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Original Article

Stabilization of Immobilized Enzymes Against Water-Soluble Organic Cosolvents and Generation of Hyper-Hydrophilic Micro-Environments Surrounding Enzyme Molecules

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Pages 489-503 | Published online: 11 Jul 2009

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Carla Silva, Madalena Martins, Su Jing, Jiajia Fu & Artur Cavaco-Paulo. (2018) Practical insights on enzyme stabilization. Critical Reviews in Biotechnology 38:3, pages 335-350.
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Rafael C. Rodrigues, Ángel Berenguer-Murcia & Roberto Fernandez-Lafuente. (2011) Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes. Advanced Synthesis & Catalysis 353:13, pages 2216-2238.
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Zaida Cabrera, Melissa L.E. Gutarra, Jose M. Guisan & Jose M. Palomo. (2010) Highly enantioselective biocatalysts by coating immobilized lipases with polyethyleneimine. Catalysis Communications 11:11, pages 964-967.
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Akhilesh Kumar Shakya, Haider Sami, Akshay Srivastava & Ashok Kumar. (2010) Stability of responsive polymer–protein bioconjugates. Progress in Polymer Science 35:4, pages 459-486.
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Michael C. FlickingerJose M. Guisan, Lorena Betancor & Gloria Fernandez-Lorente. 2009. Encyclopedia of Industrial Biotechnology. Encyclopedia of Industrial Biotechnology.
Masahiro Yasuda, Hibiki Nikaido, Wilhelm R. Glomm, Hiroyasu Ogino, Kosaku Ishimi & Haruo Ishikawa. (2009) Enzyme immobilization on amphiphilic polymer particles having grafted polyionic polymer chains. Biochemical Engineering Journal 48:1, pages 6-12.
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Zaida Cabrera, Gloria Fernandez-Lorente, Roberto Fernandez-Lafuente, Jose M. Palomo & Jose M. Guisan. (2009) Enhancement of Novozym-435 catalytic properties by physical or chemical modification. Process Biochemistry 44:2, pages 226-231.
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Gabriela Irazoqui, Cecilia Giacomini, Francisco Batista-Viera & Beatriz M. Brena. (2007) Hydrophilization of immobilized model enzymes suggests a widely applicable method for enhancing protein stability in polar organic co-solvents. Journal of Molecular Catalysis B: Enzymatic 46:1-4, pages 43-51.
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Tamara Montes, Valeria Grazú, Isabel Manso, Beatriz Galán, Fernando López‐Gallego, Ramón González, Juan A. Hermoso, José L. García, José M. Guisán & Roberto Fernández‐Lafuente. (2007) Improved Stabilization of Genetically Modified Penicillin G Acylase in the Presence of Organic Cosolvents by Co‐ Immobilization of the Enzyme with Polyethyleneimine. Advanced Synthesis & Catalysis 349:3, pages 459-464.
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Lorena Wilson, Gloria Fernández-Lorente, Roberto Fernández-Lafuente, Andrés Illanes, José M. Guisán & José M. Palomo. (2006) CLEAs of lipases and poly-ionic polymers: A simple way of preparing stable biocatalysts with improved properties. Enzyme and Microbial Technology 39:4, pages 750-755.
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Bettina Brandt, Aurelio Hidalgo & Uwe T. Bornscheuer. (2006) Immobilization of enzymes in microtiter plate scale. Biotechnology Journal 1:5, pages 582-587.
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Cesar Mateo, Bruno Fernandes, Fred van Rantwijk, Andreas Stolz & Roger A. Sheldon. (2006) Stabilisation of oxygen-labile nitrilases via co-aggregation with poly(ethyleneimine). Journal of Molecular Catalysis B: Enzymatic 38:3-6, pages 154-157.
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Roberto C. Giordano, Marcelo P.A. Ribeiro & Raquel L.C. Giordano. (2006) Kinetics of β-lactam antibiotics synthesis by penicillin G acylase (PGA) from the viewpoint of the industrial enzymatic reactor optimization. Biotechnology Advances 24:1, pages 27-41.
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A. Illanes, C. Altamirano, M. Fuentes, F. Zamorano & C. Aguirre. (2005) Synthesis of cephalexin in organic medium at high substrate concentrations and low enzyme to substrate ratio. Journal of Molecular Catalysis B: Enzymatic 35:1-3, pages 45-51.
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A Illanes, M.S Anjarı́, C Altamirano & C Aguirre. (2004) Optimization of cephalexin synthesis with immobilized penicillin acylase in ethylene glycol medium at low temperatures. Journal of Molecular Catalysis B: Enzymatic 30:2, pages 95-103.
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Lorena Wilson, Andrés Illanes, Benevides C. C. Pessela, Olga Abian, Roberto Fernández‐Lafuente & José M. Guisán. (2004) Encapsulation of crosslinked penicillin G acylase aggregates in lentikats: Evaluation of a novel biocatalyst in organic media. Biotechnology and Bioengineering 86:5, pages 558-562.
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Lorena Wilson, Andrés Illanes, Olga Abián, Benevides C. C. Pessela, Roberto Fernández-Lafuente & José M. Guisán. (2004) Co-Aggregation of Penicillin G Acylase and Polyionic Polymers:  An Easy Methodology To Prepare Enzyme Biocatalysts Stable in Organic Media. Biomacromolecules 5:3, pages 852-857.
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Olga Abian, César Mateo, Gloria Fernández-Lorente, José Manuel Guisán & Roberto Fernández-Lafuente. (2008) Thermodynamically Controlled Synthesis of Amide Bonds Catalyzed by Highly Organic Solvent-Resistant Penicillin Acylase Derivatives. Biotechnology Progress 20:1, pages 117-121.
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Dong-Zhi Wei & Liu Yang. (2003) Effects of ethylene glycol on the synthesis of ampicillin using immobilized penicillin G acylase. Journal of Chemical Technology & Biotechnology 78:4, pages 431-436.
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