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Journal of Receptors and Signal Transduction Volume 17, 1997 - Issue 1-3
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Original Article

Expression of Ligand-Gated Ion Channels with the Semliki Forest Virus Expression System

Kenneth Lundstrom Glaxo Wellcome, Medicines Research Centre, Stevenage, Herts, SG1 2NY, UK; Glaxo Institute for Molecular Biology, Geneva, Switzerland
,
Anton Michel Glaxo Institute for Applied Pharmacology, Cambridge, UK
,
Horst Blasey Glaxo Institute for Molecular Biology, Geneva, Switzerland
,
Alain R. Bernard Glaxo Institute for Molecular Biology, Geneva, Switzerland
,
Ruud Hovius Institute of Physical Chemistry IV, Chemistry Department, Swiss Federal Institute of Technology, CH-1015, Lausanne, Switzerland
,
Horst Vogel Institute of Physical Chemistry IV, Chemistry Department, Swiss Federal Institute of Technology, CH-1015, Lausanne, Switzerland
&
Annmarie Surprenant Glaxo Institute for Molecular Biology, Geneva, Switzerland
 show all
Pages 115-126 | Published online: 26 Jun 2009

Citations (29)

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Read on this site (4)

Kenneth Lundstrom. (2002) SEMLIKI FOREST VIRUS-BASED EXPRESSION FOR VERSATILE USE IN RECEPTOR RESEARCH. Journal of Receptors and Signal Transduction 22:1-4, pages 229-240.
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Kenneth Lundstrom. (1999) Alphaviruses as Tools in Neurobiology and Gene Therapy. Journal of Receptors and Signal Transduction 19:1-4, pages 673-686.
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Ruud Hovius, Evelyne L. Schmid, Ana-Paula Tairi, Horst Blasey, Alain R. Bernard, Kenneth Lundström & Horst Vogel. (1999) Fluorescence Techniques for Fundamental and Applied Studies of Membrane Protein Receptors: The 5-HT, Serotonin Receptor. Journal of Receptors and Signal Transduction 19:1-4, pages 533-545.
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A. Scheer, K. Björ Klöf, S. Cotecchia & K. Lundstrom. (1999) Expression of the α1b-Adrenergic Receptor and G Protein Subunits in Mammalian Cell Lines Using the Semliki Forest Virus Expression System. Journal of Receptors and Signal Transduction 19:1-4, pages 369-378.
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Articles from other publishers (25)

Deniz B. Hizal, Erika Ohsfeldt, Sunny Mai & Michael J. Betenbaugh. 2011. Production of Membrane Proteins. Production of Membrane Proteins 139 165 .
Kenneth Lundstrom. 2010. Heterologous Expression of Membrane Proteins. Heterologous Expression of Membrane Proteins 149 163 .
Isabelle Mus-Veteau. 2010. Heterologous Expression of Membrane Proteins. Heterologous Expression of Membrane Proteins 1 16 .
Jeffrey J. Clare. 2008. Protein Science Encyclopedia. Protein Science Encyclopedia.
K. Lundstrom. (2007) Structural genomics and drug discovery. Journal of Cellular and Molecular Medicine 11:2, pages 224-238.
Crossref
R. J. E. Derks, T. Letzel, C. F. Jong, A. Marle, H. Lingeman, R. Leurs & H. Irth. (2006) SEC–MS as an Approach to Isolate and Directly Identifying Small Molecular GPCR–Ligands from Complex Mixtures Without Labeling. Chromatographia 64:7-8, pages 379-385.
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Lidia Ivanova, Johanna Brändli, Philippe Saudan & Martin F. Bachmann. (2005) Hybrid Sindbis/Epstein-Barr virus episomal expression vector for inducible production of proteins. BioTechniques 39:2, pages 209-212.
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Seema S. Gangolli, Nikolaos Vasilakis, Gerald R. Kovacs, Timothy J. Zamb & Jacek Kowalski. (2003) A method of alphavirus replicon particle titration based on expression of functional replicase/transcriptase. Journal of Virological Methods 109:2, pages 133-138.
Crossref
Kenneth Lundstrom. (2003) Semliki Forest virus vectors for rapid and high-level expression of integral membrane proteins. Biochimica et Biophysica Acta (BBA) - Biomembranes 1610:1, pages 90-96.
Crossref
Kenneth Lundstrom. 2003. Gene Transfer and Expression in Mammalian Cells. Gene Transfer and Expression in Mammalian Cells 207 230 .
S Sen, V.-P Jaakola, H Heimo, P Kivelä, M Scheinin, K Lundstrom & A Goldman. (2002) Development of a scintiplate assay for recombinant human α2B-adrenergic receptor. Analytical Biochemistry 307:2, pages 280-286.
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H. D. Blasey, B. Brethon, R. Hovius, K. lundström, L. Rey, H. Vogel, A. -P. Tair & A. R. Bernard. 2002. New Developments and New Applications in Animal Cell Technology. New Developments and New Applications in Animal Cell Technology 449 455 .
Petra Meissner, Horst Pick, Alexandra Kulangara, Philippe Chatellard, Kirstin Friedrich & Florian M. Wurm. (2001) Transient gene expression: Recombinant protein production with suspension‐adapted HEK293‐EBNA cells. Biotechnology and Bioengineering 75:2, pages 197-203.
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Marcel Hoffmann, Dennis Verzijl, Kenneth Lundstrom, Urs Simmen, Astrid E. Alewijnse, Henk Timmerman & Rob Leurs. (2001) Recombinant Semliki Forest virus for over-expression and pharmacological characterisation of the histamine H2 receptor in mammalian cells. European Journal of Pharmacology 427:2, pages 105-114.
Crossref
Kenneth Lundstrom, Christophe Schweitzer, Daniel Rotmann, Danielle Hermann, Edith M. Schneider & Markus U. Ehrengruber. (2001) Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery. FEBS Letters 504:3, pages 99-103.
Crossref
Kenneth Lundstrom. 2001. Animal Cell Technology: From Target to Market. Animal Cell Technology: From Target to Market 45 51 .
Wim Annaert & Bart De Strooper. (2000) Neuronal models to study amyloid precursor protein expression and processing in vitro. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease 1502:1, pages 53-62.
Crossref
C. Smerdou & P. Liljeström. 2000. Cell Engineering. Cell Engineering 182 210 .
Oleg ShamotienkoSobia AkhtarChristina SideraFrédéric A. MeunierBarbara InkMalcolm WeirJ. Oliver Dolly. (1999) Recreation of Neuronal Kv1 Channel Oligomers by Expression in Mammalian Cells Using Semliki Forest Virus. Biochemistry 38:51, pages 16766-16776.
Crossref
Derek E. Knight. (1999) Secretion from Bovine Chromaffin Cells Acutely Expressing Exogenous Proteins Using a Recombinant Semliki Forest Virus Containing an EGFP Reporter. Molecular and Cellular Neuroscience 14:6, pages 486-505.
Crossref
T. Wohland, K. Friedrich, R. Hovius & H. Vogel. (1999) Study of Ligand−Receptor Interactions by Fluorescence Correlation Spectroscopy with Different Fluorophores:  Evidence That the Homopentameric 5-Hydroxytryptamine Type 3 As Receptor Binds Only One Ligand . Biochemistry 38:27, pages 8671-8681.
Crossref
Ramachandran Arudchandran, Martin J. Brown, James S Song, Stephen A Wank, Hana Haleem-Smith & Juan Rivera. (1999) Polyethylene glycol-mediated infection of non-permissive mammalian cells with semliki forest virus: application to signal transduction studies. Journal of Immunological Methods 222:1-2, pages 197-208.
Crossref
Ana-Paula Tairi, Ruud Hovius, Horst Pick, Horst Blasey, Alain Bernard, Annmarie Surprenant, Kenneth Lundström & Horst Vogel. (1998) Ligand Binding to the Serotonin 5HT 3 Receptor Studied with a Novel Fluorescent Ligand . Biochemistry 37:45, pages 15850-15864.
Crossref
Kathryn Radford & Gary Buell. 1998. Ion Channels Part B. Ion Channels Part B 459 483 .
Kenneth Lundstrom. (1997) Alphaviruses as expression vectors. Current Opinion in Biotechnology 8:5, pages 578-582.
Crossref

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