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DNA Sequence Volume 2, 1992 - Issue 5
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Original Article

DNA sequencing by a subcloning-walking strategy using a specific and semi-random primer in the polymerase chain reaction

P. Verhasselt Laboratory of Gene Technology, University of Leuven, W. de Croylaan 42, B-3001, Leuven, Belgium
,
M. Voet Laboratory of Gene Technology, University of Leuven, W. de Croylaan 42, B-3001, Leuven, Belgium
&
G. Volckaert Laboratory of Gene Technology, University of Leuven, W. de Croylaan 42, B-3001, Leuven, Belgium
Pages 281-287 | Published online: 11 Jul 2009

Citations (11)

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Read on this site (1)

P. Verhasselt, S. Van Campenhout, M. B. Wellens & G. Volckaert. (1993) A general method for routine sequencing of cloned DNA fragments using commercial dye primers and its application in sequence analysis of Toxoplasma gondii RH genome fragments. DNA Sequence 4:2, pages 71-77.
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Articles from other publishers (10)

Cristiana Ossaille Beltrame, Marina Farrel Côrtes, Raquel Regina Bonelli, Ana Beatriz de Almeida Côrrea, Ana Maria Nunes Botelho, Marco Antônio Américo, Sérgio Eduardo Longo Fracalanzza & Agnes Marie Sá Figueiredo. (2015) Inactivation of the Autolysis-Related Genes lrgB and yycI in Staphylococcus aureus Increases Cell Lysis-Dependent eDNA Release and Enhances Biofilm Development In Vitro and In Vivo. PLOS ONE 10:9, pages e0138924.
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Aleksandar Milosavljevic, Miklós Csürös, George Weinstock & Richard A. Gibbs. (2003) Shotgun sequencing, clone pooling, and comparative strategies for mapping and sequencing. TARGETS 2:6, pages 245-252.
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Guido Volckaert, Peter Verhasselt, Marleen Voet & Johan Robben. 2001. Biotechnology Set. Biotechnology Set 257 315 .
P. Van As, Winnie Janssens, O.M. Onagbesan, Veerle Bruggeman, Nadine Buys, J. Sanders, S. Van Der Geyten, Veerle M. Darras & E. Decuypere. (2001) Quantification of Growth Hormone Receptor Extra- and Intracellular Domain Gene Expression in Chicken Liver by Quantitative Competitive RT–PCR. General and Comparative Endocrinology 122:2, pages 213-224.
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M. Safi, N. Buys, O.M. Onagbesan, B. Vleugels & E. Decuypere. (1998) Quantification of Inhibin/Activin α and βA Subunit Messenger Ribonucleic Acid by Competitive Reverse Transcription-Polymerase Chain Reaction in Chicken Granulosa Cells during Follicular Development. Biology of Reproduction 59:5, pages 1047-1054.
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Fredrik Sterky, Anders Holmberg, Gunnar Alexandersson, Joakim Lundeberg & Mathias Uhlén. (1998) Direct sequencing of bacterial artificial chromosomes (BACs) and prokaryotic genomes by biotin-capture PCR. Journal of Biotechnology 60:1-2, pages 119-129.
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Peter Verhasselt, Rita Aert, Marleen Voet & Guido Volckaert. (1994) XIV. Yeast sequencing reports. Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence. Yeast 10:7, pages 945-951.
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André Rosenthal, Matthias Platzer & D. Stephen Charnock-Jones. 1994. The Polymerase Chain Reaction. The Polymerase Chain Reaction 222 229 .
Peter Verhasselt, Marie-Jeanne Reekmans & Guido Volckaert. (1993) Open reading frame analysis by selective PCR-mediated deletion mutagenesis. Genetic Analysis: Biomolecular Engineering 10:1, pages 16-23.
Crossref
Guido Volckaert, Peter Verhasselt, Marleen Voet & Johan Robben. 1992. Biotechnology. Biotechnology 257 315 .

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