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Original Article

Detection of a Stable Free Radical in the B2 Subunit of the Manganese Ribonucleotide Reductase (MN-RRase) of Corynebacterium ammoniagenes

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Pages 473-481 | Received 19 Oct 1995, Published online: 07 Jul 2009

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Bouziane Abbouni, Wulf Oehlmann, Patrick Stolle, Antonio J. Pierik & Georg Auling. (2009) Electron paramagnetic resonance (EPR) spectroscopy of the stable-free radical in the native metallo-cofactor of the manganese-ribonucleotide reductase (Mn-RNR) of Corynebacterium glutamicum. Free Radical Research 43:10, pages 943-950.
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Joseph A. Cotruvo, Jr & JoAnne Stubbe. (2012) Metallation and mismetallation of iron and manganese proteins in vitro and in vivo: the class I ribonucleotide reductases as a case study. Metallomics 4:10, pages 1020.
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Martin Högbom. (2011) Metal use in ribonucleotide reductase R2, di-iron, di-manganese and heterodinuclear—an intricate bioinorganic workaround to use different metals for the same reaction. Metallomics 3:2, pages 110.
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Patrick Stolle, Olaf Barckhausen, Wulf Oehlmann, Nadine Knobbe, Carla Vogt, Antonio J. Pierik, Nicholas Cox, Peter P. Schmidt, Edward J. Reijerse, Wolfgang Lubitz & Georg Auling. (2010) Homologous expression of the nrdF gene of Corynebacterium ammoniagenes strain ATCC 6872 generates a manganese‐metallocofactor (R2F) and a stable tyrosyl radical (Y • ) involved in ribonucleotide reduction . The FEBS Journal 277:23, pages 4849-4862.
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Nicholas Cox, Hideaki Ogata, Patrick Stolle, Edward Reijerse, Georg Auling & Wolfgang Lubitz. (2010) A Tyrosyl−Dimanganese Coupled Spin System is the Native Metalloradical Cofactor of the R2F Subunit of the Ribonucleotide Reductase of Corynebacterium ammoniagenes. Journal of the American Chemical Society 132:32, pages 11197-11213.
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Pawel M. Kozlowski, Takashi Kamachi, Manoj Kumar, Tomonori Nakayama & Kazunari Yoshizawa. (2010) Theoretical Analysis of the Diradical Nature of Adenosylcobalamin Cofactor−Tyrosine Complex in B 12 -Dependent Mutases: Inspiring PCET-Driven Enzymatic Catalysis . The Journal of Physical Chemistry B 114:17, pages 5928-5939.
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Manoj Kumar & Pawel M. Kozlowski. (2009) Role of Tyrosine Residue in the Activation of Co−C Bond in Coenzyme B 12 -Dependent Enzymes: Another Case of Proton-Coupled Electron Transfer? . The Journal of Physical Chemistry B 113:27, pages 9050-9054.
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Pedro Alexandrino Fernandes & Maria João Ramos. (2003) Theoretical Studies on the Mechanism of Inhibition of Ribonucleotide Reductase by ( E )-2‘-Fluoromethylene-2‘-deoxycitidine-5‘-diphosphate . Journal of the American Chemical Society 125:20, pages 6311-6322.
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Fahmi Himo & Leif A. Eriksson. 2001. Theoretical Biochemistry - Processes and Properties of Biological Systems. Theoretical Biochemistry - Processes and Properties of Biological Systems 145 181 .
Yasmin Huque, Franck Fieschi, Eduard Torrents, Isidre Gibert, Rolf Eliasson, Peter Reichard, Margareta Sahlin & Britt-Marie Sjöberg. (2000) The Active Form of the R2F Protein of Class Ib Ribonucleotide Reductase from Corynebacterium ammoniagenesIs a Diferric Protein. Journal of Biological Chemistry 275:33, pages 25365-25371.
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Rafael Ruiz, Andrea Caneschi, Dante Gatteschi, Ana.B Gaspar, Jose A. Real, Isabel Fernandez & M Carmen Mu?oz. (1999) Manganese(III)-mediated oxidative carbon?carbon bond cleavage of the 1,10-phenanthroline-5,6-dione ligand. Inorganic Chemistry Communications 2:11, pages 521-523.
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Sergiu Gorun & Jan Wikaira. 1999. Bioinorganic Catalysis. Bioinorganic Catalysis 355 422 .
Stuart Licht & Joanne Stubbe. 1999. Comprehensive Natural Products Chemistry. Comprehensive Natural Products Chemistry 163 203 .
Thomas C. Brunold, Daniel R. Gamelin, Timothy L. Stemmler, Sanjay K. Mandal, William H. Armstrong, James E. Penner-Hahn & Edward I. Solomon. (1998) Spectroscopic Studies of Oxidized Manganese Catalase and μ-Oxo-Bridged Dimanganese(III) Model Complexes:  Electronic Structure of the Active Site and Its Relation to Catalysis. Journal of the American Chemical Society 120:34, pages 8724-8738.
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Franck Fieschi, Eduard Torrents, Larisa Toulokhonova, Albert Jordan, Ulf Hellman, Jordi Barbe, Isidre Gibert, Margareta Karlsson & Britt-Marie Sjöberg. (1998) The Manganese-containing Ribonucleotide Reductase ofCorynebacterium ammoniagenes Is a Class Ib Enzyme. Journal of Biological Chemistry 273:8, pages 4329-4337.
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Neil A. Law, M. Tyler Caudle & Vincent L. Pecoraro. 1998. 305 440 .
S. F. Mohamed, O. R. Gvozdiak, D. Stallmann, U. Griepenburg, H. Follmann & G. Auling. (2008) Ribonucleotide reductase in Bacillus subtilis — evidence for a Mn‐dependent enzyme. BioFactors 7:4, pages 337-344.
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B. -M. Sjöberg. 1997. Metal Sites in Proteins and Models. Metal Sites in Proteins and Models 139 173 .

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