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Molecular Membrane Biology Volume 30, 2013 - Issue 1
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Research Article

A systematic approach to the amplified expression, functional characterization and purification of inositol transporters from Bacillus subtilis

Kim E. BettaneyAstbury Centre for Structural Molecular Biology and School of Biomedical Sciences, University of Leeds, Leeds, UK
,
Preethi SukumarAstbury Centre for Structural Molecular Biology and School of Biomedical Sciences, University of Leeds, Leeds, UK
,
Rohanah HussainDiamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, UK
,
Giuliano SiligardiDiamond Light Source Ltd, Harwell Science and Innovation Campus, Didcot, Oxfordshire, UK
,
Peter J. F. HendersonAstbury Centre for Structural Molecular Biology and School of Biomedical Sciences, University of Leeds, Leeds, UK
&
Simon G. PatchingAstbury Centre for Structural Molecular Biology and School of Biomedical Sciences, University of Leeds, Leeds, UKCorrespondence[email protected]
Pages 3-14 | Received 04 May 2012, Accepted 10 Jul 2012, Published online: 18 Oct 2012

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Kazumi Hiruma-Shimizu, Hiroki Shimizu, Gary S. Thompson, Arnout P. Kalverda & Simon G. Patching. (2015) Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications. Molecular Membrane Biology 32:5-8, pages 139-155.
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Articles from other publishers (11)

Paul Ramp, Christopher Pfleger, Jonas Dittrich, Christina Mack, Holger Gohlke & Michael Bott. (2022) Physiological, Biochemical, and Structural Bioinformatic Analysis of the Multiple Inositol Dehydrogenases from Corynebacterium glutamicum. Microbiology Spectrum 10:5.
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Rohanah Hussain, Charlotte S. Hughes & Giuliano Siligardi. 2020. Targeting Enzymes for Pharmaceutical Development. Targeting Enzymes for Pharmaceutical Development 87 118 .
Giuliano SiligardiCharlotte S. HughesRohanah Hussain. (2018) Characterisation of sensor kinase by CD spectroscopy: golden rules and tips. Biochemical Society Transactions 46:6, pages 1627-1642.
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Lijie Sun, Hao Xie, Jean C. Ingram, Ryan J. Hope, Stephen A. Baldwin & Simon G. Patching. (2017) Retraction of “Characterization of the Escherichia coli Concentrative Nucleoside Transporter NupC Using Computational, Biochemical, and Biophysical Methods” . Biochemistry 57:28, pages 4237-4237.
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Irshad Ahmad, Nighat Nawaz, Nizam M. Darwesh, Sadeeq ur Rahman, Mohammad Z. Mustafa, Sher B. Khan & Simon G. Patching. (2018) Overcoming challenges for amplified expression of recombinant proteins using Escherichia coli. Protein Expression and Purification 144, pages 12-18.
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Moazur Rahman, Fouzia Ismat, Li Jiao, Jocelyn M. Baldwin, David J. Sharples, Stephen A. Baldwin & Simon G. Patching. (2016) Characterisation of the DAACS Family Escherichia coli Glutamate/Aspartate-Proton Symporter GltP Using Computational, Chemical, Biochemical and Biophysical Methods. The Journal of Membrane Biology 250:2, pages 145-162.
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Rohanah HussainStephen E. HardingCharlotte S. HughesPikyee MaSimon G. PatchingShalini EdaraGiuliano SiligardiPeter J.F. HendersonMary K. Phillips-Jones. (2016) Purification of bacterial membrane sensor kinases and biophysical methods for determination of their ligand and inhibitor interactions. Biochemical Society Transactions 44:3, pages 810-823.
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Pikyee Ma, Simon G. Patching, Ekaterina Ivanova, Jocelyn M. Baldwin, David Sharples, Stephen A. Baldwin & Peter J. F. Henderson. (2016) Allantoin transport protein, PucI, from Bacillus subtilis: evolutionary relationships, amplified expression, activity and specificity. Microbiology 162:5, pages 823-836.
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Rohanah Hussain & Giuliano Siligardi. 2016. The Next Generation in Membrane Protein Structure Determination. The Next Generation in Membrane Protein Structure Determination 43 59 .
Xinyi Huang & Marcy Hernick. (2015) Recombinant Expression of a Functional Myo-Inositol-1-Phosphate Synthase (MIPS) in Mycobacterium smegmatis. The Protein Journal 34:5, pages 380-390.
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Giuliano Siligardi, Rohanah Hussain, Simon G. Patching & Mary K. Phillips-Jones. (2014) Ligand- and drug-binding studies of membrane proteins revealed through circular dichroism spectroscopy. Biochimica et Biophysica Acta (BBA) - Biomembranes 1838:1, pages 34-42.
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