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Stain Technology Volume 47, 1972 - Issue 5
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Original Article

The Use of p-Phenylenediamine in the Block to Enhance Osmium Staining for Electron Microscopy

Janet M. Ledingham Wellcome Medical Research Institute, Department of Medicine, University of Otago Medical School, Dunedin, New Zealand
&
F. O. Simpson Wellcome Medical Research Institute, Department of Medicine, University of Otago Medical School, Dunedin, New Zealand
Pages 239-243 | Published online: 12 Jul 2009

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Iezo Nakao, Hiyoshi Okada, Hideto Senzaki, Rieko Nishimura, Naoki Fujita, Nobuaki Shikata & Sotokichi Morii. (1992) A Mixture of Paraphenylenediamine and Imidazole: Its Effect on the Extraction of Lipid Droplets during Electron Microscopy Staining. Biotechnic & Histochemistry 67:4, pages 219-223.
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Arya K. Bal. (1990) Localization of Plant Lipids for Light Microscopy Using P-Phenylenediamine in Tissues of Arachis Hypogaea L.. Stain Technology 65:2, pages 91-94.
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EricC. Henry. (1987) Primitive reproductive characters and a photoperiodic response in Saccorhiza dermatodea (Laminariales, Phaeophyceae). British Phycological Journal 22:1, pages 23-31.
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D. P. Boshier, Hilary Holloway & Lynette F. Kitchin. (1984) A Comparison of Standard Lipid Staining Techniques Used in Electron Microscopic Studies of Mammalian Tissues. Stain Technology 59:2, pages 83-89.
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Nora Shepard, Edgard E. Delvin & Nelson Mitchell. (1981) Ruthenium Red—p-Phenylenediamine Staining of Monolayers to Facilitate Handling and Selection of Specific Cells for Transmission Electron Microscopy. Stain Technology 56:3, pages 143-147.
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Articles from other publishers (34)

Ahmed F Alkandari, Abrar A Alawadhi, Fatma A Alawadhi, Alyaa Mousa & Sampath Madhyastha. (2023) Erythematous Linear Lesion on the Course of Superficial Fibular Nerve After the Topical Application of Black Henna: A Case Report. Cureus.
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Shingo Kiyoto, Takuji Ichino, Tatsuya Awano & Kazufumi Yazaki. (2022) Improved chemical fixation of lipid-secreting plant cells for transmission electron microscopy. Microscopy 71:4, pages 206-213.
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Norimitsu Shirai, Frank J. Geoly, Walter F. Bobrowski & Carlin Okerberg. (2016) The Application of Paraphenylenediamine Staining for Assessment of Phospholipidosis. Toxicologic Pathology 44:8, pages 1160-1165.
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Basant Kumar Mohanty & Brij L. Gupta. (2011) A marked animal–vegetal polarity in the localization of Na + ,K + ‐ATPase activity and its down‐regulation following progesterone‐induced maturation . Molecular Reproduction and Development 79:2, pages 138-160.
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Ralf M. Schweiggert, Christof B. Steingass, Annerose Heller, Patricia Esquivel & Reinhold Carle. (2011) Characterization of chromoplasts and carotenoids of red- and yellow-fleshed papaya (Carica papaya L.). Planta 234:5, pages 1031-1044.
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E. Ann Ellis. (2010) p -Phenylenediamine: An Adjunct to and a Substitute for Osmium Tetroxide . Microscopy Today 18:5, pages 48-50.
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Bernadette F. Ardelli & Patrick T. K. Woo. (2001) THE IN VITRO EFFECTS OF ISOMETAMIDIUM CHLORIDE (SAMORIN) ON THE PISCINE HEMOFLAGELLATE CRYPTOBIA SALMOSITICA (KINETOPLASTIDA, BODONINA) . Journal of Parasitology 87:1, pages 194-202.
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J.M.P Mouveroux, M Verkijk, E.A.J.F Lakke & E Marani. (2000) Intrinsic properties of the developing motor cortex in the rat: in vitro axons from the medial somatomotor cortex grow faster than axons from the lateral somatomotor cortex. Developmental Brain Research 122:1, pages 59-66.
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H.K. Abbas, R.N. Paul, R.T. Riley, T. Tanaka & W.T. Shier. (1998) Ultrastructural effects of AAL-toxin TA from the fungus Alternaria alternata on black nightshade (Solanum nigrum L.) leaf discs and correlation with biochemical measures of toxicity. Toxicon 36:12, pages 1821-1832.
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Michel Goldberg & Adele L. Boskey. (1996) Lipids and Biomineralizations. Progress in Histochemistry and Cytochemistry 31:2, pages III-187.
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L. Vermelin, D. Septier & M. Goldberg. (1994) Iodopolatinate visualization of phospholipids in rat incisor predentine and dentine, compared with malachite green aldehyde. Histochemistry 101:1, pages 63-72.
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S.‐Y. LEE & C.V. MORR. (2006) Fixation Staining Methods for Examining Microstructure in Whipped Cream by Electron Microscopy. Journal of Food Science 58:1, pages 124-127.
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R. N. Paul & G. H. Egley. (2020) Cuticular penetration of Sesbania exaltata STEM tissue by penetration pegs from appressoria of the plant pathogenic fungus: Colletotrichum truncatum . Proceedings, annual meeting, Electron Microscopy Society of America 50:1, pages 838-839.
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S.‐Y. LEE & C.V. MORR. (2006) Fixation and Staining Milkfat Globules in Cream for Transmission and Scanning Electron Microscopy. Journal of Food Science 57:4, pages 887-891.
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Gary F. Joye & Rex N. Paul. (2017) Histology of Infection of Hydrilla verticillata by Macrophomina phaseolina . Weed Science 40:2, pages 288-295.
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Jean-Rapha�l Nefussi, Dominique Septier, Jean-Michel Sautier, Nadine Forest & Michel Goldberg. (1992) Localization of malachite green positive lipids in the matrix of bone nodule formed in vitro. Calcified Tissue International 50:3, pages 273-282.
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L. Tetley & K. Vickerman. (1991) The glycosomes of trypanosomes: number and distribution as revealed by electron spectroscopic imaging and 3-D reconstruction. Journal of Microscopy 162:1, pages 83-90.
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S. JAYARAM & A. K. BAL. (2006) Oleosomes (lipid bodies) in nitrogen‐fixing peanut nodules. Plant, Cell & Environment 14:2, pages 195-203.
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R. N. Paul & C. G. McWhorter. (2020) Correlation of ultrastructure with the mechanism of wax filament production in cork cells in Johnsongrass [ Sorghum halepense (L.) Pers] . Proceedings, annual meeting, Electron Microscopy Society of America 48:3, pages 676-677.
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M. Zaczek & T. W. Keenan. (1990) Morphological evidence for an endoplasmic reticulum origin of milk lipid globules obtained using lipid-selective staining procedures. Protoplasma 159:2-3, pages 179-183.
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. 1986. Basic Techniques for Transmission Electron Microscopy. Basic Techniques for Transmission Electron Microscopy 381 400 .
. (2016) 6 Final Report on the Safety Assessment of p -Phenylenediamine . Journal of the American College of Toxicology 4:3, pages 203-266.
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M. Goldberg & D. Septier. (1985) Improved lipid preservation by malachite green-glutaraldehyde fixation in rat incisor predentine and dentine. Archives of Oral Biology 30:10, pages 717-726.
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Ondina Colman & Juan C. Stockert. (1983) Observations on the osmium tetroxide-p-phenylenediamine staining method for Epon semithin sections of nervous tissue. Acta Histochemica 72:1, pages 123-125.
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M. Goldberg, M. Lelous, F. Escaig & M. Boudin. (1983) Lipids in the developing enamel of the rat incisor. Histochemistry 78:2, pages 145-156.
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D. S. Ellis, D. A. Evans & Susan Stamford. (1981) A brief note on an arrangement of sub-pellicular tubules in trypanosomes. Zeitschrift f�r Parasitenkunde Parasitology Research 65:3, pages 361-363.
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N. Shepard & N. Mitchell. (1981) Acridine orange stabilization of glycosaminoglycans in beginning endochondral ossification. Histochemistry 70:2, pages 107-114.
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C.N.A. Trotman, B.C. Mansfield & W.P. Tate. (1980) Inhibition of emergence, hatching, and protein biosynthesis in embryonic Artemia salina. Developmental Biology 80:1, pages 167-174.
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David E. Allen & Douglas D. Perrin. 1979. 63 84 .
B.J. Ward, J.A. Gloster & P. Harris. (1979) The incorporation and distribution of 3H oleic acid in the isolated perfused guinea-pig heart: A biochemical and EM autoradiographic study. Tissue and Cell 11:4, pages 793-801.
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Heinz Popiela. (1976) In vivo limb tissue development in the absence of nerves: A quantitative study. Experimental Neurology 53:1, pages 214-226.
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Heinz Popiela. (2005) Muscle satellite cells in urodele amphibians: Facilitated identification of satellite cells using ruthenium red staining. Journal of Experimental Zoology 198:1, pages 57-64.
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Barbara J. Ward & Josephine A. Gloster. (2011) Lipid losses during processing of cardiac muscle for electron microscopy. Journal of Microscopy 108:1, pages 41-50.
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L.T. Threadgold. (1976) Fasciola hepatica: Ultrastructure and histochemistry of the glycocalyx of the tegument. Experimental Parasitology 39:1, pages 119-134.
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