Advanced search
Publication Cover
Autoimmunity Volume 14, 1993 - Issue 1
Submit an article Journal homepage
27
Views
66
CrossRef citations to date
0
Altmetric
Original Article

Autoantibodies to the Ro/Ssa Antigen are Conformation Dependent. I: Anti-60 kD Antibodies are Mainly Directed to the Native Protein; Anti-52 kD Antibodies are Mainly Directed to the Denatured Protein

Yasuhiko Itoh Arthritis and Immunology Program. Oklahoma Medical Research Foundation, Department of Medicine, College of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, 73104
&
Morris Reichlin Arthritis and Immunology Program. Oklahoma Medical Research Foundation, Department of Medicine, College of Medicine, Oklahoma University Health Sciences Center, Oklahoma City, Oklahoma, 73104
Pages 57-65 | Received 03 Jun 1992, Accepted 17 Jul 1992, Published online: 07 Jul 2009

Citations (66)

Keep up to date with the latest research on this topic with citation updates for this article.

Subscribe to citation updates

Read on this site (5)

C. Thompson, D. A. Pomeranz Krummel, H. Jacobsen, K. NagaI & A. Cooke. (2006) Identification of nuclear spliceosomal antigens targeted by NOD mouse antibodies following sodium iodide intake. Autoimmunity 39:2, pages 99-106.
Read now
F. Franceschini & I. Cavazzana. (2005) Anti-Ro/SSA and La/SSB antibodies. Autoimmunity 38:1, pages 55-63.
Read now
J.P. Buyon. (1998) Neonatal lupus and autoantibodies reactive with SSA/Ro-SSB/La (abstract). Scandinavian Journal of Rheumatology 27:sup107, pages 23-30.
Read now
J. P. Buyon. (1996) Neonatal Lupus: Bedside to Bench and Back. Scandinavian Journal of Rheumatology 25:5, pages 271-276.
Read now
Tohru Igarashi, Yasuhiko Itoh, Yoshitaka Fukunaga & Masao Yamamoto. (1995) Stress-Induced Cell Surface Expression and Antigenic Alteration of the Ro/SSA Autoantigen. Autoimmunity 22:1, pages 33-42.
Read now

Articles from other publishers (61)

Viktoryia Laurynenka, Lili Ding, Kenneth M. Kaufman, Judith A. James & John B. Harley. (2022) A High Prevalence of Anti-EBNA1 Heteroantibodies in Systemic Lupus Erythematosus (SLE) Supports Anti-EBNA1 as an Origin for SLE Autoantibodies. Frontiers in Immunology 13.
Crossref
Geoffrey Urbanski, Aline Gury, Pascale Jeannin, Alain Chevailler, Pierre Lozac’h, Pascal Reynier, Christian Lavigne, Carole Lacout & Emeline Vinatier. (2022) Discordant Predictions of Extraglandular Involvement in Primary Sjögren’s Syndrome According to the Anti-SSA/Ro60 Antibodies Detection Assay in a Cohort Study. Journal of Clinical Medicine 11:1, pages 242.
Crossref
Sandra G. Williams & Sandra L. Wolin. (2021) The Autoantigen Repertoire and the Microbial RNP World. Trends in Molecular Medicine 27:5, pages 422-435.
Crossref
Thomas Bahmer, Micaela Romagnoli, Francesco Girelli, Martin Claussen & Klaus F. Rabe. (2016) The use of auto-antibody testing in the evaluation of interstitial lung disease (ILD) – A practical approach for the pulmonologist. Respiratory Medicine 113, pages 80-92.
Crossref
Kazuhiro Ikeda & Satoshi Inoue. 2012. TRIM/RBCC Proteins. TRIM/RBCC Proteins 27 37 .
Jill P. Buyon & Deborah M. Friedman. 2011. Systemic Lupus Erythematosus. Systemic Lupus Erythematosus 541 571 .
G BOIRE, M GENDRON, N MONAST, B BASTIN & H A MÉNARD. (1995) Purification of antigenically intact Ro ribonucleoproteins; biochemical and Immunological evidence that the 52-kD protein is not a Ro protein. Clinical and Experimental Immunology 100:3, pages 489-498.
Crossref
M Reichlin. (1995) Autoantibodies to the RoRNP particles. Clinical and Experimental Immunology 99:1, pages 7-9.
Crossref
C H A VELDHOVEN, G J M PRUIJN, J F MEILOF, J P H THIJSSEN, A W C M VAN DER KEMP, W J VAN VENROOIJ & R J T SMEENK. (1995) Characterization of murine monoclonal antibodies against 60-kD Ro/SS-A and La/SS-B autoantigens. Clinical and Experimental Immunology 101:1, pages 45-54.
Crossref
M B FRANK, K ITOH & V McCUBBIN. (1994) Epitope mapping of the 52-kD Ro/SSA autoantigen. Clinical and Experimental Immunology 95:3, pages 390-396.
Crossref
A G Terzoglou, J G Routsias, H M Moutsopoulos & A G Tzioufas. (2006) Post-translational modifications of the major linear epitope 169–190aa of Ro60 kDa autoantigen alter the autoantibody binding. Clinical and Experimental Immunology 146:1, pages 60-65.
Crossref
Sandra L. Wolin & Karin M. Reinisch. (2006) The Ro 60 kDa autoantigen comes into focus: Interpreting epitope mapping experiments on the basis of structure. Autoimmunity Reviews 5:6, pages 367-372.
Crossref
P. Youinou & Y. Renaudineau. (2006) Les nouveaux autoanticorps du syndrome de Gougerot-Sjögren primaire. Immuno-analyse & Biologie Spécialisée 21:3, pages 158-164.
Crossref
Keiji Wada & Tetsu Kamitani. (2006) Autoantigen Ro52 is an E3 ubiquitin ligase. Biochemical and Biophysical Research Communications 339:1, pages 415-421.
Crossref
Kazuhiro Ikeda, Satoshi Inoue & Masami Muramatsu. 2005. Zinc Finger Proteins. Zinc Finger Proteins 106 113 .
Jill P. Buyon. 2004. Systemic Lupus Erythematosus. Systemic Lupus Erythematosus 449 484 .
Jill P. Buyon & Robert M. Clancy. (2003) Maternal autoantibodies and congenital heart block: mediators, markers, and therapeutic approach. Seminars in Arthritis and Rheumatism 33:3, pages 140-154.
Crossref
Joanna M. Burch, Ronald J. Sokol, Michael R. Narkewicz, Morris Reichlin, Mark Barton Frank, Todd MacKenzie & Lela A. Lee. (2003) Autoantibodies in Mothers of Children with Neonatal Liver Disease. Journal of Pediatric Gastroenterology and Nutrition 37:3, pages 262-267.
Crossref
Antonio Brucato & Jill P Buyon. 2003. The Hearth in Systemic Autoimmune Diseases. The Hearth in Systemic Autoimmune Diseases 163 188 .
Taeko Fukuda-Kamitani & Tetsu Kamitani. (2002) Ubiquitination of Ro52 autoantigen. Biochemical and Biophysical Research Communications 295:4, pages 774-778.
Crossref
Tawatchai Paisansinsup, Umesh S. Deshmukh, Vaidehi R. Chowdhary, Harvinder S. Luthra, Shu Man Fu & Chella S. David. (2002) HLA Class II Influences the Immune Response and Antibody Diversification to Ro60/Sjögren’s Syndrome-A: Heightened Antibody Responses and Epitope Spreading in Mice Expressing HLA-DR molecules. The Journal of Immunology 168:11, pages 5876-5884.
Crossref
B T Kurien, J Newland, C Paczkowski, K L Moore & R H Scofield. (2000) Association of neutropenia in systemic lupus erythematosus (SLE) with anti-Ro and binding of an immunologically cross-reactive neutrophil membrane antigen. Clinical and Experimental Immunology 120:1, pages 209-217.
Crossref
A-K HALSE, M WAHREN-HERLENIUS & R JONSSON. (1999) Ro/SS-A- and La/SS-B-reactive B lymphocytes in peripheral blood of patients with Sjögren's syndrome. Clinical and Experimental Immunology 115:1, pages 208-213.
Crossref
J. Wenzel, R. Gerdsen, M. Uerlich, R. Bauer, T. Bieber & I. Boehm. (2001) Antibodies targeting extractable nuclear antigens: historical development and current knowledge. British Journal of Dermatology 145:6, pages 859-867.
Crossref
A N ScofieldB T Kurien, T P Gordon & R H Scofield. (2016) Can B cell epitopes of 60 kDa Ro distinguish systemic lupus erythematosus from Sjögren's syndrome?. Lupus 10:8, pages 547-553.
Crossref
Jill P. Buyon, Mimi Y. Kim, Joshua A. Copel & Deborah M. Friedman. (2001) Anti-Ro/SSA antibodies and congenital heart block: Necessary but not sufficient. Arthritis & Rheumatism 44:8, pages 1723-1727.
Crossref
B. T. Kurien, T. L. Chambers, P. Y. Thomas, M. B. Frank & R. H. Scofield. (2001) Autoantibody to the Leucine Zipper Region of 52 kDa Ro/SSA Binds Native 60 kDa Ro/SSA: Identification of a Tertiary Epitope with Components from 60 kDa Ro/SSA and 52 kDa Ro/SSA. Scandinavian Journal of Immunology 53:3, pages 268-276.
Crossref
John B. Harley, R. Hal Scofield & Judith A. James. 2000. Molecular Mimicry, Microbes, and Autoimmunity. Molecular Mimicry, Microbes, and Autoimmunity 109 126 .
Blomberg, Ronnblom, Wallgren, Nilsson & Karlsson‐Parra. (2001) Anti‐SSA/Ro Antibody Determination by Enzyme‐Linked Immunosorbent Assay as a Supplement to Standard Immunofluorescence in Antinuclear Antibody Screening. Scandinavian Journal of Immunology 51:6, pages 612-617.
Crossref
N. M. Moutsopoulos, J. G. Routsias, P. G. Vlachoyiannopoulos, A. G. Tzioufas & H. M. Moutsopoulos. (2000) B-Cell Epitopes of Intracellular Autoantigens: Myth and Reality. Molecular Medicine 6:3, pages 141-151.
Crossref
W. Pollock & B. H. Toh. (1999) Routine immunofluorescence detection of Ro/SS-A autoantibody using HEp- 2 cells transfected with human 60 kDa Ro/SS-A. Journal of Clinical Pathology 52:9, pages 684-687.
Crossref
Martín Yago, Miguel A Belmonte, Maria J Olmos, Juan Beltran, Carlos Teruel & Marian Segarra. (2016) Detecting Anti-SSA and Anti-SSB Antibodies in Routine Analysis: A Comparison between Double Immunodiffusion and Immunoblotting. Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 36:3, pages 365-371.
Crossref
R. Hal Scofield, A. Darise Farris, Angela C. Horsfall & John B. Harley. (1999) Fine specificity of the autoimmune response to the Ro/SSA and La/SSB ribonucleoproteins. Arthritis & Rheumatism 42:2, pages 199-209.
Crossref
Eldad Ben-Chetrit. 1999. The Decade of Autoimmunity. The Decade of Autoimmunity 143 148 .
P. J. Maddison. 1999. Rheumaderm. Rheumaderm 141 145 .
Sachiko Miyagawa, Toshihiko Shirai, Kumiko Yanagi, Akira Yoshioka, Kin-ichi Kidoguchi & Yoshio Hayashi. (1998) Neonatal Lupus Erythematosus: Maternal IgG Antibodies Bind to a Recombinant NH2-Terminal Fusion Protein Encoded by Human α-Fodrin cDNA. Journal of Investigative Dermatology 111:6, pages 1189-1192.
Crossref
Sachiko Miyagawa, Koji Shinohara, Mitsuru Nakajima, Kin-Ichi Kidoguchi, Tomio Fujita, Takaya Fukumoto, Akira Yoshioka, Kazuhiro Dohi & Toshihiko Shirai. (1998) Polymorphisms of HLA class II genes and autoimmune responses to Ro/SS-A-La/SS-B among Japanese subjects. Arthritis & Rheumatism 41:5, pages 927-934.
Crossref
Jingsong Wang, Xingwen Dong, Lovorka Stojanov, Donald Kimpel, Minoru Satoh & Westley H. Reeves. (1997) Human autoantibodies stabilize the quaternary structure of Ku antigen. Arthritis & Rheumatism 40:7, pages 1344-1353.
Crossref
Jingsong Wang, Xingwen Dong, Lovorka Stojanov, Donald Kimpel, Minoru Satoh & Westley H. Reeves. (1997) Human autoantibodies stabilize the quaternary structure of Ku antigen. Arthritis & Rheumatism 40:7, pages 1344-1353.
Crossref
Chung‐E Tseng, Edward K. L. Chan, Eugenia Miranda, Michael Gross, Francis Di Donato & Jill P. Buyon. (2005) The 52‐kd protein as a target of intermolecular spreading of the immune response to components of the SS‐A/Ro‐SS‐B/La complex. Arthritis & Rheumatism 40:5, pages 936-944.
Crossref
Jill P. Buyon, Chung‐E Tseng, Francis Di Donato, William Rashbaum, Allan Morris & Edward K. L. Chan. (2005) Cardiac expression of 52β, an alternative transcript of the congenital heart block‐associated 52‐kd SS‐A/Ro autoantigen, is maximal during fetal development. Arthritis & Rheumatism 40:4, pages 655-660.
Crossref
Chung-E. Tseng & Jill P. Buyon. (1997) NEONATAL LUPUS SYNDROMES. Rheumatic Disease Clinics of North America 23:1, pages 31-54.
Crossref
TS Lieu & RD Sontheimer. (2016) A subpopulation of WIL-2 cell calreticulin molecules is associated with RO/SS-A ribonucleoprotein particles. Lupus 6:1, pages 40-47.
Crossref
Concepción González, Teresa Martin, Tomás Arroyo, María García-Isidoro, José A. Navajo & José M. González-Buitrago. (1997) Comparison and variation of different methodologies for the detection of autoantibodies to nuclear antigens (ANA). Journal of Clinical Laboratory Analysis 11:6, pages 388-392.
Crossref
Westley H. Reeves & Minoru Satoh. (1996) Features of autoantigens. Molecular Biology Reports 23:3-4, pages 217-226.
Crossref
Lela A. Lee, Kathleen Alvarez, Timothy Gross & John B. Harley. (1996) The Recognition of Human 60-kDa Ro Ribonucleoprotein Particles by Antibodies Associated With Cutaneous Lupus and Neonatal Lupus. Journal of Investigative Dermatology 107:2, pages 225-228.
Crossref
Thomas T Provost, Rosemarie Watson & Eva Simmons-O'Brien. (1996) Significance of the anti-Ro(SS-A) antibody in evaluation of patients with cutaneous manifestations of a connective tissue disease. Journal of the American Academy of Dermatology 35:2, pages 147-169.
Crossref
Morris Reichlin & R. Hal Scofield. 1996. Autoantibodies. Autoantibodies 783 788 .
Marek MichalakRichard D. Sontheimer, Tho Q. Nguyen, Shih-Tsung Cheng, Tsu-San Lieu & J. Donald Capra. 1996. Calreticulin. Calreticulin 117 139 .
Tomohiro Kato, Hiroko Sasakawa, Satoshi Suzuki, Manami Shirako, Fumio Tashiro, Kusuki Nishioka & Kazuhiko Yamamoto. (2005) Autoepitopes of the 52‐kd ss‐a/ro molecule. Arthritis & Rheumatism 38:7, pages 990-998.
Crossref
Jane C. Atkinson, Leah S. Royce, Robert Wellner, Stanley R. Pillemer, Deborah Bermudez & Philip C. Fox. (2006) Anti‐salivary antibodies in primary Sjögren's syndrome. Journal of Oral Pathology & Medicine 24:5, pages 206-212.
Crossref
Eva Simmons-OʼBrien, Suephy Chen, Rosemarie Watson, Christian Antoni, Michelle Petri, Marc Hochberg, Mary Betty Stevens & Thomas T. Provost. (1995) One Hundred Anti-Ro (SS-A) Antibody Positive Patients: A 10-Year Follow-Up. Medicine 74:3, pages 109-130.
Crossref
A.P. SHETH, N.B. ESTERLY, S.L. RATOOSH, J.P. SMITH, A.A. HEBERT & E. SILVERMAN. (2006) U1RNP positive neonatal lupus erythematosus: association with anti-La antibodies?. British Journal of Dermatology 132:4, pages 520-526.
Crossref
S. C. HUANG, H. YU, R. H. SCOFIELD & J. B. HARLEY. (1995) Human Anti-Ro Autoantibodies Bind Peptides Accessible to the Surface of the Native Ro Autoantigen. Scandinavian Journal of Immunology 41:3, pages 220-228.
Crossref
Minoru Satoh, Edqard L Treadwell & Westley H Reeves. (1995) Pristane induces high titers of anti-Su and autoantibodies in mice Quantitation by antigen capture ELISAs based on monospecific human autoimmune sera. Journal of Immunological Methods 182:1, pages 51-62.
Crossref
Toshimitsu Kawashima, Eugene G. Zappi, Tsu-San Lieu & Richard D Sontheimer. (2016) Impact of Ultraviolet Irradiation on Expression of SSA/Ro Autoantigenic Polypeptides in Transformed Human Epidermal Keratinocytes. Lupus 3:6, pages 493-500.
Crossref
Morris Reichlin, Antonio Brucato, Mark B. Frank, Peter J. Maddison, Victoria R. Mccubbin, Marianne Wolfson‐Reichlin & Lela A. Lee. (2005) Concentration of autoantibodies to native 60‐kd ro/ss‐a and denatured 52‐kd ro/ss‐a in eluates from the heart of a child who died with congenital complete heart block. Arthritis & Rheumatism 37:11, pages 1698-1703.
Crossref
E. ST. Williams Clair, James A. BurchJr.Jr. & Michael Saitta. (2005) Specificity of autoantibodies for recombinant 60–kd and 52–kd ro autoantigens. Arthritis & Rheumatism 37:9, pages 1373-1379.
Crossref
Lela A Lee, Mark Barton Frank, Victoria R McCubbin & Morris Reichlin. (1994) Autoantibodies of Neonatal Lupus Erythematosus. Journal of Investigative Dermatology 102:6, pages 963-966.
Crossref
Morris Reichlin. (1994) ANTIBODIES TO RIBONUCLEAR PROTEINS. Rheumatic Disease Clinics of North America 20:1, pages 29-43.
Crossref
Sylviane Muller. 1994. Autoimmunity: Experimental Aspects. Autoimmunity: Experimental Aspects 75 90 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.