahola, Aimo Harmoinen, Pauli Vuorinen & Ilkka Mononen. (1987) Hydrophobic Interaction Chromatography in the Detection of Hemoglobinopathies. Journal of Liquid Chromatography 10:13, pages 2951-2963.
Read now
S. M. Hanash & D. N. Shapiro. (1981) Separation of Human Hemoglobins by ION Exchange High Performance Liquid Chromatography. Hemoglobin 5:2, pages 165-175.
Read now
G. Dincol, C. Altay, M. Aksoy, A. Gurgey, A. E. Felice & T. H. J. Huisman. (1981) Clinical and Hematological Evaluation of two δ0 δ0 - Thalassemia Homozygotes. Hemoglobin 5:2, pages 153-164.
Read now
G. D. Efremov, B. Markovska, N. Stojanovski, G. Petkov, N. Nikolov & T. H. J. Huisman. (1981) The Use of Globin Chain Electrophoresis in Polyacrylamide Gels for the Quantitation of the Gγ to Aγ Ratio in fetal Hemoglobih. Hemoglobin 5:7-8, pages 637-651.
Read now
JoanB. Shelton, J. Roger Shelton & W.A. Schroeder. (1981) Further Experiments in the Separation of Globin Chains by High Performance Liquid Chromatography. Journal of Liquid Chromatography 4:8, pages 1381-1392.
Read now
BlancheP. Alter, SabraC. Goff, ThomasJ. Klonowski & MichaelD. Garrick. (1981) Chemical Evidence for the Separation of Gy and AY Globin Chains by Polyacrylamide Gel Electrophoresis in Urea and Triton X-100. Preparative Biochemistry 11:2, pages 217-228.
Read now
W. A. Schroeder, Joan B. Shelton & J. Roger Shelton. (1980) Separation of Hemoglobin Peptides by high Performance Liquid Chromatography (HPLC). Hemoglobin 4:3-4, pages 551-559.
Read now
K. Shimizu, J. B. Wilson & T. H. J. Huisman. (1980) The Determination of the Percentages of Gγ and Aγ Chains in Human Fetal Hemoglobin by HPLC. Hemoglobin 4:3-4, pages 487-496.
Read now
L. F. Bernini, P. C. Giordano & D. A. F. van Lith. (1980) Frequency and Quantitative Levels of Tγ Chain (Hb F Sardinia) in the Fetal Hemoglobin of Newborn and Fetuses. Hemoglobin 4:3-4, pages 469-482.
Read now
BlancheP. Alter, SabraC. Goff, ThomasJ. Klonowski & MichaelD. Garrick. (1980) Chemical Evidence for the Separation of Gγ and Aγ Globin Chains by Polyacrylamide Gel Electrophoresis in Urea and Triton X-100. Preparative Biochemistry 10:5, pages 633-644.
Read now

Articles from other publishers (23)

Hooshang Nemati, Gholamreza Bahrami & Zohreh Rahimi. (2010) Rapid separation of human globin chains in normal and thalassemia patients by RP-HPLC. Molecular Biology Reports 38:5, pages 3213-3218.
Crossref
Y. Enoki, Y. Ohga, S. Sakata & H. Kohzuki. (2008) Postnatal transition of γ‐globin gene expression in normal Japanese population. British Journal of Haematology 79:4, pages 628-633.
Crossref
Yasunori Enoki, Yoshimi Ohga, Rie Nagano & Kazuhito Matsumura. (1990) Efficient method of haemoglobin F enrichment in adult haemolysate for determination of the γ-chain isoforms by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications 533, pages 63-71.
Crossref
Eleanor Canova-Davis*Ida P. Baldonado & Glen M. Teshima. (1990) Characterization of chemically synthesized human relaxin by high-performance liquid chromatography. Journal of Chromatography A 508, pages 81-96.
Crossref
Yoshinao Wada, Takekiyo Matsuo & Toru Sakurai. (2005) Structure elucidation of hemoglobin variants and other proteins by digit‐printing method. Mass Spectrometry Reviews 8:5, pages 379-434.
Crossref
V.A. Spivak & I.N. Lutsenko. (1988) Micropreparative electrophoresis of globin chains on cellulose acetate film in structural identification of abnormal human hemoglobins. Analytical Biochemistry 171:1, pages 108-112.
Crossref
. 1988. High-Performance Liquid Chromatography of Biopolymers and Biooligomers - Separation of Individual Compound Classes. High-Performance Liquid Chromatography of Biopolymers and Biooligomers - Separation of Individual Compound Classes B1 B62 .
T.H.J. Huisman. (1987) Separation of hemoglobins and hemoglobin chains by high-performance liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications 418, pages 277-304.
Crossref
Gianfranco Cossu, Mario Manca, Pier Giorgio Righetti, Elisabetta Gianazza, VÉRonique Baudin, Henri Wajcman & Adriana Bianchi‐Bosisio. (2005) Detection of neutral amino acid mutations by immobilized pH gradients: The case of the Tγ variant in fetal hemoglobin Sardinia. ELECTROPHORESIS 7:5, pages 213-216.
Crossref
Leonello Leone, Michele Monteleone, Vilma Gabutti & Cristina Amione. (1985) Reversed-phase high-performance liquid chromatography of human haemoglobin chains. Journal of Chromatography A 321, pages 407-419.
Crossref
T.H.J. HUISMAN & J.B. WILSON. 1985. 1029 1036 .
Jan-Olof Jeppsson, Ivan Källman, Göran Lindgren & Lars G. Fägerstam. (1984) Hb-linköping (β36 Pro → Thr): a new hemoglobin mutant characterized by reversed-phase high-performance liquid chromatography. Journal of Chromatography A 297, pages 31-36.
Crossref
Z I Randhawa, R T Jones & L E Lie-Injo. (1984) Human hemoglobin Portland II (zeta 2 beta 2). Isolation and characterization of Portland hemoglobin components and their constituent globin chains.. Journal of Biological Chemistry 259:11, pages 7325-7330.
Crossref
V. Baudin & H. Wajcman. (1984) Rapid high-performance liquid chromatographic method for the separation of the three types of γ-chain of human fetal haemoglobin. Journal of Chromatography A 299, pages 495-497.
Crossref
H. Engelhardt & H. Müller. (1984) Optimal conditions for the reversed-phase chromatography of proteins. Chromatographia 19:1, pages 77-84.
Crossref
Zafar I. Randhawa, Richard T. Jones & Luan E. Lie-Injo. (1983) Separation of zeta (ζ) and various gamma (γ) chains of human embryonic hemoglobin Portland I by reverse-phase high-performance liquid chromatography. Analytical Biochemistry 129:1, pages 184-191.
Crossref
L.F. Congote & A.G. Kendall. (1982) Rapid analysis of labeled globin chains without acetone precipitation or dialysis by high-pressure liquid chromatography and ion-exchange chromatography. Analytical Biochemistry 123:1, pages 124-132.
Crossref
Y. Kroviarski, S. Cochet, P. Boivin & O. Bertrand. (1982) Rapid separation of human globin chains by high flow-rate chromatography under low pressure using CM-Trisacryl M. Journal of Chromatography B: Biomedical Sciences and Applications 228, pages 298-304.
Crossref
P. Basset, F. Braconnier & J. Rosa. (1982) An update on electrophoretic and chromatographic methods in the diagnosis of hemoglobinopathies. Journal of Chromatography B: Biomedical Sciences and Applications 227:2, pages 267-304.
Crossref
W. A. Schroeder, Joan B. Shelton, J. Roger Shelton, Darleen Powars, Shlomo Friedman, James Baker, Jerry Z. Finklestein, B. Miller, Cage S. Johnson, J. R. Sharpsteen, Lance Sieger & Eric Kawaoka. (1982) Identification of eleven human hemoglobin variants by high-performance liquid chromatography: Additional data on functional properties and clinical expression. Biochemical Genetics 20:1-2, pages 133-152.
Crossref
J. E. Fuhr, E. Bamberger, C. B. Lozzio, B. B. Lozzio, A. E. Felice, G. Altay, B. B. Webber, A. L. Reese, S. M. Mayson & T. H. J. Huisman. (2006) Identification and quantitation of embryonic and three types of fetal hemoglobin produced on induction of the human pluripotent leukemia cell line K‐562 with hemin. American Journal of Hematology 12:1, pages 1-12.
Crossref
Jean B. Henson, Jacqueline R. Carver, J.B. Wilson & T.H.J. Huisman. (1980) Carboxylmethyl-cellulose microchromatography for the quantitation of hemoglobin Bart's (γ4) and its use in the detection of the α-thalassemia conditions. Journal of Chromatography A 198:4, pages 443-448.
Crossref
T. H. J. Huisman & J. B. Wilson. (2006) Recent advances in the quantitation of human fetal hemoglobins with different gamma chains. American Journal of Hematology 9:2, pages 225-235.
Crossref

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.