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Stain Technology Volume 50, 1975 - Issue 6
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Original Article

The Purification of Methylene Blue and Azure B by Solvent Extraction and Crystallization

P. N. Marshall Department of Haematology, Royal Postgraduate Medical School, London, W12 OHS, England
&
S. M. Lewis Department of Haematology, Royal Postgraduate Medical School, London, W12 OHS, England
Pages 375-381 | Published online: 12 Jul 2009

Citations (19)

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Read on this site (3)

CJ Cooksey. (2017) Quirks of dye nomenclature. 8. Methylene blue, azure and violet. Biotechnic & Histochemistry 92:5, pages 347-356.
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Angelo Russo, Patricia T. Donaldson & R. D. Lillie. (1978) Lower Azure B Methylene Blue Ratios in Giemsa Type Blood and Malaria Stains. Stain Technology 53:1, pages 37-41.
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R. D. Lillie. (1978) Romanowsky–Malachowski Stains the So-Called Romanowsky Stain: Malachowski's 1891 Use of Alkali Polychromed Methylene Blue for Malaria Plasmodia. Stain Technology 53:1, pages 23-28.
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Articles from other publishers (16)

Dipayan Das, Debasmita Ghosh & Palash Mandal. (2021) Preservative potential of biosynthesized silver nanoparticles: prevention of xylem occlusion and microbial proliferation at postharvest stage of preservation. Environmental Science and Pollution Research 28:17, pages 22038-22063.
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Andrew Mills. (2012) An overview of the methylene blue ISO test for assessing the activities of photocatalytic films. Applied Catalysis B: Environmental 128, pages 144-149.
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Andrew Mills, David Hazafy, John Parkinson, Tell Tuttle & Michael G. Hutchings. (2011) Effect of alkali on methylene blue (C.I. Basic Blue 9) and other thiazine dyes. Dyes and Pigments 88:2, pages 149-155.
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Andrew MillsDavid HazafyJohn A. ParkinsonTell TuttleMichael G. Hutchings. (2009) Comment on “Solvent Effect on the Electronic Spectra of Azine Dyes under Alkaline Condition”. The Journal of Physical Chemistry A 113:34, pages 9575-9576.
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B. M. Van Liedekerke, H. J. Nelis, D. H. Wittekind & A. P. De Leenheer. (1991) Stability study of Azure B, Eosin Y and commercial Romanowsky Giemsa stock solutions using high performance liquid chromatography. The Histochemical Journal 23:4, pages 189-195.
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D. H�glin, W. Seiffert & H. W. Zimmermann. (1986) Spektroskopische und thermodynamische Untersuchungen zur Bindung von Azur B an Chondroitinsulfat und zur Bindungsgeometrie des metachromatischen FarbstoffkomplexesSpectroscopic and thermodynamic investigations on the binding of azure B to chondroitin sulfate and the structure of the metachromatic dye complex. Histochemistry 86:1, pages 71-82.
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Kevin D. Barron, Charlotte M. McGuinness, Louis J. Misantone, M. F. Zanakis, Bernice Grafstein & Marion Murray. (1985) RNA content of normal and axotomized retinal ganglion cells of rat and goldfish. The Journal of Comparative Neurology 236:2, pages 265-273.
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. 1982. Histochemistry. Histochemistry 260 283 .
E. Zipfel, J.-R. Grezes, W. Seiffert & H. W. Zimmermann. (1981) �ber Romanowsky-Farbstoffe und den Romanowsky-Giemsa-EffektRomanowsky dyes and romanowsky-giemsa effect. Histochemistry 72:2, pages 279-290.
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D. WITTEKIND. (2008) On the nature of Romanowsky dyes and the Romanowsky-Giemsa effect. Clinical & Laboratory Haematology 1:4, pages 247-262.
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P. N. Marshall. (1979) Commercially available ?pure? Azure dyes ? caveat emptor. The Histochemical Journal 11:4, pages 489-493.
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P N Marshall, S A Bentley & S M Lewis. (1978) Staining properties and stability of a standardised Romanowsky stain.. Journal of Clinical Pathology 31:3, pages 280-282.
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P. N. Marshall, S. A. Bentley & S. M. Lewis. (2009) Standardization of Romanowsky Stains. Scandinavian Journal of Haematology 20:3, pages 206-212.
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P. N. Marshall. (1978) Romanowsky-type stains in haemotology. The Histochemical Journal 10:1, pages 1-29.
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P N Marshall, S A Bentley & S M Lewis. (1976) Purified azure B as a reticulocyte stain.. Journal of Clinical Pathology 29:12, pages 1060-1063.
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P. N. Marshall. (1976) The composition of stains produced by the oxidation of Methylene Blue. The Histochemical Journal 8:4, pages 431-442.
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