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Original Article

Staining Air Dried Protoplasts for Study of Plant Chromosomes

Pages 101-106 | Published online: 12 Jul 2009

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L. O. Tacuatiá, E. Kaltchuk-Santos, T. T. Souza-Chies, L. Eggers, E. R. Forni-Martins, F. Pustahija, O. Robin & S. Siljak-Yakovlev. (2017) Physical mapping of 35S rRNA genes and genome size variation in polyploid series of Sisyrinchium micranthum and S. rosulatum (Iridaceae: Iridoideae). Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 151:3, pages 403-413.
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JM Rodríguez-Domínguez, LL Ríos-Lara, E Tapia-Campos & R Barba-Gonzalez. (2017) An improved technique for obtaining well-spread metaphases from plants with numerous large chromosomes. Biotechnic & Histochemistry 92:3, pages 159-166.
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Akio Kato. (1999) Air drying method using nitrous oxide for chromosome counting in maize. Biotechnic & Histochemistry 74:3, pages 160-166.
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Carlos Roberto De Carvalho & Luiz Sérgio Saraiva. (1993) An Air Drying Technique for Maize Chromosomes without Enzymatic Maceration. Biotechnic & Histochemistry 68:3, pages 142-145.
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Articles from other publishers (19)

Pilar Prieto & Tomás Naranjo. 2020. Plant Meiosis. Plant Meiosis 141 168 .
Juliana Lustosa Matos de Alencar, Eliane Kaltchuk-Santos, Juliana Fachinetto, Luana Olinda Tacuatiá, Eliana Regina Forni-Martins, Eudes Maria Stiehl-Alves & Tatiana Teixeira de Souza-Chies. (2018) Genetic and ecological niche modeling of Calydorea crocoides (Iridaceae): an endemic species of Subtropical Highland Grasslands. Genetics and Molecular Biology 41:1 suppl 1, pages 327-340.
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Yi-Tzu Kuo, Hui-Lan Hsu, Chih-Hsin Yeh & Song-Bin Chang. (2016) Application of a modified drop method for high-resolution pachytene chromosome spreads in two Phalaenopsis species. Molecular Cytogenetics 9:1.
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Haoyang Xin, Yue Lan, Jisen Shi, Yan Ma & Mengli Xi. (2016) Pachytene Chromosome Preparation in Populus deltoides Marsh . Current Protocols in Plant Biology 1:4, pages 566-573.
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. 2016. Plant Cytogenetics. Plant Cytogenetics 457 514 .
Ilya Kirov, Mikhail Divashuk, Katrijn Van Laere, Alexander Soloviev & Ludmila Khrustaleva. (2014) An easy “SteamDrop” method for high quality plant chromosome preparation. Molecular Cytogenetics 7:1.
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W. R. Clarindo & C. R. Carvalho. (2008) First Coffea arabica karyogram showing that this species is a true allotetraploid. Plant Systematics and Evolution 274:3-4, pages 237-241.
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Wellington Ronildo Clarindo & Carlos Roberto Carvalho. (2006) A High Quality Chromosome Preparation from Cell Suspension Aggregates Culture of Coffea canephora. CYTOLOGIA 71:3, pages 243-249.
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Ram Singh. 2002. Plant Cytogenetics, Second Edition. Plant Cytogenetics, Second Edition 385 442 .
M. Murata, N. Nakata & Y. Yasumuro. (1992) Origin and molecular structure of a midget chromosome in a common wheat carrying rye cytoplasm. Chromosoma 102:1, pages 27-31.
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Matthew C. Griffor, Lila O. Vodkin, Ram J. Singh & Theodore Hymowitz. (1991) Fluorescentin situ hybridization to soybean metaphase chromosomes. Plant Molecular Biology 17:1, pages 101-109.
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K. Vamling & K. Glimelius. 1990. Legumes and Oilseed Crops I. Legumes and Oilseed Crops I 385 417 .
M. Murata. (1989) Effects of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.). Theoretical and Applied Genetics 78:4, pages 521-524.
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Pung-Ling Huang, Klaus Hahlbrock & Imre E. Somssich. (1988) Detection of a single-copy gene on plant chromosomes by in situ hybridization. Molecular and General Genetics MGG 211:1, pages 143-147.
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G�nter Geber & Dieter Schweizer. (1987) Cytochemical heterochromatin differentiation inSinapis alba (Cruciferae) using a simple air-drying technique for producing chromosome spreads. Plant Systematics and Evolution 158:2-4, pages 97-106.
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L. P. Pijnacker, J. H. M. Hermelink & M. A. Ferwerda. (1986) Variability of DNA content and karyotype in cell cultures of an interdihaploid Solanum tuberosum. Plant Cell Reports 5:1, pages 43-46.
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T.J. Orton. 1986. Plant Regeneration and Genetic Variability. Plant Regeneration and Genetic Variability 345 366 .
J. F. Jackson. 1985. Cell Components. Cell Components 353 374 .
Alan R. Gould. 1984. Laboratory Procedures and their Applications. Laboratory Procedures and their Applications 698 711 .

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